LOCUS       sequence-382751-        2917 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pENTRY clone for NLR Integrated Domain ID#89.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Landry D, Mila I, Sabbagh CRR, Zaffuto M, Pouzet C, Tremousaygue D, 
            Dabos P, Deslandes L, Peeters N
  TITLE     An NLR integrated domain toolkit to identify plant pathogen effector
            targets.
  JOURNAL   Plant J. 2023 Sep;115(5):1443-1457. doi: 10.1111/tpj.16331. Epub 
            2023 Jul 24.
  PUBMED    37248633
REFERENCE   2  (bases 1 to 2917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.16331";
            journalName: "Plant J"; date: "2023-09"; volume: "115"; issue: "5"; 
            pages: "1443-1457"
COMMENT     Sequence Label: pIM46
FEATURES             Location/Qualifiers
     source          1..2917
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     68..90
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     82..99
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     83..99
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    152..251
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(804..903)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(960..976)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(960..976)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(973..995)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(1148..1736)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1228..1247)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1872..2687)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(1872..2687)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2595..2614
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2679..2698
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     2808..2824
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2808..2824
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     terminator      2825..2871
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 aacaaaaccc atcgtacggc caaggaagtc tccaataact gtgatccacc acaagcgcca
       61 gggttttccc agtcacgacg ttgtaaaacg acggccagtc atgcataatc cgcacgcatc
      121 tggaataagg aagtgccatt ccgcctgacc tcaaataatg attttatttt gactgatagt
      181 gacctgttcg ttgcaacaga ttgatgagca atgctttttt ataatgccaa ctttgtacaa
      241 aaaagcaggc ttcgaaggag atagaaccat gccaaactgc tgcggtgttg tgcatacaga
      301 tcacatcaca tttgaatcac agaacagtga ccatgaggtg aatgctggca tgctaatgca
      361 agctgttgtt gatacagata tgaggttcac aaacatttgg ttgggatcat caagtaacat
      421 gaaccagtcg agcattttgc atgactctgt gctcttcaag cactgcgaga aggatacttg
      481 gctgaatggt agcaagctga atttatcaga cggaaggcaa gttggagaat acataattgg
      541 tgatgcagga tttcctcttc ttccttggct tctcacatct ttccaggaga atgacctctc
      601 agattatcaa gtcgagttta ataggagaca ctctcaagcc atgaccatca cactgaccaa
      661 ggcactagcg aagttaaaag atacatggaa gttcctgcat ggaggggtgt ggcgcccaga
      721 aaatcaattt gagccgatgt gggtaatcta tgcatgttgc atgctgcata acatagtgat
      781 agacaaggag tgcggcacag gtgacccagc tttcttgtac aaagttggca ttataagaaa
      841 gcattgctta tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata aaatcattat
      901 ttgaggctag gtggaggctc agtgatgata agtctgcgat ggtggatgca tgtgtcatgg
      961 tcatagctgt ttcctgtgtg aaattgttat ccgctcagag ggcacaatcc tattccgcgc
     1021 tatccgacaa tctccaagac attaggtgga gttcagttcg gcgtatggca tatgtcgctg
     1081 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     1141 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     1201 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     1261 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     1321 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     1381 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     1441 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     1501 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     1561 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     1621 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     1681 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     1741 atcctttgat cttttctacg gggtctgacg ctctattcaa caaagccgcc gtcccgtcaa
     1801 gtcagcgtaa atgggtaggg ggcttcaaat cgtccgctct gccagtgtta caaccaatta
     1861 acaaattctg attagaaaaa ctcatcgagc atcaaatgaa actgcaattt attcatatca
     1921 ggattatcaa taccatattt ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg
     1981 aggcagttcc ataggatggc aagatcctgg tatcggtctg cgattccgac tcgtccaaca
     2041 tcaatacaac ctattaattt cccctcgtca aaaataaggt tatcaagtga gaaatcacca
     2101 tgagtgacga ctgaatccgg tgagaatggc aaaagcttat gcatttcttt ccagacttgt
     2161 tcaacaggcc agccattacg ctcgtcatca aaatcactcg catcaaccaa accgttattc
     2221 attcgtgatt gcgcctgagc gagacgaaat acgcgatcgc tgttaaaagg acaattacaa
     2281 acaggaatcg aatgcaaccg gcgcaggaac actgccagcg catcaacaat attttcacct
     2341 gaatcaggat attcttctaa tacctggaat gctgttttcc cggggatcgc agtggtgagt
     2401 aaccatgcat catcaggagt acggataaaa tgcttgatgg tcggaagagg cataaattcc
     2461 gtcagccagt ttagtctgac catctcatct gtaacatcat tggcaacgct acctttgcca
     2521 tgtttcagaa acaactctgg cgcatcgggc ttcccataca atcgatagat tgtcgcacct
     2581 gattgcccga cattatcgcg agcccattta tacccatata aatcagcatc catgttggaa
     2641 tttaatcgcg gcctcgagca agacgtttcc cgttgaatat ggctcataac accccttgta
     2701 ttactgttta tgtaagcaga cagttttatt gttcatgatg atatattttt atcttgtgca
     2761 atgtaacatc agagattttg agacacaacg tggctttccc ccgccgctct agaactagtg
     2821 gatccaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
     2881 gtcgcattat acgagacgtc caggttggga tacctga
//
