LOCUS       sequence-382752-        3592 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pENTRY clone for NLR Integrated Domain ID#14.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3592)
  AUTHORS   Landry D, Mila I, Sabbagh CRR, Zaffuto M, Pouzet C, Tremousaygue D, 
            Dabos P, Deslandes L, Peeters N
  TITLE     An NLR integrated domain toolkit to identify plant pathogen effector
            targets.
  JOURNAL   Plant J. 2023 Sep;115(5):1443-1457. doi: 10.1111/tpj.16331. Epub 
            2023 Jul 24.
  PUBMED    37248633
REFERENCE   2  (bases 1 to 3592)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.16331";
            journalName: "Plant J"; date: "2023-09"; volume: "115"; issue: "5"; 
            pages: "1443-1457"
COMMENT     Sequence Label: pIM65
FEATURES             Location/Qualifiers
     source          1..3592
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     68..90
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     82..99
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     83..99
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    152..251
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1479..1578)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1635..1651)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1635..1651)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1648..1670)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(1823..2411)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1903..1922)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2547..3362)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(2547..3362)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     3270..3289
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     3354..3373
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     3483..3499
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3483..3499
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     terminator      3500..3546
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 aacaaaaccc atcgtacggc caaggaagtc tccaataact gtgatccacc acaagcgcca
       61 gggttttccc agtcacgacg ttgtaaaacg acggccagtc atgcataatc cgcacgcatc
      121 tggaataagg aagtgccatt ccgcctgacc tcaaataatg attttatttt gactgatagt
      181 gacctgttcg ttgcaacaga ttgatgagca atgctttttt ataatgccaa ctttgtacaa
      241 aaaagcaggc ttcgaaggag atagaaccat ggatcatgtt gatggcgtta ccgatcaact
      301 gcaagtcaac attcagaaga ctaagttgga aatcaaacgc gaatacttat ttccaaaagt
      361 caacaaggat ggtattgttg tcaccgccaa atttgtgatc gatttcattg atactgttgt
      421 agtgaatctg ggtgatttat tgaaggtttt ttgttctagt tcactacttt ttgttcgtgg
      481 acctaacaaa gagatccgcg atgtttttaa ggagttgaag ttattgagaa attttgtatg
      541 cttcgttacg gacagattca aagagctgaa aagccaacct attgatttct tcgttcatgt
      601 tttagaggtg accagccacg ctgcaatgat tgcctggttg tatctcccaa gcaatgagaa
      661 agaaaatcag gaaacgaatg atttgctttc tgatcatttg aacatgaaga ttaagcccat
      721 tgaccgaagc atcggcaaga tctatattga tgtgttgcaa gctctacgat cagaatggcg
      781 tccgattatt tcaattgatc atgtagataa ttgtgtggct ggctttgtgg agactctcca
      841 acacaacttg aaggcaatat cggttagtaa tccaaatact catcaaatag cagaccttca
      901 ggagatgctc aacctcttaa ttgccaatct gtccatacaa gctcttgaat ttcaccttaa
      961 agatatcgac actgtgatga ttgattctgg aatactcgtt tactcgttgt gtgaagatgt
     1021 ggtgctaggg gaagtgagta ttgatttgcc aggtaccatt gagcgcatca agatattgat
     1081 ctacaacatt attcgaaagg agttccaatc tagtttgtca aggattcatg gactaggcta
     1141 tgttgatttc gccttaagca atcttcatga gttccaaaga cgatatgcag attcgcttga
     1201 ttctgtgaag actcaacttc aaataattca aactgaactg gatggcgtgc aaccttttct
     1261 gaagtttgtt gcagaacaac aatacaatat tcacgacaaa tttcagaata gtgtgtctct
     1321 actgattggc aaagcatatg aggtagaata tattgttgat gcttgtgtga gcaaaagagt
     1381 tccggactgg tgtctcatgc gttggctcgt ggacattagc tcagaggttg cagagatgca
     1441 acaaaagaaa atgtttgagg ttgacttagt gttacctgac ccagctttct tgtacaaagt
     1501 tggcattata agaaagcatt gcttatcaat ttgttgcaac gaacaggtca ctatcagtca
     1561 aaataaaatc attatttgag gctaggtgga ggctcagtga tgataagtct gcgatggtgg
     1621 atgcatgtgt catggtcata gctgtttcct gtgtgaaatt gttatccgct cagagggcac
     1681 aatcctattc cgcgctatcc gacaatctcc aagacattag gtggagttca gttcggcgta
     1741 tggcatatgt cgctggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     1801 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     1861 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     1921 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     1981 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     2041 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     2101 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     2161 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     2221 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     2281 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     2341 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     2401 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcta ttcaacaaag
     2461 ccgccgtccc gtcaagtcag cgtaaatggg tagggggctt caaatcgtcc gctctgccag
     2521 tgttacaacc aattaacaaa ttctgattag aaaaactcat cgagcatcaa atgaaactgc
     2581 aatttattca tatcaggatt atcaatacca tatttttgaa aaagccgttt ctgtaatgaa
     2641 ggagaaaact caccgaggca gttccatagg atggcaagat cctggtatcg gtctgcgatt
     2701 ccgactcgtc caacatcaat acaacctatt aatttcccct cgtcaaaaat aaggttatca
     2761 agtgagaaat caccatgagt gacgactgaa tccggtgaga atggcaaaag cttatgcatt
     2821 tctttccaga cttgttcaac aggccagcca ttacgctcgt catcaaaatc actcgcatca
     2881 accaaaccgt tattcattcg tgattgcgcc tgagcgagac gaaatacgcg atcgctgtta
     2941 aaaggacaat tacaaacagg aatcgaatgc aaccggcgca ggaacactgc cagcgcatca
     3001 acaatatttt cacctgaatc aggatattct tctaatacct ggaatgctgt tttcccgggg
     3061 atcgcagtgg tgagtaacca tgcatcatca ggagtacgga taaaatgctt gatggtcgga
     3121 agaggcataa attccgtcag ccagtttagt ctgaccatct catctgtaac atcattggca
     3181 acgctacctt tgccatgttt cagaaacaac tctggcgcat cgggcttccc atacaatcga
     3241 tagattgtcg cacctgattg cccgacatta tcgcgagccc atttataccc atataaatca
     3301 gcatccatgt tggaatttaa tcgcggcctc gagcaagacg tttcccgttg aatatggctc
     3361 ataacacccc ttgtattact gtttatgtaa gcagacagtt ttattgttca tgatgatata
     3421 tttttatctt gtgcaatgta acatcagaga ttttgagaca caacgtggct ttcccccgcc
     3481 gctctagaac tagtggatcc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     3541 ttttatctgt tgtttgtcgc attatacgag acgtccaggt tgggatacct ga
//
