LOCUS       sequence-382756-         991 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of myc-tagged human Nix with an alanine 
            substitution of serine-212. Based on Myc-Nix (#100795).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 991)
  AUTHORS   da Silva Rosa SC, Martens MD, Field JT, Nguyen L, Kereliuk SM, Hai 
            Y, Chapman D, Diehl-Jones W, Aliani M, West AR, Thliveris J, Ghavami
            S, Rampitsch C, Dolinsky VW, Gordon JW
  TITLE     BNIP3L/Nix-induced mitochondrial fission, mitophagy, and impaired 
            myocyte glucose uptake are abrogated by PRKA/PKA phosphorylation.
  JOURNAL   Autophagy. 2021 Sep;17(9):2257-2272. doi: 
            10.1080/15548627.2020.1821548. Epub 2020 Oct 12.
  PUBMED    33044904
REFERENCE   2  (bases 1 to 991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1080/15548627.2020.1821548"; journalName: "Autophagy"; date: 
            "2021-09"; volume: "17"; issue: "9"; pages: "2257-2272"
COMMENT     Sequence Label: Myc-Nix-S212A
FEATURES             Location/Qualifiers
     source          1..991
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(739..757)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(740..757)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(777..794)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    783..989
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gcagagctga tctccgagga ggacctggga tccatcgatg tcgacgaatt catgtcgtcc
       61 cacctagtcg agccgccgcc gcccctgcac aacaacaaca acaactgcga ggaaaatgag
      121 cagtctctgc ccccgccggc cggcctcaac agttcctggg tggagctacc catgaacagc
      181 agcaatggca atgataatgg caatgggaaa aatggggggc tggaacacgt accatcctca
      241 tcctccatcc acaatggaga catggagaag attcttttgg atgcacaaca tgaatcagga
      301 cagagtagtt ccagaggcag ttctcactgt gacagccctt cgccacaaga agatgggcag
      361 atcatgtttg atgtggaaat gcacaccagc agggaccata gctctcagtc agaagaagaa
      421 gttgtagaag gagagaagga agtcgaggct ttgaagaaaa gtgcggactg ggtatcagac
      481 tggtccagta gacccgaaaa cattccaccc aaggagttcc acttcagaca ccctaaacgt
      541 tctgtgtctt taagcatgag gaaaagtgga gccatgaaga aagggggtat tttctccgca
      601 gaatttctga aggtgttcat tccatctctc ttcctttctc atgttttggc tttggggcta
      661 ggcatctata ttggaaagcg actggccaca ccctctgcca gcacctactg actcgagcat
      721 gcatctagag ggccctattc tatagtgtca cctaaatgct agagctcgct gatcagcctc
      781 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
      841 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
      901 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
      961 ttgggaagan atagcaggca tgctggggat g
//
