LOCUS       sequence-382757-         973 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of myc-tagged human Nix with an aspartic acid 
            substitution of serine-212. Based on Myc-Nix (#100795).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 973)
  AUTHORS   da Silva Rosa SC, Martens MD, Field JT, Nguyen L, Kereliuk SM, Hai 
            Y, Chapman D, Diehl-Jones W, Aliani M, West AR, Thliveris J, Ghavami
            S, Rampitsch C, Dolinsky VW, Gordon JW
  TITLE     BNIP3L/Nix-induced mitochondrial fission, mitophagy, and impaired 
            myocyte glucose uptake are abrogated by PRKA/PKA phosphorylation.
  JOURNAL   Autophagy. 2021 Sep;17(9):2257-2272. doi: 
            10.1080/15548627.2020.1821548. Epub 2020 Oct 12.
  PUBMED    33044904
REFERENCE   2  (bases 1 to 973)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1080/15548627.2020.1821548"; journalName: "Autophagy"; date: 
            "2021-09"; volume: "17"; issue: "9"; pages: "2257-2272"
COMMENT     Sequence Label: Myc-Nix-S212D
FEATURES             Location/Qualifiers
     source          1..973
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(736..754)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(737..754)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(774..791)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    780..891
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gagctgatct ccgaggagga cctgggatcc atcgatgtcg acgaattcat gtcgtcccac
       61 ctagtcgagc cgccgccgcc cctgcacaac aacaacaaca actgcgagga aaatgagcag
      121 tctctgcccc cgccggccgg cctcaacagt tcctgggtgg agctacccat gaacagcagc
      181 aatggcaatg ataatggcaa tgggaaaaat ggggggctgg aacacgtacc atcctcatcc
      241 tccatccaca atggagacat ggagaagatt cttttggatg cacaacatga atcaggacag
      301 agtagttcca gaggcagttc tcactgtgac agcccttcgc cacaagaaga tgggcagatc
      361 atgtttgatg tggaaatgca caccagcagg gaccatagct ctcagtcaga agaagaagtt
      421 gtagaaggag agaaggaagt cgaggctttg aagaaaagtg cggactgggt atcagactgg
      481 tccagtagac ccgaaaacat tccacccaag gagttccact tcagacaccc taaacgttct
      541 gtgtctttaa gcatgaggaa aagtggagcc atgaagaaag ggggtatttt ctccgcagaa
      601 tttctgaagg tgttcattcc atctctcttc ctttctcatg ttttggcttt ggggctaggc
      661 atctatattg gaaagcgact ggacacaccc tctgccagca cctactgact cgagcatgca
      721 tctagagggc cctattctat agtgtcacct aaatgctaga gctcgctgat cagcctcgac
      781 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
      841 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
      901 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
      961 ggaagacaat agc
//
