LOCUS       sequence-382760-         984 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of myc-tagged mouse Bnip3 with an alanine 
            substitution of threonine-181. Based on Myc-Bnip3FL (#100796).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 984)
  AUTHORS   Martens MD, Seshadri N, Nguyen L, Chapman D, Henson ES, Xiang B, 
            Falk L, Mendoza A, Rattan S, Field JT, Kawalec P, Gibson SB, Keijzer
            R, Saleem A, Hatch GM, Doucette CA, Karch JM, Dolinsky VW, Dixon IM,
            West AR, Rampitsch C, Gordon JW
  TITLE     Misoprostol treatment prevents hypoxia-induced cardiac dysfunction 
            through a 14-3-3 and PKA regulatory motif on Bnip3.
  JOURNAL   Cell Death Dis. 2021 Nov 26;12(12):1105. doi: 
            10.1038/s41419-021-04402-3.
  PUBMED    34824192
REFERENCE   2  (bases 1 to 984)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Death 
            Dis."; date: "2021-11-26"; volume: "12(12):1105. doi"; pages: " 
            10.1038/s41419-021-04402-3"
COMMENT     Sequence Label: Myc-Bnip3-T181A
FEATURES             Location/Qualifiers
     source          1..984
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(643..661)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(644..661)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(681..698)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    687..911
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gcagagctga tctccgagga ggacctggga tccatcgatg tcgacgaatt catgtcgcag
       61 agcggggagg agaacctgca gggctcctgg gtagaactgc acttcagcaa tggcaatggg
      121 agcagcgttc cagcctccgt ctctatttat aatggtgaca tggaaaaaat actgttggat
      181 gcccagcatg aatctggacg aagtagctcc aagagttctc actgtgacag cccacctcgc
      241 tcccagacac cacaagatac caacagagct gaaatagaca gccacagctt tggcgagaaa
      301 aacagcactc tgtctgagga agattatatt gagagaagaa gagaagttga aagtatcctg
      361 aagaaaaact cagattggat atgggattgg tcaagtcgac cagaaaatat tccccccaag
      421 gagttccttt ttaaacaccc gaagcgcaca gctactctca gcatgagaaa cacaagcgtt
      481 atgaagaaag ggggaatttt ctcagcagac tttctgaagg ttttccttcc atctctgtta
      541 ctgtctcatc tgctggccat tggcttgggg atctacattg gaaggcgtct ggcaacttcc
      601 actagcacct tctgactcga gcatgcatct agagggccct attctatagt gtcacctaaa
      661 tgctagagct cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
      721 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
      781 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
      841 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
      901 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc
      961 gccctgtagc ggcgcattaa gcgc
//
