LOCUS       sequence-38277-e         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Zhao Y, Araki S, Wu J, Teramoto T, Chang YF, Nakano M, Abdelfattah 
            AS, Fujiwara M, Ishihara T, Nagai T, Campbell RE
  TITLE     An Expanded Palette of Genetically Encoded Ca2+ Indicators.
  JOURNAL   Science. 2011 Sep 30;333(6051):1888-91. doi: 10.1126/science.1208592
  PUBMED    21903779
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2011-09-30- 30"; volume: "333"; 
            issue: "6051"; pages: "1888-91"
COMMENT     Sequence Label: pTorPE-GEM-GECO1
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             72..83
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             238..255
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             259..291
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(295..303,304..318)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             346..402
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     615..636
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ttttatcgca actctctact gtttctccat acccgttttt tgggctaaca ggaggaatta
       61 accatgggtt taaagaggag aaaggtcatg aacaataacg atctctttca ggcatcacgt
      121 cggcgttttc tggcacaact cggcagctta accgacgccg ggacgctggg gccgtcattg
      181 ttaacgccgc gacgtgcgac tgcggcgcaa gcggcgactg acgcttctag aggttctcat
      241 catcatcatc atcatggtat ggctagcatg actggtggac agcaaatggg tcgggatctg
      301 tacgacgatg atgataagga tctcgccaca atggtcgact catcacgtcg taagtggaat
      361 aagacaggtc acgcagtcag agctataggt cggctgagct caccagagaa cgtgtatata
      421 aaggccgacg agcagaagaa cggcatcaag gcgtacttca agatccgcca caacatcgag
      481 ggcggcggcg tgcagctcgc ctaccactac cagcagatca cccccatagg cgacggcccc
      541 gtgctgctgc ccgacaacca ctacctgagc gtgcagtcca tactttcgaa agaccccaac
      601 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
      661 atggacgagc tgtacaaggg tggcagcggt ggcatggtga gcaagggcga ggagctgttc
      721 accggggtgg tgcccatcca ggtcgagctg gacggcgacg taaacggcca caagttcagc
      781 gtgtccggcg agggtgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
      841 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgtc ctacggcgtg
      901 cagtgcttca gccgctaccc
//
