LOCUS       sequence-382803-       12162 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR, Cas9 driven by double 35S, nptII for plant selection, KpnI 
            site for gRNA insertion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12162)
  AUTHORS   Ortega MA, Zhou R, Chen MSS, Bewg WP, Simon B, Tsai CJ
  TITLE     In vitro floral development in poplar: insights into seed trichome 
            regulation and trimonoecy.
  JOURNAL   New Phytol. 2022 Nov 16. doi: 10.1111/nph.18624.
  PUBMED    36385612
REFERENCE   2  (bases 1 to 12162)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "New Phytol.
            2022 Nov 16. doi: 10.1111/nph.18624."
COMMENT     Sequence Label: pORE303N
FEATURES             Location/Qualifiers
     source          1..12162
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    21..45
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(560..1354)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(671..690)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     1281..1300
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     misc_RNA        2649..2724
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        2821..3497
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     3434..3454
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             3543..7646
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             3543..7646
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             3543..7646
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     terminator      7686..7934
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_feature    7953..7977
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      8191..8779
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8680..8699
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8933..8950
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(9019..10167)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MAQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(10466..11260)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHLERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      11472..12103
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 gggagctgtt ggctggctgg tggcaggata tattgtggtg taaacaaatt gacgatttaa
       61 atggtaggcg cggcttaatt aagcgcttaa ttaagcgctt aattaagcgc ttaataagcc
      121 gcgccgtacc ccaatagtct cgacagacac atagcaccta acattattgc ttaaatttac
      181 tattttttta ctatattata ctcaacccaa tgagcataaa gactgtaaaa tctcaaattc
      241 ctgagaagca tatttatcga tcccacagac ttgatagttc cataatccat acgctgcagc
      301 caaattgcta gtgtgttgaa catttaacac gtagagaact agaaaagata taaaactaag
      361 attgatatcc aaaatagacg agaacaataa gcaaaaactc ttagttttga aataaatcaa
      421 caatcccgag ggttgtcaca tacatcaaaa acgaaaatcc atatagcaaa aaaaactcta
      481 aattaccgtt cgacaaaaag agaaaactga taggacattt gctaaacatt aaaatcaaaa
      541 tttggatcaa gccgaattct cagaagaact cgtcaagaag gcgatagaag gcgatgcgct
      601 gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa
      661 gctcttcagc aatatcacgg gtagccaacg ctatgtcctg atagcggtcc gccacaccca
      721 gccggccaca gtcgatgaat ccagaaaagc ggccattttc caccatgata ttcggcaagc
      781 aggcatcgcc atgggtcacg acgagatcat cgccgtcggg catgcgcgcc ttgagcctgg
      841 cgaacagttc ggctggcgcg agcccctgat gctcttcgtc cagatcatcc tgatcgacaa
      901 gaccggcttc catccgagta cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg
      961 ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc atcagccatg atggatactt
     1021 tctcggcagg agcaaggtga gatgacagga gatcctgccc cggcacttcg cccaatagca
     1081 gccagtccct tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg
     1141 tggccagcca cgatagccgc gctgcctcgt cctgcagttc attcagggca ccggacaggt
     1201 cggtcttgac aaaaagaacc gggcgcccct gcgctgacag ccggaacacg gcggcatcag
     1261 agcagccgat tgtctgttgt gcccagtcat agccgaatag cctctccacc caagcggccg
     1321 gagaacctgc gtgcaatcca tcttgttcaa tcatctcgag acgtaccgcg cgatgcattc
     1381 gaagatcgat ccttcgcctg gaggagagaa atcagtggcg ctgcggcttt tagggtttct
     1441 ttgttgatgg aatgagagtg taagctctgc cagtgccact ttattagggt tttacaagcc
     1501 cttttcttcg taattgggcc tgacattttg tgccacttgg gcctttagag atgaaaatgt
     1561 atattgggct taagttgact tgaaggataa attagtttag gatattacgt tttttatgag
     1621 aattggtgtg tcggatacat gtatatgatg cattcaaata tatgtattct agatacattt
     1681 aagtttagat acaatctaaa atgtgtcttt aattacagga ctgtaactaa aatacttaat
     1741 gtaagaagaa tattactcct ttaatagctt ttgagtatat ctagtctaac atcttttaaa
     1801 aaagtctaat ttctttcatt tatttttcga gcaatagcaa agtgcataat tatttttttc
     1861 ttctagaaat tcagatttgt ttctctaaat tttgagattc ttttctcaat tttgtatgtc
     1921 tagagaacaa tgtgtatttt tcactctagt tggttgttac tttgttgaat gttctgataa
     1981 aagtatattg ttatttctga agtagatata aaccttcatt tggaaattat acataaatca
     2041 aaatcgttaa ttatctagat caagatatat gcccttttcc taatgtattt gatacatgca
     2101 cctaatttca ctagatgtat cttttctatt ttttaaatta tgaatagtta attttttcca
     2161 tatgtgtatt tgatacatac ttcatgactt taaaaaatta attatatacc agatatatgt
     2221 atttaaaatt tgttatgtat ttaaagtatg tatatgatta ttcgatatta atctcttcga
     2281 tgaacctagg atgcctatct tatatgatca atgaggcatt taattgggtg catatgatgg
     2341 tgaaaaaagg tgcagctcct ggcttgggaa tgatgactca tgtggaattt ggtcttaaat
     2401 ttatcacatc cttttgggat gtgatgattg tatcacttgt tcattttgca aagacaaggt
     2461 gcactgctac aaactttggt ttaatctgaa ataaaacaaa actcactgag aggaagatgc
     2521 atcccagtag gtgaaagtcg agaaggattt gcatgttact attacacttg ctttttagtc
     2581 ccacatcgtc tgaaacataa aatatttcag cgtttaaata cttcaagcga accagtaggc
     2641 ttggtaccgt tttagagcta gaaatagcaa gttaaaataa ggctagtccg ttatcaactt
     2701 gaaaaagtgg caccgagtcg gtgctttttt tactagttac gggcccaaca tggtggagca
     2761 cgacactctc gtctactcca agaatatcaa agatacagtc tcagaagacc aaagggctat
     2821 tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt gcccagctat
     2881 ctgtcacttc atcaaaagga cagtagaaaa ggaaggtggc acctacaaat gccatcattg
     2941 cgataaagga aaggctatcg ttcaagatgc ctctgccgac agtggtccca aagatggacc
     3001 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
     3061 ggattgatgt gaacatggtg gagcacgaca ctctcgtcta ctccaagaat atcaaagata
     3121 cagtctcaga agaccaaagg gctattgaga cttttcaaca aagggtaata tcgggaaacc
     3181 tcctcggatt ccattgccca gctatctgtc acttcatcaa aaggacagta gaaaaggaag
     3241 gtggcaccta caaatgccat cattgcgata aaggaaaggc tatcgttcaa gatgcctctg
     3301 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg
     3361 ttccaaccac gtcttcaaag caagtggatt gatgtgatat ctccactgac gtaagggatg
     3421 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt
     3481 tggagaggac acgctgaaat caccagtctc tctctacaaa tctatctctc tcgaccgcta
     3541 gcatggacaa gaagtactcc attgggctcg atatcggcac aaacagcgtc ggctgggccg
     3601 tcattacgga cgagtacaag gtgccgagca aaaaattcaa agttctgggc aataccgatc
     3661 gccacagcat aaagaagaac ctcattggcg ccctcctgtt cgactccggg gagacggccg
     3721 aagccacgcg gctcaaaaga acagcacggc gcagatatac ccgcagaaag aatcggatct
     3781 gctacctgca ggagatcttt agtaatgaga tggctaaggt ggatgactct ttcttccata
     3841 ggctggagga gtcctttttg gtggaggagg ataaaaagca cgagcgccac ccaatctttg
     3901 gcaatatcgt ggacgaggtg gcgtaccatg aaaagtaccc aaccatatat catctgagga
     3961 agaagcttgt agacagtact gataaggctg acttgcggtt gatctatctc gcgctggcgc
     4021 atatgatcaa atttcgggga cacttcctca tcgaggggga cctgaaccca gacaacagcg
     4081 atgtcgacaa actctttatc caactggttc agacttacaa tcagcttttc gaagagaacc
     4141 cgatcaacgc atccggagtt gacgccaaag caatcctgag cgctaggctg tccaaatccc
     4201 ggcggctcga aaacctcatc gcacagctcc ctggggagaa gaagaacggc ctgtttggta
     4261 atcttatcgc cctgtcactc gggctgaccc ccaactttaa atctaacttc gacctggccg
     4321 aagatgccaa gcttcaactg agcaaagaca cctacgatga tgatctcgac aatctgctgg
     4381 cccagatcgg cgaccagtac gcagaccttt ttttggcggc aaagaacctg tcagacgcca
     4441 ttctgctgag tgatattctg cgagtgaaca cggagatcac caaagctccg ctgagcgcta
     4501 gtatgatcaa gcgctatgat gagcaccacc aagacttgac tttgctgaag gcccttgtca
     4561 gacagcaact gcctgagaag tacaaggaaa ttttcttcga tcagtctaaa aatggctacg
     4621 ccggatacat tgacggcgga gcaagccagg aggaatttta caaatttatt aagcccatct
     4681 tggaaaaaat ggacggcacc gaggagctgc tggtaaagct taacagagaa gatctgttgc
     4741 gcaaacagcg cactttcgac aatggaagca tcccccacca gattcacctg ggcgaactgc
     4801 acgctatcct caggcggcaa gaggatttct accccttttt gaaagataac agggaaaaga
     4861 ttgagaaaat cctcacattt cggataccct actatgtagg ccccctcgcc cggggaaatt
     4921 ccagattcgc gtggatgact cgcaaatcag aagagaccat cactccctgg aacttcgagg
     4981 aagtcgtgga taagggggcc tctgcccagt ccttcatcga aaggatgact aactttgata
     5041 aaaatctgcc taacgaaaag gtgcttccta aacactctct gctgtacgag tacttcacag
     5101 tttataacga gctgaccaag gtcaaatacg tcacagaagg gatgagaaag ccagcattcc
     5161 tgtctggaga gcagaagaaa gctatcgtgg acctcctctt caagacgaac cggaaagtta
     5221 ccgtgaaaca gctcaaagaa gactatttca aaaagattga atgtttcgac tctgttgaaa
     5281 tcagcggagt ggaggatcgc ttcaacgcat ccctgggaac gtatcacgat ctcctgaaaa
     5341 tcattaaaga caaggacttc ctggacaatg aggagaacga ggacattctt gaggacattg
     5401 tcctcaccct tacgttgttt gaagataggg agatgattga agaacgcttg aaaacttacg
     5461 ctcatctctt cgacgacaaa gtcatgaaac agctcaagag gcgccgatat acaggatggg
     5521 ggcggctgtc aagaaaactg atcaatggca tccgagacaa gcagagtgga aagacaatcc
     5581 tggattttct taagtccgat ggatttgcca accggaactt catgcagttg atccatgatg
     5641 actctctcac ctttaaggag gacatccaga aagcacaagt ttctggccag ggggacagtc
     5701 ttcacgagca catcgctaat cttgcaggta gcccagctat caaaaaggga atactgcaga
     5761 ccgttaaggt cgtggatgaa ctcgtcaaag taatgggaag gcataagccc gagaatatcg
     5821 ttatcgagat ggcccgagag aaccaaacta cccagaaggg acagaagaac agtagggaaa
     5881 ggatgaagag gattgaagag ggtataaaag aactggggtc ccaaatcctt aaggaacacc
     5941 cagttgaaaa cacccagctt cagaatgaga agctctacct gtactacctg cagaacggca
     6001 gggacatgta cgtggatcag gaactggaca tcaatcggct ctccgactac gacgtggatc
     6061 atatcgtgcc ccagtctttt ctcaaagatg attctattga taataaagtg ttgacaagat
     6121 ccgataaaaa tagagggaag agtgataacg tcccctcaga agaagttgtc aagaaaatga
     6181 aaaattattg gcggcagctg ctgaacgcca aactgatcac acaacggaag ttcgataatc
     6241 tgactaaggc tgaacgaggt ggcctgtctg agttggataa agccggcttc atcaaaaggc
     6301 agcttgttga gacacgccag atcaccaagc acgtggccca aattctcgat tcacgcatga
     6361 acaccaagta cgatgaaaat gacaaactga ttcgagaggt gaaagttatt actctgaagt
     6421 ctaagctggt ctcagatttc agaaaggact ttcagtttta taaggtgaga gagatcaaca
     6481 attaccacca tgcgcatgat gcctacctga atgcagtggt aggcactgca cttatcaaaa
     6541 aatatcccaa gcttgaatct gaatttgttt acggagacta taaagtgtac gatgttagga
     6601 aaatgatcgc aaagtctgag caggaaatag gcaaggccac cgctaagtac ttcttttaca
     6661 gcaatattat gaattttttc aagaccgaga ttacactggc caatggagag attcggaagc
     6721 gaccacttat cgaaacaaac ggagaaacag gagaaatcgt gtgggacaag ggtagggatt
     6781 tcgcgacagt ccggaaggtc ctgtccatgc cgcaggtgaa catcgttaaa aagaccgaag
     6841 tacagaccgg aggcttctcc aaggaaagta tcctcccgaa aaggaacagc gacaagctga
     6901 tcgcacgcaa aaaagattgg gaccccaaga aatacggcgg attcgattct cctacagtcg
     6961 cttacagtgt actggttgtg gccaaagtgg agaaagggaa gtctaaaaaa ctcaaaagcg
     7021 tcaaggaact gctgggcatc acaatcatgg agcgatcaag cttcgaaaaa aaccccatcg
     7081 actttctcga agcgaaagga tataaagagg tcaaaaaaga cctcatcatt aagcttccca
     7141 agtactctct ctttgagctt gaaaacggcc ggaaacgaat gctcgctagt gcgggcgagc
     7201 tgcagaaagg taacgagctg gcactgccct ctaaatacgt taatttcttg tatctggcca
     7261 gccactatga aaagctcaaa gggtctcccg aagataatga gcagaagcag ctgttcgtgg
     7321 aacaacacaa acactacctt gatgagatca tcgagcaaat aagcgagttc tccaaaagag
     7381 tgatcctcgc cgacgctaac ctcgataagg tgctttctgc ttacaataag cacagggata
     7441 agcccatcag ggagcaggca gaaaacatta tccacttgtt tactctgacc aacttgggcg
     7501 cgcctgcagc cttcaagtac ttcgacacca ccatagacag aaagcggtac acctctacaa
     7561 aggaggtcct ggacgccaca ctgattcatc agtcaattac ggggctctat gaaacaagaa
     7621 tcgacctctc tcagctcggt ggagacggaa caagaaagag aagaaaggtg tgacggatcc
     7681 tcgaatatga agatgaagat gaaatatttg gtgtgtcaaa taaaaagctt gtgtgcttaa
     7741 gtttgtgttt ttttcttggc ttgttgtgtt atgaatttgt ggctttttct aatatcaaat
     7801 gaatgtaaga tctcattata atgaataaac aaatgtttct ataatccatt gtgaatgttt
     7861 tgttggatct cttctgcagc atataactac tgtatgtgct atggtatgga ctatggaata
     7921 tgattaaaga taaggtttaa actatcagtg tttgacagga tatattggcg ggtaaaccta
     7981 agagaaaaga gcgtttatta gaataatcgg atatttaaaa gggcgtgaaa aggtttatcc
     8041 gttcgtccat ttgtatgtgc atgccaacca caggagatcc aaaaggatct aggtgaagat
     8101 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     8161 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     8221 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     8281 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     8341 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     8401 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     8461 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     8521 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     8581 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     8641 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     8701 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     8761 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     8821 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     8881 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     8941 agtgagcgag gaagcggaag aggatctcag taaagcgctg gctgctgaac ccccagccgg
     9001 aactgacccc acaaggccct agcgtttgca atgcaccagg tcatcattga cccaggcgtg
     9061 ttccaccagg ccgctgcctc gcaactcttc gcaggcttcg ccgacctgct cgcgccactt
     9121 cttcacgcgg gtggaatccg atccgcacat gaggcggaag gtttccagct tgagcgggta
     9181 cggctcccgg tgcgagctga aatagtcgaa catccgtcgg gccgtcggcg acagcttgcg
     9241 gtacttctcc catatgaatt tcgtgtagtg gtcgccagca aacagcacga cgatttcctc
     9301 gtcgatcagg acctggcaac gggacgtttt cttgccacgg tccaggacgc ggaagcggtg
     9361 cagcagcgac accgattcca ggtgcccaac gcggtcggac gtgaagccca tcgccgtcgc
     9421 ctgtaggcgc gacaggcatt cctcggcctt cgtgtaatac cggccattga tcgaccagcc
     9481 caggtcctgg caaagctcgt agaacgtgaa ggtgatcggc tcgccgatag gggtgcgctt
     9541 cgcgtactcc aacacctgct gccacaccag ttcgtcatcg tcggcccgca gctcgacgcc
     9601 ggtgtaggtg atcttcacgt ccttgttgac gtggaaaatg accttgtttt gcagcgcctc
     9661 gcgcgggatt ttcttgttgc gcgtggtgaa cagggcagag cgggccgtgt cgtttggcat
     9721 cgctcgcatc gtgtccggcc acggcgcaat atcgaacaag gaaagctgca tttccttgat
     9781 ctgctgcttc gtgtgtttca gcaacgcggc ttgcttggcc tcgctgacct gttttgccag
     9841 gtcctcgccg gcggtttttc gcttcttggt cgtcatagtt cctcgcgtgt cgatggtcat
     9901 cgacttcgcc aaacctgccg cctcctgttc gagacgacgc gaacgctcca cggcggccga
     9961 tggcgcgggc agggcagggg gagccagttg cacgctgtcg cgctcgatct tggccgtagc
    10021 ttgctgggcc atcgagccga cggactggaa ggtttcgcgg ggcgcacgca tgacggtgcg
    10081 gcttgcgatg gtttcggcat cctcggcgga aaaccccgcg tcgatcagtt cttgcctgta
    10141 tgccttccgg tcaaacgtcc gattcattca ccctccttgc gggattgccc cgactcacgc
    10201 cggggcaatg tgcccttatt cctgatttga tccgcctggt gccttggtgt ccagataatc
    10261 caccttatcg gcaatgaagt cggtcccgta gaccgtctgg ccgtccttct cgtacttggt
    10321 attccgaatc ttgccctgca cgaataccag cgaccccttg cccaaatact tgccgtgggc
    10381 ctcggcctga gagccaaaac acttgatgcg gaagaagtcg gtgcgctcct gcttgtcgcc
    10441 agcatcgttg cgccacatct aggtactaaa acaattcatc cagtaaaata taatatttta
    10501 ttttctccca atcaggcttg atccccagta agtcaaaaaa tagctcgaca tactgttctt
    10561 ccccaatatc ctccctgatc gaccggacgc agaaggcaat gtcataccac ttgtccgccc
    10621 tgccgcttct cccaagatca ataaagccac ttactttgcc atctttcaca aagatgttgc
    10681 tgtctcccag gtcgccgtgg gaaaagacaa gttcctcttc gggcttttcc gtctttaaaa
    10741 aatcatacag ctcgcgcgga tctttaaatg gggtgtcttc ttcccagttt tcgcaatcca
    10801 catcggccag atcgttattc agtaagtaat ccaattcggc taagcggctg tctaagctat
    10861 tcgtataggg acaatccgat atgtcgatgg agtgaaagag cctgatgcac tccgcataca
    10921 gctcgataat cttttcaggg ctttgttcat cttcatactc ttccgagcaa aggacgccat
    10981 cggcctcact catgagcaga ttgctccagc catcatgccg ttcaaggtgc aggacctttg
    11041 gaacaggcag ctttccttcc agccatagca tcatgtcctt ttcccgttcc acatcatagg
    11101 tggtcccttt ataccggctg tccgtcattt ttaaatatag gttttcattt tctcccacca
    11161 gcttatatac cttagcagga gacattcctt ccgtatcttt tacgcagcgg tatttttcga
    11221 tcagtttttt caattccggt gatattctca ttttagccat ttattatttc cttcctcttt
    11281 tctacagtat ttaaagatac cccaagaagc taattataac aagacgaact ccaattcact
    11341 gttccttgca ttctaaaact ttaaatacca gaaaacagct ttttcaaagt tgttttcaaa
    11401 gttggcgtat aacatagtat cgacggagcc gattttgaaa ccacaattat gggtgatgct
    11461 gccaacgcga gagcgggccg ggagggttcg agaagggggg gcacccccct tcggcgtgcg
    11521 cggtcacgcg cacagggcgc agccctggtt aaaaacaagg tttataaata ttggtttaaa
    11581 agcaggttaa aagacaggtt agcggtggcc gaaaaacggg cggaaaccct tgcaaatgct
    11641 ggattttctg cctgtggaca gcccctcaaa tgtcaatagg tgcgcccctc atctgtcagc
    11701 actctgcccc tcaagtgtca aggatcgcgc ccctcatctg tcagtagtcg cgcccctcaa
    11761 gtgtcaatac cgcagggcac ttatccccag gcttgtccac atcatctgtg ggaaactcgc
    11821 gtaaaatcag gcgttttcgc cgatttgcga ggctggccag ctccacgtcg ccggccgaaa
    11881 tcgagcctgc ccctcatctg tcaacgccgc gccgggtgag tcggcccctc aagtgtcaac
    11941 gtccgcccct catctgtcag tgagggccaa gttttccgcg aggtatccac aacgccggcg
    12001 gctgcggtgt ctcgcacacg gcttcgacgg cgtttctggc gcgtttgcag ggccatagac
    12061 ggccgccagc ccagcggcga gggcaaccag cccggtgagc gtctctagtg gactgatggg
    12121 ctgcctgtat cgagtggtga ttttgtgccg agctgccggt cg
//
