LOCUS       sequence-382806-       12176 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR, Cas9 driven by double 35S, nptII for plant selection, 
            knockout CEN1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12176)
  AUTHORS   Ortega MA, Zhou R, Chen MSS, Bewg WP, Simon B, Tsai CJ
  TITLE     In vitro floral development in poplar: insights into seed trichome 
            regulation and trimonoecy.
  JOURNAL   New Phytol. 2022 Nov 16. doi: 10.1111/nph.18624.
  PUBMED    36385612
REFERENCE   2  (bases 1 to 12176)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "New Phytol.
            2022 Nov 16. doi: 10.1111/nph.18624."
COMMENT     Sequence Label: pORE303N_CEN1
FEATURES             Location/Qualifiers
     source          1..12176
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(522..1316)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(633..652)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     1243..1262
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     misc_RNA        2625..2700
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        2797..3473
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     3410..3430
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             3519..7622
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             3519..7622
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             3519..7622
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     terminator      7662..7910
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_feature    7929..7953
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      8167..8755
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8656..8675
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8909..8926
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(8995..10143)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MAQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(10442..11236)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHLERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      11448..12079
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    join(12159..12176,1..7)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 tgtaaacaaa ttgacgattt aaatggtagg cgcggcttaa ttaagcgctt aattaagcgc
       61 ttaattaagc gcttaataag ccgcgccgta ccccaatagt ctcgacagac acatagcacc
      121 taacattatt gcttaaattt actatttttt tactatatta tactcaaccc aatgagcata
      181 aagactgtaa aatctcaaat tcctgagaag catatttatc gatcccacag acttgatagt
      241 tccataatcc atacgctgca gccaaattgc tagtgtgttg aacatttaac acgtagagaa
      301 ctagaaaaga tataaaacta agattgatat ccaaaataga cgagaacaat aagcaaaaac
      361 tcttagtttt gaaataaatc aacaatcccg agggttgtca catacatcaa aaacgaaaat
      421 ccatatagca aaaaaaactc taaattaccg ttcgacaaaa agagaaaact gataggacat
      481 ttgctaaaca ttaaaatcaa aatttggatc aagccgaatt ctcagaagaa ctcgtcaaga
      541 aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag
      601 cggtcagccc attcgccgcc aagctcttca gcaatatcac gggtagccaa cgctatgtcc
      661 tgatagcggt ccgccacacc cagccggcca cagtcgatga atccagaaaa gcggccattt
      721 tccaccatga tattcggcaa gcaggcatcg ccatgggtca cgacgagatc atcgccgtcg
      781 ggcatgcgcg ccttgagcct ggcgaacagt tcggctggcg cgagcccctg atgctcttcg
      841 tccagatcat cctgatcgac aagaccggct tccatccgag tacgtgctcg ctcgatgcga
      901 tgtttcgctt ggtggtcgaa tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt
      961 gcatcagcca tgatggatac tttctcggca ggagcaaggt gagatgacag gagatcctgc
     1021 cccggcactt cgcccaatag cagccagtcc cttcccgctt cagtgacaac gtcgagcaca
     1081 gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc gcgctgcctc gtcctgcagt
     1141 tcattcaggg caccggacag gtcggtcttg acaaaaagaa ccgggcgccc ctgcgctgac
     1201 agccggaaca cggcggcatc agagcagccg attgtctgtt gtgcccagtc atagccgaat
     1261 agcctctcca cccaagcggc cggagaacct gcgtgcaatc catcttgttc aatcatctcg
     1321 agacgtaccg cgcgatgcat tcgaagatcg atccttcgcc tggaggagag aaatcagtgg
     1381 cgctgcggct tttagggttt ctttgttgat ggaatgagag tgtaagctct gccagtgcca
     1441 ctttattagg gttttacaag cccttttctt cgtaattggg cctgacattt tgtgccactt
     1501 gggcctttag agatgaaaat gtatattggg cttaagttga cttgaaggat aaattagttt
     1561 aggatattac gttttttatg agaattggtg tgtcggatac atgtatatga tgcattcaaa
     1621 tatatgtatt ctagatacat ttaagtttag atacaatcta aaatgtgtct ttaattacag
     1681 gactgtaact aaaatactta atgtaagaag aatattactc ctttaatagc ttttgagtat
     1741 atctagtcta acatctttta aaaaagtcta atttctttca tttatttttc gagcaatagc
     1801 aaagtgcata attatttttt tcttctagaa attcagattt gtttctctaa attttgagat
     1861 tcttttctca attttgtatg tctagagaac aatgtgtatt tttcactcta gttggttgtt
     1921 actttgttga atgttctgat aaaagtatat tgttatttct gaagtagata taaaccttca
     1981 tttggaaatt atacataaat caaaatcgtt aattatctag atcaagatat atgccctttt
     2041 cctaatgtat ttgatacatg cacctaattt cactagatgt atcttttcta ttttttaaat
     2101 tatgaatagt taattttttc catatgtgta tttgatacat acttcatgac tttaaaaaat
     2161 taattatata ccagatatat gtatttaaaa tttgttatgt atttaaagta tgtatatgat
     2221 tattcgatat taatctcttc gatgaaccta ggatgcctat cttatatgat caatgaggca
     2281 tttaattggg tgcatatgat ggtgaaaaaa ggtgcagctc ctggcttggg aatgatgact
     2341 catgtggaat ttggtcttaa atttatcaca tccttttggg atgtgatgat tgtatcactt
     2401 gttcattttg caaagacaag gtgcactgct acaaactttg gtttaatctg aaataaaaca
     2461 aaactcactg agaggaagat gcatcccagt aggtgaaagt cgagaaggat ttgcatgtta
     2521 ctattacact tgctttttag tcccacatcg tctgaaacat aaaatatttc agcgtttaaa
     2581 tacttcaagc gaaccagtag gcttgtatca gtccagcagg aagcgtttta gagctagaaa
     2641 tagcaagtta aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc
     2701 tttttttact agttacgggc ccaacatggt ggagcacgac actctcgtct actccaagaa
     2761 tatcaaagat acagtctcag aagaccaaag ggctattgag acttttcaac aaagggtaat
     2821 atcgggaaac ctcctcggat tccattgccc agctatctgt cacttcatca aaaggacagt
     2881 agaaaaggaa ggtggcacct acaaatgcca tcattgcgat aaaggaaagg ctatcgttca
     2941 agatgcctct gccgacagtg gtcccaaaga tggaccccca cccacgagga gcatcgtgga
     3001 aaaagaagac gttccaacca cgtcttcaaa gcaagtggat tgatgtgaac atggtggagc
     3061 acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac caaagggcta
     3121 ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat tgcccagcta
     3181 tctgtcactt catcaaaagg acagtagaaa aggaaggtgg cacctacaaa tgccatcatt
     3241 gcgataaagg aaaggctatc gttcaagatg cctctgccga cagtggtccc aaagatggac
     3301 ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag
     3361 tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac tatccttcgc
     3421 aagacccttc ctctatataa ggaagttcat ttcatttgga gaggacacgc tgaaatcacc
     3481 agtctctctc tacaaatcta tctctctcga ccgctagcat ggacaagaag tactccattg
     3541 ggctcgatat cggcacaaac agcgtcggct gggccgtcat tacggacgag tacaaggtgc
     3601 cgagcaaaaa attcaaagtt ctgggcaata ccgatcgcca cagcataaag aagaacctca
     3661 ttggcgccct cctgttcgac tccggggaga cggccgaagc cacgcggctc aaaagaacag
     3721 cacggcgcag atatacccgc agaaagaatc ggatctgcta cctgcaggag atctttagta
     3781 atgagatggc taaggtggat gactctttct tccataggct ggaggagtcc tttttggtgg
     3841 aggaggataa aaagcacgag cgccacccaa tctttggcaa tatcgtggac gaggtggcgt
     3901 accatgaaaa gtacccaacc atatatcatc tgaggaagaa gcttgtagac agtactgata
     3961 aggctgactt gcggttgatc tatctcgcgc tggcgcatat gatcaaattt cggggacact
     4021 tcctcatcga gggggacctg aacccagaca acagcgatgt cgacaaactc tttatccaac
     4081 tggttcagac ttacaatcag cttttcgaag agaacccgat caacgcatcc ggagttgacg
     4141 ccaaagcaat cctgagcgct aggctgtcca aatcccggcg gctcgaaaac ctcatcgcac
     4201 agctccctgg ggagaagaag aacggcctgt ttggtaatct tatcgccctg tcactcgggc
     4261 tgacccccaa ctttaaatct aacttcgacc tggccgaaga tgccaagctt caactgagca
     4321 aagacaccta cgatgatgat ctcgacaatc tgctggccca gatcggcgac cagtacgcag
     4381 accttttttt ggcggcaaag aacctgtcag acgccattct gctgagtgat attctgcgag
     4441 tgaacacgga gatcaccaaa gctccgctga gcgctagtat gatcaagcgc tatgatgagc
     4501 accaccaaga cttgactttg ctgaaggccc ttgtcagaca gcaactgcct gagaagtaca
     4561 aggaaatttt cttcgatcag tctaaaaatg gctacgccgg atacattgac ggcggagcaa
     4621 gccaggagga attttacaaa tttattaagc ccatcttgga aaaaatggac ggcaccgagg
     4681 agctgctggt aaagcttaac agagaagatc tgttgcgcaa acagcgcact ttcgacaatg
     4741 gaagcatccc ccaccagatt cacctgggcg aactgcacgc tatcctcagg cggcaagagg
     4801 atttctaccc ctttttgaaa gataacaggg aaaagattga gaaaatcctc acatttcgga
     4861 taccctacta tgtaggcccc ctcgcccggg gaaattccag attcgcgtgg atgactcgca
     4921 aatcagaaga gaccatcact ccctggaact tcgaggaagt cgtggataag ggggcctctg
     4981 cccagtcctt catcgaaagg atgactaact ttgataaaaa tctgcctaac gaaaaggtgc
     5041 ttcctaaaca ctctctgctg tacgagtact tcacagttta taacgagctg accaaggtca
     5101 aatacgtcac agaagggatg agaaagccag cattcctgtc tggagagcag aagaaagcta
     5161 tcgtggacct cctcttcaag acgaaccgga aagttaccgt gaaacagctc aaagaagact
     5221 atttcaaaaa gattgaatgt ttcgactctg ttgaaatcag cggagtggag gatcgcttca
     5281 acgcatccct gggaacgtat cacgatctcc tgaaaatcat taaagacaag gacttcctgg
     5341 acaatgagga gaacgaggac attcttgagg acattgtcct cacccttacg ttgtttgaag
     5401 atagggagat gattgaagaa cgcttgaaaa cttacgctca tctcttcgac gacaaagtca
     5461 tgaaacagct caagaggcgc cgatatacag gatgggggcg gctgtcaaga aaactgatca
     5521 atggcatccg agacaagcag agtggaaaga caatcctgga ttttcttaag tccgatggat
     5581 ttgccaaccg gaacttcatg cagttgatcc atgatgactc tctcaccttt aaggaggaca
     5641 tccagaaagc acaagtttct ggccaggggg acagtcttca cgagcacatc gctaatcttg
     5701 caggtagccc agctatcaaa aagggaatac tgcagaccgt taaggtcgtg gatgaactcg
     5761 tcaaagtaat gggaaggcat aagcccgaga atatcgttat cgagatggcc cgagagaacc
     5821 aaactaccca gaagggacag aagaacagta gggaaaggat gaagaggatt gaagagggta
     5881 taaaagaact ggggtcccaa atccttaagg aacacccagt tgaaaacacc cagcttcaga
     5941 atgagaagct ctacctgtac tacctgcaga acggcaggga catgtacgtg gatcaggaac
     6001 tggacatcaa tcggctctcc gactacgacg tggatcatat cgtgccccag tcttttctca
     6061 aagatgattc tattgataat aaagtgttga caagatccga taaaaataga gggaagagtg
     6121 ataacgtccc ctcagaagaa gttgtcaaga aaatgaaaaa ttattggcgg cagctgctga
     6181 acgccaaact gatcacacaa cggaagttcg ataatctgac taaggctgaa cgaggtggcc
     6241 tgtctgagtt ggataaagcc ggcttcatca aaaggcagct tgttgagaca cgccagatca
     6301 ccaagcacgt ggcccaaatt ctcgattcac gcatgaacac caagtacgat gaaaatgaca
     6361 aactgattcg agaggtgaaa gttattactc tgaagtctaa gctggtctca gatttcagaa
     6421 aggactttca gttttataag gtgagagaga tcaacaatta ccaccatgcg catgatgcct
     6481 acctgaatgc agtggtaggc actgcactta tcaaaaaata tcccaagctt gaatctgaat
     6541 ttgtttacgg agactataaa gtgtacgatg ttaggaaaat gatcgcaaag tctgagcagg
     6601 aaataggcaa ggccaccgct aagtacttct tttacagcaa tattatgaat tttttcaaga
     6661 ccgagattac actggccaat ggagagattc ggaagcgacc acttatcgaa acaaacggag
     6721 aaacaggaga aatcgtgtgg gacaagggta gggatttcgc gacagtccgg aaggtcctgt
     6781 ccatgccgca ggtgaacatc gttaaaaaga ccgaagtaca gaccggaggc ttctccaagg
     6841 aaagtatcct cccgaaaagg aacagcgaca agctgatcgc acgcaaaaaa gattgggacc
     6901 ccaagaaata cggcggattc gattctccta cagtcgctta cagtgtactg gttgtggcca
     6961 aagtggagaa agggaagtct aaaaaactca aaagcgtcaa ggaactgctg ggcatcacaa
     7021 tcatggagcg atcaagcttc gaaaaaaacc ccatcgactt tctcgaagcg aaaggatata
     7081 aagaggtcaa aaaagacctc atcattaagc ttcccaagta ctctctcttt gagcttgaaa
     7141 acggccggaa acgaatgctc gctagtgcgg gcgagctgca gaaaggtaac gagctggcac
     7201 tgccctctaa atacgttaat ttcttgtatc tggccagcca ctatgaaaag ctcaaagggt
     7261 ctcccgaaga taatgagcag aagcagctgt tcgtggaaca acacaaacac taccttgatg
     7321 agatcatcga gcaaataagc gagttctcca aaagagtgat cctcgccgac gctaacctcg
     7381 ataaggtgct ttctgcttac aataagcaca gggataagcc catcagggag caggcagaaa
     7441 acattatcca cttgtttact ctgaccaact tgggcgcgcc tgcagccttc aagtacttcg
     7501 acaccaccat agacagaaag cggtacacct ctacaaagga ggtcctggac gccacactga
     7561 ttcatcagtc aattacgggg ctctatgaaa caagaatcga cctctctcag ctcggtggag
     7621 acggaacaag aaagagaaga aaggtgtgac ggatcctcga atatgaagat gaagatgaaa
     7681 tatttggtgt gtcaaataaa aagcttgtgt gcttaagttt gtgttttttt cttggcttgt
     7741 tgtgttatga atttgtggct ttttctaata tcaaatgaat gtaagatctc attataatga
     7801 ataaacaaat gtttctataa tccattgtga atgttttgtt ggatctcttc tgcagcatat
     7861 aactactgta tgtgctatgg tatggactat ggaatatgat taaagataag gtttaaacta
     7921 tcagtgtttg acaggatata ttggcgggta aacctaagag aaaagagcgt ttattagaat
     7981 aatcggatat ttaaaagggc gtgaaaaggt ttatccgttc gtccatttgt atgtgcatgc
     8041 caaccacagg agatccaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     8101 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     8161 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     8221 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     8281 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     8341 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     8401 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     8461 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     8521 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     8581 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     8641 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     8701 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     8761 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     8821 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     8881 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagga
     8941 tctcagtaaa gcgctggctg ctgaaccccc agccggaact gaccccacaa ggccctagcg
     9001 tttgcaatgc accaggtcat cattgaccca ggcgtgttcc accaggccgc tgcctcgcaa
     9061 ctcttcgcag gcttcgccga cctgctcgcg ccacttcttc acgcgggtgg aatccgatcc
     9121 gcacatgagg cggaaggttt ccagcttgag cgggtacggc tcccggtgcg agctgaaata
     9181 gtcgaacatc cgtcgggccg tcggcgacag cttgcggtac ttctcccata tgaatttcgt
     9241 gtagtggtcg ccagcaaaca gcacgacgat ttcctcgtcg atcaggacct ggcaacggga
     9301 cgttttcttg ccacggtcca ggacgcggaa gcggtgcagc agcgacaccg attccaggtg
     9361 cccaacgcgg tcggacgtga agcccatcgc cgtcgcctgt aggcgcgaca ggcattcctc
     9421 ggccttcgtg taataccggc cattgatcga ccagcccagg tcctggcaaa gctcgtagaa
     9481 cgtgaaggtg atcggctcgc cgataggggt gcgcttcgcg tactccaaca cctgctgcca
     9541 caccagttcg tcatcgtcgg cccgcagctc gacgccggtg taggtgatct tcacgtcctt
     9601 gttgacgtgg aaaatgacct tgttttgcag cgcctcgcgc gggattttct tgttgcgcgt
     9661 ggtgaacagg gcagagcggg ccgtgtcgtt tggcatcgct cgcatcgtgt ccggccacgg
     9721 cgcaatatcg aacaaggaaa gctgcatttc cttgatctgc tgcttcgtgt gtttcagcaa
     9781 cgcggcttgc ttggcctcgc tgacctgttt tgccaggtcc tcgccggcgg tttttcgctt
     9841 cttggtcgtc atagttcctc gcgtgtcgat ggtcatcgac ttcgccaaac ctgccgcctc
     9901 ctgttcgaga cgacgcgaac gctccacggc ggccgatggc gcgggcaggg cagggggagc
     9961 cagttgcacg ctgtcgcgct cgatcttggc cgtagcttgc tgggccatcg agccgacgga
    10021 ctggaaggtt tcgcggggcg cacgcatgac ggtgcggctt gcgatggttt cggcatcctc
    10081 ggcggaaaac cccgcgtcga tcagttcttg cctgtatgcc ttccggtcaa acgtccgatt
    10141 cattcaccct ccttgcggga ttgccccgac tcacgccggg gcaatgtgcc cttattcctg
    10201 atttgatccg cctggtgcct tggtgtccag ataatccacc ttatcggcaa tgaagtcggt
    10261 cccgtagacc gtctggccgt ccttctcgta cttggtattc cgaatcttgc cctgcacgaa
    10321 taccagcgac cccttgccca aatacttgcc gtgggcctcg gcctgagagc caaaacactt
    10381 gatgcggaag aagtcggtgc gctcctgctt gtcgccagca tcgttgcgcc acatctaggt
    10441 actaaaacaa ttcatccagt aaaatataat attttatttt ctcccaatca ggcttgatcc
    10501 ccagtaagtc aaaaaatagc tcgacatact gttcttcccc aatatcctcc ctgatcgacc
    10561 ggacgcagaa ggcaatgtca taccacttgt ccgccctgcc gcttctccca agatcaataa
    10621 agccacttac tttgccatct ttcacaaaga tgttgctgtc tcccaggtcg ccgtgggaaa
    10681 agacaagttc ctcttcgggc ttttccgtct ttaaaaaatc atacagctcg cgcggatctt
    10741 taaatggggt gtcttcttcc cagttttcgc aatccacatc ggccagatcg ttattcagta
    10801 agtaatccaa ttcggctaag cggctgtcta agctattcgt atagggacaa tccgatatgt
    10861 cgatggagtg aaagagcctg atgcactccg catacagctc gataatcttt tcagggcttt
    10921 gttcatcttc atactcttcc gagcaaagga cgccatcggc ctcactcatg agcagattgc
    10981 tccagccatc atgccgttca aggtgcagga cctttggaac aggcagcttt ccttccagcc
    11041 atagcatcat gtccttttcc cgttccacat cataggtggt ccctttatac cggctgtccg
    11101 tcatttttaa atataggttt tcattttctc ccaccagctt atatacctta gcaggagaca
    11161 ttccttccgt atcttttacg cagcggtatt tttcgatcag ttttttcaat tccggtgata
    11221 ttctcatttt agccatttat tatttccttc ctcttttcta cagtatttaa agatacccca
    11281 agaagctaat tataacaaga cgaactccaa ttcactgttc cttgcattct aaaactttaa
    11341 ataccagaaa acagcttttt caaagttgtt ttcaaagttg gcgtataaca tagtatcgac
    11401 ggagccgatt ttgaaaccac aattatgggt gatgctgcca acgcgagagc gggccgggag
    11461 ggttcgagaa gggggggcac cccccttcgg cgtgcgcggt cacgcgcaca gggcgcagcc
    11521 ctggttaaaa acaaggttta taaatattgg tttaaaagca ggttaaaaga caggttagcg
    11581 gtggccgaaa aacgggcgga aacccttgca aatgctggat tttctgcctg tggacagccc
    11641 ctcaaatgtc aataggtgcg cccctcatct gtcagcactc tgcccctcaa gtgtcaagga
    11701 tcgcgcccct catctgtcag tagtcgcgcc cctcaagtgt caataccgca gggcacttat
    11761 ccccaggctt gtccacatca tctgtgggaa actcgcgtaa aatcaggcgt tttcgccgat
    11821 ttgcgaggct ggccagctcc acgtcgccgg ccgaaatcga gcctgcccct catctgtcaa
    11881 cgccgcgccg ggtgagtcgg cccctcaagt gtcaacgtcc gcccctcatc tgtcagtgag
    11941 ggccaagttt tccgcgaggt atccacaacg ccggcggctg cggtgtctcg cacacggctt
    12001 cgacggcgtt tctggcgcgt ttgcagggcc atagacggcc gccagcccag cggcgagggc
    12061 aaccagcccg gtgagcgtct ctagtggact gatgggctgc ctgtatcgag tggtgatttt
    12121 gtgccgagct gccggtcggg gagctgttgg ctggctggtg gcaggatata ttgtgg
//
