LOCUS       sequence-382808-       12615 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR, Cas9 driven by double 35S, nptII for plant selection, 
            knockout: CEN12, RR, and Fuzzy3MYB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12615)
  AUTHORS   Ortega MA, Zhou R, Chen MSS, Bewg WP, Simon B, Tsai CJ
  TITLE     In vitro floral development in poplar: insights into seed trichome 
            regulation and trimonoecy.
  JOURNAL   New Phytol. 2022 Nov 16. doi: 10.1111/nph.18624.
  PUBMED    36385612
REFERENCE   2  (bases 1 to 12615)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "New Phytol.
            2022 Nov 16. doi: 10.1111/nph.18624."
COMMENT     Sequence Label: pORE303N_CEN12RRFuzzy3MYB
FEATURES             Location/Qualifiers
     source          1..12615
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    86..110
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(625..1419)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(736..755)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     1346..1365
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        3073..3147
                     /label=AtU6 promoter
                     /note="consensus promoter from the three Arabidopsis U6 
                     snRNA genes (Waibel and Filipowicz, 1990; Nekrasov et al., 
                     2013)"
     misc_RNA        3167..3242
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        3339..4015
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     3952..3972
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             4061..8164
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             4061..8164
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             4061..8164
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     terminator      8204..8452
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_feature    8471..8495
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      8709..9297
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9198..9217
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9451..9468
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(9537..10685)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MAQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(10984..11778)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHLERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      join(11990..12615,1..6)
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 agcgtctcta gtggactgat gggctgcctg tatcgagtgg tgattttgtg ccgagctgcc
       61 ggtcggggag ctgttggctg gctggtggca ggatatattg tggtgtaaac aaattgacga
      121 tttaaatggt aggcgcggct taattaagcg cttaattaag cgcttaatta agcgcttaat
      181 aagccgcgcc gtaccccaat agtctcgaca gacacatagc acctaacatt attgcttaaa
      241 tttactattt ttttactata ttatactcaa cccaatgagc ataaagactg taaaatctca
      301 aattcctgag aagcatattt atcgatccca cagacttgat agttccataa tccatacgct
      361 gcagccaaat tgctagtgtg ttgaacattt aacacgtaga gaactagaaa agatataaaa
      421 ctaagattga tatccaaaat agacgagaac aataagcaaa aactcttagt tttgaaataa
      481 atcaacaatc ccgagggttg tcacatacat caaaaacgaa aatccatata gcaaaaaaaa
      541 ctctaaatta ccgttcgaca aaaagagaaa actgatagga catttgctaa acattaaaat
      601 caaaatttgg atcaagccga attctcagaa gaactcgtca agaaggcgat agaaggcgat
      661 gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc
      721 gccaagctct tcagcaatat cacgggtagc caacgctatg tcctgatagc ggtccgccac
      781 acccagccgg ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg
      841 caagcaggca tcgccatggg tcacgacgag atcatcgccg tcgggcatgc gcgccttgag
      901 cctggcgaac agttcggctg gcgcgagccc ctgatgctct tcgtccagat catcctgatc
      961 gacaagaccg gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc
     1021 gaatgggcag gtagccggat caagcgtatg cagccgccgc attgcatcag ccatgatgga
     1081 tactttctcg gcaggagcaa ggtgagatga caggagatcc tgccccggca cttcgcccaa
     1141 tagcagccag tcccttcccg cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc
     1201 cgtcgtggcc agccacgata gccgcgctgc ctcgtcctgc agttcattca gggcaccgga
     1261 caggtcggtc ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga acacggcggc
     1321 atcagagcag ccgattgtct gttgtgccca gtcatagccg aatagcctct ccacccaagc
     1381 ggccggagaa cctgcgtgca atccatcttg ttcaatcatc tcgagacgta ccgcgcgatg
     1441 cattcgaaga tcgatccttc gcctggagga gagaaatcag tggcgctgcg gcttttaggg
     1501 tttctttgtt gatggaatga gagtgtaagc tctgccagtg ccactttatt agggttttac
     1561 aagccctttt cttcgtaatt gggcctgaca ttttgtgcca cttgggcctt tagagatgaa
     1621 aatgtatatt gggcttaagt tgacttgaag gataaattag tttaggatat tacgtttttt
     1681 atgagaattg gtgtgtcgga tacatgtata tgatgcattc aaatatatgt attctagata
     1741 catttaagtt tagatacaat ctaaaatgtg tctttaatta caggactgta actaaaatac
     1801 ttaatgtaag aagaatatta ctcctttaat agcttttgag tatatctagt ctaacatctt
     1861 ttaaaaaagt ctaatttctt tcatttattt ttcgagcaat agcaaagtgc ataattattt
     1921 ttttcttcta gaaattcaga tttgtttctc taaattttga gattcttttc tcaattttgt
     1981 atgtctagag aacaatgtgt atttttcact ctagttggtt gttactttgt tgaatgttct
     2041 gataaaagta tattgttatt tctgaagtag atataaacct tcatttggaa attatacata
     2101 aatcaaaatc gttaattatc tagatcaaga tatatgccct tttcctaatg tatttgatac
     2161 atgcacctaa tttcactaga tgtatctttt ctatttttta aattatgaat agttaatttt
     2221 ttccatatgt gtatttgata catacttcat gactttaaaa aattaattat ataccagata
     2281 tatgtattta aaatttgtta tgtatttaaa gtatgtatat gattattcga tattaatctc
     2341 ttcgatgaac ctaggatgcc tatcttatat gatcaatgag gcatttaatt gggtgcatat
     2401 gatggtgaaa aaaggtgcag ctcctggctt gggaatgatg actcatgtgg aatttggtct
     2461 taaatttatc acatcctttt gggatgtgat gattgtatca cttgttcatt ttgcaaagac
     2521 aaggtgcact gctacaaact ttggtttaat ctgaaataaa acaaaactca ctgagaggaa
     2581 gatgcatccc agtaggtgaa agtcgagaag gatttgcatg ttactattac acttgctttt
     2641 tagtcccaca tcgtctgaaa cataaaatat ttcagcgttt aaatacttca agcgaaccag
     2701 taggcttgct tgtggttggg agagtgatgt ttaagagcta tgctgaaaag catagcaagt
     2761 ttaaataagg ctagtccgtt aacaacggga aaccgtggca ccgagtcggt gctttttttc
     2821 cacatgggga cacgtggcgc tataagctta tgatttcttt tttcttacga attttgcgtc
     2881 ccacatcggt aagcgagtga agaaataact gctttatata tggctacaaa gcaccattgg
     2941 tcaccatgaa ggagataccg ggtttcagag ctagaaatag caagttgaaa taaggctagt
     3001 ccgttatcac gccgaaaggc gggcaccgag tcggtgcctt tttttgagct gtctgcatgt
     3061 gtgtcagcgg agtgatcaaa agtcccacat cgatcaggtg atatatagca gcttagttta
     3121 tataatgata gagtcgacat agcgattgta ctcaaagcat ggcaaggttt tagagctaga
     3181 aatagcaagt taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt
     3241 gcttttttta ctagttacgg gcccaacatg gtggagcacg acactctcgt ctactccaag
     3301 aatatcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
     3361 atatcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat caaaaggaca
     3421 gtagaaaagg aaggtggcac ctacaaatgc catcattgcg ataaaggaaa ggctatcgtt
     3481 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     3541 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga acatggtgga
     3601 gcacgacact ctcgtctact ccaagaatat caaagataca gtctcagaag accaaagggc
     3661 tattgagact tttcaacaaa gggtaatatc gggaaacctc ctcggattcc attgcccagc
     3721 tatctgtcac ttcatcaaaa ggacagtaga aaaggaaggt ggcacctaca aatgccatca
     3781 ttgcgataaa ggaaaggcta tcgttcaaga tgcctctgcc gacagtggtc ccaaagatgg
     3841 acccccaccc acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca
     3901 agtggattga tgtgatatct ccactgacgt aagggatgac gcacaatccc actatccttc
     3961 gcaagaccct tcctctatat aaggaagttc atttcatttg gagaggacac gctgaaatca
     4021 ccagtctctc tctacaaatc tatctctctc gaccgctagc atggacaaga agtactccat
     4081 tgggctcgat atcggcacaa acagcgtcgg ctgggccgtc attacggacg agtacaaggt
     4141 gccgagcaaa aaattcaaag ttctgggcaa taccgatcgc cacagcataa agaagaacct
     4201 cattggcgcc ctcctgttcg actccgggga gacggccgaa gccacgcggc tcaaaagaac
     4261 agcacggcgc agatataccc gcagaaagaa tcggatctgc tacctgcagg agatctttag
     4321 taatgagatg gctaaggtgg atgactcttt cttccatagg ctggaggagt cctttttggt
     4381 ggaggaggat aaaaagcacg agcgccaccc aatctttggc aatatcgtgg acgaggtggc
     4441 gtaccatgaa aagtacccaa ccatatatca tctgaggaag aagcttgtag acagtactga
     4501 taaggctgac ttgcggttga tctatctcgc gctggcgcat atgatcaaat ttcggggaca
     4561 cttcctcatc gagggggacc tgaacccaga caacagcgat gtcgacaaac tctttatcca
     4621 actggttcag acttacaatc agcttttcga agagaacccg atcaacgcat ccggagttga
     4681 cgccaaagca atcctgagcg ctaggctgtc caaatcccgg cggctcgaaa acctcatcgc
     4741 acagctccct ggggagaaga agaacggcct gtttggtaat cttatcgccc tgtcactcgg
     4801 gctgaccccc aactttaaat ctaacttcga cctggccgaa gatgccaagc ttcaactgag
     4861 caaagacacc tacgatgatg atctcgacaa tctgctggcc cagatcggcg accagtacgc
     4921 agaccttttt ttggcggcaa agaacctgtc agacgccatt ctgctgagtg atattctgcg
     4981 agtgaacacg gagatcacca aagctccgct gagcgctagt atgatcaagc gctatgatga
     5041 gcaccaccaa gacttgactt tgctgaaggc ccttgtcaga cagcaactgc ctgagaagta
     5101 caaggaaatt ttcttcgatc agtctaaaaa tggctacgcc ggatacattg acggcggagc
     5161 aagccaggag gaattttaca aatttattaa gcccatcttg gaaaaaatgg acggcaccga
     5221 ggagctgctg gtaaagctta acagagaaga tctgttgcgc aaacagcgca ctttcgacaa
     5281 tggaagcatc ccccaccaga ttcacctggg cgaactgcac gctatcctca ggcggcaaga
     5341 ggatttctac ccctttttga aagataacag ggaaaagatt gagaaaatcc tcacatttcg
     5401 gataccctac tatgtaggcc ccctcgcccg gggaaattcc agattcgcgt ggatgactcg
     5461 caaatcagaa gagaccatca ctccctggaa cttcgaggaa gtcgtggata agggggcctc
     5521 tgcccagtcc ttcatcgaaa ggatgactaa ctttgataaa aatctgccta acgaaaaggt
     5581 gcttcctaaa cactctctgc tgtacgagta cttcacagtt tataacgagc tgaccaaggt
     5641 caaatacgtc acagaaggga tgagaaagcc agcattcctg tctggagagc agaagaaagc
     5701 tatcgtggac ctcctcttca agacgaaccg gaaagttacc gtgaaacagc tcaaagaaga
     5761 ctatttcaaa aagattgaat gtttcgactc tgttgaaatc agcggagtgg aggatcgctt
     5821 caacgcatcc ctgggaacgt atcacgatct cctgaaaatc attaaagaca aggacttcct
     5881 ggacaatgag gagaacgagg acattcttga ggacattgtc ctcaccctta cgttgtttga
     5941 agatagggag atgattgaag aacgcttgaa aacttacgct catctcttcg acgacaaagt
     6001 catgaaacag ctcaagaggc gccgatatac aggatggggg cggctgtcaa gaaaactgat
     6061 caatggcatc cgagacaagc agagtggaaa gacaatcctg gattttctta agtccgatgg
     6121 atttgccaac cggaacttca tgcagttgat ccatgatgac tctctcacct ttaaggagga
     6181 catccagaaa gcacaagttt ctggccaggg ggacagtctt cacgagcaca tcgctaatct
     6241 tgcaggtagc ccagctatca aaaagggaat actgcagacc gttaaggtcg tggatgaact
     6301 cgtcaaagta atgggaaggc ataagcccga gaatatcgtt atcgagatgg cccgagagaa
     6361 ccaaactacc cagaagggac agaagaacag tagggaaagg atgaagagga ttgaagaggg
     6421 tataaaagaa ctggggtccc aaatccttaa ggaacaccca gttgaaaaca cccagcttca
     6481 gaatgagaag ctctacctgt actacctgca gaacggcagg gacatgtacg tggatcagga
     6541 actggacatc aatcggctct ccgactacga cgtggatcat atcgtgcccc agtcttttct
     6601 caaagatgat tctattgata ataaagtgtt gacaagatcc gataaaaata gagggaagag
     6661 tgataacgtc ccctcagaag aagttgtcaa gaaaatgaaa aattattggc ggcagctgct
     6721 gaacgccaaa ctgatcacac aacggaagtt cgataatctg actaaggctg aacgaggtgg
     6781 cctgtctgag ttggataaag ccggcttcat caaaaggcag cttgttgaga cacgccagat
     6841 caccaagcac gtggcccaaa ttctcgattc acgcatgaac accaagtacg atgaaaatga
     6901 caaactgatt cgagaggtga aagttattac tctgaagtct aagctggtct cagatttcag
     6961 aaaggacttt cagttttata aggtgagaga gatcaacaat taccaccatg cgcatgatgc
     7021 ctacctgaat gcagtggtag gcactgcact tatcaaaaaa tatcccaagc ttgaatctga
     7081 atttgtttac ggagactata aagtgtacga tgttaggaaa atgatcgcaa agtctgagca
     7141 ggaaataggc aaggccaccg ctaagtactt cttttacagc aatattatga attttttcaa
     7201 gaccgagatt acactggcca atggagagat tcggaagcga ccacttatcg aaacaaacgg
     7261 agaaacagga gaaatcgtgt gggacaaggg tagggatttc gcgacagtcc ggaaggtcct
     7321 gtccatgccg caggtgaaca tcgttaaaaa gaccgaagta cagaccggag gcttctccaa
     7381 ggaaagtatc ctcccgaaaa ggaacagcga caagctgatc gcacgcaaaa aagattggga
     7441 ccccaagaaa tacggcggat tcgattctcc tacagtcgct tacagtgtac tggttgtggc
     7501 caaagtggag aaagggaagt ctaaaaaact caaaagcgtc aaggaactgc tgggcatcac
     7561 aatcatggag cgatcaagct tcgaaaaaaa ccccatcgac tttctcgaag cgaaaggata
     7621 taaagaggtc aaaaaagacc tcatcattaa gcttcccaag tactctctct ttgagcttga
     7681 aaacggccgg aaacgaatgc tcgctagtgc gggcgagctg cagaaaggta acgagctggc
     7741 actgccctct aaatacgtta atttcttgta tctggccagc cactatgaaa agctcaaagg
     7801 gtctcccgaa gataatgagc agaagcagct gttcgtggaa caacacaaac actaccttga
     7861 tgagatcatc gagcaaataa gcgagttctc caaaagagtg atcctcgccg acgctaacct
     7921 cgataaggtg ctttctgctt acaataagca cagggataag cccatcaggg agcaggcaga
     7981 aaacattatc cacttgttta ctctgaccaa cttgggcgcg cctgcagcct tcaagtactt
     8041 cgacaccacc atagacagaa agcggtacac ctctacaaag gaggtcctgg acgccacact
     8101 gattcatcag tcaattacgg ggctctatga aacaagaatc gacctctctc agctcggtgg
     8161 agacggaaca agaaagagaa gaaaggtgtg acggatcctc gaatatgaag atgaagatga
     8221 aatatttggt gtgtcaaata aaaagcttgt gtgcttaagt ttgtgttttt ttcttggctt
     8281 gttgtgttat gaatttgtgg ctttttctaa tatcaaatga atgtaagatc tcattataat
     8341 gaataaacaa atgtttctat aatccattgt gaatgttttg ttggatctct tctgcagcat
     8401 ataactactg tatgtgctat ggtatggact atggaatatg attaaagata aggtttaaac
     8461 tatcagtgtt tgacaggata tattggcggg taaacctaag agaaaagagc gtttattaga
     8521 ataatcggat atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt gtatgtgcat
     8581 gccaaccaca ggagatccaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     8641 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     8701 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     8761 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     8821 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     8881 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     8941 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     9001 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     9061 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     9121 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     9181 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     9241 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     9301 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     9361 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     9421 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     9481 gatctcagta aagcgctggc tgctgaaccc ccagccggaa ctgaccccac aaggccctag
     9541 cgtttgcaat gcaccaggtc atcattgacc caggcgtgtt ccaccaggcc gctgcctcgc
     9601 aactcttcgc aggcttcgcc gacctgctcg cgccacttct tcacgcgggt ggaatccgat
     9661 ccgcacatga ggcggaaggt ttccagcttg agcgggtacg gctcccggtg cgagctgaaa
     9721 tagtcgaaca tccgtcgggc cgtcggcgac agcttgcggt acttctccca tatgaatttc
     9781 gtgtagtggt cgccagcaaa cagcacgacg atttcctcgt cgatcaggac ctggcaacgg
     9841 gacgttttct tgccacggtc caggacgcgg aagcggtgca gcagcgacac cgattccagg
     9901 tgcccaacgc ggtcggacgt gaagcccatc gccgtcgcct gtaggcgcga caggcattcc
     9961 tcggccttcg tgtaataccg gccattgatc gaccagccca ggtcctggca aagctcgtag
    10021 aacgtgaagg tgatcggctc gccgataggg gtgcgcttcg cgtactccaa cacctgctgc
    10081 cacaccagtt cgtcatcgtc ggcccgcagc tcgacgccgg tgtaggtgat cttcacgtcc
    10141 ttgttgacgt ggaaaatgac cttgttttgc agcgcctcgc gcgggatttt cttgttgcgc
    10201 gtggtgaaca gggcagagcg ggccgtgtcg tttggcatcg ctcgcatcgt gtccggccac
    10261 ggcgcaatat cgaacaagga aagctgcatt tccttgatct gctgcttcgt gtgtttcagc
    10321 aacgcggctt gcttggcctc gctgacctgt tttgccaggt cctcgccggc ggtttttcgc
    10381 ttcttggtcg tcatagttcc tcgcgtgtcg atggtcatcg acttcgccaa acctgccgcc
    10441 tcctgttcga gacgacgcga acgctccacg gcggccgatg gcgcgggcag ggcaggggga
    10501 gccagttgca cgctgtcgcg ctcgatcttg gccgtagctt gctgggccat cgagccgacg
    10561 gactggaagg tttcgcgggg cgcacgcatg acggtgcggc ttgcgatggt ttcggcatcc
    10621 tcggcggaaa accccgcgtc gatcagttct tgcctgtatg ccttccggtc aaacgtccga
    10681 ttcattcacc ctccttgcgg gattgccccg actcacgccg gggcaatgtg cccttattcc
    10741 tgatttgatc cgcctggtgc cttggtgtcc agataatcca ccttatcggc aatgaagtcg
    10801 gtcccgtaga ccgtctggcc gtccttctcg tacttggtat tccgaatctt gccctgcacg
    10861 aataccagcg accccttgcc caaatacttg ccgtgggcct cggcctgaga gccaaaacac
    10921 ttgatgcgga agaagtcggt gcgctcctgc ttgtcgccag catcgttgcg ccacatctag
    10981 gtactaaaac aattcatcca gtaaaatata atattttatt ttctcccaat caggcttgat
    11041 ccccagtaag tcaaaaaata gctcgacata ctgttcttcc ccaatatcct ccctgatcga
    11101 ccggacgcag aaggcaatgt cataccactt gtccgccctg ccgcttctcc caagatcaat
    11161 aaagccactt actttgccat ctttcacaaa gatgttgctg tctcccaggt cgccgtggga
    11221 aaagacaagt tcctcttcgg gcttttccgt ctttaaaaaa tcatacagct cgcgcggatc
    11281 tttaaatggg gtgtcttctt cccagttttc gcaatccaca tcggccagat cgttattcag
    11341 taagtaatcc aattcggcta agcggctgtc taagctattc gtatagggac aatccgatat
    11401 gtcgatggag tgaaagagcc tgatgcactc cgcatacagc tcgataatct tttcagggct
    11461 ttgttcatct tcatactctt ccgagcaaag gacgccatcg gcctcactca tgagcagatt
    11521 gctccagcca tcatgccgtt caaggtgcag gacctttgga acaggcagct ttccttccag
    11581 ccatagcatc atgtcctttt cccgttccac atcataggtg gtccctttat accggctgtc
    11641 cgtcattttt aaatataggt tttcattttc tcccaccagc ttatatacct tagcaggaga
    11701 cattccttcc gtatctttta cgcagcggta tttttcgatc agttttttca attccggtga
    11761 tattctcatt ttagccattt attatttcct tcctcttttc tacagtattt aaagataccc
    11821 caagaagcta attataacaa gacgaactcc aattcactgt tccttgcatt ctaaaacttt
    11881 aaataccaga aaacagcttt ttcaaagttg ttttcaaagt tggcgtataa catagtatcg
    11941 acggagccga ttttgaaacc acaattatgg gtgatgctgc caacgcgaga gcgggccggg
    12001 agggttcgag aagggggggc accccccttc ggcgtgcgcg gtcacgcgca cagggcgcag
    12061 ccctggttaa aaacaaggtt tataaatatt ggtttaaaag caggttaaaa gacaggttag
    12121 cggtggccga aaaacgggcg gaaacccttg caaatgctgg attttctgcc tgtggacagc
    12181 ccctcaaatg tcaataggtg cgcccctcat ctgtcagcac tctgcccctc aagtgtcaag
    12241 gatcgcgccc ctcatctgtc agtagtcgcg cccctcaagt gtcaataccg cagggcactt
    12301 atccccaggc ttgtccacat catctgtggg aaactcgcgt aaaatcaggc gttttcgccg
    12361 atttgcgagg ctggccagct ccacgtcgcc ggccgaaatc gagcctgccc ctcatctgtc
    12421 aacgccgcgc cgggtgagtc ggcccctcaa gtgtcaacgt ccgcccctca tctgtcagtg
    12481 agggccaagt tttccgcgag gtatccacaa cgccggcggc tgcggtgtct cgcacacggc
    12541 ttcgacggcg tttctggcgc gtttgcaggg ccatagacgg ccgccagccc agcggcgagg
    12601 gcaaccagcc cggtg
//
