LOCUS       sequence-382814-       13544 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nimble cloning-compatible vector for plant ectopic expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13544)
  AUTHORS   Yan P, Tuo D, Shen W, Deng H, Zhou P, Gao X
  TITLE     A Nimble Cloning-compatible vector system for high-throughput gene 
            functional analysis in plants.
  JOURNAL   Plant Commun. 2023 Mar 13;4(2):100471. doi: 
            10.1016/j.xplc.2022.100471. Epub 2022 Nov 9.
  PUBMED    36352791
REFERENCE   2  (bases 1 to 13544)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Commun."; date: "2023-03-13"; volume: "4(2):100471. doi"; pages: " 
            10.1016/j.xplc.2022.100471. Epub 2022 Nov 9"
COMMENT     Sequence Label: pNC-121-35S
FEATURES             Location/Qualifiers
     source          1..13544
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(128..499)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            532..641
                     /note="incP origin of transfer"
     primer_bind     864..883
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1117..1134
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1322..1972)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2437..2461
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2585..2764
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        2617..2800
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             2821..3615
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2875..2894)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3485..3504
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      4005..4257
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    4806..4827
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4842..4847,4848..4865,4866..4872)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4880..4896
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4885..4907
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4904..4920
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4904..4920
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        5444..5789
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     5732..5752
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     promoter        5985..6087
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             6118..6423
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     6302..6321
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     terminator      6496..6748
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(6757..6774)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6757..6773)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6766..6788)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(6913..6932)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7123..7144
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    7391..7415
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(9011..10159)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(10458..11252)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  11444..12211
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      12904..13535
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 ctcggtcttg ccttgctcgt cggtgatgta cttcaccagc tccgcgaagt cgctcttctt
       61 gatggagcgc atggggacgt gcttggcaat cacgcgcacc ccccggccgt tttagcggct
      121 aaaaaagtca tggctctgcc ctcgggcgga ccacgcccat catgaccttg ccaagctcgt
      181 cctgcttctc ttcgatcttc gccagcaggg cgaggatcgt ggcatcaccg aaccgcgccg
      241 tgcgcgggtc gtcggtgagc cagagtttca gcaggccgcc caggcggccc aggtcgccat
      301 tgatgcgggc cagctcgcgg acgtgctcat agtccacgac gcccgtgatt ttgtagccct
      361 ggccgacggc cagcaggtag gccgacaggc tcatgccggc cgccgccgcc ttttcctcaa
      421 tcgctcttcg ttcgtctgga aggcagtaca ccttgatagg tgggctgccc ttcctggttg
      481 gcttggtttc atcagccatc cgcttgccct catctgttac gccggcggta gccggccagc
      541 ctcgcagagc aggattcccg ttgagcaccg ccaggtgcga ataagggaca gtgaagaagg
      601 aacacccgct cgcgggtggg cctacttcac ctatcctgcc cggctgacgc cgttggatac
      661 accaaggaaa gtctacacga accctttggc aaaatcctgt atatcgtgcg aaaaaggatg
      721 gatataccga aaaaatcgct ataatgaccc cgaagcaggg ttatgcagcg gaaaagcgcc
      781 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
      841 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
      901 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
      961 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     1021 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     1081 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     1141 gaagagcgcc agaaggccgc cagagaggcc gagcgcggcc gtgaggcttg gacgctaggg
     1201 cagggcatga aaaagcccgt agcgggctgc tacgggcgtc tgacgcggtg gaaaggggga
     1261 ggggatgttg tctacatggc tctgctgtag tgagtgggtt gcgctccggc agcggtcctg
     1321 atcaatcgtc accctttctc ggtccttcaa cgttcctgac aacgagcctc cttttcgcca
     1381 atccatcgac aatcaccgcg agtccctgct cgaacgctgc gtccggaccg gcttcgtcga
     1441 aggcgtctat cgcggcccgc aacagcggcg agagcggagc ctgttcaacg gtgccgccgc
     1501 gctcgccggc atcgctgtcg ccggcctgct cctcaagcac ggccccaaca gtgaagtagc
     1561 tgattgtcat cagcgcattg acggcgtccc cggccgaaaa acccgcctcg cagaggaagc
     1621 gaagctgcgc gtcggccgtt tccatctgcg gtgcgcccgg tcgcgtgccg gcatggatgc
     1681 gcgcgccatc gcggtaggcg agcagcgcct gcctgaagct gcgggcattc ccgatcagaa
     1741 atgagcgcca gtcgtcgtcg gctctcggca ccgaatgcgt atgattctcc gccagcatgg
     1801 cttcggccag tgcgtcgagc agcgcccgct tgttcctgaa gtgccagtaa agcgccggct
     1861 gctgaacccc caaccgttcc gccagtttgc gtgtcgtcag accgtctacg ccgacctcgt
     1921 tcaacaggtc cagggcggca cggatcactg tattcggctg caactttgtc atgcttgaca
     1981 ctttatcact gataaacata atatgtccac caacttatca gtgataaaga atccgcgcgt
     2041 tcaatcggac cagcggaggc tggtccggag gccagacgtg aaacccaaca tacccctgat
     2101 cgtaattctg agcactgtcg cgctcgacgc tgtcggcatc ggcctgatta tgccggtgct
     2161 gccgggcctc ctgcgcgatc tggttcactc gaacgacgtc accgcccact atggcattct
     2221 gctggcgctg tatgcgttgg tgcaatttgc ctgcgcacct gtgctgggcg cgctgtcgga
     2281 tcgtttcggg cggcggccaa tcttgctcgt ctcgctggcc ggcgccagat ctggggaacc
     2341 ctgtggttgg catgcacata caaatggacg aacggataaa ccttttcacg cccttttaaa
     2401 tatccgatta ttctaataaa cgctcttttc tcttaggttt acccgccaat atatcctgtc
     2461 aaacactgat agtttaaact gaaggcggga aacgacaatc tgatcatgag cggagaatta
     2521 agggagtcac gttatgaccc ccgccgatga cgcgggacaa gccgttttac gtttggaact
     2581 gacagaaccg caacgttgaa ggagccactc agccgcgggt ttctggagtt taatgagcta
     2641 agcacatacg tcagaaacca ttattgcgcg ttcaaaagtc gcctaaggtc actatcagct
     2701 agcaaatatt tcttgtcaaa aatgctccac tgacgttcca taaattcccc tcggtatcca
     2761 attagagtct catattcact ctcaatccaa ataatctgca ccggatctgg atcgtttcgc
     2821 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
     2881 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
     2941 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
     3001 caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
     3061 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
     3121 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
     3181 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
     3241 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
     3301 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac
     3361 ggcgatgatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
     3421 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
     3481 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
     3541 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
     3601 gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc
     3661 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
     3721 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
     3781 cccacgggat ctctgcggaa caggcggtcg aaggtgccga tatcattacg acagcaacgg
     3841 ccgacaagca caacgccacg atcctgagcg acaatatgat cgggcccggc gtccacatca
     3901 acggcgtcgg cggcgactgc ccaggcaaga ccgagatgca ccgcgatatc ttgctgcgtt
     3961 cggatatttt cgtggagttc ccgccacaga cccggatgat ccccgatcgt tcaaacattt
     4021 ggcaataaag tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt atcatataat
     4081 ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg ttatttatga
     4141 gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata gaaaacaaaa
     4201 tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc atctatgtta ctagatcggg
     4261 cctcctgtca atgctggcgg cggctctggt ggtggttctg gtggcggctc tgagggtggt
     4321 ggctctgagg gtggcggttc tgagggtggc ggctctgagg gaggcggttc cggtggtggc
     4381 tctggttccg gtgattttga ttatgaaaag atggcaaacg ctaataaggg ggctatgacc
     4441 gaaaatgccg atgaaaacgc gctacagtct gacgctaaag gcaaacttga ttctgtcgct
     4501 actgattacg gtgctgctat cgatggtttc attggtgacg tttccggcct tgctaatggt
     4561 aatggtgcta ctggtgattt tgctggctct aattcccaaa tggctcaagt cggtgacggt
     4621 gataattcac ctttaatgaa taatttccgt caatatttac cttccctccc tcaatcggtt
     4681 gaatgtcgcc cttttgtctt tggcccaata cgcaaaccgc ctctccccgc gcgttggccg
     4741 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     4801 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     4861 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     4921 catgattacg ccaagcttgc atgcctgcag gtccccagat tagccttttc aatttcagaa
     4981 agaatgctaa cccacagatg gttagagagg cttacgcagc aggtctcatc aagacgatct
     5041 acccgagcaa taatctccag gaaatcaaat accttcccaa gaaggttaaa gatgcagtca
     5101 aaagattcag gactaactgc atcaagaaca cagagaaaga tatatttctc aagatcagaa
     5161 gtactattcc agtatggacg attcaaggct tgcttcacaa accaaggcaa gtaatagaga
     5221 ttggagtctc taaaaaggta gttcccactg aatcaaaggc catggagtca aagattcaaa
     5281 tagaggacct aacagaactc gccgtaaaga ctggcgaaca gttcatacag agtctcttac
     5341 gactcaatga caagaagaaa atcttcgtca acatggtgga gcacgacaca cttgtctact
     5401 ccaaaaatat caaagataca gtctcagaag accaaagggc aattgagact tttcaacaaa
     5461 gggtaatatc cggaaacctc ctcggattcc attgcccagc tatctgtcac tttattgtga
     5521 agatagtgga aaaggaaggt ggctcctaca aatgccatca ttgcgataaa ggaaaggcca
     5581 tcgttgaaga tgcctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca
     5641 tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca agtggattga tgtgatatct
     5701 ccactgacgt aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat
     5761 aaggaagttc atttcatttg gagagaacac gggggactct agagagtggt ctctgtccag
     5821 tcctggcctc gtcggccatt ctcgactaag ttggcagcat cacccgacgc actttgcgcc
     5881 gaataaatac ctgtgacgga agatcacttc gcagaataaa taaatcctgg tgtccctgtt
     5941 gataccggga agccctgggc caacttttgg cgaaaatgag acgttgatcg gcacgtaaga
     6001 ggttccaact ttcaccataa tgaaataaga tcactaccgg gcgtattttt tgagttatcg
     6061 agattttcag gagctaagga agctaaactt ttgctgacga gaacagggac tggtgaaatg
     6121 cagtttaagg tttacaccta taaaagagag agccgttatc gtctgtttgt ggatgtacag
     6181 agtgatatta ttgacacgcc tgggcgacgg atggtgatcc ccctggccag tgcacgtctg
     6241 ctgtcagata aagtctcccg tgaactttac ccggtggtgc atatcgggga tgaaagctgg
     6301 cgcatgatga ccaccgatat ggccagtgtg ccggtatccg ttatcgggga agaagtggct
     6361 gatctcagcc accgcgaaaa tgacatcaaa aacgccatta acctgatgtt ctggggaata
     6421 taaatgtcag gctcccttat acacagggcc agtctggcca cttgtggtct gctgagaccg
     6481 agctcgaatt tccccgatcg ttcaaacatt tggcaataaa gtttcttaag attgaatcct
     6541 gttgccggtc ttgcgatgat tatcatataa tttctgttga attacgttaa gcatgtaata
     6601 attaacatgt aatgcatgac gttatttatg agatgggttt ttatgattag agtcccgcaa
     6661 ttatacattt aatacgcgat agaaaacaaa atatagcgcg caaactagga taaattatcg
     6721 cgcgcggtgt catctatgtt actagatcgg gaattcactg gccgtcgttt tacaacgtcg
     6781 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
     6841 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
     6901 gaatggcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     6961 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     7021 tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga
     7081 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     7141 ctggaacaac actcaaccct atctcgggct attcttttga tttataaggg attttgccga
     7201 tttcggaacc accatcaaac aggattttcg cctgctgggg caaaccagcg tggaccgctt
     7261 gctgcaactc tctcagggcc aggcggtgaa gggcaatcag ctgttgcccg tctcactggt
     7321 gaaaagaaaa accaccccag tacattaaaa acgtccgcaa tgtgttatta agttgtctaa
     7381 gcgtcaattt gtttacacca caatatatcc tgccaccagc cagccaacag ctccccgacc
     7441 ggcagctcgg cacaaaatca ccactcgata caggcagccc atcagtccgg gacggcgtca
     7501 gcgggagagc cgttgtaagg cggcagactt tgctcatgtt accgatgcta ttcggaagaa
     7561 cggcaactaa gctgccgggt ttgaaacacg gatgatctcg cggagggtag catgttgatt
     7621 gtaacgatga cagagcgttg ctgcctgtga tcaaatatca tctccctcgc agagatccga
     7681 attatcagcc ttcttattca tttctcgctt aaccgtgaca ggctgtcgat cttgagaact
     7741 atgccgacat aataggaaat cgctggataa agccgctgag gaagctgagt ggcgctattt
     7801 ctttagaagt gaacgttgac gatatcaact cccctatcca ttgctcaccg aatggtacag
     7861 gtcggggacc cgaagttccg actgtcggcc tgatgcatcc ccggctgatc gaccccagat
     7921 ctggggctga gaaagcccag taaggaaaca actgtaggtt cgagtcgcga gatcccccgg
     7981 aaccaaagga agtaggttaa acccgctccg atcaggccga gccacgccag gccgagaaca
     8041 ttggttcctg taggcatcgg gattggcgga tcaaacacta aagctactgg aacgagcaga
     8101 agtcctccgg ccgccagttg ccaggcggta aaggtgagca gaggcacggg aggttgccac
     8161 ttgcgggtca gcacggttcc gaacgccatg gaaaccgccc ccgccaggcc cgctgcgacg
     8221 ccgacaggat ctagcgctgc gtttggtgtc aacaccaaca gcgccacgcc cgcagttccg
     8281 caaatagccc ccaggaccgc catcaatcgt atcgggctac ctagcagagc ggcagagatg
     8341 aacacgacca tcagcggctg cacagcgcct accgtcgccg cgaccccgcc cggcaggcgg
     8401 tagaccgaaa taaacaacaa gctccagaat agcgaaatat taagtgcgcc gaggatgaag
     8461 atgcgcatcc accagattcc cgttggaatc tgtcggacga tcatcacgag caataaaccc
     8521 gccggcaacg cccgcagcag cataccggcg acccctcggc ctcgctgttc gggctccacg
     8581 aaaacgccgg acagatgcgc cttgtgagcg tccttggggc cgtcctcctg tttgaagacc
     8641 gacagcccaa tgatctcgcc gtcgatgtag gcgccgaatg ccacggcatc tcgcaaccgt
     8701 tcagcgaacg cctccatggg ctttttctcc tcgtgctcgt aaacggaccc gaacatctct
     8761 ggagctttct tcagggccga caatcggatc tcgcggaaat cctgcacgtc ggccgctcca
     8821 agccgtcgaa tctgagcctt aatcacaatt gtcaatttta atcctctgtt tatcggcagt
     8881 tcgtagagcg cgccgtgcgt cccgagcgat actgagcgaa gcaagtgcgt cgagcagtgc
     8941 ccgcttgttc ctgaaatgcc agtaaagcgc tggctgctga acccccagcc ggaactgacc
     9001 ccacaaggcc ctagcgtttg caatgcacca ggtcatcatt gacccaggcg tgttccacca
     9061 ggccgctgcc tcgcaactct tcgcaggctt cgccgacctg ctcgcgccac ttcttcacgc
     9121 gggtggaatc cgatccgcac atgaggcgga aggtttccag cttgagcggg tacggctccc
     9181 ggtgcgagct gaaatagtcg aacatccgtc gggccgtcgg cgacagcttg cggtacttct
     9241 cccatatgaa tttcgtgtag tggtcgccag caaacagcac gacgatttcc tcgtcgatca
     9301 ggacctggca acgggacgtt ttcttgccac ggtccaggac gcggaagcgg tgcagcagcg
     9361 acaccgattc caggtgccca acgcggtcgg acgtgaagcc catcgccgtc gcctgtaggc
     9421 gcgacaggca ttcctcggcc ttcgtgtaat accggccatt gatcgaccag cccaggtcct
     9481 ggcaaagctc gtagaacgtg aaggtgatcg gctcgccgat aggggtgcgc ttcgcgtact
     9541 ccaacacctg ctgccacacc agttcgtcat cgtcggcccg cagctcgacg ccggtgtagg
     9601 tgatcttcac gtccttgttg acgtggaaaa tgaccttgtt ttgcagcgcc tcgcgcggga
     9661 ttttcttgtt gcgcgtggtg aacagggcag agcgggccgt gtcgtttggc atcgctcgca
     9721 tcgtgtccgg ccacggcgca atatcgaaca aggaaagctg catttccttg atctgctgct
     9781 tcgtgtgttt cagcaacgcg gcttgcttgg cctcgctgac ctgttttgcc aggtcctcgc
     9841 cggcggtttt tcgcttcttg gtcgtcatag ttcctcgcgt gtcgatggtc atcgacttcg
     9901 ccaaacctgc cgcctcctgt tcgagacgac gcgaacgctc cacggcggcc gatggcgcgg
     9961 gcagggcagg gggagccagt tgcacgctgt cgcgctcgat cttggccgta gcttgctgga
    10021 ccatcgagcc gacggactgg aaggtttcgc ggggcgcacg catgacggtg cggcttgcga
    10081 tggtttcggc atcctcggcg gaaaaccccg cgtcgatcag ttcttgcctg tatgccttcc
    10141 ggtcaaacgt ccgattcatt caccctcctt gcgggattgc cccgactcac gccggggcaa
    10201 tgtgccctta ttcctgattt gacccgcctg gtgccttggt gtccagataa tccaccttat
    10261 cggcaatgaa gtcggtcccg tagaccgtct ggccgtcctt ctcgtacttg gtattccgaa
    10321 tcttgccctg cacgaatacc agcgacccct tgcccaaata cttgccgtgg gcctcggcct
    10381 gagagccaaa acacttgatg cggaagaagt cggtgcgctc ctgcttgtcg ccggcatcgt
    10441 tgcgccacat ctaggtacta aaacaattca tccagtaaaa tataatattt tattttctcc
    10501 caatcaggct tgatccccag taagtcaaaa aatagctcga catactgttc ttccccgata
    10561 tcctccctga tcgaccggac gcagaaggca atgtcatacc acttgtccgc cctgccgctt
    10621 ctcccaagat caataaagcc acttactttg ccatctttca caaagatgtt gctgtctccc
    10681 aggtcgccgt gggaaaagac aagttcctct tcgggctttt ccgtctttaa aaaatcatac
    10741 agctcgcgcg gatctttaaa tggagtgtct tcttcccagt tttcgcaatc cacatcggcc
    10801 agatcgttat tcagtaagta atccaattcg gctaagcggc tgtctaagct attcgtatag
    10861 ggacaatccg atatgtcgat ggagtgaaag agcctgatgc actccgcata cagctcgata
    10921 atcttttcag ggctttgttc atcttcatac tcttccgagc aaaggacgcc atcggcctca
    10981 ctcatgagca gattgctcca gccatcatgc cgttcaaagt gcaggacctt tggaacaggc
    11041 agctttcctt ccagccatag catcatgtcc ttttcccgtt ccacatcata ggtggtccct
    11101 ttataccggc tgtccgtcat ttttaaatat aggttttcat tttctcccac cagcttatat
    11161 accttagcag gagacattcc ttccgtatct tttacgcagc ggtatttttc gatcagtttt
    11221 ttcaattccg gtgatattct cattttagcc atttattatt tccttcctct tttctacagt
    11281 atttaaagat accccaagaa gctaattata acaagacgaa ctccaattca ctgttccttg
    11341 cattctaaaa ccttaaatac cagaaaacag ctttttcaaa gttgttttca aagttggcgt
    11401 ataacatagt atcgacggag ccgattttga aaccacaatt atgggtgatg ctgccaactt
    11461 actgatttag tgtatgatgg tgtttttgag gtgctccagt ggcttctgtt tctatcagct
    11521 gtccctcctg ttcagctact gacggggtgg tgcgtaacgg caaaagcacc gccggacatc
    11581 agcgctatct ctgctctcac tgccgtaaaa catggcaact gcagttcact tacaccgctt
    11641 ctcaacccgg tacgcaccag aaaatcattg atatggccat gaatggcgtt ggatgccggg
    11701 caacagcccg cattatgggc gttggcctca acacgatttt acgtcactta aaaaactcag
    11761 gccgcagtcg gtaacctcgc gcatacagcc gggcagtgac gtcatcgtct gcgcggaaat
    11821 ggacgaacag tggggctatg tcggggctaa atcgcgccag cgctggctgt tttacgcgta
    11881 tgacagtctc cggaagacgg ttgttgcgca cgtattcggt gaacgcacta tggcgacgct
    11941 ggggcgtctt atgagcctgc tgtcaccctt tgacgtggtg atatggatga cggatggctg
    12001 gccgctgtat gaatcccgcc tgaagggaaa gctgcacgta atcagcaagc gatatacgca
    12061 gcgaattgag cggcataacc tgaatctgag gcagcacctg gcacggctgg gacggaagtc
    12121 gctgtcgttc tcaaaatcgg tggagctgca tgacaaagtc atcgggcatt atctgaacat
    12181 aaaacactat caataagttg gagtcattac ccaattatga tagaatttac aagctataag
    12241 gttattgtcc tgggtttcaa gcattagtcc atgcaagttt ttatgctttg cccattctat
    12301 agatatattg ataagcgcgc tgcctatgcc ttgccccctg aaatccttac atacggcgat
    12361 atcttctata taaaagatat attatcttat cagtattgtc aatatattca aggcaatctg
    12421 cctcctcatc ctcttcatcc tcttcgtctt ggtagctttt taaatatggc gcttcataga
    12481 gtaattctgt aaaggtccaa ttctcgtttt catacctcgg tataatctta cctatcacct
    12541 caaatggttc gctgggttta tcgcaccccc gaacacgagc acggcacccg cgaccactat
    12601 gccaagaatg cccaaggtaa aaattgccgg ccccgccatg aagtccgtga atgccccgac
    12661 ggccgaagtg aagggcaggc cgccacccag gccgccgccc tcactgcccg gcacctggtc
    12721 gctgaatgtc gatgccagca cctgcggcac gtcaatgctt ccgggcgtcg cgctcgggct
    12781 gatcgcccat cccgttactg ccccgatccc ggcaatggca aggactgcca gcgctgccat
    12841 ttttggggtg aggccgttcg cggccgaggg gcgcagcccc tggggggatg ggaggcccgc
    12901 gttagcgggc cgggagggtt cgagaagggg gggcaccccc cttcggcgtg cgcggtcacg
    12961 cgcacagggc gcagccctgg ttaaaaacaa ggtttataaa tattggttta aaagcaggtt
    13021 aaaagacagg ttagcggtgg ccgaaaaacg ggcggaaacc cttgcaaatg ctggattttc
    13081 tgcctgtgga cagcccctca aatgtcaata ggtgcgcccc tcatctgtca gcactctgcc
    13141 cctcaagtgt caaggatcgc gcccctcatc tgtcagtagt cgcgcccctc aagtgtcaat
    13201 accgcagggc acttatcccc aggcttgtcc acatcatctg tgggaaactc gcgtaaaatc
    13261 aggcgttttc gccgatttgc gaggctggcc agctccacgt cgccggccga aatcgagcct
    13321 gcccctcatc tgtcaacgcc gcgccgggtg agtcggcccc tcaagtgtca acgtccgccc
    13381 ctcatctgtc agtgagggcc aagttttccg cgaggtatcc acaacgccgg cggccgcggt
    13441 gtctcgcaca cggcttcgac ggcgtttctg gcgcgtttgc agggccatag acggccgcca
    13501 gcccagcggc gagggcaacc agcccggtga gcgtcggaaa ggcg
//
