LOCUS       sequence-382846-        8497 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of CG10077-RA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8497)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 8497)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAc5.1B-EGFP-CG10077-RA_B
FEATURES             Location/Qualifiers
     source          1..8497
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..2510
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             join(2530..2532,2533..2535,2536..3246)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2530..2532,2533..2535,2536..3246)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2575..2596)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2836..2855)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3183..3204
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5748..5789
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5799..5816
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5839..5856)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5889..6023
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5913..5932)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5967..5986
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6624..7212)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6704..6723)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7383..8174,8175..8243))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8006..8025
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8244..8348)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8416..8434
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 catgaatggc atcaactctg aatcaaatct ttgcagatgc acctacttct catttccact
       61 gtcacatcat ttttccagat ctcgctgcct gttatgtggc ccacaaacca agacacgttt
      121 tatggccatt aaagctggct gatcgtcgcc aaacaccaaa tacataatga atatgtacac
      181 attcgagaaa gaagcgatca aagaagcgtc ttcgggcgga gtaggagaat gcggaggaga
      241 aggagaacga gctgatctag tatctctcca caatccaatg ccaactgacc aactggccat
      301 attcggagca atttgaagcc aatttccatc gcctggcgat cgctccattc ttggctatat
      361 gtttttcacc gttacccggg gccattttca aagactcgtc ggcaagataa gattgtgtca
      421 ctcgctgtct ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag
      481 tattttgaag aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac
      541 aaaaacatgt tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt
      601 atgtatatac ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac
      661 tgatgatatt ttaagatgat gccagaccaa aaggcttgaa tttctgcgtc ttttgccgaa
      721 cgcagtgcat gtgcaattgt tgttttttgg aatattcaat tttcggactg tccgctttga
      781 tttcagtttc ttggcttatt caaaaagcaa agtaaagcca aaaaagcgag atggcaatac
      841 caaatgcggc aaaacggtag tggaaggaaa ggggtgcggg gcagcggaag gaagggtggg
      901 gcggggcgtg gcggggtctg tggctgggcg cgacgtcacc gacgttggag ccactccttt
      961 gaccatgtgt gcgtgtgtgt attattcgtg tctcgccact cgccggttgt ttttttcttt
     1021 ttatgctgcg ctctctctag cgccatctcg cttacgcatg ctcaacgcac cgcatgttgc
     1081 cgtttccttt tatgcgtcat tttggctcga aataggcaat tatttaaaca aagattagtc
     1141 aacgaaaacg ctaaaataaa taagtctaca atatggttac ttattgccat gtgtgtgcag
     1201 ccaacgatag caacaaaagc aacaacacag gtggctttcc ctctttcact ttttgtttgc
     1261 aagccgcgtg cgagcaagac ggcacgaccg gcaaacgcaa ttacgctgac aaagagcaga
     1321 cgaagttttg gcgaaaaaca tcaaggcgcc tgatacgaat gcatttgcaa taacaattgc
     1381 gatatttaat attgtttatg aagctgtttg acttcaaaac acacaaaaaa aaaaataaaa
     1441 caaattattt gaaagagaat taggaatcgg acgcttatcg ttagggtaac aacaagaaat
     1501 gcttactgag tcacagcctc tggaaaactg ccgcaagcca gagagagaga gaaaaagagg
     1561 gagagcagct tagaccgcat gtgcttgtgt gtgaggcgtc tctctcttcg tctctgttgc
     1621 gcaaacgcat agactgcact gaaaaaatcg attacctatt ttttatgaat gaatatttgc
     1681 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1741 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1801 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1861 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgaatgcc ctactagaag
     1921 atgtgtgttg cacaaaatgt ccctggaata accaatttga agtgcagata gcagtaaacg
     1981 taagctaata tgaatattat ttaactgtaa tgttttaata tcgctggaca ttactaataa
     2041 acccactata aacacatgta catatgtatg ttttggcata caatgagtag ttggggaaaa
     2101 aatgtgtaaa agcaccgtga ccatcacagc ataaagataa ccagctgaag tatcgaatat
     2161 gagtaacccc caaattgaat cacatgccgc aactgatagg acccatggaa gtacactctt
     2221 catggcgata tacaagacac acacaagcac gaacacccag ttgcggagga aattctccgt
     2281 aaatgaaaac ccaatcggcg aacaattcat acccatatat ggtaaaagtt ttgaacgcga
     2341 cttgagagcg gagagcattg cggctgataa ggttttagcg ctaagcgggc tttataaaac
     2401 gggctgcggg accagttttc atatcactac cgtttgagtt cttgtgctgt gtggatactc
     2461 ctcccgacac aaagccgctc catcagccag cagtcgtcta atccagagac cccggatcgg
     2521 ggtaccatca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     2581 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     2641 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     2701 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
     2761 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     2821 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     2881 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     2941 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
     3001 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
     3061 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     3121 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
     3181 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     3241 tacaagccaa gcttaatgaa catgtacaac ggacagatga acgccttcgg aggcggtggc
     3301 ggcggaggag ctggtggcct aaccggcggt atggtgccca atcgcatggg cggtggtgct
     3361 cccggtggcg gacccggcgg agcgggcatg ttccagcgca atcgctcggc cccatacccg
     3421 ggtttcaatg gtcatggacc cgccaacggt ggacagcgac gcatgaacgg tggtggtcct
     3481 agcatgggac ccggcggacc acgcaaccag gatggattcg gtggtcagaa cgggggccag
     3541 cgctcttcca atcacggcgc acacttgccc aagatcgttt ggtctgaggt gaatcttacg
     3601 ccattccgca agaactttta caagccgtgc gattcggtgc tggccaggac tgtcggtgaa
     3661 acagagacct tcctcaccag caatgagatc accatcaagg gcgatcaggt gcccacaccc
     3721 agtattgaat tcgaggaggg cggctttccc gattacgtga tgaacgagat ccgtaagcag
     3781 ggtttcgcca agccaacggc cattcaagcc cagggttggc ccattgctat gagtggacgg
     3841 gatctggtgg gcgtggccca gaccggatcg ggcaagacct tggcgtatgt tctgccggcg
     3901 gtggtgcaca tcaataacca gccgcgcctg gagcgcggtg atggacccat cgccctggtc
     3961 ctggctccca ctagagagtt ggcccagcag atccagcagg tagccattga gtttggcagc
     4021 aacacgcatg tgcgcaacac atgcatcttt ggtggagcgc ccaagggaca gcaggctagg
     4081 gatttggagc gcggcgtgga gattgtaatt gccacaccag gtcgcttgat tgatttcctc
     4141 gagcgcggca ccacctcgtt gaagagatgc acctatctgg tgctagacga ggccgatcgt
     4201 atgctcgaca tgggcttcga gccccagatc agaaagatca tgcagcagat ccgaccggac
     4261 cgtcaggttc tcatgtggtc agctacctgg cccaaagagg tgcgccagtt ggccgaggag
     4321 ttccttaata actacataca ggtgaacatt ggttcgcttt cgctgagcgc caatcacaac
     4381 atcctccaga tcgtggacgt gtgcgatgag aatgagaaat tgatgaagct gatcaagctg
     4441 ctaacagaca tctcggcgga gaacgagacc aagaccatca ttttcgtgga aaccaagaag
     4501 cgtgtggacg agatcacgcg caatatctcc cgccagggct ggcgcgcatg cgccatccac
     4561 ggcgataagt cgcggcagga gcgcgacttt gtgctctcga gcttccgcaa tggccggcac
     4621 tcgatactgg tggccaccga tgtggctgcc cgcggactcg acgttgacga tgtcaagttt
     4681 gtgatcaact atgattatcc tttgaactcg gaggactacg ttcatcgcac tggacgcacc
     4741 ggacgatcca ataacaccgg caccgcctac accctgttca cgcattccaa cgccaacaag
     4801 gccaacgact tgatccaggt gctgcgcgaa gccaaccaga ccatcaatcc caaactgatg
     4861 aacatggcca tgaacggtgg ctacaacaag cgcggtggcg gcatgggtgg cggctaccgt
     4921 ggcggcaatg gctatcaggg ccgcaatccg cagatgggcg gtggtggata caatggcggc
     4981 aacaactacc gcaacaacaa tggtcccggc gccaccatga atcgcaattc cttcaacggc
     5041 ggcagcgctg gcggaccacc acgcttcgat cagaagccgc gcacttctcc gccgaatcag
     5101 ggcggacagg gcggctaccg tggccaggga ggcggtggct accagggaca acagcagcag
     5161 cagcagcagc agaacggcgg agtgcccttc tctcgtttca atcccaatgc cgcttgcttc
     5221 gagcccaaca agccgcagaa tggtccggga tctggaccgc cacaggcgca gtaccagcag
     5281 cagcaacagg ctcaggctgc ccagcagcaa cacttacttc ctgatgcatc cgtgcaggcg
     5341 gcgatgacgg ctgcaaacta cggagttgat cagaagcgct cccgtttctc gccccacaac
     5401 atgaactttg ccaacatgcc accaccagcg atgaaccagc agcaggcggc ggcccagcag
     5461 caacagcaac aacagttgca aactccccag cagcaacagc agcagcagca aatgcaacag
     5521 ccacaggcct atggccagta ctcttcaatg tcctccagca tggcaaccgt gtcgctcaac
     5581 ggaggagctg cagcggtggc cagctcttac cgcgcccagt acgcagtgcc cacgcaggcg
     5641 gcaccctata tgatggcgaa cggcggcgac atctttgcct atccgccgcc tccgctgcct
     5701 gtgcagaact aggcggccgc tcgagtctag agggcccgcg gttcgaaggt aagcctatcc
     5761 ctaaccctct cctcggtctc gattctacgc gtaccggtca tcatcaccat caccattgag
     5821 tttaaacccg ctgatcagcc tcgactgtgc cttctaaggc ctgagctcgc tgatcagcct
     5881 cgatcgagga tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
     5941 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
     6001 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
     6061 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg
     6121 attatgatca gtcgaccgat gcccttgaga gccttcaacc cagtcagctc cttccggtgg
     6181 gcgcggggca tgactatcgt cgccgcactt atgactgtct tctttatcat gcaactcgta
     6241 ggacaggtgc cggcagcgct ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg
     6301 acgatgatcg gcctgtcgct tgcggtattc ggaatcttgc acgccctcgc tcaagccttc
     6361 gtcactggtc ccgccaccaa acgtttcggc gagaagcagg ccattatcgc cggcatggcg
     6421 gccgacgcgc tgggctacgt cttgctggcg ttcgcgacgc gaggctggat ggccttcccc
     6481 attatgattc ttctcgcttc cggcggcatc gggatgcccg cgttgcaggc catgctgtcc
     6541 aggcaggtag atgacgacca tcagggacag cttcaaggcc agcaaaaggc caggaaccgt
     6601 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     6661 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     6721 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     6781 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     6841 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     6901 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     6961 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     7021 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     7081 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     7141 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     7201 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     7261 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     7321 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     7381 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     7441 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     7501 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     7561 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     7621 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     7681 aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     7741 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     7801 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     7861 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     7921 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     7981 tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg
     8041 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     8101 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     8161 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     8221 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     8281 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     8341 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     8401 acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcaaga attaattcta
     8461 tattctaaaa acacaaatga tacttctaaa aaaaaat
//
