LOCUS       sequence-382849-        6232 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Regulates bacterial expression of target gene in 
            blue-light-activated manner. Based on Nakamurella multipartita PAL..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6232)
  AUTHORS   Ranzani AT, Wehrmann M, Kaiser J, Juraschitz M, Weber AM, 
            Pietruschka G, Gerken U, Mayer G, Moglich A
  TITLE     Light-Dependent Control of Bacterial Expression at the mRNA Level.
  JOURNAL   ACS Synth Biol. 2022 Oct 21;11(10):3482-3492. doi: 
            10.1021/acssynbio.2c00365. Epub 2022 Sep 21.
  PUBMED    36129831
REFERENCE   2  (bases 1 to 6232)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.2c00365"; journalName: "ACS Synth Biol"; date: 
            "2022-10-21- 21"; volume: "11"; issue: "10"; pages: "3482-3492"
COMMENT     Sequence Label: pAurora
FEATURES             Location/Qualifiers
     source          1..6232
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     229..248
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             1469..1498
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(1551..1569)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1565..1612
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(1666..1683)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     CDS             1827..2537
                     /codon_start=1
                     /gene="cI"
                     /product="phage lambda repressor"
                     /label=lambda repressor
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQ
                     SGVGALFNGINALNAYNAALLAKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEY
                     PVFSHVQAGMFSPELRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPD
                     GMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSV
                     VGKVIASQWPEETFG"
     CDS             2538..2570
                     /codon_start=1
                     /product="C-terminal peptide that mediates degradation in 
                     bacteria through the ClpXP and ClpAP proteases (McGinness 
                     et al., 2006)"
                     /label=ssrA tag (LVA)
                     /note="mutant LVA variant that confers accelerated 
                     degradation under some conditions (Andersen et al., 1998)"
                     /translation="AANDENYALVA"
     terminator      2618..2689
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2705..2732
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     RBS             2808..2830
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2849..2866
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             2876..2893
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     terminator      3073..3159
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3251..3278
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(3479..4270)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(4271..4362)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4410..5148)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     CDS             complement(5349..6227)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 tcatactccc gccattcaga gaagaaacca attgtccata ttgcatcaga cattgccgtc
       61 actgcgtctt ttactggctc ttctcgctaa ccaaaccggt aaccccgctt attaaaagca
      121 ttctgtaaca aagcgggacc aaagccatga caaaaacgcg taacaaaagt gtctataatc
      181 acggcagaaa agtccacatt gattatttgc acggcgtcac actttgctat gccatagcat
      241 ttttatccat aagattagcg gatcctacct ttttaaaaaa ttcatttgct aaacgcttca
      301 aattctcgta taattacttt tctccatacc cgttttttgg gctaacccgc cgaaacctaa
      361 ggaaatacat agtatgaaag ttaatcgtcc ggcagaacgt gccagctttg gtagctttgt
      421 tctggatgca ggtagcgcac gttttgttgg tagtgatgaa ctggcactgg ttctgggttt
      481 tgcaccgggt gatgttgttc tgacaccggc agttgttctg gcacatctgc atcctgatga
      541 tcgtctggaa tggcaggcag gtctgcagcg ttgtctggca accggtcgtc cggtggttgt
      601 taatcatctg ctgctgaccg cagaagcaga accgcgtccg gcaatgacaa ccctgaccgc
      661 actgaccgaa caggatcgtg tgcgtgcagt taccggtgtt attaccgatc tgagcgatcg
      721 tgttcgtcgt gcaaccgaag cagaaattcg tcaggcagtt cgtgcagcag cagcaacccg
      781 tagcgaaatt gatcaggcaa aaggtattgt tatggcagcc tttgatgttg atgcagatca
      841 ggcatttgca ctgctgaaat ggcatagcag ccagagcaat cgtaaactgc gtgatctggc
      901 caccggtatg attgaaggtc tggcagcagc caatagcgca ctgccgctgc gtcgtcgtct
      961 gagcaccgtt tttaccgata tgggttgtcc ggcaccgagc accaaaggtt ggaccgttcc
     1021 ggtgaccgat attggtctgc ctccgaccag cggtctgatt ccgacagcac tgctgcctgg
     1081 tattctgacc cgtgcagcac atgatgcaag cgttgcaatt accgttgcag atgttaccgc
     1141 accggatcag cctctggttt atgcaaatcc tgcatttgaa cgtctgaccg gttatgcagc
     1201 agccgaagtt ctgggtcgta attgtcgttt tctgcaggca gaaagcggtg atccgcatga
     1261 acgtagcgca attcgtagcg ccattgcaaa tggtgatgca gtgaccacac tgattcgtaa
     1321 ttttcgtcag gatggtcatg ccttttggaa cgaatttcat ctgagtccgg ttcgtaatgg
     1381 tgcaggtcgt gttacccatt acattggtta tcagctggat gtgaccgaac gtgttgaacg
     1441 tgatcagcag ctggaacagc tggcaagcga acagaaactg atttctgaag aagatctgta
     1501 agatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca
     1561 ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctcggccg
     1621 cactcgagag cttggctgtt ttggcggatg agagaagatt ttcagcctga tacagattaa
     1681 atcagaacgc agaagcggtc tgataaaaca gaatttgccg gtgttgacat aaataccact
     1741 ggcggtgata ctgagcacat cagcaggccc ctctagaaat aattttgttt aactttaaat
     1801 cttcagcagc gaggaggaga tataccatga gcacaaaaaa gaaaccatta acacaagagc
     1861 agcttgagga cgcacgtcgc cttaaagcaa tttatgaaaa aaagaaaaat gaacttggct
     1921 tatcccagga atctgtcgca gacaagatgg ggatggggca gtcaggcgtt ggtgctttat
     1981 ttaatggcat caatgcatta aatgcttata acgccgcatt gcttgcaaaa attctcaaag
     2041 ttagcgttga agaatttagc ccttcaatcg ccagagaaat ctacgagatg tatgaagcgg
     2101 ttagtatgca gccgtcactt agaagtgagt atgagtaccc tgttttttct catgttcagg
     2161 cagggatgtt ctcacctgag cttagaacct ttaccaaagg tgatgcggag agatgggtaa
     2221 gcacaaccaa aaaagccagt gattctgcat tctggcttga ggttgaaggt aattccatga
     2281 ccgcaccaac aggctccaag ccaagctttc ctgacggaat gttaattctc gttgaccctg
     2341 agcaggctgt tgagccaggt gatttctgca tagccagact tgggggtgat gagtttacct
     2401 tcaagaaact gatcagggat agcggtcagg tgtttttaca accactaaac ccacagtacc
     2461 caatgatccc atgcaatgag agttgttccg ttgtggggaa agttatcgct agtcagtggc
     2521 ctgaagagac gtttggcgct gcaaacgacg aaaactacgc tttagtagct taataacgct
     2581 gatagtgcta gtgtagatcg ctactagagc caggcatcaa ataaaacgaa aggctcagtc
     2641 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
     2701 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagtaac accgtgcgtg
     2761 ttgactattt tacctctggc ggtgataatg gttgctctag aaataatttt gtttaacttt
     2821 aagaaggaga tataccatgg gcagcagcca tcatcatcat catcacagca gcggcctggt
     2881 gccgcgcggc agccatatgg gatccgaatt cgagctccgt cgacaagctt gcggccgcac
     2941 tcgagcatga gatccctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact
     3001 cagaagtgaa acgccgtagc gccgatggta gtgtggggtc tccccatgcg agagtaggga
     3061 actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
     3121 tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac
     3181 gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat
     3241 caaattaagc agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttttg
     3301 tttatttttc taaatacatt caaatatgta tccgctcatg gacactgctt ccggtagtca
     3361 ataaaccggt aaaccagcaa tagacataag cggctattta acgaccctgc cctgaaccga
     3421 cgaccgggtc atcgtggccg gatcttgcgg cccctcggct tgaacgaatt gttagacatt
     3481 atttgccgac taccttggtg atctcgcctt tcacgtagtg gacaaattct tccaactgat
     3541 ctgcgcgcga ggccaagcga tcttcttctt gtccaagata agcctgtcta gcttcaagta
     3601 tgacgggctg atactgggcc ggcaggcgct ccattgccca gtcggcagcg acatccttcg
     3661 gcgcgatttt gccggttact gcgctgtacc aaatgcggga caacgtaagc actacatttc
     3721 gctcatcgcc agcccagtcg ggcggcgagt tccatagcgt taaggtttca tttagcgcct
     3781 caaatagatc ctgttcagga accggatcaa agagttcctc cgccgctgga cctaccaagg
     3841 caacgctatg ttctcttgct tttgtcagca agatagccag atcaatgtcg atcgtggctg
     3901 gctcgaagat acctgcaaga atgtcattgc gctgccattc tccaaattgc agttcgcgct
     3961 tagctggata acgccacgga atgatgtcgt cgtgcacaac aatggtgact tctacagcgc
     4021 ggagaatctc gctctctcca ggggaagccg aagtttccaa aaggtcgttg atcaaagctc
     4081 gccgcgttgt ttcatcaagc cttacggtca ccgtaaccag caaatcaata tcactgtgtg
     4141 gcttcaggcc gccatccact gcggagccgt acaaatgtac ggccagcaac gtcggttcga
     4201 gatggcgctc gatgacgcca actacctctg atagttgagt cgatacttcg gcgatcaccg
     4261 cttccctcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     4321 tgagcggata catatttgaa tgtatttaga aaaataaaca aatagctagc tcactcggtc
     4381 gctacgctcc gggcgtgaga ctgcggcggg cgctgcggac acatacaaag ttacccacag
     4441 attccgtgga taagcagggg actaacatgt gaggcaaaac agcagggccg cgccggtggc
     4501 gtttttccat aggctccgcc ctcctgccag agttcacata aacagacgct tttccggtgc
     4561 atctgtggga gccgtgaggc tcaaccatga atctgacagt acgggcgaaa cccgacagga
     4621 cttaaagatc cccaccgttt ccggcgggtc gctccctctt gcgctctcct gttccgaccc
     4681 tgccgtttac cggatacctg ttccgccttt ctcccttacg ggaagtgtgg cgctttctca
     4741 tagctcacac actggtatct cggctcggtg taggtcgttc gctccaagct gggctgtaag
     4801 caagaactcc ccgttcagcc cgactgctgc gccttatccg gtaactgttc acttgagtcc
     4861 aacccggaaa agcacggtaa aacgccactg gcagcagcca ttggtaactg ggagttcgca
     4921 gaggatttgt ttagctaaac acgcggttgc tcttgaagtg tgcgccaaag tccggctaca
     4981 ctggaaggac agatttggtt gctgtgctct gcgaaagcca gttaccacgg ttaagcagtt
     5041 ccccaactga cttaaccttc gatcaaacca cctccccagg tggttttttc gtttacaggg
     5101 caaaagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctactgaa
     5161 ccgctctaga tttcagtgca atttatctct tcaaatgtag cacctgaagt cagccccata
     5221 cgatataagt tgtaattctc atgttagtca gccccgcatg cataatgtgc ctgtcaaatg
     5281 gacgaagcag ggattctgca aaccctatgc tactccgtca agccgtcaat tgtctgattc
     5341 gttaccaatt atgacaactt gacggctaca tcattcactt tttcttcaca accggcacgg
     5401 aactcgctcg ggctggcccc ggtgcatttt ttaaataccc gcgagaaata gagttgatcg
     5461 tcaaaaccaa cattgcgacc gacggtggcg ataggcatcc gggtggtgct caaaagcagc
     5521 ttcgcctggc tgatacgttg gtcctcgcgc cagcttaaga cgctaatccc taactgctgg
     5581 cggaaaagat gtgacagacg cgacggcgac aagcaaacat gctgtgcgac gctggcgata
     5641 tcaaaattgc tgtctgccag gtgatcgctg atgtactgac aagcctcgcg tacccgatta
     5701 tccatcggtg gatggagcga ctcgttaatc gcttccatgc gccgcagtaa caattgctca
     5761 agcagattta tcgccagcag ctccgaatag cgcccttccc cttgcccggc gttaatgatt
     5821 tgcccaaaca ggtcgctgaa atgcggctgg tgcgcttcat ccgggcgaaa gaaccccgta
     5881 ttggcaaata ttgacggcca gttaagccat tcatgccagt aggcgcgcgg acgaaagtaa
     5941 acccactggt gataccattc gcgagcctcc ggatgacgac cgtagtgatg aatctctcct
     6001 ggcgggaaca gcaaaatatc acccggtcgg caaacaaatt ctcgtccctg atttttcacc
     6061 accccctgac cgcgaatggt gagattgaga atataacctt tcattcccag cggtcggtcg
     6121 ataaaaaaat cgagataacc gttggcctca atcggcgtta aacccgccac cagatgggca
     6181 ttaaacgagt atcccggcag caggggatca ttttgcgctt cagccatact tt
//
