LOCUS       sequence-382850-        7200 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pSLI-TGD/TurboID, C-terminal tag of recodonized TurboID for 
            Plasmodium falciparum.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7200)
  AUTHORS   Bennink S, Lindner SE
  TITLE     Bennink et al - PfTurboID
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7200)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pSL1423
FEATURES             Location/Qualifiers
     source          1..7200
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1020..1048)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1643..1663
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             1713..1766
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             1761..2561
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="GPMIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQG
                     RPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEE
                     HQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQD
                     IALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1821..1840)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2431..2450
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4516..4535)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4517..4535)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4542..4559)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4542..4558)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4551..4573)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4767..4786)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4886..4908
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4946..4964)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5032..5136
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5137..5205,5206..5997)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5355..5374)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6168..6756
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6657..6676
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6910..6927
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7044..7065
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7080..7085,7086..7103,7104..7110)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7118..7134
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7123..7145
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7142..7158
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7142..7158
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        7176..7194
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     7176..7193
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 aagctgcggc cgctctcgag actagtatga aagataacac ggttccctta aaattgatag
       61 cccttcttgc caatggtgag tttcattccg gggaacaact tggcgaaact ctgggaatga
      121 gcagggcagc gattaacaag catatccaaa ccttgagaga ctggggagta gatgtgttca
      181 ccgtgcctgg taaaggttat tctttgccgg aacctatccc tctattaaat gcgaaacaga
      241 tactaggaca actggacggt ggtagtgttg ccgtattgcc ggttgttgac tcaaccaatc
      301 aatacttgct ggacagaatt ggcgaactta aaagcggtga tgcgtgtatt gcagaatatc
      361 agcaagcagg gagaggctca agaggtagga aatggttttc accttttggc gccaatcttt
      421 acttaagtat gttctggcgt ttgaagagag gacctgcggc tattggttta ggtcccgtta
      481 tcgggatagt tatggctgaa gcgctacgta aacttggggc cgacaaggtc agagtgaagt
      541 ggcctaatga tctatacttg caagatagaa agctggcggg tatcctggtg gaactagcgg
      601 gaatcacagg ggacgctgcc caaatagtaa tcggtgctgg tataaatgtc gcaatgcgta
      661 gagtcgagga atcagtggtg aaccagggct ggatcacgtt gcaagaggcg ggcataaacc
      721 tggaccgtaa cacccttgca gcgaccctaa taagagaact acgtgctgca ttggagcttt
      781 ttgagcagga gggcctggct ccgtacctac ccaggtggga gaaattagac aatttcataa
      841 accgtccagt caaactaata attggagata aggagatttt cggtatttct cgtgggatag
      901 acaagcaggg agcgttgtta ttagaacagg atggtgtcat taaaccttgg atgggcggtg
      961 aaattagtct tcgtagtgcc gaaaagtcta gtagtaaagg agaagaactt ttcactggag
     1021 ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg
     1081 gagagggtga aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg
     1141 gaaaactacc tgttccatgg ccaacacttg tcactacttt cgcgtatggt cttcaatgct
     1201 ttgcgagata cccagatcat atgaaacagc atgacttttt caagagtgcc atgcccgaag
     1261 gttatgtaca ggaaagaact atatttttca aagatgacgg gaactacaag acacgtgctg
     1321 aagtcaagtt tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgatttta
     1381 aagaagatgg aaacattctt ggacacaaat tggaatacaa ctataactca cacaatgtat
     1441 acatcatggc agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt agacacaaca
     1501 ttgaagatgg aagcgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg
     1561 gccctgtcct tttaccagac aaccattacc tgtccacaca atctgccctt tcgaaagatc
     1621 ccaacgaaaa gagagaccac atggtccttc ttgagtttgt aacagctgct gggattacac
     1681 atggcatgga tgagctctac aaagtcgacg gagaaggaag aggaagttta ttaacatgtg
     1741 gagatgtaga agaaaatcca ggaccaatga ttgaacaaga tggattgcac gcaggttctc
     1801 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
     1861 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
     1921 acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca
     1981 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
     2041 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
     2101 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
     2161 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
     2221 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
     2281 ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct
     2341 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
     2401 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
     2461 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
     2521 agcgcatcgc cttctatcgc cttcttgacg agttcttcta actcgaggga tatggcagct
     2581 taatgttcgt ttttcttatt tatatattta taccaattga ttgtatttat aactgtaaaa
     2641 atgtgtatgt tgtgtgcata tttttttttg tgcatgcaca tgcatgtaaa tagctaaaat
     2701 tatgaacatt ttattttttg ttcagaaaaa aaaaacttta cacacataaa atggctagta
     2761 tgaatagcca tattttatat aaattaaatc ctatgaattt atgaccatat taaaaattta
     2821 gatatttatg gaacataata tgtttgaaac aataagacaa aattattatt attattatta
     2881 tttttactgt tataattatg ttgtctcttc aatgattcat aaatagttgg acttgatttt
     2941 taaaatgttt ataatatgat tagcatagtt aaataaaaaa agttgaaaaa ttaaaaaaaa
     3001 acatataaac acaaatgatg ttttttcctt caatttcgat atcaatttat agaaacaaaa
     3061 tatatacttg tataatttta tttttttata taaatcatta catatataat ggtgcatgat
     3121 aaaagaaaaa aataatattt ataaaaaaaa agaaaaataa aacaaaaaaa gaaaaaaaaa
     3181 aaaaaaaaaa taaaaaaata aataatataa tttataatta tatattcttg tcacaataaa
     3241 aatatatata tatatatata tatttataat atgtatattt taaactagaa aaggaataac
     3301 taatatttta tttattatca ttcaagattt atattttata ataataaata cctaatagaa
     3361 atatatcagg atccatgcat ggttcgctaa actgcatcgt tgctgtgtcc cagaacatgg
     3421 gcatcggcaa gaacggggac tacccctggc caccgctcag gaacgaattt agatatttcc
     3481 agagaatgac cacaacctct tcagtagaag gtaaacagaa tctggtgatt atgggtaaga
     3541 agacctggtt ctccattcct gagaagaatc gacctttaaa gggtagaatt aatttagttc
     3601 tcagcagaga actcaaggaa cctccacaag gagctcattt tctttccaga agtctagatg
     3661 atgccttaaa acttactgaa caaccagaat tagcaaataa agtagacatg gtctggatag
     3721 ttggtggcag ttctgtttat aaggaagcca tgaatcaccc aggccatctt aaactatttg
     3781 tgacaaggat catgcaagac tttgaaagtg acacgttttt tccagaaatt gatttggaga
     3841 aatataaact tctgccagaa tacccaggtg ttctctctga tgtccaggag gagaaaggca
     3901 ttaagtacaa atttgaagta tatgagaaga atgattaagc ttatttaata atagattaaa
     3961 aatattataa aaataaaaac ataaacacag aaattacaaa aaaaatacat atgaattttt
     4021 ttttgtaatc ttccttataa atatagaata atgaatcata taaaacatat cattattcat
     4081 ttatttacat ttaaaattat tgtttcagta tctttaattt attatgtata tataaaaata
     4141 acttacaatt ttattaataa gcaatatatg tttattaatt catgttttgt aatttatggg
     4201 atagcgattt tttttactgt ctgtattttt cttttttaat tatgttttaa ttgtatttta
     4261 tttttattat tgttcttttt atagtattat tttaaaacaa aatgtatttt ctaagaactt
     4321 ataataataa taaatataaa ttttaataaa aattatattt atcttttaca atatgaacat
     4381 aaagtacaac attaatatat agcttttaat atttttattc ctaatcatgt aaatcttaaa
     4441 tttttctttt taaacatatg ttaaatattt atttctcatt atatataaga acatatttat
     4501 taaatctaga attcgcccta tagtgagtcg tattacaatt cactggccgt cgttttacaa
     4561 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     4621 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     4681 agcctgaatg gcgaatggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     4741 tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     4801 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     4861 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     4921 tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     4981 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     5041 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     5101 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     5161 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     5221 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     5281 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     5341 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     5401 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     5461 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     5521 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     5581 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     5641 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     5701 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     5761 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     5821 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     5881 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     5941 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     6001 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     6061 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     6121 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     6181 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     6241 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     6301 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     6361 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     6421 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     6481 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     6541 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     6601 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     6661 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     6721 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     6781 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     6841 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     6901 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     6961 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     7021 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     7081 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     7141 acaggaaaca gctatgacca tgattacgcc aagctattta ggtgacacta tagaatactc
//
