LOCUS       sequence-38289-e         757 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Zhao Y, Araki S, Wu J, Teramoto T, Chang YF, Nakano M, Abdelfattah 
            AS, Fujiwara M, Ishihara T, Nagai T, Campbell RE
  TITLE     An Expanded Palette of Genetically Encoded Ca2+ Indicators.
  JOURNAL   Science. 2011 Sep 30;333(6051):1888-91. doi: 10.1126/science.1208592
  PUBMED    21903779
REFERENCE   2  (bases 1 to 757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2011-09-30- 30"; volume: "333"; 
            issue: "6051"; pages: "1888-91"
COMMENT     Sequence Label: pTorPE-R-GECO1
FEATURES             Location/Qualifiers
     source          1..757
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             71..82
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             237..254
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             258..290
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(294..302,303..317)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             345..401
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKAGHAVRAIGRLSS"
ORIGIN
        1 tttatcgcaa ctctctactg tttctccata cccgtttttt gggctaacag gaggaattaa
       61 ccatgggttt aaagaggaga aaggtcatga acaataacga tctctttcag gcatcacgtc
      121 ggcgttttct ggcacaactc ggcagcttaa ccgacgccgg gacgctgggg ccgtcattgt
      181 taacgccgcg acgtgcgact gcggcgcaag cggcgactga cgcttctaga ggttctcatc
      241 atcatcatca tcatggtatg gctagcatga ctggaggaca gcaaatgggt cgggatctgt
      301 acgacgatga tgataaggat ctcgccacaa tggtcgactc ttcacgtcgt aagtggaata
      361 aggcaggtca cgcagtcaga gctataggtc ggctgagctc acccgtggtt tccgagcgga
      421 tgtaccccga ggacggcgcc ctgaagagcg agatcaagaa ggggctgagg ctgaaggacg
      481 gcggccacta cgccgccgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc
      541 ccggcgccta catcgtcgac atcaagttgg acatcgtgtc ccacaacgag gactacacca
      601 tcgtggaaca gtgcgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt
      661 acaagggagg tacaggcggg agtctggtga gcaagggcga ggaggataac atggccatca
      721 tcaaggagtt catgcgcttc aaggtgcaca tggaggg
//
