LOCUS       sequence-38291-e         790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Zhao Y, Araki S, Wu J, Teramoto T, Chang YF, Nakano M, Abdelfattah 
            AS, Fujiwara M, Ishihara T, Nagai T, Campbell RE
  TITLE     An Expanded Palette of Genetically Encoded Ca2+ Indicators.
  JOURNAL   Science. 2011 Sep 30;333(6051):1888-91. doi: 10.1126/science.1208592
  PUBMED    21903779
REFERENCE   2  (bases 1 to 790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2011-09-30- 30"; volume: "333"; 
            issue: "6051"; pages: "1888-91"
COMMENT     Sequence Label: pTorPE-G-GECO1
FEATURES             Location/Qualifiers
     source          1..790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(53..493)
                     /codon_start=1
                     /product="calcium-binding protein"
                     /label=calmodulin
                     /translation="DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEA
                     ELQDMINEVDADGDGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYIGAAEL
                     RHVMTNLGEKLTDEEVDEMIRVADIDGDGQVNYEEFVQMMTAK"
     primer_bind     609..628
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 agcttctaag ctgctaaagc gtagttccgt cgtttgccgc cccgggacca cccttcgctg
       61 tcatcatttg tacaaactct tcgtagttta cctgaccatc cccatcgatg tctgctaccc
      121 tgatcatttc atcaacctct tcatctgtta acttctctcc aaggtttgtc atcacgtggc
      181 gaagctctgc tgcgccgatg tagccattgc cgtccttatc aaacacacgg aacgcttctc
      241 taatttcttc ttcactgtct gtgtctttca tttttcttgc catcattgtc aggaactcag
      301 ggaagtcgat tgtgccgtca ccgtcggcat ctacttcatt gatcatgtcc tgcagctctg
      361 cttctgtggg gttctgcccc agagaccgca tcaccgtccc cagctccttg gttgttatcg
      421 tcccatcccc gtccttgtca aatagggaga aagcctcttt aaattctgcg atctgctctt
      481 cagtcagttg gtcacgcgtg ttgtactcca gcttgtgccc caggatgttg ccgtcctcct
      541 tgaagtcgat gcccttcagc tcgatgcggt tcaccagggt gtcgccctcg aacttcacct
      601 cggcgcgggt cttgtagttg ccgtcgtcct tgaagaagat ggtgcgctcc tggatgtagc
      661 cttcgggcat ggcggacttg aagaagtcgt gctgcttcat gtggtcgggg tagcggctga
      721 agcactgcac gccgtaggtc agggtggtca cgagggtggg ccagggcacg ggcagcttgc
      781 cggtggtgca
//
