LOCUS       sequence-38292-e         915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Zhao Y, Araki S, Wu J, Teramoto T, Chang YF, Nakano M, Abdelfattah 
            AS, Fujiwara M, Ishihara T, Nagai T, Campbell RE
  TITLE     An Expanded Palette of Genetically Encoded Ca2+ Indicators.
  JOURNAL   Science. 2011 Sep 30;333(6051):1888-91. doi: 10.1126/science.1208592
  PUBMED    21903779
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2011-09-30- 30"; volume: "333"; 
            issue: "6051"; pages: "1888-91"
COMMENT     Sequence Label: pTorPE-G-GECO1
FEATURES             Location/Qualifiers
     source          1..915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             71..82
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             237..254
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             258..290
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(294..302,303..317)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             345..401
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     614..635
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tttatcgcaa ctctctactg tttctccata cccgtttttt gggctaacag gaggaattaa
       61 ccatgggttt aaagaggaga aaggtcatga acaataacga tctctttcag gcatcacgtc
      121 ggcgttttca ggcacaactc ggcggcttaa ccgtcgccgg gatgctgggg ccgtcattgt
      181 taacgccgcg acgtgcgact gcggcgcaag cggcgactga cgcttctaga ggttctcatc
      241 atcatcatca tcatggtatg gctagcatga ctggtggaca gcaaatgggt cgggatctgt
      301 acgacgatga tgataaggat ctcgccacaa tggtcgactc atcacgtcgt aagtggaata
      361 agacaggtca cgcagtcaga gctataggtc ggctgagctc actcgagaac gtctatatca
      421 aggccgacaa gcagaagaac ggcatcaagg cgaacttcaa gatccgccac aacatcgagg
      481 acggcggcgt gcagctcgcc taccactacc agcagaacac ccccatcggc gacggccccg
      541 tgctgctgcc cgacaaccac tacctgagcg tgcagtccat actttcgaaa gaccccaacg
      601 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
      661 tggacgagct gtacaagggc ggtaccggag ggagcatggt gagcaagggc gaggagctgt
      721 tcaccggggt ggtgcccatc caggtcgagc tggacggcga cgtaaacggc cacaagttca
      781 gcgtgtccgg cgagggtgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
      841 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg
      901 tgcagtgctt cagcc
//
