LOCUS       sequence-382945-       10797 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of Csm complex from single promoter; RFP 
            backbone (Used for RNA knockdown).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10797)
  AUTHORS   Colognori D, Trinidad M, Doudna JA
  TITLE     Precise transcript targeting by CRISPR-Csm complexes.
  JOURNAL   Nat Biotechnol. 2023 Jan 23. doi: 10.1038/s41587-022-01649-9.
  PUBMED    36690762
REFERENCE   2  (bases 1 to 10797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2023 Jan 23. doi: 10.1038/s41587-022-01649-9."
COMMENT     Sequence Label: pDAC439
FEATURES             Location/Qualifiers
     source          1..10797
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        78..381
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..585
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     536..556
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      622..631
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             631..654
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             661..681
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1759..1812
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             1813..1836
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1843..1863
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2767..2820
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             2821..2844
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2851..2871
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3538..3591
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3592..3615
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3622..3642
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    4181..4262
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4273..4290)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        4390..4638
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     4390..4410
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     4561..4580
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     rep_origin      complement(4802..5390)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4882..4901)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5561..6352,6353..6421))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6184..6203
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6422..6526)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    6559..6640
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(9037..9057)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(9064..9087)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(9094..9111)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(9112..9165)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(9901..9921)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(9928..9951)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(9952..10005)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(10006..10713)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     10550..10568
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      10710..10719
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 acctcgacat actcgagtag ttattaatag taatcaatta cggggtcatt agttcatagc
       61 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
      121 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
      181 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
      241 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
      301 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
      361 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
      421 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
      481 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
      541 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt
      601 cagatccgct agggatccgc cgccaccatg gattacaaag acgatgacga taagatggcg
      661 cctaagaaga aacgcaaagt gcggggcatg aaaaatgact accggacctt caagctgagc
      721 ctgctgaccc tggctcctat ccacatcggc aacggcgaga agtacaccag cagagaattc
      781 atctacgaga acaagaagtt ctacttcccc gacatgggca agttctacaa caagatggtg
      841 gaaaagagac tggccgagaa gttcgaggcc ttcctgatcc agaccagacc caacgccaga
      901 aacaaccggc tgatttcttt tctgaacgac aacagaatcg ccgaaagatc ttttggcggc
      961 tacagcatca gtgaaaccgg cctggaatct gataagaacc ctaacagcgc cggagctatc
     1021 aacgaggtga acaaattcat ccgggacgcc ttcggaaatc cttacatccc aggcagcagc
     1081 ctgaagggcg ccatccgcac catcctgatg aacaccacac ctaagtggaa caacgagaac
     1141 gccgtgaacg acttcggcag attcccaaag gaaaacaaga acctgatccc ttggggacct
     1201 aagaaaggca aggaatacga cgacctgttc aacgccatca gagtgtccga cagcaagccc
     1261 ttcgacaaca aaagcctgat cctcgtgcag aagtgggact acagcgccaa aaccaacaag
     1321 gccaagcctc tgcctctgta cagagagtct atcagccctc tgaccaagat cgagttcgag
     1381 ataacaacaa ccactgatga ggccggcaga ctgatcgagg aactgggaaa gcgggcccag
     1441 gccttttata aggactacaa ggcctttttc ctgtctgaat tccctgatga taagatccag
     1501 gctaatctgc aataccccat ctacctgggc gccggcagcg gcgcttggac aaagaccctg
     1561 tttaagcagg ccgacggcat cctgcagcgg agatactcca gaatgaaaac caagatggtc
     1621 aagaagggcg tgctgaagct gacaaaggcc cctctgaaaa cagtgaagat ccccagcggc
     1681 aaccacagcc tggtgaagaa tcacgagagc ttctacgaga tgggcaaagc caacttcatg
     1741 atcaaggaaa tcgacaagga aggaaggggc tccctcctca cttgtggaga tgtcgaagaa
     1801 aatcctggac ctgattacaa agacgatgac gataagatgg cgcctaagaa gaaacgcaaa
     1861 gtgcggggca tgacttacaa gctctacatt atgacctttc aaaacgccca cttcggttcc
     1921 ggcactctgg actcatcgaa gctgaccttc tccgcggata gaatcttctc ggcactcgtg
     1981 ctcgaggctc tgaagatggg aaagctcgac gccttcttgg ccgaggccaa ccaggataag
     2041 ttcactctga ccgacgcgtt cccattccaa ttcggtcctt tcctgccgaa accgattggt
     2101 taccccaagc acgaccagat cgaccagtct gtggacgtga aggaagtccg ccgccaagcg
     2161 aagctgtcca aaaagctcca gttcctggct ctggaaaacg tcgacgacta cctgaacgga
     2221 gagctgtttg agaatgagga acacgccgtg atcgacacag tgaccaagaa ccagccccat
     2281 aaagatgata atctgtacca agtggccacc actcggttct cgaacgacac ctccctttac
     2341 gtgatcgcca acgaatccga tctgctgaac gaactgatga gcagccttca gtactccggg
     2401 ctgggcggca aaaggtcctc aggattcggc agatttgagc tggacatcca gaacattccc
     2461 ttggaactgt ccgaccggct gacgaagaac cacagcgaca aggtcatgtc acttaccacc
     2521 gccctcccgg tggacgctga tctcgaggaa gcgatggaag atggccatta cctgttgacc
     2581 aagtcgtccg gattcgcatt ctcccacgcc accaacgaaa actatcggaa gcaggacctg
     2641 tacaagttcg cctccgggag caccttcagc aagactttcg agggacagat cgtggacgtg
     2701 cgccctctcg atttccctca cgccgtgctg aactacgcca agccgctgtt ctttaagctc
     2761 gaagtcgagg gcagaggaag tctgctaaca tgcggtgacg tcgaggagaa tcctggacct
     2821 gattacaaag acgatgacga taagatggcg cctaagaaga aacgcaaagt gcggggcatg
     2881 accttcgcca agatcaaatt cagcgcccag atccggctgg aaaccggcct gcacatcgga
     2941 ggatctgatg cctttgccgc tatcggcgcc atcgacagcc ctgtgatcaa ggaccccatc
     3001 accaacctgc ctatcatccc cggctctagc ctgaagggca agatgagaac actgctggcc
     3061 aaggtgtaca acgaaaaggt ggccgagaag cctagcgacg acagcgacat cctgagcaga
     3121 ctgttcggaa atagcaagga taagcggttc aagatgggca gactgatctt ccgggacgcc
     3181 ttcctgagca acgccgacga gctggattct ctgggcgtgc ggagctacac cgaggtgaag
     3241 ttcgagaaca ccatcgatag aatcaccgcc gaggccaatc ctagacagat cgagagagcc
     3301 attcggaact caacattcga cttcgagctg atctacgaga tcactgatga gaatgagaac
     3361 caggtcgagg aagatttcaa ggtgatcaga gacggcctga agctgctgga actggactac
     3421 ctgggcggaa gcggctccag aggctacggc aaagtggctt ttgagaacct gaaagccacc
     3481 acagtgttcg gcaactacga cgtgaaaacc ctgaacgagc tgctgaccgc cgaagtggag
     3541 gggcgggggt ctttgttgac ttgcggggat gttgaggaga acccagggcc agattacaaa
     3601 gacgatgacg ataagatggc gcctaagaag aaacgcaaag tgcggggcat gaccatcctg
     3661 accgacgaga actacgtgga catcgccgag aaagccatcc tgaagctgga aagaaacacc
     3721 agaaatagaa agaaccctga tgccttcttc ctgaccacat ctaagctgcg gaacctgctg
     3781 agcctgacaa gcaccctgtt cgacgagagc aaggtgaagg aatacgacgc cctgctggac
     3841 agaatcgctt atctgagagt gcagttcgtg taccaggccg gcagagagat cgccgtgaaa
     3901 gatctgatcg agaaggccca gatcctggaa gctctgaaag agatcaagga ccgggaaacc
     3961 ctgcagagat tctgcagata catggaagcc ctggtggcct acttcaagtt ctacggcggc
     4021 aaggactgag ctagcgcggc cgcatcgata agcttgtcga cgatatctcc agaggatcat
     4081 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
     4141 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
     4201 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     4261 gccccgagct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     4321 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     4381 gaaactagtg agggcctatt tcccatgatt ccttcatatt tgcatatacg atacaaggct
     4441 gttagagaga taattagaat taatttgact gtaaacacaa agatattagt acaaaatacg
     4501 tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg
     4561 gactatcata tgcttaccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg
     4621 tggaaaggac gaaacaccga tataaaccta attacctcga gaggggacgg aaacccgtct
     4681 tcgatgaagc gattcagaag acttgatata aacctaatta cctcgagagg ggactttttt
     4741 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     4801 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     4861 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     4921 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     4981 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     5041 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     5101 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     5161 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     5221 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     5281 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     5341 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     5401 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     5461 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     5521 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     5581 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     5641 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     5701 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     5761 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     5821 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     5881 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     5941 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     6001 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     6061 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     6121 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac
     6181 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     6241 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     6301 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     6361 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     6421 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     6481 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     6541 aagtgccacc tgacgtcggc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
     6601 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca
     6661 ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta
     6721 caaatgtggt atggctgatt atgatcctct agattaatcc tttctgattt cgtagatgta
     6781 cagcaggagc gcgagctcgg cttccttccg gtccttatcg ttcttgttgg tgtaccagga
     6841 gtagaacagg ttcttgaagg tcttgaactt gtccctgtca gtctcccggg tcagttcttc
     6901 caggcgagtg aggtaatagg cgaggcgtgc catattcatg cggtcgtggt tcctcaaaag
     6961 ctcaataagc ttatagatga agttctttcc cctctcgtct tgatggttga agaagtatct
     7021 aatctgttcc agtttgtcgt cgtacacgtt agtgatgaac ctatcaaact tgaaagtgta
     7081 gtcgcttgag aacagcgaga tggagtcctt ttcgttgccc ttggcggcct cttccagttc
     7141 cccggtctgg tgagccatca ggctaatagg agtcttgtcg gcgaacaacc ctatccccgc
     7201 ggagagggtg agcttcccgt tggtccactt gatgaagttt tcgcgaagtt ccacagtgaa
     7261 cgcgatgata tcctgccacg atccaatggc gaacacgtca tcgccgcccg cgtagataat
     7321 gctcagcttc ttgtcggagg cgaactggtt aatgtacact ttgaagaaca gcgacatgct
     7381 ccgggagaat gtggccgatc ttgacagagt ggagtattgt ccgtttccct gctggctgaa
     7441 accggccatg aaggcggcgc ccaagtcatc cacgtcgagc cggaccacgg ccagtctctt
     7501 gatgcctagg ccgttctcgt tcttgctcag ggcggcgtag ttgtagatct cgtcgcactg
     7561 gtaatccccc acgaacacat gcgtagcctt cacggtaccg gccttatagt cattcttcac
     7621 gtagacccgg ctgaacgctt cttgggacag cttttcgaat gccacgccct ttaagcacgc
     7681 gtttggtcca atcggcagcc cctcattttc ggtaatgatg aagtggtcat gggcaatttc
     7741 tttcgagaac tggtacagtc cccggcaaat gtcacagact ttctggtcgt ggtaggacac
     7801 caggttctcc acggagtggc aaatctcgca ctctctctct gaggactttc caccgcgatt
     7861 caggagcatc agtgtctggt agtcgtatct ggagattttc tttttggaga tcatgcgcga
     7921 agccttttgg tacacttggc ggtaggactc ggggctattc agctcggaca tgatgtcctt
     7981 ggccgcgaag gaaccccagc caaaggccac atagaggcgg gtctggaagt ttgccaacag
     8041 gaactggttg aaatccttct caaactgcac cagggtttcc acagtctttt cggtgttggc
     8101 caggacgaag taggcgtgtc cgccgccgac gtaaagcatg ttagccctgt tcagtcccag
     8161 cttgtccagc aggctatcgg cgatgtactc ggacataaag tccaggtaga ggctccgggc
     8221 cttcagctgc ttcgccgcgc cgttagttgc gatgttaatg ttgtagataa agtcctggat
     8281 tcccgacagg tcgaaggagg ccagcaggaa ggcttcttct tcatagaacg ctgacacttt
     8341 ggtgaacagg tcctctttgt agttgtgccg acccttgtcc tccaggtagt cgtagatcgc
     8401 cagagcgaag gcagcagtca agcgggaatg gtcggccagg gagatgtcgg cgatttcctt
     8461 agtgttggtg cttgacggca cgaaggagag agtagcctcg aaaaggttga gcagggagtc
     8521 aatctggact tggttgaact cgaactcggc cagttcgttc ttaatccggg tggcgatggc
     8581 agcgtaatcg cccttgctaa agggttcgta agtggcggac gcgaagttgg gcttcgactt
     8641 gagattcagc acggtaggct tgaagtaccg cttatcggtc tgcgctccga acacgttaaa
     8701 gatgtcggcc tggttcgtgt aggtgtccca gatctttgcg gaggtatctt cgtctgactc
     8761 ttcgttggat tgccggcggt cgacaccgga ggcgatgttg tcggcaatgt aggtgatgta
     8821 agccaggtga tcgttgccga gcttatcaga ctggtagttg gccatatggt accggatctg
     8881 atccgagatg acttggttgt cggcgatctc gtcgaaccag tcggcgccca caagtgcgtg
     8941 tttcttccgc tctccggttg ctcgctggat gacctttccg atgtcgtgca gcagggctcc
     9001 gtaaaacaga tcaatctttt ctttcttcat gccccgcact ttgcgtttct tcttaggcgc
     9061 catcttatcg tcatcgtctt tgtaatcgcc ggaatggtga tggtgatggt gtggtcctgg
     9121 attttcttca acatctccac aagttagcaa acttcctctt ccttcgtcct ttctttcttc
     9181 cagcttgatg ccgcccattc ccaggctggt tttcatgccc aggccgctgt actcgccgaa
     9241 ggtcagcagc atcttcacgt aggccttcag tgtctgggct ccctgcacct tgaaggtcag
     9301 cttgccccgg aaggcgggga ttctctgtct atgcactctg aagtagcttg tttccagtct
     9361 gtaattggtg atggtgctgt gctcgctcag gtagtccagg gtatcttcct cgatctcagg
     9421 ctggttttcc accagtctgc cgtacttctg catcaggctc tgaaagatca gccgcatgct
     9481 ggggaagatc acgtagctgc cctggctttt aaagcctgta ggtgtctcga agatgatgct
     9541 gaagtaggtc tggttttcct cgctgttgaa gatctcgagc agcttatcag cgcccagctc
     9601 ctggatttcc accttttcca cagacaactt aggcaggtcg ttcagggaca cttccttgat
     9661 ctgcagcagg gtcatgaaca ccttgtcctc gatgtcgtct gtcagcagat gcacgaccca
     9721 ctgggtgttc tctttgccct ggatcacctt tgtggcgtag gggttggtct gctgctggtg
     9781 caggtagtcc acgtagtcgc tatccagctg ttccatcagg aagccgtgga atttcacggc
     9841 caggtcctga gcagggtggt cgatccgctt aaaggtgaac acgagctttt tcatgccccg
     9901 cactttgcgt ttcttcttag gcgccatctt atcgtcatcg tctttgtaat cggggccggg
     9961 gttctcctcc acgtcgccgc aggtcagcag gctgcctctg ccctccttgt acagctcgtc
    10021 catgccgccg gtggagtggc ggccctcggc gcgttcgtac tgttccacga tggtgtagtc
    10081 ctcgttgtgg gaggtgatgt ccaacttgat gttgacgttg taggcgccgg gcagctgcac
    10141 gggcttcttg gccttgtagg tggtcttgac ctcagcgtcg tagtggccgc cgtccttcag
    10201 cttcagcctc tgcttgatct cgcccttcag ggcgccgtcc tcggggtaca tccgctcgga
    10261 ggaggcctcc cagcccatgg ttttcttctg cattacgggg ccgtcggagg ggaagttggt
    10321 gccgcgcagc ttcaccttgt agatgaactc gccgtcctgc agggaggagt cctgggtcac
    10381 ggtcaccacg ccgccgtcct cgaagttcat cacgcgctcc cacttgaagc cctcggggaa
    10441 ggacagcttc aagtagtcgg ggatgtcggc ggggtgcttc acgtaggcct tggagccgta
    10501 catgaactga ggggacagga tgtcccaggc gaagggcagg gggccaccct tggtcacctt
    10561 cagcttggcg gtctgggtgc cctcgtaggg gcggccctcg ccctcgccct cgatctcgaa
    10621 ctcgtggccg ttcacggagc cctccatgtg caccttgaag cgcatgaact ccttgatgat
    10681 ggccatgtta tcctcctcgc ccttgctcac catggtggcg gcaccggtga attctccagg
    10741 cgatctgacg gttcactaaa cgagctctgc ttatataggc ctcccaccgt acacgcc
//
