LOCUS       sequence-382946-       11591 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of Csm complex from separate promoters; RFP 
            backbone (Used for RNA knockdown).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11591)
  AUTHORS   Colognori D, Trinidad M, Doudna JA
  TITLE     Precise transcript targeting by CRISPR-Csm complexes.
  JOURNAL   Nat Biotechnol. 2023 Jan 23. doi: 10.1038/s41587-022-01649-9.
  PUBMED    36690762
REFERENCE   2  (bases 1 to 11591)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2023 Jan 23. doi: 10.1038/s41587-022-01649-9."
COMMENT     Sequence Label: pDAC435
FEATURES             Location/Qualifiers
     source          1..11591
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        25..228
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             268..285
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             292..315
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             322..342
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2626..2673
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        2704..2907
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      2938..2947
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2947..2970
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2977..2997
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    3385..3432
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        3463..3666
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      3697..3706
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3706..3729
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3736..3756
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    4426..4473
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        4504..4707
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      4738..4747
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4747..4770
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4777..4797
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5704..5751
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        5782..5985
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      6016..6025
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6025..6048
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             6055..6075
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7156..7203
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        7234..7437
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      7468..7477
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7477..7500
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             7507..7527
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8266..8313
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        8344..8547
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      8578..8587
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8584..9294
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(8729..8747)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     polyA_signal    9295..9342
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        9373..9621
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     9373..9393
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     9544..9563
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(9775..9793)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9861..9965
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9966..10034,10035..10826)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10184..10203)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10997..11585
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11486..11505
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agcaacgcgg cctttttctt aagcgtgatg cggttttggc agtacatcaa tgggcgtgga
       61 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
      121 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
      181 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
      241 cgtcagatct ctagagccgc caccatgcac catcaccatc accattccgg cgattacaaa
      301 gacgatgacg ataagatggc gcctaagaag aaacgcaaag tgcggggcat gaagaaagaa
      361 aagattgatc tgttttacgg agccctgctg cacgacatcg gaaaggtcat ccagcgagca
      421 accggagagc ggaagaaaca cgcacttgtg ggcgccgact ggttcgacga gatcgccgac
      481 aaccaagtca tctcggatca gatccggtac catatggcca actaccagtc tgataagctc
      541 ggcaacgatc acctggctta catcacctac attgccgaca acatcgcctc cggtgtcgac
      601 cgccggcaat ccaacgaaga gtcagacgaa gatacctccg caaagatctg ggacacctac
      661 acgaaccagg ccgacatctt taacgtgttc ggagcgcaga ccgataagcg gtacttcaag
      721 cctaccgtgc tgaatctcaa gtcgaagccc aacttcgcgt ccgccactta cgaacccttt
      781 agcaagggcg attacgctgc catcgccacc cggattaaga acgaactggc cgagttcgag
      841 ttcaaccaag tccagattga ctccctgctc aaccttttcg aggctactct ctccttcgtg
      901 ccgtcaagca ccaacactaa ggaaatcgcc gacatctccc tggccgacca ttcccgcttg
      961 actgctgcct tcgctctggc gatctacgac tacctggagg acaagggtcg gcacaactac
     1021 aaagaggacc tgttcaccaa agtgtcagcg ttctatgaag aagaagcctt cctgctggcc
     1081 tccttcgacc tgtcgggaat ccaggacttt atctacaaca ttaacatcgc aactaacggc
     1141 gcggcgaagc agctgaaggc ccggagcctc tacctggact ttatgtccga gtacatcgcc
     1201 gatagcctgc tggacaagct gggactgaac agggctaaca tgctttacgt cggcggcgga
     1261 cacgcctact tcgtcctggc caacaccgaa aagactgtgg aaaccctggt gcagtttgag
     1321 aaggatttca accagttcct gttggcaaac ttccagaccc gcctctatgt ggcctttggc
     1381 tggggttcct tcgcggccaa ggacatcatg tccgagctga atagccccga gtcctaccgc
     1441 caagtgtacc aaaaggcttc gcgcatgatc tccaaaaaga aaatctccag atacgactac
     1501 cagacactga tgctcctgaa tcgcggtgga aagtcctcag agagagagtg cgagatttgc
     1561 cactccgtgg agaacctggt gtcctaccac gaccagaaag tctgtgacat ttgccgggga
     1621 ctgtaccagt tctcgaaaga aattgcccat gaccacttca tcattaccga aaatgagggg
     1681 ctgccgattg gaccaaacgc gtgcttaaag ggcgtggcat tcgaaaagct gtcccaagaa
     1741 gcgttcagcc gggtctacgt gaagaatgac tataaggccg gtaccgtgaa ggctacgcat
     1801 gtgttcgtgg gggattacca gtgcgacgag atctacaact acgccgccct gagcaagaac
     1861 gagaacggcc taggcatcaa gagactggcc gtggtccggc tcgacgtgga tgacttgggc
     1921 gccgccttca tggccggttt cagccagcag ggaaacggac aatactccac tctgtcaaga
     1981 tcggccacat tctcccggag catgtcgctg ttcttcaaag tgtacattaa ccagttcgcc
     2041 tccgacaaga agctgagcat tatctacgcg ggcggcgatg acgtgttcgc cattggatcg
     2101 tggcaggata tcatcgcgtt cactgtggaa cttcgcgaaa acttcatcaa gtggaccaac
     2161 gggaagctca ccctctccgc ggggataggg ttgttcgccg acaagactcc tattagcctg
     2221 atggctcacc agaccgggga actggaagag gccgccaagg gcaacgaaaa ggactccatc
     2281 tcgctgttct caagcgacta cactttcaag tttgataggt tcatcactaa cgtgtacgac
     2341 gacaaactgg aacagattag atacttcttc aaccatcaag acgagagggg aaagaacttc
     2401 atctataagc ttattgagct tttgaggaac cacgaccgca tgaatatggc acgcctcgcc
     2461 tattacctca ctcgcctgga agaactgacc cgggagactg acagggacaa gttcaagacc
     2521 ttcaagaacc tgttctactc ctggtacacc aacaagaacg ataaggaccg gaaggaagcc
     2581 gagctcgcgc tcctgctgta catctacgaa atcagaaagg attaacggca ataaaaagac
     2641 agaataaaac gcacggtgtt gggtcgtttg ttcgcacaca ttagctagcc gtcagcacac
     2701 attgtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
     2761 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
     2821 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
     2881 cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatctc tagagccgcc
     2941 accatggatt acaaagacga tgacgataag atggcgccta agaagaaacg caaagtgcgg
     3001 ggcatgacca tcctgaccga cgagaactac gtggacatcg ccgagaaagc catcctgaag
     3061 ctggaaagaa acaccagaaa tagaaagaac cctgatgcct tcttcctgac cacatctaag
     3121 ctgcggaacc tgctgagcct gacaagcacc ctgttcgacg agagcaaggt gaaggaatac
     3181 gacgccctgc tggacagaat cgcttatctg agagtgcagt tcgtgtacca ggccggcaga
     3241 gagatcgccg tgaaagatct gatcgagaag gcccagatcc tggaagctct gaaagagatc
     3301 aaggaccggg aaaccctgca gagattctgc agatacatgg aagccctggt ggcctacttc
     3361 aagttctacg gcggcaagga ctgacggcaa taaaaagaca gaataaaacg cacggtgttg
     3421 ggtcgtttgt tcagttcttt tgccttactt tcaatgcatg cggtgatgcg gttttggcag
     3481 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
     3541 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
     3601 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
     3661 agagctcgtt tagtgaaccg tcagatctct agagccgcca ccatggatta caaagacgat
     3721 gacgataaga tggcgcctaa gaagaaacgc aaagtgcggg gcatgacctt cgccaagatc
     3781 aaattcagcg cccagatccg gctggaaacc ggcctgcaca tcggaggatc tgatgccttt
     3841 gccgctatcg gcgccatcga cagccctgtg atcaaggacc ccatcaccaa cctgcctatc
     3901 atccccggct ctagcctgaa gggcaagatg agaacactgc tggccaaggt gtacaacgaa
     3961 aaggtggccg agaagcctag cgacgacagc gacatcctga gcagactgtt cggaaatagc
     4021 aaggataagc ggttcaagat gggcagactg atcttccggg acgccttcct gagcaacgcc
     4081 gacgagctgg attctctggg cgtgcggagc tacaccgagg tgaagttcga gaacaccatc
     4141 gatagaatca ccgccgaggc caatcctaga cagatcgaga gagccattcg gaactcaaca
     4201 ttcgacttcg agctgatcta cgagatcact gatgagaatg agaaccaggt cgaggaagat
     4261 ttcaaggtga tcagagacgg cctgaagctg ctggaactgg actacctggg cggaagcggc
     4321 tccagaggct acggcaaagt ggcttttgag aacctgaaag ccaccacagt gttcggcaac
     4381 tacgacgtga aaaccctgaa cgagctgctg accgccgaag tgtgacggca ataaaaagac
     4441 agaataaaac gcacggtgtt gggtcgtttg ttctggattg cgagaatgga ctagtagcaa
     4501 actgtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
     4561 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
     4621 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
     4681 cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatctc tagagccgcc
     4741 accatggatt acaaagacga tgacgataag atggcgccta agaagaaacg caaagtgcgg
     4801 ggcatgactt acaagctcta cattatgacc tttcaaaacg cccacttcgg ttccggcact
     4861 ctggactcat cgaagctgac cttctccgcg gatagaatct tctcggcact cgtgctcgag
     4921 gctctgaaga tgggaaagct cgacgccttc ttggccgagg ccaaccagga taagttcact
     4981 ctgaccgacg cgttcccatt ccaattcggt cctttcctgc cgaaaccgat tggttacccc
     5041 aagcacgacc agatcgacca gtctgtggac gtgaaggaag tccgccgcca agcgaagctg
     5101 tccaaaaagc tccagttcct ggctctggaa aacgtcgacg actacctgaa cggagagctg
     5161 tttgagaatg aggaacacgc cgtgatcgac acagtgacca agaaccagcc ccataaagat
     5221 gataatctgt accaagtggc caccactcgg ttctcgaacg acacctccct ttacgtgatc
     5281 gccaacgaat ccgatctgct gaacgaactg atgagcagcc ttcagtactc cgggctgggc
     5341 ggcaaaaggt cctcaggatt cggcagattt gagctggaca tccagaacat tcccttggaa
     5401 ctgtccgacc ggctgacgaa gaaccacagc gacaaggtca tgtcacttac caccgccctc
     5461 ccggtggacg ctgatctcga ggaagcgatg gaagatggcc attacctgtt gaccaagtcg
     5521 tccggattcg cattctccca cgccaccaac gaaaactatc ggaagcagga cctgtacaag
     5581 ttcgcctccg ggagcacctt cagcaagact ttcgagggac agatcgtgga cgtgcgccct
     5641 ctcgatttcc ctcacgccgt gctgaactac gccaagccgc tgttctttaa gctcgaagtc
     5701 taacggcaat aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt cgcacattca
     5761 aaaacaggca attggacaag cgtgatgcgg ttttggcagt acatcaatgg gcgtggatag
     5821 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
     5881 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
     5941 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcgttt agtgaaccgt
     6001 cagatctcta gagccgccac catggattac aaagacgatg acgataagat ggcgcctaag
     6061 aagaaacgca aagtgcgggg catgaaaaat gactaccgga ccttcaagct gagcctgctg
     6121 accctggctc ctatccacat cggcaacggc gagaagtaca ccagcagaga attcatctac
     6181 gagaacaaga agttctactt ccccgacatg ggcaagttct acaacaagat ggtggaaaag
     6241 agactggccg agaagttcga ggccttcctg atccagacca gacccaacgc cagaaacaac
     6301 cggctgattt cttttctgaa cgacaacaga atcgccgaaa gatcttttgg cggctacagc
     6361 atcagtgaaa ccggcctgga atctgataag aaccctaaca gcgccggagc tatcaacgag
     6421 gtgaacaaat tcatccggga cgccttcgga aatccttaca tcccaggcag cagcctgaag
     6481 ggcgccatcc gcaccatcct gatgaacacc acacctaagt ggaacaacga gaacgccgtg
     6541 aacgacttcg gcagattccc aaaggaaaac aagaacctga tcccttgggg acctaagaaa
     6601 ggcaaggaat acgacgacct gttcaacgcc atcagagtgt ccgacagcaa gcccttcgac
     6661 aacaaaagcc tgatcctcgt gcagaagtgg gactacagcg ccaaaaccaa caaggccaag
     6721 cctctgcctc tgtacagaga gtctatcagc cctctgacca agatcgagtt cgagataaca
     6781 acaaccactg atgaggccgg cagactgatc gaggaactgg gaaagcgggc ccaggccttt
     6841 tataaggact acaaggcctt tttcctgtct gaattccctg atgataagat ccaggctaat
     6901 ctgcaatacc ccatctacct gggcgccggc agcggcgctt ggacaaagac cctgtttaag
     6961 caggccgacg gcatcctgca gcggagatac tccagaatga aaaccaagat ggtcaagaag
     7021 ggcgtgctga agctgacaaa ggcccctctg aaaacagtga agatccccag cggcaaccac
     7081 agcctggtga agaatcacga gagcttctac gagatgggca aagccaactt catgatcaag
     7141 gaaatcgaca agtgacggca ataaaaagac agaataaaac gcacggtgtt gggtcgtttg
     7201 ttcatgtgtg ccggccggag aataaaagtc taagtgatgc ggttttggca gtacatcaat
     7261 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
     7321 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
     7381 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt
     7441 ttagtgaacc gtcagatctc tagagccgcc accatggatt acaaagacga tgacgataag
     7501 atggcgccta agaagaaacg caaagtgcgg ggcatgaaaa agctcgtgtt cacctttaag
     7561 cggatcgacc accctgctca ggacctggcc gtgaaattcc acggcttcct gatggaacag
     7621 ctggatagcg actacgtgga ctacctgcac cagcagcaga ccaaccccta cgccacaaag
     7681 gtgatccagg gcaaagagaa cacccagtgg gtcgtgcatc tgctgacaga cgacatcgag
     7741 gacaaggtgt tcatgaccct gctgcagatc aaggaagtgt ccctgaacga cctgcctaag
     7801 ttgtctgtgg aaaaggtgga aatccaggag ctgggcgctg ataagctgct cgagatcttc
     7861 aacagcgagg aaaaccagac ctacttcagc atcatcttcg agacacctac aggctttaaa
     7921 agccagggca gctacgtgat cttccccagc atgcggctga tctttcagag cctgatgcag
     7981 aagtacggca gactggtgga aaaccagcct gagatcgagg aagataccct ggactacctg
     8041 agcgagcaca gcaccatcac caattacaga ctggaaacaa gctacttcag agtgcataga
     8101 cagagaatcc ccgccttccg gggcaagctg accttcaagg tgcagggagc ccagacactg
     8161 aaggcctacg tgaagatgct gctgaccttc ggcgagtaca gcggcctggg catgaaaacc
     8221 agcctgggaa tgggcggcat caagctggaa gaaagaaagg actgacggca ataaaaagac
     8281 agaataaaac gcacggtgtt gggtcgtttg ttcgtgcgat agagggatcc cgcattgaat
     8341 tatgtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
     8401 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
     8461 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
     8521 cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatctc tagagccgcc
     8581 accatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc
     8641 aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag
     8701 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg
     8761 cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag
     8821 caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag
     8881 cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag
     8941 gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc
     9001 gtaatgcaga agaaaaccat gggctgggag gcctcctccg agcggatgta ccccgaggac
     9061 ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac
     9121 gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac
     9181 gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac
     9241 gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa gtaacggcaa
     9301 taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt tcgagcagat tgtactgaga
     9361 gtgcaccggt tggagggcct atttcccatg attccttcat atttgcatat acgatacaag
     9421 gctgttagag agataattag aattaatttg actgtaaaca caaagatatt agtacaaaat
     9481 acgtgacgta gaaagtaata atttcttggg tagtttgcag ttttaaaatt atgttttaaa
     9541 atggactatc atatgcttac cgtaacttga aagtatttcg atttcttggc tttatatatc
     9601 ttgtggaaag gacgaaacac cgatataaac ctaattacct cgagagggga cggaaacccg
     9661 tcttcgatga agcgattcag aagacttgat ataaacctaa ttacctcgag aggggacttt
     9721 tttacatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
     9781 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     9841 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     9901 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     9961 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
    10021 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
    10081 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
    10141 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
    10201 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
    10261 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
    10321 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
    10381 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
    10441 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
    10501 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
    10561 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
    10621 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
    10681 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
    10741 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
    10801 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
    10861 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
    10921 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
    10981 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
    11041 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
    11101 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
    11161 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
    11221 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
    11281 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
    11341 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
    11401 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
    11461 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
    11521 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
    11581 ggaaaaacgc c
//
