LOCUS       sequence-382947-       11600 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of Csm complex from separate promoters; GFP 
            backbone (Used for RNA knockdown).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11600)
  AUTHORS   Colognori D, Trinidad M, Doudna JA
  TITLE     Precise transcript targeting by CRISPR-Csm complexes.
  JOURNAL   Nat Biotechnol. 2023 Jan 23. doi: 10.1038/s41587-022-01649-9.
  PUBMED    36690762
REFERENCE   2  (bases 1 to 11600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2023 Jan 23. doi: 10.1038/s41587-022-01649-9."
COMMENT     Sequence Label: pDAC446
FEATURES             Location/Qualifiers
     source          1..11600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        9..212
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             252..269
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             276..299
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             306..326
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2610..2657
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        2688..2891
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      2922..2931
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2931..2954
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2961..2981
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    3369..3416
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        3447..3650
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      3681..3690
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3690..3713
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3720..3740
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    4410..4457
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        4488..4691
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      4722..4731
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4731..4754
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4761..4781
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5688..5735
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        5766..5969
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      6000..6009
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6009..6032
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             6039..6059
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7140..7187
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        7218..7421
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      7452..7461
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7461..7484
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             7491..7511
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8250..8297
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        8328..8531
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      8562..8571
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(8568..8570,8571..8573,8574..9287)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(8568..8570,8571..8573,8574..9284)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8613..8634)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8874..8893)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9221..9242
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    9288..9335
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        9366..9614
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     9366..9386
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     9537..9556
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(9768..9786)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9854..9958
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9959..10027,10028..10819)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10177..10196)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10990..11578
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11479..11498
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcttaagcgt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
       61 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      121 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      181 gtgtacggtg ggaggtctat ataagcagag ctcgtttagt gaaccgtcag atctctagag
      241 ccgccaccat gcaccatcac catcaccatt ccggcgatta caaagacgat gacgataaga
      301 tggcgcctaa gaagaaacgc aaagtgcggg gcatgaagaa agaaaagatt gatctgtttt
      361 acggagccct gctgcacgac atcggaaagg tcatccagcg agcaaccgga gagcggaaga
      421 aacacgcact tgtgggcgcc gactggttcg acgagatcgc cgacaaccaa gtcatctcgg
      481 atcagatccg gtaccatatg gccaactacc agtctgataa gctcggcaac gatcacctgg
      541 cttacatcac ctacattgcc gacaacatcg cctccggtgt cgaccgccgg caatccaacg
      601 aagagtcaga cgaagatacc tccgcaaaga tctgggacac ctacacgaac caggccgaca
      661 tctttaacgt gttcggagcg cagaccgata agcggtactt caagcctacc gtgctgaatc
      721 tcaagtcgaa gcccaacttc gcgtccgcca cttacgaacc ctttagcaag ggcgattacg
      781 ctgccatcgc cacccggatt aagaacgaac tggccgagtt cgagttcaac caagtccaga
      841 ttgactccct gctcaacctt ttcgaggcta ctctctcctt cgtgccgtca agcaccaaca
      901 ctaaggaaat cgccgacatc tccctggccg accattcccg cttgactgct gccttcgctc
      961 tggcgatcta cgactacctg gaggacaagg gtcggcacaa ctacaaagag gacctgttca
     1021 ccaaagtgtc agcgttctat gaagaagaag ccttcctgct ggcctccttc gacctgtcgg
     1081 gaatccagga ctttatctac aacattaaca tcgcaactaa cggcgcggcg aagcagctga
     1141 aggcccggag cctctacctg gactttatgt ccgagtacat cgccgatagc ctgctggaca
     1201 agctgggact gaacagggct aacatgcttt acgtcggcgg cggacacgcc tacttcgtcc
     1261 tggccaacac cgaaaagact gtggaaaccc tggtgcagtt tgagaaggat ttcaaccagt
     1321 tcctgttggc aaacttccag acccgcctct atgtggcctt tggctggggt tccttcgcgg
     1381 ccaaggacat catgtccgag ctgaatagcc ccgagtccta ccgccaagtg taccaaaagg
     1441 cttcgcgcat gatctccaaa aagaaaatct ccagatacga ctaccagaca ctgatgctcc
     1501 tgaatcgcgg tggaaagtcc tcagagagag agtgcgagat ttgccactcc gtggagaacc
     1561 tggtgtccta ccacgaccag aaagtctgtg acatttgccg gggactgtac cagttctcga
     1621 aagaaattgc ccatgaccac ttcatcatta ccgaaaatga ggggctgccg attggaccaa
     1681 acgcgtgctt aaagggcgtg gcattcgaaa agctgtccca agaagcgttc agccgggtct
     1741 acgtgaagaa tgactataag gccggtaccg tgaaggctac gcatgtgttc gtgggggatt
     1801 accagtgcga cgagatctac aactacgccg ccctgagcaa gaacgagaac ggcctaggca
     1861 tcaagagact ggccgtggtc cggctcgacg tggatgactt gggcgccgcc ttcatggccg
     1921 gtttcagcca gcagggaaac ggacaatact ccactctgtc aagatcggcc acattctccc
     1981 ggagcatgtc gctgttcttc aaagtgtaca ttaaccagtt cgcctccgac aagaagctga
     2041 gcattatcta cgcgggcggc gatgacgtgt tcgccattgg atcgtggcag gatatcatcg
     2101 cgttcactgt ggaacttcgc gaaaacttca tcaagtggac caacgggaag ctcaccctct
     2161 ccgcggggat agggttgttc gccgacaaga ctcctattag cctgatggct caccagaccg
     2221 gggaactgga agaggccgcc aagggcaacg aaaaggactc catctcgctg ttctcaagcg
     2281 actacacttt caagtttgat aggttcatca ctaacgtgta cgacgacaaa ctggaacaga
     2341 ttagatactt cttcaaccat caagacgaga ggggaaagaa cttcatctat aagcttattg
     2401 agcttttgag gaaccacgac cgcatgaata tggcacgcct cgcctattac ctcactcgcc
     2461 tggaagaact gacccgggag actgacaggg acaagttcaa gaccttcaag aacctgttct
     2521 actcctggta caccaacaag aacgataagg accggaagga agccgagctc gcgctcctgc
     2581 tgtacatcta cgaaatcaga aaggattaac ggcaataaaa agacagaata aaacgcacgg
     2641 tgttgggtcg tttgttcgca cacattagct agccgtcagc acacattgtg atgcggtttt
     2701 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     2761 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     2821 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     2881 taagcagagc tcgtttagtg aaccgtcaga tctctagagc cgccaccatg gattacaaag
     2941 acgatgacga taagatggcg cctaagaaga aacgcaaagt gcggggcatg accatcctga
     3001 ccgacgagaa ctacgtggac atcgccgaga aagccatcct gaagctggaa agaaacacca
     3061 gaaatagaaa gaaccctgat gccttcttcc tgaccacatc taagctgcgg aacctgctga
     3121 gcctgacaag caccctgttc gacgagagca aggtgaagga atacgacgcc ctgctggaca
     3181 gaatcgctta tctgagagtg cagttcgtgt accaggccgg cagagagatc gccgtgaaag
     3241 atctgatcga gaaggcccag atcctggaag ctctgaaaga gatcaaggac cgggaaaccc
     3301 tgcagagatt ctgcagatac atggaagccc tggtggccta cttcaagttc tacggcggca
     3361 aggactgacg gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcagtt
     3421 cttttgcctt actttcaatg catgcggtga tgcggttttg gcagtacatc aatgggcgtg
     3481 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
     3541 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
     3601 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
     3661 accgtcagat ctctagagcc gccaccatgg attacaaaga cgatgacgat aagatggcgc
     3721 ctaagaagaa acgcaaagtg cggggcatga ccttcgccaa gatcaaattc agcgcccaga
     3781 tccggctgga aaccggcctg cacatcggag gatctgatgc ctttgccgct atcggcgcca
     3841 tcgacagccc tgtgatcaag gaccccatca ccaacctgcc tatcatcccc ggctctagcc
     3901 tgaagggcaa gatgagaaca ctgctggcca aggtgtacaa cgaaaaggtg gccgagaagc
     3961 ctagcgacga cagcgacatc ctgagcagac tgttcggaaa tagcaaggat aagcggttca
     4021 agatgggcag actgatcttc cgggacgcct tcctgagcaa cgccgacgag ctggattctc
     4081 tgggcgtgcg gagctacacc gaggtgaagt tcgagaacac catcgataga atcaccgccg
     4141 aggccaatcc tagacagatc gagagagcca ttcggaactc aacattcgac ttcgagctga
     4201 tctacgagat cactgatgag aatgagaacc aggtcgagga agatttcaag gtgatcagag
     4261 acggcctgaa gctgctggaa ctggactacc tgggcggaag cggctccaga ggctacggca
     4321 aagtggcttt tgagaacctg aaagccacca cagtgttcgg caactacgac gtgaaaaccc
     4381 tgaacgagct gctgaccgcc gaagtgtgac ggcaataaaa agacagaata aaacgcacgg
     4441 tgttgggtcg tttgttctgg attgcgagaa tggactagta gcaaactgtg atgcggtttt
     4501 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     4561 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     4621 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     4681 taagcagagc tcgtttagtg aaccgtcaga tctctagagc cgccaccatg gattacaaag
     4741 acgatgacga taagatggcg cctaagaaga aacgcaaagt gcggggcatg acttacaagc
     4801 tctacattat gacctttcaa aacgcccact tcggttccgg cactctggac tcatcgaagc
     4861 tgaccttctc cgcggataga atcttctcgg cactcgtgct cgaggctctg aagatgggaa
     4921 agctcgacgc cttcttggcc gaggccaacc aggataagtt cactctgacc gacgcgttcc
     4981 cattccaatt cggtcctttc ctgccgaaac cgattggtta ccccaagcac gaccagatcg
     5041 accagtctgt ggacgtgaag gaagtccgcc gccaagcgaa gctgtccaaa aagctccagt
     5101 tcctggctct ggaaaacgtc gacgactacc tgaacggaga gctgtttgag aatgaggaac
     5161 acgccgtgat cgacacagtg accaagaacc agccccataa agatgataat ctgtaccaag
     5221 tggccaccac tcggttctcg aacgacacct ccctttacgt gatcgccaac gaatccgatc
     5281 tgctgaacga actgatgagc agccttcagt actccgggct gggcggcaaa aggtcctcag
     5341 gattcggcag atttgagctg gacatccaga acattccctt ggaactgtcc gaccggctga
     5401 cgaagaacca cagcgacaag gtcatgtcac ttaccaccgc cctcccggtg gacgctgatc
     5461 tcgaggaagc gatggaagat ggccattacc tgttgaccaa gtcgtccgga ttcgcattct
     5521 cccacgccac caacgaaaac tatcggaagc aggacctgta caagttcgcc tccgggagca
     5581 ccttcagcaa gactttcgag ggacagatcg tggacgtgcg ccctctcgat ttccctcacg
     5641 ccgtgctgaa ctacgccaag ccgctgttct ttaagctcga agtctaacgg caataaaaag
     5701 acagaataaa acgcacggtg ttgggtcgtt tgttcgcaca ttcaaaaaca ggcaattgga
     5761 caagcgtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg
     5821 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
     5881 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
     5941 tacggtggga ggtctatata agcagagctc gtttagtgaa ccgtcagatc tctagagccg
     6001 ccaccatgga ttacaaagac gatgacgata agatggcgcc taagaagaaa cgcaaagtgc
     6061 ggggcatgaa aaatgactac cggaccttca agctgagcct gctgaccctg gctcctatcc
     6121 acatcggcaa cggcgagaag tacaccagca gagaattcat ctacgagaac aagaagttct
     6181 acttccccga catgggcaag ttctacaaca agatggtgga aaagagactg gccgagaagt
     6241 tcgaggcctt cctgatccag accagaccca acgccagaaa caaccggctg atttcttttc
     6301 tgaacgacaa cagaatcgcc gaaagatctt ttggcggcta cagcatcagt gaaaccggcc
     6361 tggaatctga taagaaccct aacagcgccg gagctatcaa cgaggtgaac aaattcatcc
     6421 gggacgcctt cggaaatcct tacatcccag gcagcagcct gaagggcgcc atccgcacca
     6481 tcctgatgaa caccacacct aagtggaaca acgagaacgc cgtgaacgac ttcggcagat
     6541 tcccaaagga aaacaagaac ctgatccctt ggggacctaa gaaaggcaag gaatacgacg
     6601 acctgttcaa cgccatcaga gtgtccgaca gcaagccctt cgacaacaaa agcctgatcc
     6661 tcgtgcagaa gtgggactac agcgccaaaa ccaacaaggc caagcctctg cctctgtaca
     6721 gagagtctat cagccctctg accaagatcg agttcgagat aacaacaacc actgatgagg
     6781 ccggcagact gatcgaggaa ctgggaaagc gggcccaggc cttttataag gactacaagg
     6841 cctttttcct gtctgaattc cctgatgata agatccaggc taatctgcaa taccccatct
     6901 acctgggcgc cggcagcggc gcttggacaa agaccctgtt taagcaggcc gacggcatcc
     6961 tgcagcggag atactccaga atgaaaacca agatggtcaa gaagggcgtg ctgaagctga
     7021 caaaggcccc tctgaaaaca gtgaagatcc ccagcggcaa ccacagcctg gtgaagaatc
     7081 acgagagctt ctacgagatg ggcaaagcca acttcatgat caaggaaatc gacaagtgac
     7141 ggcaataaaa agacagaata aaacgcacgg tgttgggtcg tttgttcatg tgtgccggcc
     7201 ggagaataaa agtctaagtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     7261 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     7321 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     7381 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga
     7441 tctctagagc cgccaccatg gattacaaag acgatgacga taagatggcg cctaagaaga
     7501 aacgcaaagt gcggggcatg aaaaagctcg tgttcacctt taagcggatc gaccaccctg
     7561 ctcaggacct ggccgtgaaa ttccacggct tcctgatgga acagctggat agcgactacg
     7621 tggactacct gcaccagcag cagaccaacc cctacgccac aaaggtgatc cagggcaaag
     7681 agaacaccca gtgggtcgtg catctgctga cagacgacat cgaggacaag gtgttcatga
     7741 ccctgctgca gatcaaggaa gtgtccctga acgacctgcc taagttgtct gtggaaaagg
     7801 tggaaatcca ggagctgggc gctgataagc tgctcgagat cttcaacagc gaggaaaacc
     7861 agacctactt cagcatcatc ttcgagacac ctacaggctt taaaagccag ggcagctacg
     7921 tgatcttccc cagcatgcgg ctgatctttc agagcctgat gcagaagtac ggcagactgg
     7981 tggaaaacca gcctgagatc gaggaagata ccctggacta cctgagcgag cacagcacca
     8041 tcaccaatta cagactggaa acaagctact tcagagtgca tagacagaga atccccgcct
     8101 tccggggcaa gctgaccttc aaggtgcagg gagcccagac actgaaggcc tacgtgaaga
     8161 tgctgctgac cttcggcgag tacagcggcc tgggcatgaa aaccagcctg ggaatgggcg
     8221 gcatcaagct ggaagaaaga aaggactgac ggcaataaaa agacagaata aaacgcacgg
     8281 tgttgggtcg tttgttcgtg cgatagaggg atcccgcatt gaattatgtg atgcggtttt
     8341 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     8401 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     8461 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     8521 taagcagagc tcgtttagtg aaccgtcaga tctctagagc cgccaccatg gtgagcaagg
     8581 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     8641 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     8701 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
     8761 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
     8821 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     8881 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     8941 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     9001 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
     9061 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
     9121 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
     9181 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     9241 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtgacgg caataaaaag
     9301 acagaataaa acgcacggtg ttgggtcgtt tgttcgtaga tggcgcgcct ttgttgaccc
     9361 ggttggaggg cctatttccc atgattcctt catatttgca tatacgatac aaggctgtta
     9421 gagagataat tagaattaat ttgactgtaa acacaaagat attagtacaa aatacgtgac
     9481 gtagaaagta ataatttctt gggtagtttg cagttttaaa attatgtttt aaaatggact
     9541 atcatatgct taccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
     9601 aaggacgaaa caccgatata aacctaatta cctcgagagg ggacggaaac ccgtcttcga
     9661 tgaagcgatt cagaagactt gatataaacc taattacctc gagaggggac ttttttacat
     9721 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg
     9781 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     9841 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     9901 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     9961 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
    10021 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
    10081 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
    10141 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
    10201 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
    10261 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
    10321 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
    10381 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
    10441 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
    10501 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
    10561 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
    10621 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
    10681 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
    10741 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
    10801 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
    10861 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
    10921 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
    10981 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
    11041 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
    11101 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
    11161 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
    11221 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
    11281 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
    11341 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
    11401 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
    11461 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
    11521 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
    11581 cgccagcaac gcggcctttt
//
