LOCUS       sequence-382962-        9445 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector to introduce osTir1 in cells. Hygromycin selection 
            (2A-fusion). Auxin-inducible degron system..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9445)
  AUTHORS   Valton AL, Venev SV, Mair B, Khokhar ES, Tong AHY, Usaj M, Chan K, 
            Pai AA, Moffat J, Dekker J
  TITLE     A cohesin traffic pattern genetically linked to gene regulation.
  JOURNAL   Nat Struct Mol Biol. 2022 Dec 8. doi: 10.1038/s41594-022-00890-9.
  PUBMED    36482254
REFERENCE   2  (bases 1 to 9445)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Struct 
            Mol Biol. 2022 Dec 8. doi: 10.1038/s41594-022-00890-9."
COMMENT     Sequence Label: pEN396-pCAGGS-TIR1-V5-2A-HygroR
FEATURES             Location/Qualifiers
     source          1..9445
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(201..269,270..1061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(419..438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1721..1740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1974..1991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2144..2149,2150..2167,2168..2174)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2187..2209
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2206..2222
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2206..2222
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        3243..3622
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          3900..4916
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     4840..4862
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     4924..4943
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             4995..6719
                     /codon_start=1
                     /product="rice transport inhibitor response 1 protein"
                     /label=OsTIR1
                     /note="F-box protein that mediates auxin-dependent 
                     interaction of a ubiquitin ligase with an auxin-inducible 
                     degron (Tan et al., 2007)"
                     /translation="MTYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFV
                     GNCYAVRAGRVAARFPNVRALTVKGKPHFADFNLVPPDWGGYAGPWIEAAARGCHGLEE
                     LRMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEV
                     EDRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTL
                     AKILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYP
                     LCAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELR
                     VFPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRL
                     CILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSI
                     AFAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKG
                     CQVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL"
     CDS             6720..6761
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6771..6824
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6825..7847
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             6825..7847
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     complement(7875..7892)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7881..8088
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(9051..9068)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9051..9067)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9060..9082)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(9276..9295)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9395..9417
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     2221 accatgatta cgccatttaa atgaaaagtg gagaggcgtg agtcggagaa ctaagaagaa
     2281 acgcctcctc cagggctgag ctccgcccct aggctggcgt gctgcgtact cccccagtac
     2341 tcttgggggt gaggcgtttg tcagaaggag gcgctcagct actttataca cccggaggag
     2401 ggctggctcc gcccccagga gggacggtta ctagcagaat gcagacggtg ctctgcatct
     2461 ttttgtcaag ggacggtgtt ctgatccacg aaaccctccc tcggagagtt cgggcccctc
     2521 cgggccagtg tcctgcaaac aacctctcct gagcttgagg aaagacaaga accgtttcca
     2581 aacccaggtg attcggccac gcccccaagt taaggttccg ccctgtacgt tcccaggtaa
     2641 gctccgcctt gatcccgtcc cactggtaag aaaaggccct gtgataggtg tgctctggtt
     2701 tgcaggctcg cacccagctg gggccccttc ccatgctgct ctgggataaa ggcttttcaa
     2761 gccgcgctcc agagcttggc cttcacaaag aacttcaagg cagtaacttt aattctttag
     2821 cttctccact aggacctctc cttttcacta gttaactctc aggtccccca gtgaactcac
     2881 agtggccagt cacttgttca cttttgctgg gcctcttaga actctaattt acgtaccccc
     2941 tcctgggcct ccgcaagact cacaaaagac tcaacccttt cccaagtcat ccctcccatc
     3001 taatggtgca agcacacaat ctcaggcagc caggacccac aaagtgaaga gtaaaaacgc
     3061 actctgctgg gcagtggtgg cacacacctt taatcccgat gctcaggagg cagaggctgg
     3121 cagtccttga gttccaggcc agcctggtct acagagtgag ttctaggaca gccaggacta
     3181 cactgaaaaa ccctatctca aaacaaacag aaaacatccc aaaatttaaa tggcgcgccg
     3241 tcgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
     3301 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
     3361 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
     3421 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
     3481 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
     3541 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
     3601 attagtcatc gctattacca tggtcgaggt gagccccacg ttctgcttca ctctccccat
     3661 ctcccccccc tccccacccc caattttgta tttatttatt ttttaattat tttgtgcagc
     3721 gatgggggcg gggggggggg gggcgcgcgc caggcggggs ggggsggggs grggggsggg
     3781 gsggggsgrg gsggrgrggk gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc
     3841 cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg
     3901 gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc
     3961 cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc
     4021 cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa
     4081 gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg
     4141 tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct
     4201 gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg
     4261 gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt
     4321 gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc
     4381 cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt acggggcgtg
     4441 gcgcggggct cgccgtgccg ggcggggggt ggcggcaggk gggggtgccg ggcggggcgg
     4501 ggccgccycg ggccggggrg ggctcggggg rggggcgcgg cggcccccgg agcgccggcg
     4561 gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc
     4621 agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc
     4681 cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg
     4741 gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc
     4801 ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga
     4861 ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc
     4921 tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattc ctcgagaatt
     4981 ctcacgcgtc caccatgaca tacttccctg aggaggtggt cgaacatatt ttctcattcc
     5041 ttcccgccca acgagatagg aataccgtgt ccctggtgtg caaggtctgg tatgagatcg
     5101 aacgcttgag ccgcagaggt gtttttgtgg gtaattgtta tgccgtacga gccgggcgag
     5161 tggccgcccg gttcccgaac gtcagggccc ttacagtgaa gggcaagcct cactttgccg
     5221 acttcaattt ggtgcctccg gactggggcg gctacgccgg gccctggatc gaggccgctg
     5281 cgcgcgggtg tcatggtctg gaagaactca ggatgaaaag gatggtggtg tcagacgagt
     5341 cactggaact gcttgcccgc agcttccccc gattccgcgc cctggtgctg atcagctgtg
     5401 aaggatttag caccgacggt ctggctgctg tagctagcca ttgtaaactg ctgcgagaac
     5461 tggacctgca ggaaaatgag gtagaggata ggggacctcg gtggctgagc tgtttcccgg
     5521 atagctgcac tagcctggtg agcttgaatt tcgcttgtat caagggcgaa gtgaatgccg
     5581 ggagtttgga gagactcgtt tcccgatccc ccaacctgag atctctgcgg ctgaacagat
     5641 ccgtttctgt ggacacgctc gccaagattt tgctccggac tcccaacctg gaggatctgg
     5701 ggaccggaaa tctgaccgat gatttccaga cagagagcta ctttaagctc actagtgctc
     5761 tggaaaaatg caaaatgctt aggtcactga gcgggttttg ggacgctagc cctgtctgcc
     5821 tgtcttttat ctatcccctg tgtgcacagt tgaccggact taatctgagt tacgctccaa
     5881 ccctcgacgc tagtgacctt actaagatga tcagccgctg cgtgaagctc caaaggctct
     5941 gggtactgga ttgtatttca gacaagggac tgcaagttgt ggcctcctcc tgcaaagatc
     6001 tgcaggagct gagggtgttc ccttcagatt tctatgtggc cggatacagc gcagtcaccg
     6061 aggagggatt ggtagcagtg tccctggggt gccccaaact caatagtttg ctgtacttct
     6121 gtcatcagat gaccaatgct gcccttgtta ccgttgccaa aaactgtcca aacttcacaa
     6181 ggtttagact gtgcatcttg gagcccggta agcccgacgt cgtaacgtca cagccactgg
     6241 acgaagggtt cggtgccata gtacgcgaat gtaagggtct ccagcggctg agcatatccg
     6301 gcctgctgac tgacaaagtg ttcatgtata ttgggaagta cgccaagcag ctggaaatgc
     6361 tgtcaatcgc attcgccgga gattcagata aagggatgat gcacgtgatg aacgggtgca
     6421 agaacctgag aaaactcgaa attcgcgatt ccccttttgg ggacgccgca ctgctgggca
     6481 atttcgcccg gtacgagacg atgaggtcac tttggatgtc aagctgcaac gtgaccctca
     6541 agggatgcca agtcctggct agtaaaatgc caatgctgaa tgttgaggtc atcaacgaac
     6601 gcgacggtag taatgaaatg gaggaaaacc acggcgatct gcccaaagtg gagaaactgt
     6661 acgtctatcg gaccacagct ggcgcacgag atgacgctcc caatttcgtg aaaattctgg
     6721 gcaagcccat ccccaacccc ctgctgggcc tggacagcac cggcagtgga gagggcagag
     6781 gaagtctgct aacatgcggt gacgtcgagg agaatcctgg cccaatgaaa aagcctgaac
     6841 tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc tccgacctga
     6901 tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga gggcgtggat
     6961 atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa agatcgttat gtttatcggc
     7021 actttgcatc ggccgcgctc ccgattccgg aagtgcttga cattggggaa ttcagcgaga
     7081 gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa
     7141 ccgaactgcc cgctgttctg cagccggtcg cggaggccat ggatgcgatc gctgcggccg
     7201 atcttagcca gacgagcggg ttcggcccat tcggaccgca aggaatcggt caatacacta
     7261 catggcgtga tttcatatgc gcgattgctg atccccatgt gtatcactgg caaactgtga
     7321 tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg ctttgggccg
     7381 aggactgccc cgaagtccgg cacctcgtgc acgcggattt cggctccaac aatgtcctga
     7441 cggacaatgg ccgcataaca gcggtcattg actggagcga ggcgatgttc ggggattccc
     7501 aatacgaggt cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga
     7561 cgcgctactt cgagcggagg catccggagc ttgcaggatc gccgcggctc cgggcgtata
     7621 tgctccgcat tggtcttgac caactctatc agagcttggt tgacggcaat ttcgatgatg
     7681 cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc
     7741 gtacacaaat cgcccgcaga agcgcggccg tctggaccga tggctgtgta gaagtactcg
     7801 ccgatagtgg aaaccgacgc cccagcactc gtccgagggc aaaggaagaa ttctaactag
     7861 agctcgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     7921 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     7981 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     8041 ggacagcaag ggggaggatt gggaagagaa tagcaggcat gctggggagc ggccgccctg
     8101 caggatttaa atgttaggtg ttatggcagt aactttaatt ctagcgatct ggaggcagag
     8161 gcaggtggat ctatgagttt gaggctggct tgctctacac agtgagttcc agaaaagcca
     8221 gggctacaca gtgagaccct atctagaaac aacaacagca acaaaaaata tacacatctc
     8281 tcctaaaaat aaaacacgca gtctggaagt aagtgaccgt tggggaagca ctggaaagga
     8341 gttagagaat gtagacctct tgtgcctgac tgggtgtccg gattcaatag tgacttctcc
     8401 ggtgatctgg gaatctgtga agaggttctg gctctctgtc tagcctgagg ctgatacccc
     8461 aactcatttt cttgtcttct gtgaggaagg ttacaactct aggagtcccc ttcctttggc
     8521 cagtgcttcc ctaagaaagc tatggcttga ttcccacccc caccctacct ccactgccaa
     8581 gtctctggac agatgtgcag cctctggcag tgggagggaa ggaactggat aaagtggtcc
     8641 tcattcgtca gcctcactgg caggcccgga aagtgctgaa ctcagggggc tccggagagg
     8701 atgatgcaga ggtgggcctc ttccgaggcc ctgagtctag ggttgccact gagtcttcct
     8761 gagtcatttt ctttgcccat ggaagaaaag aaaagaaatt taaaaaacaa aaacaaaaca
     8821 aaaaacccta agtggttttt ggtgtaagga tctgggagtt cctcctgttc gaagctgttg
     8881 ggtaccttcc tacctcttcc taccacctcg ccagatttca gattccgacc ttcagtggct
     8941 caaatcccac ttccctaact tcctagcagt ctgacctcac tttgagtgat aacacatatg
     9001 agcctcagtt tcaaatgcta gtatccatct cctaggggca gtatttaaat actggccgtc
     9061 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     9121 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     9181 cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt attttctcct tacgcatctg
     9241 tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag
     9301 ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     9361 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     9421 tcaccgtcat caccgaaacg cgcga
//
