LOCUS       sequence-38297-e         696 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Zhao Y, Araki S, Wu J, Teramoto T, Chang YF, Nakano M, Abdelfattah 
            AS, Fujiwara M, Ishihara T, Nagai T, Campbell RE
  TITLE     An Expanded Palette of Genetically Encoded Ca2+ Indicators.
  JOURNAL   Science. 2011 Sep 30;333(6051):1888-91. doi: 10.1126/science.1208592
  PUBMED    21903779
REFERENCE   2  (bases 1 to 696)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2011-09-30- 30"; volume: "333"; 
            issue: "6051"; pages: "1888-91"
COMMENT     Sequence Label: pTorPE-G-GECO1.1
FEATURES             Location/Qualifiers
     source          1..696
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(6..449)
                     /codon_start=1
                     /product="calcium-binding protein"
                     /label=calmodulin
                     /translation="DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEA
                     ELQDMINEVDADGDGTIDFPEFLTMMARKMNDTDSEEEIREAFRVFDKDGNGYIGAAEL
                     RHVMTNLGEKLTDEEVDEMIRVADIDGDGQVNYEEFVQMMTAK"
     primer_bind     565..584
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 agcttctact tcgctgtcat catttgtaca aactcttcgt agtttacctg accatcccca
       61 tcgatgtctg ctaccctgat catttcatcg acctcttcat ctgttaactt ctctccaagg
      121 tttgtcatca cgtggcgaag ctctgctgcg ccgatgtagc cattgccgtc cttatcaaac
      181 acacggaacg cttctctaat ttcctcttca ctgtctgtgt cattcatttt tcttgccatc
      241 atcgtcagga actcagggaa gtcgattgtg ccgtcaccgt cggcatctac ttcattgatc
      301 atgtcctgca gctctgcttc tgtggggttc tgccccagag accgcatcac cgtccccagc
      361 tccttggttg ttatcgtccc atccccgtcc ttgtcaaata gggagaaagc ctctttaaat
      421 tctgcgatct gctcttcagt cagttggtca cgcgtgttgt actccagctt gtgccccagg
      481 atgttgccgt cctccttgaa gtcgatgccc ttcagctcga tgcggttcac cagggtgtcg
      541 ccctcgaact tcacctcggc gcgggtcttg tagttgccgt cgtccttgaa gaagatggtg
      601 cgctcctgga tgtagccttc gggcatggcg gacttgaaga agtcgtgctg cttcatgtgg
      661 tcggggtagc ggctgaagca ctgcacgccg taggtc
//
