LOCUS       sequence-382973-        4533 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human K202R long-form UBASH3A in Gateway pDONR221 (for N-terminal 
            tag).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4533)
  TITLE     Concannon Lab UBASH3A Plasmids 2022
REFERENCE   2  (bases 1 to 4533)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pDONR221-K202R-long-UBASH3A-Ntag
FEATURES             Location/Qualifiers
     source          1..4533
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(2068..2167)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2184..2203)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2185..2203)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2208..2224)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2208..2224)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2326..2345)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2337..3146
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2404..2423)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3293..3881
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3782..3801
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4035..4052
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4211..4238)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4330..4416)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4465..4487
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4479..4496
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4480..4496
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    join(4512..4533,1..78)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 tgatagtgac ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt
       61 tgtacaaaaa agcaggcttc atggcagcgg gggagacgca gctctacgcc aaggtctcca
      121 acaagctcaa gagccgcagc agcccctcgc tcctggagcc cctcctggcc atgggcttcc
      181 cggtgcacac cgcgctgaaa gcgttggcag ccacggggag gaagacggcg gaggaggcct
      241 tggcctggct gcatgatcat tgcaatgacc cttccctaga cgaccccatc ccccaggagt
      301 atgccctttt cctctgtcca acggggcccc tgctggaaaa acttcaagag ttctggagag
      361 agagcaagcg ccagtgtgca aagaacagag ctcatgaggt cttcccacac gtgacactct
      421 gtgacttctt cacgtgtgaa gaccagaagg tggaatgcct gtacgaggcg ctgaagagag
      481 ctggagacag gctcctgggc tccttcccca cggccgtgcc tctggctctc cactcctcca
      541 tcagctacct cggcttcttc gtcagtggca gccccgcaga cgtcatccgg gaattcgcca
      601 tgaccttcgc cacggaagca tctctcttag caggcacttc cgtttcccgc ttctggattt
      661 tcagccaggt gcctggacat ggccctaacc tgaggctgag caatttaact agagcctcct
      721 tcgtgagcca ctacatcctt caaaaatact gctccgtgaa gccttgcacc aaacagctgc
      781 atctgacctt ggcccacagg ttctaccccc accaccagag gacgctggag cagctggcca
      841 gagccatccc cctgggccac agctgccagt ggaccgcagc actctactcc cgagacatgc
      901 gctttgtgca ctaccagacc ctgagagccc tattccagta caaaccccag aacgtggatg
      961 agctgacgct aagtcctggt gactacatct ttgtggaccc cacgcagcag gacgaagcca
     1021 gcgagggctg ggtgattggg atctcacagc ggacgggctg ccggggcttc ctgccggaaa
     1081 actacacgga tcgagccagt gagtctgaca cgtgggtgaa gcacaggatg tacaccttca
     1141 gtctagccac agacctgaac tccagaaagg atggtgaagc cagcagcaga tgcagcgggg
     1201 aatttcttcc acaaacggca aggagtctta gcagcttaca ggccttgcag gctaccgttg
     1261 caaggaagag tgtgctggtg gttcgccacg gggagagagt ggatcagatc ttcgggaagg
     1321 catggctgca gcaatgctcc actcctgatg ggaaatacta caggccagac ctgaatttcc
     1381 cctgcagtct gcccaggcgg agtcgtggga tcaaagactt tgaaaacgat cccccattat
     1441 catcgtgtgg cattttccag tccagaattg caggggacgc gctactggac agtggtatca
     1501 gaatcagctc tgtgtttgcc tccccagccc tccgctgtgt gcagacggcc aaactcatcc
     1561 tggaagaact caaactggag aaaaaaatca agatacgagt ggaacctgga atctttgaat
     1621 ggacaaaatg ggaagctggc aaaaccaccc caaccctcat gagcctggaa gagctgaaag
     1681 aggcaaattt caacattgac actgattaca ggcccgcgtt tcccctgtcc gccctcatgc
     1741 cggccgagag ctaccaggag tacatggaca ggtgcacggc gagcatggtg caaatcgtca
     1801 acacctgccc gcaggacacg ggtgtcatcc taattgtgag tcacggctcc actctggact
     1861 cctgcacgcg gccactgctc gggctgccgc cccgggaatg tggggatttt gcccaactcg
     1921 tgagaaagat cccttccctg ggcatgtgct tctgtgaaga aaataaagag gaaggaaaat
     1981 gggagttggt gaacccaccg gtgaagaccc tgacccacgg ggcgaacgca gcatttaact
     2041 ggaggaactg gatctcaggc aactgaaacc cagctttctt gtacaaagtt ggcattataa
     2101 gaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca
     2161 ttatttgcca tccagctgat atcccctata gtgagtcgta ttacatggtc atagctgttt
     2221 cctggcagct ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata
     2281 tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg ggtgttatga
     2341 gccatattca acgggaaacg tcgaggccgc gattaaattc caacatggat gctgatttat
     2401 atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt
     2461 atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg
     2521 atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct cttccgacca
     2581 tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg atccccggaa
     2641 aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc
     2701 tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacagcg
     2761 atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttgatgcga
     2821 gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata
     2881 aacttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc
     2941 ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag
     3001 accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac
     3061 agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc
     3121 atttgatgct cgatgagttt ttctaatcag aattggttaa ttggttgtaa cactggcaga
     3181 gcattacgct gacttgacgg gacggcgcaa gctcatgacc aaaatccctt aacgtgagtt
     3241 acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     3301 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     3361 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     3421 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     3481 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     3541 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     3601 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     3661 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     3721 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     3781 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     3841 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     3901 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     3961 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     4021 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     4081 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     4141 ggaaagcggg cagtgagcgc aacgcaatta atacgcgtac cgctagccag gaagagtttg
     4201 tagaaacgca aaaaggccat ccgtcaggat ggccttctgc ttagtttgat gcctggcagt
     4261 ttatggcggg cgtcctgccc gccaccctcc gggccgttgc ttcacaacgt tcaaatccgc
     4321 tcccggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga taaaacgaaa
     4381 ggcccagtct tccgactgag cctttcgttt tatttgatgc ctggcagttc cctactctcg
     4441 cgttaacgct agcatggatg ttttcccagt cacgacgttg taaaacgacg gccagtctta
     4501 agctcgggcc ccaaataatg attttatttt gac
//
