LOCUS       sequence-383059-        2796 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for genome integration of mTurquoise2 CDS to tag native 
            RPL13A for biosensors RibPro and UPRpro.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Torello Pianale L, Olsson L
  TITLE     ScEnSor Kit for Saccharomyces cerevisiae Engineering and 
            Biosensor-Driven Investigation of the Intracellular Environment.
  JOURNAL   ACS Synth Biol. 2023 Aug 8. doi: 10.1021/acssynbio.3c00124.
  PUBMED    37552581
REFERENCE   2  (bases 1 to 2796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2023 Aug 8. doi: 10.1021/acssynbio.3c00124."
COMMENT     Sequence Label: LT1_75_Donor_Tag_RPL13A-mTurq2
FEATURES             Location/Qualifiers
     source          1..2796
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(109..900,901..969))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     732..751
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(970..1074)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1177..1765
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1666..1685
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atccgagcgg ccgcgattat caaaaaggat cttcacctag atccttttaa attaaaaatg
       61 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
      121 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
      181 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
      241 gataccgcgg gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
      301 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
      361 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
      421 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
      481 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
      541 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
      601 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
      661 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
      721 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
      781 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
      841 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
      901 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
      961 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     1021 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     1081 tccccgaaaa gtgccacctg tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     1141 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     1201 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     1261 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     1321 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     1381 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     1441 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     1501 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     1561 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     1621 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     1681 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     1741 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     1801 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     1861 ccgtgcggcc gccaattttc agaaccttag attagccaga tctgagaaga aattcagagg
     1921 tatcagagaa aagagagcta gagaaaaggc tgaagctgaa gctgaaaaga agaaaggatc
     1981 cgtttctaaa ggtgaagaat tattcactgg tgttgtccca attttggttg aattagatgg
     2041 tgatgttaat ggtcacaaat tttctgtctc cggtgaaggt gaaggtgatg ctacttacgg
     2101 taaattgacc ttaaaattta tttgtactac tggtaaattg ccagttccat ggccaacctt
     2161 agtcactact ttatcttggg gtgttcaatg ttttgcaaga tacccagatc atatgaaaca
     2221 acatgacttt ttcaagtctg ccatgccaga aggttatgtt caagaaagaa ctattttttt
     2281 caaagatgac ggtaactaca agaccagagc tgaagtcaag tttgaaggtg ataccttagt
     2341 taatagaatc gaattaaaag gtattgattt taaagaagat ggtaacattt taggtcacaa
     2401 attggaatac aattatttct ctgacaatgt ttacatcact gctgacaaac aaaagaatgg
     2461 tatcaaagct aacttcaaaa ttagacacaa cattgaagat ggtggtgttc aattagctga
     2521 ccattatcaa caaaatactc caattggtga tggtccagtc ttgttaccag acaaccatta
     2581 cttatccact caatctaagt tatccaaaga tccaaacgaa aagagggacc acatggtctt
     2641 gttagaattt gttactgctg ctggtattac cttgggtatg gatgaattgt acaaataaat
     2701 ctgttcaatt tcataaataa tatatatgta caatgtataa acgggaagcc atcacttata
     2761 atttatcatt tccttaactt tatagtctgg gtgaga
//
