LOCUS       sequence-383077-       12690 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  a plasmid modified from miniMOS vector pCFJ910. the modification 
            including insertions of SL2::H2B::GFP downstream to NeoR CDS and 2 
            loxP sites flanking the NeoR and GFP cassettes..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12690)
  AUTHORS   Li J, Qin Y, Shen C, Zhang J, Tu S, Yang J, Wang Y, Zhou R, Zhang K,
            Chen J, Yang W
  TITLE     A new miniMOS tool kit capable of visualizing single copy insertion 
            in C. elegans.
  JOURNAL   PeerJ. 2023 May 17;11:e15433. doi: 10.7717/peerj.15433. eCollection 
            2023.
  PUBMED    37214099
REFERENCE   2  (bases 1 to 12690)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PeerJ."; 
            date: "2023-05-17"; volume: "11:e15433. doi"; pages: " 
            10.7717/peerj.15433. eCollection 2023"
COMMENT     Sequence Label: YW249_Pzipt17-loxP-H2BGFP-miniMOS
FEATURES             Location/Qualifiers
     source          1..12690
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     293..309
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     293..309
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(6166..6199)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             7037..7831
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7091..7110)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7701..7720
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8458..8486)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     9234..9254
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     protein_bind    complement(10002..10035)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     10704..10726
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10764..10782)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10850..10954
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10955..11023,11024..11815)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11173..11192)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11986..12574
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12475..12494
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agctgatacc gcaccgtacg tctcgaggaa ttctatcagg tgtacaagta tgaaatgtcg
       61 tttcgaacat atagatgtct cgcaaacgta aatatttatc gattgtcata aaactttgac
      121 cttgtgaagt gtcaaccttg actgtcgaac caccatagtt tggcgcgaat tgagcgtcat
      181 aattgtttac tctcagtgca gtcaacatgt cgagtttcgt gccgaataaa gagcaaacgc
      241 ggacagtatt aattttctgt tttcatttga agaaaacagc tgcggccgga tccaggaaac
      301 agctatgacc atgattctag ctcgaatttc caactaggag aacattttcg cgcctttctt
      361 ggaaagggag attttatgaa ctccaagtca tattataaat tgattcggaa attttttaga
      421 aagaaaagtg aatggatggc agtgattaaa atttttgtat ttaacaagat gtgaaagctg
      481 caacaatcag tttcacatat ttatgtaaca tgcagtattt ttttagtttt ataaatgaaa
      541 gtacactatt tcttaccgaa aagttctcgt cctcttctct tgttaaacat acttgcacgt
      601 ttcagaagtc tcctggatgc atcgattgag tccactaaaa gttaattatt gttgaaagta
      661 aaaataccaa aaagtgaatt gttcaagaat gtcaaaaaaa aagattcgca atgattcaag
      721 caaaataaat aatttatttt tgatggtgtt tttcgatgtt tttggaacat ttgagctgaa
      781 tgaggaagta tgcatgtttt tgagtgtcta aacaaattca acaacatttg gtaaaatact
      841 tcaagtgaat atgaaaatat gtatatttgt ctttctcttt taggacaaat tttaaaattg
      901 atttttcaaa ctgcataata aattcaggtt gttataccta aacaagctca agatctttaa
      961 cctcaagtct acccgagcta caaaatattt tttagtttac caaatcatca taaattcgcc
     1021 agtttttagt tttccaaatc atcataaatt cgccaaaaat ttttattttg agaaatctca
     1081 cattgattag gcattacacg gaacgctctt aaattcatcg gctcctcttc gtcgaaccac
     1141 atgggtgagg cggcaacaat tgagcatagg gctgaaaatt gtatgttggg gaaataatga
     1201 agatatggga atgattcata tatttcaagt ttatcaattt ttcattttga ggttctctcg
     1261 cagtcccctc gtgttttttt cttttaaaca tatatcagcc aagataattt acacgtggca
     1321 aacattgata catataccta tttttcaatc tgatcatatt aagaagttac gggaatacat
     1381 gttctttatt tttagaatcc aatgaatcca atgaaaacaa atctaaccca attatgcttc
     1441 ttaacattta acgtcatcaa aatattcagt atcgaagtat aaatagagtg cgaagcaaaa
     1501 caaaagttta aaaccggata tgcggtcatc tggtatttac tagtaatctt tcaaaaagca
     1561 aaagatcaag cgatggtaat tcaatccagt tccttatcta agtctgactt aaatgcaagg
     1621 tttcaactta gaatcaatgt gaagtctggg aaaatgtcaa tgaactcaaa cgaagcaaaa
     1681 tttaaacaac aaattggact gtaatccatt ttttcaaaaa tttaatttat ggtattgagt
     1741 tcgaggaaat ttaaacaatt gaatatatga atagatctct ataataccaa aagaacattt
     1801 tagttactca ccaaaaagta gcagaacaac tgagtagttc atcattttcg ctgtagtggg
     1861 cagaaaataa tttggtaagc acacaaccac caatagaagg cgtgcgcttg aaagtgagaa
     1921 tgaatctcat cgttgcggac cctaaaagat atatcacagg gcgcgcgaga agccacgcct
     1981 acctatcaca tttccaaaca gaccgcccgt ttttgagagc tctcatcaaa atggtgacga
     2041 aataattagg ggaagtggag gcgtttgagg attgttgggc gatgtgaaaa gtatcggtta
     2101 ctttgagaaa tgatgaggtt ggtttttgga aagatggatg agactgctgc tgagataacg
     2161 tcatatggaa cgtcatatat ggtttgtaac ggaagtttta aaattttaga gaactgaaaa
     2221 aaaatgaagc gttctcatat ttgctgtaaa caattgcaac aactgtcaaa attccaaaaa
     2281 tagacaatag aaatggtcag ctgtgaaata tcgaatatct gaaaatgaat attcattcac
     2341 ggaagccatg aaaactactg cattgaaaaa tgttacagag tagtcataag ttatttttaa
     2401 gaagaggtcg tctttttttt gtactctagt tgacaaattc taccaataaa taaatataga
     2461 taaaaggggg gaaattgttt ttaaatgtag tatttgagct caaacattca ttcattgcaa
     2521 gggaaaaaac gggaaaatcc gttaacactc agcatttttg aaaaattgac aagtttctga
     2581 aagtggaaat gaattatatt ccacgacatg gccattttag gtcagcagaa tattcattca
     2641 aatttcctat gttaaaacat tattgatttt ttttatcaga cagtttaaga aaactgagag
     2701 aaaggaattg tctaaagtat ttattaaaaa tacaatacaa aatataataa aaacttgaac
     2761 gatcgtcatg acctgggatt tttaaagtca aatttcaaag gtttttttgt cgacaaagct
     2821 ctctcccgtc acgcatcgcg cgagtggaat gcctgatttt aatagtataa atgtaaatag
     2881 caatttgcgt ttacaacatc tagcggcaat gaatgatctt gaacattgac aattgacagg
     2941 agcctcgaaa aaaattccga gataatccat aagcaagtca cgttgtattc aactgatttt
     3001 aatagataaa tagtgtaaaa attctagcaa tcggcaaata gaacttgttg aattaaacac
     3061 acgtgactaa ttcctatttt ttgttttcta ttatatcatt tctatatttc atttctcggt
     3121 tcgacgcttc ttttcttgat ttttgtacag ttcctagcta acagaaggtg agtaacactt
     3181 tgattgaaaa agaatgtctt taaacgagac catttaatat tataccgctt ttttgattta
     3241 gaacttgctg aattaattaa caaaaacaaa atacagaacg gataatctgt ttacaacatt
     3301 tctaattagg tgttttcggg acgccttttt taaagcataa gaacacttgc ccttcctctg
     3361 ctaacagagc gtttctatgt gtttactcaa cgttttttca ccaacaaaaa catttcccgt
     3421 aaacgcatta ataccacttt tacaaaaata aacatagttt ttctagcagt gcacctttct
     3481 tgcaaaagtg caacaatttt acgacatact actttttttt aacggatagt tggacgaacc
     3541 aaagttcaac tttctgtaat gacctagtct acaaagcaaa ctttacataa ttagttgaat
     3601 ggtaagtgta ccgttttttt aaaaataatt tcgtttcgaa tctagaacta tccggaacag
     3661 agccataccg tcgttttcgc ataattcttg ccaagttcct cttgaaaacc ggataaagtg
     3721 cgggcgcccg ttgcccattt cctattcctc ccaccccaat ccactgatat ttgatatctt
     3781 atttgtacct ctaaactctc ttccatttac ccacactctc aatctttcca tatttttctt
     3841 tcactatctg ctgcttttag tggactgtcc acttttattt gacttccgtt tcttatattg
     3901 gcaagttgaa cacattttta cactgaaatt tcaatttctt taacgtaaaa agtacagatc
     3961 gagaacttta ttctggtttt taaaatacct gttttccatc gctctttcct aagtttttat
     4021 taaattacag tttcaaaata atactctaca taaaatgatg tccgaaggtc ctgttatgtt
     4081 cgatacagta ctctatggtg ttatctcagt cacggttgtc tcattattat ccttgggtgg
     4141 tgcatgcatt ggcccactaa tgacaaaaga tgcaaaacat cggtgagctt gggttggagt
     4201 tgaaactgta tttattgcca aattttaaga tggctacatt tttttttggc aatggccgtg
     4261 tctacactga gttcggatgc aattctacat ataattccgc aggtgaaatt actaatgatt
     4321 caaaaataat gccctgactt tttcgcacgc taaagttttc ttgcatttaa aggattaaag
     4381 tttccaaaat ataaattaag tgaaaaagtt ttgattttag gttgtcggcg tccatgatca
     4441 tgggcacaat cacggacata gccatgaaca tcatcatgag catacagatt tggaaaatgg
     4501 aacatcagga cacgggcata gtcatcacca tgatgatagt gaccgcatta tggtgagata
     4561 tcatttaaaa tagtatcttt gcatttctag aattttcgaa aaaaatttga aatttttctg
     4621 aacgaaaaca ttttaactga aattaatttg gaataaacat atttcagtgg tataccgtca
     4681 cacctgctcg taaaaatctt cttcgtcttt ctgttattgt tctctccatc tacattctct
     4741 actttgtaga gtttttcatg ttctacagaa agacccatct cgtgagtttc atcccaaaac
     4801 cttttgccac aactttacct ttgtagcact actgttcccc ttctacaaag tcgccagtta
     4861 ttacatcttc agtttccaca cactctgaca tcaaatcttc agcttcaaat catagtataa
     4921 ctggtagcga agaagataat aataatgatt catcgaaaaa agaaagaaga aatagtgttg
     4981 agttggagaa gcgcagagag caaggaggac acgagctgat cagtttaaga gaggatggtg
     5041 atgatgatgg tacagagatt tgcggactga aagtgagttt tatttgaaat agttggacgg
     5101 tactacaacg acaattccag ccccgagcat tgataatttt attcggtgat ggtgtacaca
     5161 atttggtcga tggtcttgca atgggagctt cattcatgat ttccgtgaaa ctcggattca
     5221 taacaaccat cgcagtaatt tgtcacgaac ttcctcatga aattggagat ctggcggtgc
     5281 tcatagattc tgggctttca atgtgtacag ctcttattct aaatttatta tctgccctga
     5341 ctgcatacgc cggcttgttc attgcaatcg ttctgggaag agatgaagaa attgagacca
     5401 tccttctggc gatcacagct ggaatgtttt tgtatgttgc ctgggtggat atggcaagtt
     5461 taaacacatt gattgtattt aataatttat attatagctt tcacatctga aacatgattc
     5521 tcttatggcg gatcactgga tggtgacaag tatattacag ctctctggtt tcacattggg
     5581 ctttgcgctc attttcggat taggatggtt tgagcatatg ctggaaggat aacattaaca
     5641 tattcaaaga caaaaaacga tgataacggg tttacttgaa atgtgataat aaagtgatta
     5701 ttccaaatgt attatactaa aagaaactta aaaatttccg tcttaatttt ttaaagttat
     5761 ttcgtctttg agaaatttga gattgtggaa tttttgttat tatttcttaa ttggactcag
     5821 tattttcaat ttttaatata ttgaattgtt caagtttgta gtaatatggt acctgcttga
     5881 ttagtggaat gaatggaaaa cattagaaaa tgatgctttt tggcagattc aaaactcctt
     5941 gaccgtcgtg gtgcagtcat atggtctcca cgtcccactt tttgcaaatg agccgccatt
     6001 atttttggat aaaatcatct gcagtgaaaa aataatttta gttagtttgt gaatttttca
     6061 ataccgtata acaagagcct gcggttccta cataacctga tgcgacgtta ttgtagcttc
     6121 ctatgtgctg tatccctcga ggtcgacagg cctgcatgcg tcgacataac ttcgtatagc
     6181 atacattata cgaagttata gttgcaggac cactgcggcc gcatgggcca tggcacctta
     6241 acatttctaa attttagttt aataaaaaaa ttttaaaata atacgagttt tgagactaac
     6301 tttcaataaa aaccaaaaaa ttgcttcaaa aaagagctcg cgccgattta cgataaacac
     6361 agcgcgctac gacactccga aaaccgcgcc gcaccattac attctccgcc ccaccgcatg
     6421 gtttgcggaa ggcgatgaaa ttgctgggcc taaaatttta accctttttt tcccgttttt
     6481 caactgtttt cccgattttt tcgatatttt tttcaggttt ttagttagaa aagtatataa
     6541 aaattgcatt atttgtagtt ttcgctcgta atttgtgata gaaaacggcc aatttgccta
     6601 gatttgtgat tctaccaagt ggaatttttg ggaaaatatc aatattccac gatttttatt
     6661 caaatcataa cgtttcgaaa ttatcagctt aaataaaact aaaataaata ataaaatact
     6721 aaaaaccctg aatttctcgg ttctcggttc agcttgagtt cgttctatac acgttatcga
     6781 tttttgtcat ttcgtttaat catgggtttt gattcatttt aaatatgaaa tttgatttat
     6841 tcctcgtttc gaagttggtt tgtcttgtcc ggccggtaaa caaatgttat taaaaaaaat
     6901 aataaatttc gaaatttttc gtaatgttcc gcaatcgtgt ttttccgttt atctcgtctt
     6961 cccttccttc aaatatacta tttattttct caattcacat attttcagcc agccctgctc
     7021 aacaagtgga ataaaaatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     7081 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     7141 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     7201 tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     7261 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     7321 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     7381 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     7441 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     7501 ggatgatctg gacgaagagc atcaggggct cgcaccagcc gaactgttcg ccaggctcaa
     7561 ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     7621 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     7681 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     7741 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     7801 cttctatcgc cttcttgacg agttcttcta gccatgttgt taccttgtat taaaacaatg
     7861 tttcaagttg aatttgttct tgttgttgaa ttctattcac atggtcttta cccatgcttc
     7921 atatcttatt gttttcttac cctggtcact ttgtggcatt aatactttct ttcagcgttc
     7981 tatcaaacgt ttgttctctt tggagctaag gcaaaccgcg caacttacga ttgaagaatg
     8041 ccaccaaagc catctgccaa gggagccaag aaggccgcca agaccgttac gaagccaaag
     8101 gacggaaaga agagacgtca tgcccgtaag gaatcatact ccgtctacat ctaccgtgtc
     8161 ctcaagcaag ttcatccaga cactggagtt tcctccaaag ccatgtctat catgaactct
     8221 tttgtcaacg atgtcttcga gcgtattgct gctgaagcat cccgtcttgc tcactacaac
     8281 aagcgttcca caatctcatc ccgcgaaatt cagaccgctg tccgtctgat ccttccagga
     8341 gagcttgcca agcacgccgt gtctgaggga accaaggccg ttaccaagta cacttccagc
     8401 aagggctcgg gaggcggagg tggctctatg agtaaaggag aagaactttt cactggagtt
     8461 gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga
     8521 gagggtgaag gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga
     8581 aaactacctg ttccatgggt aagtttaaac atatatatac taactaaccc tgattattta
     8641 aattttcagc caacacttgt cactactttc tgttatggtg ttcaatgctt ctcgagatac
     8701 ccagatcata tgaaacggca tgactttttc aagagtgcca tgcccgaagg ttatgtacag
     8761 gaaagaacta tatttttcaa agatgacggg aactacaaga cacgtaagtt taaacagttc
     8821 ggtactaact aaccatacat atttaaattt tcaggtgctg aagtcaagtt tgaaggtgat
     8881 acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg aaacattctt
     8941 ggacacaaat tggaatacaa ctataactca cacaatgtat acatcatggc agacaaacaa
     9001 aagaatggaa tcaaagttgt aagtttaaac atgattttac taactaacta atctgattta
     9061 aattttcaga acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac
     9121 cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac
     9181 ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt
     9241 cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata caaataagtc
     9301 caattactct tcaacatccc tacatgctct ttctccctgt gctcccaccc cctatttttg
     9361 ttattatcaa aaaacttctc ttaatttctt tgttttttag cttcttttaa gccacctcta
     9421 acaatgaaat tgtgtagatt caaaaataga attaattcgt aataaaaagt cgaaaaaaat
     9481 tgtgctccct ccccccatta ataataattc tatcccaaaa tctacacaat gttctgtgta
     9541 cacttcttat gttttttact tctgataaat ttttttgaaa catcatagaa aaaaccgcac
     9601 acaaaatacc ttatcatatg ttacgtttca gtttatgacc gcaattttta tttcttcgca
     9661 cgtctgggcc tctcatgacg tcaaatcatg ctcatcgtga aaaagttttg gagtattttt
     9721 ggaatttttc aatcaagtga aagtttatga aattaatttt cctgcttttg ctttttgggg
     9781 tttcccctat tgtttgtcaa gatttcgagg acggcgtttt tcttgctaaa atcacaagta
     9841 ttgatgagca cgatgcaaga aagatcggaa gaaggtttgg gtttgaggct cagtggaagg
     9901 tgagtagaag ttgataattt gaaagtggag tagtgtctat ggggtttttg ccttaaatga
     9961 cagaatacat tcccaatata ccaaacataa ctgtttctcg aataacttcg tatagcatac
    10021 attatacgaa gttatggtcg acggtatcga taagcttgat ccggatctgc ggcttactca
    10081 ccagacctgg ccccatccga ttaccaccta ttcgcttcga tgggacacgc actcgctgag
    10141 cagcgcttcg attcttacga aagtgtgaaa aaatggctcg atgaatggtt cgccgcaaaa
    10201 gacgatgagt tctactggcg tggaatccac aaattgcccg agagatggga aaaatgtgta
    10261 gctagcgacg gcaaatactt tgaataaatg attttttctt tttccacaaa atttaacgtg
    10321 ttttttgatt taaaaaaaac gacatttcat acttgtacac ctgatatcta gatacctagg
    10381 tgagctctgg tacccccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
    10441 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
    10501 cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
    10561 acaccgcata tggtgcactc tcagtgcaat ctgctctgat gccgcatagt taagccagcc
    10621 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
    10681 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
    10741 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
    10801 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
    10861 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
    10921 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
    10981 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
    11041 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
    11101 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
    11161 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
    11221 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
    11281 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
    11341 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
    11401 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
    11461 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
    11521 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
    11581 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
    11641 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggtc
    11701 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
    11761 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
    11821 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
    11881 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
    11941 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
    12001 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
    12061 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
    12121 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
    12181 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
    12241 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
    12301 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
    12361 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
    12421 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
    12481 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
    12541 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
    12601 gttcctggtc ttttgctggc cttttgttca catgttcttt cctgcgttat cccctgattc
    12661 tgtggataac cgtattaccg cctttgagtg
//
