LOCUS       sequence-383078-        8935 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mCherry from the POU3F2 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8935)
  AUTHORS   Zhang Y, Belote RL, Urquijo MA, Hansen MMK, Hejna M, Moustafa TE, 
            Liu T , Lange D , Vand-Rajabpour F, Chang M, Lohman BK, Stubben C, 
            Zhang X, Weinberger LS, VanBrocklin MW, Grossman D , Lex A, Kulkarni
            R , Zangle T, Judson-Torres RL
  TITLE     A BRN2:MYC transcriptional axis regulates interconversion between 
            therapy-resistant and tumorigenic phenotypes in melanoma
  JOURNAL   Cell Reports (2025)
REFERENCE   2  (bases 1 to 8935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Reports (2025)"
COMMENT     Sequence Label: pROM-POU3F2p-mCherry-Neo
FEATURES             Location/Qualifiers
     source          1..8935
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(9..28)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     402..425
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     583..599
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     584..600
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    603..1191
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             709..720
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     1118..1138
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1248..1955)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(1526..1545)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1795..1813
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             complement(1974..1994)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    3081..3198
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(3496..3540)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3725..3958
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    4455..4580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             4627..4807
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(4825..5023)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4850..4870)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5025..5404
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     5576..5595
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        5670..5774
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5775..5843,5844..6635)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5993..6012)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6806..7394
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7295..7314
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     LTR             7456..7636
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     CDS             complement(8081..8875)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(8081..8875)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8192..8211)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     8802..8821
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(join(8896..8935,1..460))
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
ORIGIN
        1 gacgtgcgct tttgaagcgt gcagaatgcc gggcctccgg aggaccttcg ggcgcccgcc
       61 ccgcccctga acccgcccct gagcccgccc ccggacccac cccttcccag cctctgagcc
      121 cagaaagcga aggagcaaag ctgctattgg ccgctgcccc aaaggcctac ccgcttccat
      181 tgctcagcgg tgctgtccat ctgcacgaga ctagtgagac gtgctacttc catttgtcac
      241 gtcctgcacg acgcgagctg cggggcgggg gggaacttcc tgactagggg aggagtagaa
      301 ggtggcgcga aggggccacc aaagaacgga gccggttggc gcctaccggt ggatgtggaa
      361 tgtgtgcgag gccagaggcc acttgtgtag cgccaagtgc ccagcggggc tgctaaagcg
      421 catgctccag actgccttgg gaaaagcgcc tcccctaccc gtacctgagg tgtgactgga
      481 aaacccacct cctcctcctc ttgtgcttct agccaggcac aatcagcatt ggtagctgct
      541 gtattgctac ttgtgattgc tccatgtttt tctaggtctc gatcgaggtc gacggtatcg
      601 atgcggggag gcggcccaaa gggagatccg actcgtctga gggcgaaggc gaagacgcgg
      661 aagaggccgc agagccggca gcaggccgcg ggaaggaagg tccgctggat tgagggccga
      721 agggacgtag cagaaggacg tcccgcgcag aatccaggtg gcaacacagg cgagcagcca
      781 aggaaaggac gatgatttcc ccgacaacac cacggaattg tcagtgccca acagccgagc
      841 ccctgtccag cagcgggcaa ggcaggcggc gatgagttcc gccgtggcaa tagggagggg
      901 gaaagcgaaa gtcccggaaa ggagctgaca ggtggtggca atgccccaac cagtgggggt
      961 tgcgtcagca aacacagtgc acaccacgcc acgttgcctg acaacgggcc acaactcctc
     1021 ataaagagac agcaaccagg atttatacaa ggaggagaaa atgaaagcca tacgggaagc
     1081 aatagcatga tacaaaggca ttaaagcagc gtatccacat agcgtaaaag gagcaacata
     1141 gttaagaata ccagtcaatc tttcacaaat tttgtaatcc agaggttgat tatcgataag
     1201 cttgatatcg aattgtacct agtggaaccg gaacccttaa agctagctta cttgtacagc
     1261 tcgtccatgc cgccggtgga gtggcggccc tcggcgcgtt cgtactgttc cacgatggtg
     1321 tagtcctcgt tgtgggaggt gatgtccaac ttgatgttga cgttgtaggc gccgggcagc
     1381 tgcacgggct tcttggcctt gtaggtggtc ttgacctcag cgtcgtagtg gccgccgtcc
     1441 ttcagcttca gcctctgctt gatctcgccc ttcagggcgc cgtcctcggg gtacatccgc
     1501 tcggaggagg cctcccagcc catggtcttc ttctgcatta cggggccgtc ggaggggaag
     1561 ttggtgccgc gcagcttcac cttgtagatg aactcgccgt cctgcaggga ggagtcctgg
     1621 gtcacggtca ccacgccgcc gtcctcgaag ttcatcacgc gctcccactt gaagccctcg
     1681 gggaaggaca gcttcaagta gtcggggatg tcggcggggt gcttcacgta ggccttggag
     1741 ccgtacatga actgagggga caggatgtcc caggcgaagg gcagggggcc acccttggtc
     1801 accttcagct tggcggtctg ggtgccctcg taggggcggc cctcgccctc gccctcgatc
     1861 tcgaactcgt ggccgttcac ggagccctcc atgtgcacct tgaagcgcat gaactccttg
     1921 atgatggcca tgttatcctc ctcgcccttg ctcacactag ttccgcctcc tcccaccttg
     1981 cgcttcttct tgggaggtcc aggcatggat ccagcgggcc gctctttctg tctcctctct
     2041 ctcccacagc gggcagctcc gacgcccgct ccgacgcctt ctgttgctgc tcctgctatt
     2101 actgctgctg ctgccgccgc cgccgcctcc cgcccgccct cgctctcttt ctcctcctct
     2161 ccctccttct cgctctcgct cactctctga ctagctcggc gctcccccct ctccctgctc
     2221 tctccagctc tctcccgccc tcttggcagc gccggctcgc agcggcacgc gcctggaccc
     2281 cgccccctcc gccccctccc attggctccg cgccctttga tttacgtgga taccgctagc
     2341 aacctcccag gccggcgcca ctgattggcg cagagctact ccctccccct cccccgggct
     2401 cggagtcctc ctagccctcc tccccgtccc cttcgcctcc cgattcagat tctctcagtt
     2461 ctcacccccc cgcttcttcc tcacccgggg cctcctccgc cctctctcgg atgggccccg
     2521 ccccccatct gtctcccagg cagagaagac cggcgggggg gcggtcccgg tgctcttatc
     2581 ggccacagac acttggaaga cttggattgg ggtggggggc gggaggcagt ggtgtccact
     2641 ttcgtttccc aatagggctt taagatgggg aaacggaggg ggtaagggaa aagctgggtc
     2701 gcaccgagcc agcgggagtc ggattttatt cacctaggga cacacgcaca gtgtgtatct
     2761 cttaaaccta gagccaagag ccagacctgt tcccctgttc aactctcctt gcatcccacg
     2821 gttagaattt cagtgcacaa gtgtctcttg ctccagggtg tgcggacact tctgcccaaa
     2881 gctcttctcc ttcccctcta ccccgcggct agcacaggct ctcttccttt taggttccag
     2941 gcccaattgt ctcccactcc cttggggaaa gaaccccgag agaggcaggc tcagcccgca
     3001 ccgcggcttg taagtcattc ccatcaaggc tggcccggta ctaaccaaac tggatctctg
     3061 ctgtccctgt aataaacccg aaaattttga atttttgtaa tttgtttttg taattcttta
     3121 gtttgtatgt ctgttgctat tatgtctact attctttccc ctgcactgta ccccccaatc
     3181 cccccttttc ttttaaaatt gtggatgaat actgccattt gtctgcagaa ttggcgcacg
     3241 cagtgccgat ccgttcacta atcgaatgga tctgtctctg tctctctctc caccttcttc
     3301 ttctattcct tcgggcctgt cgggtcccct cggggttggg aggtgggtct gaaacgataa
     3361 tggtgaatat ccctgcctaa ctctattcac tatagaaagt acagcaaaaa ctattcttaa
     3421 acctaccaag cctcctacta tcattatgaa taattttata taccacagcc aatttgttat
     3481 gttaaaccaa ttccacaaac ttgcccattt atctaattcc aataattctt gttcattctt
     3541 ttcttgctgg ttttgcgatt cttcaattaa ggagtgtatt aagcttgtgt aattgttaat
     3601 ttctctgtcc cactccatcc aggtcgtgtg attccaaatc tgttccagag atttattact
     3661 ccaactagca ttccaaggca cagcagtggt gcaaatgagt tttccagagc aaccccaaat
     3721 ccccaggagc tgttgatcct ttaggtatct ttccacagcc aggattcttg cctggagctg
     3781 cttgatgccc cagactgtga gttgcaacag atgctgttgc gcctcaatag ccctcagcaa
     3841 attgttctgc tgctgcacta taccagacaa taattgtctg gcctgtaccg tcagcgtcat
     3901 tgacgctgcg cccatagtgc ttcctgctgc tcccaagaac ccaaggaaca aagctcctat
     3961 tcccactgct cttttttctc tctgcaccac tcttctcttt gccttggtgg gtgctactcc
     4021 taatggttca atttttacta ctttatattt atataattca cttctccaat tgtccctcat
     4081 atctcctcct ccaggtctga agatcagcgg ccggccgctt gctgtgcggt ggtcttactt
     4141 ttgttttgct cttcctctat cttgtctaaa gcttccttgg tgtcttttat ctctatcctt
     4201 tgatgcacac aatagagggt tgctactgta ttatataatg atctaagttc ttctgatcct
     4261 gtctgaaggg atggttgtag ctgtcccagt atttgtctac agccttctga tgtttctaac
     4321 aggccaggat taactgcgaa tcgttctagc tccctgcttg cccatactat atgttttaat
     4381 ttatattttt tctttccccc tggccttaac cgaatttttt cccatcgcga tctaattctc
     4441 ccccgcttaa tactgacgct ctcgcaccca tctctctcct tctagcctcc gctagtcaaa
     4501 atttttggcg tactcaccag tcgccgcccc tcgcctcttg ccgtgcgcgc ttcagcaagc
     4561 cgagtcctgc gtcgagagag ctcctctggt ttccctttcg ctttcaagtc cctgttcggg
     4621 cgccactgct agagattttc cacactgact aaaagggtct gagggatctc tagttaccag
     4681 agtcacacaa cagacgggca cacactactt gaagcactca aggcaagctt tattgaggct
     4741 taagcagtgg gttccctagt tagccagaga gctcccaggc tcagatctgg tctaaccaga
     4801 gagacccagt acaggcaaaa cgcgctgctt atatagacct cccaccgtac acgcctaccg
     4861 cccatttgcg tcaatggggc ggagttgtta cgacattttg gaaagtcccg ttgattttgg
     4921 tgccaaaaca aactcccatt gacgtcaatg gggtggagac ttggaaatcc ccgtgagtca
     4981 aaccgctatc cacgcccatt gatgtactgc caaaaccgca tcaccatggt aatagcgatg
     5041 actaatacgt agatgtactg ccaagtagga aagtcccata aggtcatgta ctgggcataa
     5101 tgccaggcgg gccatttacc gtcattgacg tcaatagggg gcgtacttgg catatgatac
     5161 acttgatgta ctgccaagtg ggcagtttac cgtaaatact ccacccattg acgtcaatgg
     5221 aaagtcccta ttggcgttac tatgggaaca tacgtcatta ttgacgtcaa tgggcggggg
     5281 tcgttgggcg gtcagccagg cgggccattt accgtaagtt atgtaacgcg gaactccata
     5341 tatgggctat gaactaatga ccccgtaatt gattactatt aataactagt caataatcaa
     5401 tgtcaacgcg tatatctggc ccgtacatcg cgaagcagcg caaaacgcct aaccctaagc
     5461 agattcttca tgcaattgtc ggtcaagcct tgccttgttg tagcttaaat tttgctcgcg
     5521 cactactcag cgacctccaa cacacaagca gggagcagat actggcttaa ctatgcggca
     5581 tcagagcaga ttgtactgag agtgcaccat aggggatcgg gagatctccc gatccgtcga
     5641 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     5701 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     5761 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     5821 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     5881 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     5941 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     6001 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     6061 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     6121 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     6181 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     6241 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     6301 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     6361 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     6421 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     6481 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     6541 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     6601 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     6661 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     6721 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     6781 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     6841 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     6901 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     6961 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     7021 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     7081 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     7141 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     7201 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     7261 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     7321 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     7381 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     7441 cttttgctca catgctgcta gagattttcc acactgacta aaagggtctg agggatctct
     7501 agttaccaga gtcacacaac agacgggcac acactacttg aagcactcaa ggcaagcttt
     7561 attgaggctt aagcagtggg ttccctagtt agccagagag ctcccaggct cagatctggt
     7621 ctaaccagag agacccagta cagtccggat gcagctctcg ggccatgtga tgaaatgcta
     7681 ggcggctgtc aaacctccac tctaatactt ctctctccgg gtcatccatc ccatgcaggc
     7741 tcacagggtg taacaagcgg gtgttctctc cttcattggc ttcttctacc ttctcttgct
     7801 caactggtac tagcttgtag caccatccaa aggtcagtgg atatctgatc cctggccctg
     7861 gtgtgtagtt ctgccaatca gggaagtagc cttgtgtgtg gtagatccac agatcaagga
     7921 tatcttgtct tcgttgggag tgaattagcc cttccagtcc ccccttttct tttaaaaagt
     7981 ggctaagatc tacagctgcc ttgtaagtca ttggtcttaa aggtactgca tggggtcgtg
     8041 cgctcctttc ggtcgggcgc tgcgggtcgt ggggcgggcg tcagaagaac tcgtcaagaa
     8101 ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc
     8161 ggtcagccca ttcgccgcca agctcttcag caatatcacg ggtagccaac gctatgtcct
     8221 gatagcggtc cgccacaccc agccggccac agtcgatgaa tccagaaaag cggccatttt
     8281 ccaccatgat attcggcaag caggcatcgc catgggtcac gacgagatcc tcgccgtcgg
     8341 gcatgctcgc cttgagcctg gcgaacagtt cggctggcgc gagcccctga tgctcttcgt
     8401 ccagatcatc ctgatcgaca agaccggctt ccatccgagt acgtgctcgc tcgatgcgat
     8461 gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc cgccgcattg
     8521 catcagccat gatggatact ttctcggcag gagcaaggtg agatgacagg agatcctgcc
     8581 ccggcacttc gcccaatagc agccagtccc ttcccgcttc agtgacaacg tcgagcacag
     8641 ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg cgctgcctcg tcttgcagtt
     8701 cattcagggc accggacagg tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca
     8761 gccggaacac ggcggcatca gagcagccga ttgtctgttg tgcccagtca tagccgaata
     8821 gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca atcatggtaa
     8881 gcttgggctg caggtcgaaa ggcccggaga tgaggaagag gagaacagcg cggca
//
