LOCUS       sequence-383081-        9432 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Cas9 and sgRNA cassettes for targeting the HO locus in 
            yeast Saccharomyces cerevisiae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9432)
  AUTHORS   Torello Pianale L, Olsson L
  TITLE     ScEnSor Kit for Saccharomyces cerevisiae Engineering and 
            Biosensor-Driven Investigation of the Intracellular Environment.
  JOURNAL   ACS Synth Biol. 2023 Aug 8. doi: 10.1021/acssynbio.3c00124.
  PUBMED    37552581
REFERENCE   2  (bases 1 to 9432)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2023 Aug 8. doi: 10.1021/acssynbio.3c00124."
COMMENT     Sequence Label: YN2_1_IL50_HOlocus
FEATURES             Location/Qualifiers
     source          1..9432
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_RNA        284..359
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             1183..5286
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             1183..5286
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5299..5319
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5323..5343
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     gene            5674..7029
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     CDS             6018..6827
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(6085..6104)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      6832..7029
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     misc_feature    7034..7537
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     CDS             complement(join(7648..8439,8440..8508))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8271..8290
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8509..8613)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      8716..9304
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9205..9224
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaatgtgctt cagtattaca ttttttgcct tcaacgcctt gattgttcta tttttgctaa
       61 taataaatct atttcatcgg actaaaagtc cattagttgt aagcggattt agctcagttg
      121 ggagagcgcc agactgaaga aaaacttcgg tcaagtcatc tggaggtcct gtgttcgatc
      181 cacagaattc gcagatggcc ggcatggtcc cagcctcctc gctggcgccg gctgggcaac
      241 accttcgggt ggcgaatggg actgctccag cattatagca tgcgttttag agctagaaat
      301 agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct
      361 ttttttattt tttgtcacta ttgttatgta aaatgccacc tctgacagta tggaacgcaa
      421 acttctgtct agtggatact gatgagacca aaccagatgt caacacagct acaacggtga
      481 gtaaggaaag agtgaggaac tatcgcatac ctgcatttaa agatgccgat ttgggcgcga
      541 atcctttatt ttggcttcac cctcatacta ttatcagggc cagaaaaagg aagtgtttcc
      601 ctccttcttg aattgatgtt accctcataa agcacgtggc ctcttatcga gaaagaaatt
      661 accgtcgctc gtgatttgtt tgcaaaaaga acaaaactga aaaaacccag acacgctcga
      721 cttcctgtca tcctattgat tgcagcttcc aatttcgtca cacaacaagg tcctagcgac
      781 ggctcacagg ttttgtaaca agcaatcgaa ggttctggaa tggcgggaaa gggtttagta
      841 ccacatgcta tgatgcccac tgtgatctcc agagcaaagt tcgttcgatc gtactgttac
      901 tctctctctt tcaaacagaa ttgtccgaat cgtgtgacaa caacagcctg ttctcacaca
      961 ctcttttctt ctaaccaagg gggtggttta gtttagtaga acctcgtgaa acttacattt
     1021 acatatatat aaacttgcat aaattggtca atgcaagaaa tacatatttg gtcttttcta
     1081 attcgtagtt tttcaagttc ttagatgctt tctttttctc ttttttacag atcatcaagg
     1141 aagtaattat ctacttttta caacaaatat aaaacaagat ctatggacaa gaagtattct
     1201 atcggactgg atatcgggac taatagcgtc gggtgggccg tgatcactga cgagtacaag
     1261 gtgccctcta agaagttcaa ggtgctcggg aacaccgacc ggcattccat caagaaaaat
     1321 ctgatcggag ctctcctctt tgattcaggg gaaaccgctg aagcaacccg cctcaagcgg
     1381 actgctagac ggcggtacac caggaggaag aaccggattt gttaccttca agagatattc
     1441 tccaacgaaa tggcaaaggt cgacgacagc ttcttccata ggctggaaga atcattcctc
     1501 gtggaagagg ataagaagca tgaacggcat cccatcttcg gtaatatcgt cgacgaggtg
     1561 gcctatcacg agaaataccc aaccatctac catcttcgca aaaagctggt ggactcaacc
     1621 gacaaggcag acctccggct tatctacctg gccctggccc acatgatcaa gttcagaggc
     1681 cacttcctga tcgagggcga cctcaatcct gacaatagcg atgtggataa actgttcatc
     1741 cagctggtgc agacttacaa ccagctcttt gaagagaacc ccatcaatgc aagcggagtc
     1801 gatgccaagg ccattctgtc agcccggctg tcaaagagcc gcagacttga gaatcttatc
     1861 gctcagctgc cgggtgaaaa gaaaaatgga ctgttcggga acctgattgc tctttcactt
     1921 gggctgactc ccaatttcaa gtctaatttc gacctggcag aggatgccaa gctgcaactg
     1981 tccaaggaca cctatgatga cgatctcgac aacctcctgg cccagatcgg tgaccaatac
     2041 gccgaccttt tccttgctgc taagaatctt tctgacgcca tcctgctgtc tgacattctc
     2101 cgcgtgaaca ctgaaatcac caaggcccct ctttcagctt caatgattaa gcggtatgat
     2161 gagcaccacc aggacctgac cctgcttaag gcactcgtcc ggcagcagct tccggagaag
     2221 tacaaggaaa tcttctttga ccagtcaaag aatggatacg ccggctacat cgacggaggt
     2281 gcctcccaag aggaatttta taagtttatc aaacctatcc ttgagaagat ggacggcacc
     2341 gaagagctcc tcgtgaaact gaatcgggag gatctgctgc ggaagcagcg cactttcgac
     2401 aatgggagca ttccccacca gatccatctt ggggagcttc acgccatcct tcggcgccaa
     2461 gaggacttct acccctttct taaggacaac agggagaaga ttgagaaaat tctcactttc
     2521 cgcatcccct actacgtggg acccctcgcc agaggaaata gccggtttgc ttggatgacc
     2581 agaaagtcag aagaaactat cactccctgg aacttcgaag aggtggtgga caagggagcc
     2641 agcgctcagt cattcatcga acggatgact aacttcgata agaacctccc caatgagaag
     2701 gtcctgccga aacattccct gctctacgag tactttaccg tgtacaacga gctgaccaag
     2761 gtgaaatatg tcaccgaagg gatgaggaag cccgcattcc tgtcaggcga acaaaagaag
     2821 gcaattgtgg accttctgtt caagaccaat agaaaggtga ccgtgaagca gctgaaggag
     2881 gactatttca agaaaattga atgcttcgac tctgtggaga ttagcggggt cgaagatcgg
     2941 ttcaacgcaa gcctgggtac ttaccatgat ctgcttaaga tcatcaagga caaggatttt
     3001 ctggacaatg aggagaacga ggacatcctt gaggacattg tcctgactct cactctgttc
     3061 gaggaccggg aaatgatcga ggagaggctt aagacctacg cccatctgtt cgacgataaa
     3121 gtgatgaagc aacttaaacg gagaagatat accggatggg gacgccttag ccgcaaactc
     3181 atcaacggaa tccgggacaa acagagcgga aagaccattc ttgatttcct taagagcgac
     3241 ggattcgcta atcgcaactt catgcaactt atccatgatg attccctgac ctttaaggag
     3301 gacatccaga aggcccaagt gtctggacaa ggtgactcac tgcacgagca tatcgcaaat
     3361 ctggctggtt cacccgctat taagaagggt attctccaga ccgtgaaagt cgtggacgag
     3421 ctggtcaagg tgatgggtcg ccataaacca gagaacattg tcatcgagat ggccagggaa
     3481 aaccagacta cccagaaggg acagaagaac agcagggagc ggatgaaaag aattgaggaa
     3541 gggattaagg agctcgggtc acagatcctt aaagagcacc cggtggaaaa cacccagctt
     3601 cagaatgaga agctctatct gtactacctt caaaatggac gcgatatgta tgtggaccaa
     3661 gagcttgata tcaacaggct ctcagactac gacgtggacc atatcgtccc tcagagcttc
     3721 ctcaaagacg actcaattga caataaggtg ctgactcgct cagacaagaa ccggggaaag
     3781 tcagataacg tgccctcaga ggaagtcgtg aaaaagatga agaactattg gcgccagctt
     3841 ctgaacgcaa agctgatcac tcagcggaag ttcgacaatc tcactaaggc tgagaggggc
     3901 ggactgagcg aactggacaa agcaggattc attaaacggc aacttgtgga gactcggcag
     3961 attactaaac atgtcgccca aatccttgac tcacgcatga ataccaagta cgacgaaaac
     4021 gacaaactta tccgcgaggt gaaggtgatt accctgaagt ccaagctggt cagcgatttc
     4081 agaaaggact ttcaattcta caaagtgcgg gagatcaata actatcatca tgctcatgac
     4141 gcatatctga atgccgtggt gggaaccgcc ctgatcaaga agtacccaaa gctggaaagc
     4201 gagttcgtgt acggagacta caaggtctac gacgtgcgca agatgattgc caaatctgag
     4261 caggagatcg gaaaggccac cgcaaagtac ttcttctaca gcaacatcat gaatttcttc
     4321 aagaccgaaa tcacccttgc aaacggtgag atccggaaga ggccgctcat cgagactaat
     4381 ggggagactg gcgaaatcgt gtgggacaag ggcagagatt tcgctaccgt gcgcaaagtg
     4441 ctttctatgc ctcaagtgaa catcgtgaag aaaaccgagg tgcaaaccgg aggcttttct
     4501 aaggaatcaa tcctccccaa gcgcaactcc gacaagctca ttgcaaggaa gaaggattgg
     4561 gaccctaaga agtacggcgg attcgattca ccaactgtgg cttattctgt cctggtcgtg
     4621 gctaaggtgg aaaaaggaaa gtctaagaag ctcaagagcg tgaaggaact gctgggtatc
     4681 accattatgg agcgcagctc cttcgagaag aacccaattg actttctcga agccaaaggt
     4741 tacaaggaag tcaagaagga ccttatcatc aagctcccaa agtatagcct gttcgaactg
     4801 gagaatgggc ggaagcggat gctcgcctcc gctggcgaac ttcagaaggg taatgagctg
     4861 gctctcccct ccaagtacgt gaatttcctc taccttgcaa gccattacga gaagctgaag
     4921 gggagccccg aggacaacga gcaaaagcaa ctgtttgtgg agcagcataa gcattatctg
     4981 gacgagatca ttgagcagat ttccgagttt tctaaacgcg tcattctcgc tgatgccaac
     5041 ctcgataaag tccttagcgc atacaataag cacagagaca aaccaattcg ggagcaggct
     5101 gagaatatca tccacctgtt caccctcacc aatcttggtg cccctgccgc attcaagtac
     5161 ttcgacacca ccatcgaccg gaaacgctat acctccacca aagaagtgct ggacgccacc
     5221 ctcatccacc agagcatcac cggactttac gaaactcgga ttgacctctc acagctcgga
     5281 ggggatgagg gagctgatcc aaaaaagaag agaaaggtag atccaaaaaa gaagagaaag
     5341 gtataaggat cctaactcga gattgaattg aattgaaatc gatagatcaa tttttttctt
     5401 ttctctttcc ccatccttta cgctaaaata atagtttatt ttattttttg aatatttttt
     5461 atttatatac gtatatatag actattattt atcttttaat gattattaag atttttatta
     5521 aaaaaaaatt cgctcctctt ttaatgcctt tatgcagttt ttttttccca ttcgatattt
     5581 ctatgtgctg agcagttaca gagatgttac gaaccactag tgcactgcag tacagtttag
     5641 cttgcctcgt ccccgccggg tcacccggcc agcgacatgg aggcccagaa taccctcctt
     5701 gacagtcttg acgtgcgcag ctcaggggca tgatgtgact gtcgcccgta catttagccc
     5761 atacatcccc atgtataatc atttgcatcc atacattttg atggccgcac ggcgcgaagc
     5821 aaaaattacg gctcctcgct ccagacctgc gagcagggaa acgctcccct cacagacgcg
     5881 ttgaattgtc cccacgccgc gcccctgtag agaaatataa aaggttagga tttgccactg
     5941 aggttcttct ttcatatact tccttttaaa atcttgctag gatacagttc tcacatcaca
     6001 tccgaacata aacaaaaatg ggtaaggaaa agactcacgt ttcgaggccg cgattaaatt
     6061 ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag
     6121 gtgcgacaat ctatcgattg tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg
     6181 gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac tggctgacgg
     6241 aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat gcatggttac
     6301 tcaccactgc gatccccggc aaaacagcat tccaggtatt agaagaatat cctgattcag
     6361 gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg attcctgttt
     6421 gtaattgtcc ttttaacagc gatcgcgtat ttcgtctggc tcaggcgcaa tcacgaatga
     6481 ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg cctgttgaac
     6541 aagtctggaa agaaatgcat aagcttttgc cattctcacc ggattcagtc gtcactcatg
     6601 gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt tgtattgatg
     6661 ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg aactgcctcg
     6721 gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg
     6781 atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaaagt aactgacaat
     6841 aaaaagattc ttgttttcaa gaacttgtca tttgtatagt ttttttatat tgtagttgtt
     6901 ctattttaat caaatgttag cgtgatttat attttttttc gcctcgacat catctgccca
     6961 gatgcgaagt taagtgcgca gaaagtaata tcatgcgtca atcgtatgtg aatgctggtc
     7021 gctatactgg agtatcacgt gctataaaaa taattataat ttaaattttt taatataaat
     7081 atataaatta aaaatagaaa gtaaaaaaag aaattaaaga aaaaatagtt tttgttttcc
     7141 gaagatgtaa aagactctag ggggatcgcc aacaaatact accttttatc ttgctcttcc
     7201 tgctctcagg tattaatgcc gaattgtttc atcttgtctg tgtagaaaac cacacacgaa
     7261 aatcctgtga ttttacattt tacttatcgt taatcgaatg tatatctatt taatctgctt
     7321 ttcttgtcta ataaatatat atgtaaagta cgctttttgt tgaaattttt taaacctttg
     7381 tttatttttt tttcttcatt ccgtaactct tctaccttct ttatttactt tctaaaatcc
     7441 aaatacaaaa cataaaaata aataaacaca gagtaaattc ccaaattatt ccatcattaa
     7501 aagatacgag gcgcgtgtaa gttacaggca agcgatccgt cccgagcggc cgcgattatc
     7561 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     7621 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     7681 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     7741 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcggg acccacgctc
     7801 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     7861 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     7921 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     7981 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     8041 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     8101 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     8161 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     8221 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     8281 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     8341 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     8401 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     8461 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     8521 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     8581 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctgt
     8641 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     8701 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     8761 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     8821 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     8881 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     8941 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     9001 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     9061 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     9121 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     9181 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     9241 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     9301 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     9361 catgttcttt cctgcgttat cccctgattc tgtggataac cgtgcggccg cccctgaatt
     9421 cgcatctaga ct
//
