LOCUS       sequence-38309-e         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Nechushtan A, Smith CL, Lamensdorf I, Yoon SH, Youle RJ
  TITLE     Bax and Bak coalesce into novel mitochondria-associated clusters 
            during apoptosis.
  JOURNAL   J Cell Biol. 2001 Jun 11. 153(6):1265-76.
  PUBMED    11402069
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2001 Jun 11. 153(6):1265-76."
COMMENT     Sequence Label: ECFP-Bak
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      44..53
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(50..52,53..55,56..766)
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(95..116)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(356..375)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     703..724
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gagctggttt agtgaaccgt cagatccgct agcgctaccg gtcgccacca tggtgagcaa
       61 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
      121 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
      181 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
      241 cctgacctgg ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt
      301 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
      361 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
      421 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
      481 caactacatc agccacaacg tctatatcac cgccgacaag cagaagaacg gcatcaaggc
      541 caacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
      601 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac
      661 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
      721 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtccg gactcagatc
      781 tcgagctatg gcttcggggc aaggcccagg tcctcccagg caggagtgc
//
