LOCUS       sequence-383124-        9858 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  vector for encoding a human codon-optimized dCas12Max-ABE driven by 
            CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and 
            mCherry driven by CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9858)
  AUTHORS   Zhang H, Kong X, Xue M, Hu J, Wang Z, Wei Y, Wang H, Zhou J, Zhang 
            W, Xu M, Shen X, Yin F, Ai Z, Huang G, Xia J, Song X, Li H, Yuan Y, 
            Li J, Zhong N, Zhang M, Zhou Y, Yang H
  TITLE     An engineered xCas12i with high activity, high specificity, and 
            broad PAM range.
  JOURNAL   Protein Cell. 2023 Jun 28;14(7):538-543. doi: 
            10.1093/procel/pwac052.
  PUBMED    37378659
REFERENCE   2  (bases 1 to 9858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Protein Cell"; date: "2023-06-28- 28"; volume: "14"; issue: "7"; 
            pages: "538-543"
COMMENT     Sequence Label: dCas12Max-ABE
FEATURES             Location/Qualifiers
     source          1..9858
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..249
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1..21
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     172..191
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        299..374
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        471..756
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     promoter        758..1035
                     /label=chicken beta-actin promoter
     intron          1036..1263
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     CDS             1317..1337
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1893..1913
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5178..5225
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(5253..5270)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5259..5466
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        5535..5838
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5839..6042
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5993..6013
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             6071..6781
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6216..6234)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     rep_origin      7336..7791
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7423..7442)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7633..7654
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7808..7827)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7927..7949
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7987..8005)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8073..8177
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8178..8246,8247..9038)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8396..8415)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9209..9797
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9698..9717
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
       61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
      121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
      181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
      241 cgaaacaccg agaaatgtgt ccccagttga cacagagacc gagaggtctc gtttttttgt
      301 tttagagcta gaaatagcaa gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg
      361 caccgagtcg gtgctttttt gttttagagc tagaaatagc aagttaaaat aaggctagtc
      421 cgtttttagc gcgtgcgcca attctgcaga caaatggctc tagaggtacc cgttacataa
      481 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
      541 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
      601 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
      661 ggtaaatggc ccgcctggca ttgtgcccag tacatgacct tatgggactt tcctacttgg
      721 cagtacatct acgtattagt catcgctatt accatggtcg aggtgagccc cacgttctgc
      781 ttcactctcc ccatctcccc cccctcccca cccccaattt tgtatttatt tattttttaa
      841 ttattttgtg cagcgatggg ggcggggggg gggggggggc gcgcgccagg cggggcgggg
      901 cggggcgagg ggcggggcgg ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc
      961 gcgctccgaa agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga
     1021 agcgcgcggc gggcgggagt cgctgcgcgc tgccttcgcc ccgtgccccg ctccgccgcc
     1081 gcctcgcgcc gcccgccccg gctctgactg accgcgttac tcccacaggt gagcgggcgg
     1141 gacggccctt ctcctccggg ctgtaattag ctgagcaaga ggtaagggtt taagggatgg
     1201 ttggttggtg gggtattaat gtttaattac ctggagcacc tgcctgaaat cacttttttt
     1261 caggttggac cggtgccacc atgaaacgga cagccgacgg aagcgagttc gagtcaccaa
     1321 agaagaagcg gaaagtctct gaggtggagt tttcccacga gtactggatg agacatgccc
     1381 tgaccctggc caagagggca cgggatgaga gggaggtgcc tgtgggagcc gtgctggtgc
     1441 tgaacaatag agtgatcggc gagggctgga acagagccat cggcctacac gacccaacag
     1501 cccatgccga aattatggcc ctgagacaag gcggcctggt catgcagaac tacagactga
     1561 ttgacgccac cctgtacgtg acattcgagc cttgcgtgat gtgcgctggc gccatgatcc
     1621 actctaggat cggccgcgtg gtgtttggag tgagaaattc taaaagaggc gccgcaggct
     1681 ccctgatgaa cgtgctgaac taccccggca tgaatcaccg cgtcgaaatt accgagggaa
     1741 tcctggcaga tgaatgtgcc gccctgctgt gcgacttcta tcggatgcct agacaggtgt
     1801 tcaatgctca gaagaaggcc cagagttcca tcaactccgg aggatctagc ggaggctcca
     1861 agagaaccgc tgatggctca gagtttgaga gcccaaaaaa gaaaagaaaa gtctctggcg
     1921 gaagctccgg gggctctatg agttctgatg tggtgcggcc ttataacaca aagctgctcc
     1981 cagataacag aaagcacaat atgttcctgc agaccttcaa gcggctgaac agcatctctc
     2041 tgaaccactt cgacctgctg atctgcctgt acgctgcaat caccaacaag aaggccgagg
     2101 aatacaagtc tgaaaaggaa gcccacgtga ccgccgatag cctgtgtgcc atcaattggt
     2161 tcagacccat gagcaagaga tacagcaaat acgccaccac caccttcaac atgttagaac
     2221 tgtttaagga gtacagcggc cacgagcctg atgcctattc caagaactac ctgatgagca
     2281 atatcgacag cgacagattc gtgtgggtgg attgtaggaa gttcgctaag gactttgcct
     2341 atcagatgga actgggtttc cacgagttca ccgtgttggc cgaaaccctg ctggctaatt
     2401 ctatcctggt gctgaacgag agcaccaagg ccaattgggc ttggggaacc gtgtctgccc
     2461 tgtacggcgg cggagataag gaggacagca cactgaagag caagattctg ctggccttcg
     2521 tggacgccct gaacaaccac gagctgaaaa caaagagaga aatcttgaat caagtgtgtg
     2581 aatctctgaa ataccagagc taccaggaca tgtacgtgga ttttagaagc gtggttgacg
     2641 aaaacggcaa caagaagtct cctagaggct ctatgcctat cgtgaccaag ttcgagacag
     2701 acgacctgat cagcgacaac caaagaaagg ccatgatcag caacttcact aagaacgccg
     2761 ctgccaaggc agctaagaaa cctatccctt acttggaccg cctgaaggag cacatggtgt
     2821 ccctgtgcga cgagtacaat gtgtatgcct gggccgcggc catcacaaac agcaacgccg
     2881 acgtgaccgc ccggaatacc agaaacctga cattcatcgg cgaacagaac agcagacgaa
     2941 agagactgag cgtgctgcag acaacaacca acgagaaggc taaggacatc ctgaacaaga
     3001 tcaacgacaa cctgattcag gaggtgcggt acacccctgc ccctaagcac ctgggcagag
     3061 atctggccaa cctgtttgat acactgaagg aaaaggacat caacaacatc gagaacgaag
     3121 aagagaaaca gaacgtgatc aatgactgta tcgagcagta cgtggacgat tgcagaagcc
     3181 tcaaccggaa ccccatcgca gccctcctga agcacatctc taggtactac gaggatttca
     3241 gcgccaagaa tttcctggac ggcgccaagc tgaacgtgct gactgaggtg gtgaaccggc
     3301 agaaggccca ccccaccatc tggagcgaga aggcttacac ctggatcagc aagttcgaca
     3361 agaaccggag acaggccaac agcagcctgg tcggatgggt tgtgcccccc gaggaggtgc
     3421 acaaggagaa aatcgccgga cagcagagca tgatgtgggt gaccctcacc ctgctggacg
     3481 acggcaagtg ggtcaaacat cacatcccct tcagcgacag cagatactac agcgaagtgt
     3541 acgcctacaa ccctaatctg ccttatctgg acggaggcat cccaagacag agcaagttcg
     3601 gcaacaaacc aacaaccaac ctgacagccg agtcccaggc cctcctggct aattctaagt
     3661 acaagaaagc caacaagagc ttcctgcggg ctaaagagaa tgccacacac aacgtgcggg
     3721 tgtcccctaa cacctctctg tgcattagac tgctgaagga cagcgccgga aaccagatgt
     3781 tcgacaaaat cggcaacgtg ctcttcggca tgcagatcaa ccacaagatc accgtgggaa
     3841 aacctaacta caagatcgag gtgggcgaca gattcctggg cttcgatcag aaccagagcg
     3901 agaaccacac ctacgccgtg ctgcagagag tgtccgagag cagtcacgac acccaccact
     3961 ttaacggctg ggacgtgaag gtgctggaaa agggcaaagt gaccagcgat gtgatcgtgc
     4021 gggacgaggt ctacgaccaa ctgtcttacg agggcgtccc ctacgatagc agcaagttcg
     4081 ccgagtggcg ggacaagcgc agaagatttg tgcttgagaa cctgagcatc cagctggaag
     4141 agggcaagac cttcctgaca gagttcgaca agctgaataa ggacagcctg taccgctgga
     4201 acatgaacta cctgaaactg ctgagaaagg ccatccgggc cggaggcaaa gagttcgcca
     4261 agatcgctaa gacagagatc ttcgagctgg cggtggaaag attcggccct attaacctgg
     4321 gcagcctgtc ccagatcagc cttaagatga ttgcctcctt taagggcgtg gtccagtcct
     4381 acttctccgt gagcggctgc gtggatgatg cctccaaaaa ggcccatgat tctatgctgt
     4441 tcacatttat gtgcgccgcc gaagaaaagc ggaccaacaa gagagaagaa aagaccaaca
     4501 gagccgccag ctttatcctg caaaaagcct acctgcatgg ctgcaagatg atcgtgtgcg
     4561 aggacgacct tcctgtggcc gacggcaaga caggcaaagc ccagaatgcc gaccggatgg
     4621 actggtgcgc cagagccctg gccaagaagg tgaacgacgg ctgtgttgcc atgagcatct
     4681 gctacagagc tatccctgcc tacatgagca gccaccagga cccctttgtg cacatgcagg
     4741 ataagaaaac cagcgtgctg cggcctagat tcatggaagt taataaggat agcatcagag
     4801 actaccacgt ggcgggcctg agaagaatgc tgaacagcaa gagtgacgct ggcaccagtg
     4861 tttattaccg gcaagctgcc ctgcatttct gcgaagccct gggcgtgagc cctgaactgg
     4921 tgaaaaacaa gaaaacccac gccgccgaac tgggcaagca catgggcagc gctatgctga
     4981 tgccctggag aggcggtaga gtgtacatcg ccagcaaaaa gctgacctcc gatgccaaat
     5041 cagtgaagta ctgcggcgag gatatgtggc agtaccacgc cgccgagatc gccgctgtta
     5101 acatcgccat gtatgaggtg tgctgccaga ccggcgcttt cggaaagaaa cagaaaaaat
     5161 cggacgagct gcctggaaaa aggccggcgg ccacgaagaa ggccggccag gcaaagaaga
     5221 agaagtaaga attcctagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc
     5281 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     5341 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     5401 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagagaata gcaggcatgc
     5461 tggggagcgg ccgctagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
     5521 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
     5581 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
     5641 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
     5701 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     5761 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     5821 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
     5881 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
     5941 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
     6001 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctggtttagt gaaccgtcag
     6061 atccgctagc atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat
     6121 gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga
     6181 gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg
     6241 ccccctgccc ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta
     6301 cgtgaagcac cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa
     6361 gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc
     6421 cctgcaggac ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga
     6481 cggccccgta atgcagaaga aaaccatggg ctgggaggcc tcctccgagc ggatgtaccc
     6541 cgaggacggc gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca
     6601 ctacgacgct gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc
     6661 ctacaacgtc aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga
     6721 acagtacgaa cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagta
     6781 aggatccggg atgcagaaat tgatgatcta ttaaacaata aagatgtcca ctaaaatgga
     6841 agtttttcct gtcatacttt gttaagaagg gtgagaacag agtacctaca ttttgaatgg
     6901 aaggattgga gctacggggg tgggggtggg gtgggattag ataaatgcct gctctttact
     6961 gaaggctctt tactattgct ttatgataat gtttcatagt tggatatcat aatttaaaca
     7021 agcaaaacca aattaagggc cagctcattc ctcccactca tgatctatag atctatagat
     7081 ctctcgtggg atcattgttt ttctcttgat tcccactttg tggttctaag tactgtggtt
     7141 tccaaatgtg tcagtttcat agcctgaaga acgagatcag cagcctctgt tccacataca
     7201 cttcattctc agtattgttt tgccaagttc taattccatc agaagctggt cgacctgcag
     7261 gggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgt
     7321 caaagcaacc atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     7381 cgcgcagcgt gaccgctaca cttgccagcg ccttagcgcc cgctcctttc gctttcttcc
     7441 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     7501 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg
     7561 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     7621 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaactct atctcgggct
     7681 attcttttga tttataaggg attttgccga tttcggtcta ttggttaaaa aatgagctga
     7741 tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt ttatggtgca
     7801 ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac
     7861 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     7921 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac
     7981 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     8041 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     8101 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     8161 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     8221 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     8281 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     8341 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     8401 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     8461 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     8521 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     8581 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     8641 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     8701 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     8761 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     8821 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     8881 ccggtgagcg tggaagccgc ggtatcattg cagcactggg gccagatggt aagccctccc
     8941 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     9001 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     9061 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     9121 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     9181 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     9241 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     9301 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     9361 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     9421 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     9481 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     9541 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     9601 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     9661 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     9721 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     9781 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     9841 ggccttttgc tcacatgt
//
