LOCUS       sequence-383138-       10878 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pBAD322-MycaIDCas-lacZsp5, arapBAD promoter expressing type I-D 
            Cascade and one spacer array of McCAST (Tn7575), the single spacer 
            is lacZ spacer 5..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10878)
  AUTHORS   Hsieh SC, Peters JE
  TITLE     Discovery and characterization of novel type I-D CRISPR-guided 
            transposons identified among diverse Tn7-like elements in 
            cyanobacteria.
  JOURNAL   Nucleic Acids Res. 2023 Jan 25;51(2):765-782. doi: 
            10.1093/nar/gkac1216.
  PUBMED    36537206
REFERENCE   2  (bases 1 to 10878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2023-01-25- 25"; volume: "51"; issue: 
            "2"; pages: "765-782"
COMMENT     Sequence Label: pOPO717
FEATURES             Location/Qualifiers
     source          1..10878
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(426..1304)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1331..1615
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1538..1557
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     primer_bind     complement(7654..7671)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      7824..7910
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      8002..8029
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        8048..8139
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8140..8208,8209..9000)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8358..8377)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9042..9497
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9129..9148)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9339..9360
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     rep_origin      9608..10196
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10097..10116
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10350..10367
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    10382..10522
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(10448..10467)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10608..10630
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(10624..10815)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg
       61 taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca
      121 gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg
      181 cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg
      241 atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac
      301 tgtgataaac taccgcatta aagctagctt atcgatgcat aatgtgcctg tcaaatggac
      361 gaagcaggga ttctgcaaac cctatgctac tccgtcaagc cgtcaattgt ctgattcgtt
      421 accaattatg acaacttgac ggctacatca ttcacttttt cttcacaacc ggcacggaac
      481 tcgctcgggc tggccccggt gcatttttta aatacccgcg agaaatagag ttgatcgtca
      541 aaaccaacat tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa aagcagcttc
      601 gcctggctga tacgttggtc ctcgcgccag cttaagacgc taatccctaa ctgctggcgg
      661 aaaagatgtg acagacgcga cggcgacaag caaacatgct gtgcgacgct ggcgatatca
      721 aaattgctgt ctgccaggtg atcgctgatg tactgacaag cctcgcgtac ccgattatcc
      781 atcggtggat ggagcgactc gttaatcgct tccatgcgcc gcagtaacaa ttgctcaagc
      841 agatttatcg ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt aatgatttgc
      901 ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa ccccgtattg
      961 gcaaatattg acggccagtt aagccattca tgccagtagg cgcgcggacg aaagtaaacc
     1021 cactggtgat accattcgcg agcctccgga tgacgaccgt agtgatgaat ctctcctggc
     1081 gggaacagca aaatatcacc cggtcggcaa acaaattctc gtccctgatt tttcaccacc
     1141 ccctgaccgc gaatggtgag attgagaata taacctttca ttcccagcgg tcggtcgata
     1201 aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag atgggcatta
     1261 aacgagtatc ccggcagcag gggatcattt tgcgcttcag ccatactttt catactcccg
     1321 ccattcagag aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt
     1381 tactggctct tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa
     1441 agcgggacca aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa
     1501 gtccacattg attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata
     1561 agattagcgg atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg
     1621 tttttttggg ctagcaggag gaattcacca tgaagccctt actccagact cttctacttc
     1681 aaactctacc gtctgacatt gatccagtat tgctggctta tgttgagact gtattacctg
     1741 ctgtagaaca ggagttcggt ctgattccag tttctaacga tgttagtcaa actgagcaaa
     1801 gcttgctggt tcacattctc aatagtttgc tgactgcctg gaacttgttc ccgttcctat
     1861 cctcaaaatt ggcgctatca gacaacgaga aacgattgct ttgcttgagt cttatctttc
     1921 aaaatgacaa cagtaagtgc cagagccgag agaataattc gcctcctacg cttggtgtta
     1981 atgaaaccct aaaacagtac cgagcatttg gagacaaact aaatctggcg gcgttctgga
     2041 ctgagtggcg tgagtatttg acagatatag tttatttaac ccaaagcacc caaccgcatg
     2101 ctttgtcaat caagaagact gctctcaaaa ttcctgctaa tcgcctagag ttgcctctac
     2161 gtccattgct tctgtttggg gcgatcgctg cccagcttac tgatcccgct gatattgtca
     2221 tcaaaccgga aggcaatgtc ttaaaagaat gcttgaaacg tctgggtgtt cagcatcaac
     2281 tcacttatca tcgagtacgc gcctgcacag gactcttaac tgagggcatc cacaatgcca
     2341 ttatgcgttt tgtacaacct ctcgactggc aatccattct gttctttgcc caagggatcg
     2401 tttatttggt tccctctgac agcaaactgc ttgattgctc aaaactacaa acctttatct
     2461 gggagcagat tagtgagcag ttagcaatca gtatgcttcg aggaaaagtt ggtttcaagc
     2521 gagatggcaa agggatgaaa gctgcgcctc aaacgctgga gctattctca ccagctcaat
     2581 tcattcggaa tctacccact gtcattaatg cctgtgtcaa gaatgagacg gaacctgcaa
     2641 cacctaaacg actggcaaat cttgaccttg atccaacaga tcgagaattt ttagcttgtg
     2701 gggcggatct aagaagcgat cgcattgcag aatttatcct tttcttgcag aagaagtttt
     2761 ttgggactaa tcctgaattt atcgatcgag ttctcgacca actgggcatt caatctgcca
     2821 tcactccaga acagactcaa atcttctcaa gcggggtaaa taggggatgg tatcacgcag
     2881 ccgcttatta cgtcgccagt aaccctcact tagactcaga tgatgtaacg atcaagcttc
     2941 aggagatcgc caatcaacta gtggactggg ctgaagagaa agaacttttt ccacctcaaa
     3001 tcagtccgac tcgtgaggtt ttcaatcaat acttagctca gtacttggaa ttcccaaggt
     3061 ggaataattc agcgccatcc tttcaacatg aattggcaac gtatgtagcg gcaaaaacaa
     3121 aagccgctaa acaaccaatt tgctccctca gttccagtga atttgactca gaagaccagc
     3181 tagattctgt tgtgctattc aagcctcagc agtatagcaa caagaatccc ctcggagggt
     3241 ggcaactgaa gcgaggaatt tctaaactct ggtcattaga gatgcttctg cggcagtctt
     3301 tctggtcagt ttccgcaggc aagctggaag agcgacaacc tgtctttctg tacttttttc
     3361 ccagtttggt tcattcccct caggtcgcaa aagcgattcg tatcctagtc aatcaactta
     3421 aacgagtcaa tttgtggaaa gtttgtcgat tctggcaggt acacgggatg aatgctaata
     3481 ccctgcgctt ctactcttgg ctgaagtctg atcccagacc agacgataat ggtaaacgtg
     3541 atctcccttt cctagcaatc acttacacca ccacagctgg taaaacgacg acacaggctt
     3601 ggattgaacc tgcttttttg gtattggcgc tctcacttgt gctgggcatt aaagtagtcg
     3661 ctacggcaag tccagtacca ctttataaca ggagtagcga gttccccgaa acagtgaaga
     3721 tagacggacc tgcgagcttc tggagcctgt tgggtttgcc aaccagcatt cggctggaag
     3781 agaagatcca gggacgggtc aaacacctga atgattggct cgatcgccta ctaattgctt
     3841 acagtattca ccttgactgt caagcgaatc cccctgactc tcgttggcaa ttgtttcccg
     3901 gtacagtgca ggctataaca actgatgtct cagcgatttt tagccttgct cattcgcatt
     3961 ttcaaagtct ttcggatgac agagttcaac gatactggca ctttgctcaa gtatgggcag
     4021 caggagattc aaacatggaa cagcaattgg agctaactca gcgactggtc caggaatatc
     4081 gcgcgttcta ccaagttcat gtagcgaaag caacccacgc gatcgtgctc ccgctgctaa
     4141 aggttttaga ggagattctt tcaactccta gtgatctgcc aattgaaact ctgatattac
     4201 agacatcggg acggttacat gacgctttga agcgacagga accctacaaa cgccccctga
     4261 ttatggatca gtctttatca attgagattc gtgtcaataa cgagattcag gcaattcaac
     4321 gatttgtcac cacttgcatc aatgatttat ttttagctca gtacaaaggc gatcgcgccc
     4381 tactgcaaga acaccgtaac cgaattaaat cgggtgcaga gtttgcgtat cgtttactag
     4441 ctatgcagga gccaaaacca aaaacgaaaa agcaagaaat tgaaggagca cagatatgag
     4501 tgtgttagaa gaattgaaac ctcaattcca ttcggctttt ccccgtctgg catccgggaa
     4561 gtatgttcat gtcttcctgg tacgtcacag tcaatccttt cccgtgtttc aaacagatgg
     4621 tgtcctcaat acggctcgaa ctcaagctgg cttggattcc aaagatgata aatctaggca
     4681 atcgatcaac cgattggtca tgttcaagcg caagcaagta acacccgaac gactggttgg
     4741 acgacaactg ctacgatcgc taggactaac cagtgctgta gaaggcgaag tcaacttttg
     4801 cgaatacaac agtcagaaat cgtgcaagaa atgcccagac tgtattatct acggattcgg
     4861 ggttggagac aaaggtgcag aacgctcgaa agtctactca gactcagcgt tctcgctcag
     4921 tccttatgaa acttcccacc gcagtttcac cttcaatgcg ccgtcagaaa atggcacgat
     4981 gagtgaaaag ggagaactgc gaagttcgat caacgatcta gaccatgtcc tgccggaagt
     5041 gatgtttcct agtgttgaaa cgctgcgtga tcccacattt gaggggttta tttatgtgct
     5101 gggcaacctg attcgcacgc gccgctatgg aggtcaagaa tcccgaaccg ggacgatgat
     5161 gaatcacatt gttggtattg ccttttgcga tggtgaagtt ttcagcaact tgtatttcac
     5221 acaagcgcta tatgatgcct tgaatgcaaa gggggatatt tgcgtgacga tcgaggactt
     5281 tcgacatgag gcagcaaggg tggcaaaaaa ctgtctgaaa caggagccag ttcgcagcat
     5341 cacaattttt ggggctgaac tcgatgcctt gctcgaagaa gtgacgggca tctatcagga
     5401 tgaagttaaa cagcgcctta ttatgaccac gctttaccaa cagacccaga tctacagtga
     5461 aagctacgga gcaatttttc ctgagaagaa gaaagaggaa aagaaaaaag cacagcaagg
     5521 ctcttcgatc tgagggatgg tggaataatg gttttctatt tttgccattt aaccttgcat
     5581 gacaatgttt tctttgcctc acgggagatg ggaactctct atgagacaga aaaatacctg
     5641 cacaattggg cacttagcta tgcgctattt gaaagcgcct acattcctag accttatcga
     5701 ttggaagggg cagtcgccca gcgtcctacg tatctagaga ttgatagtga gcaaaacctg
     5761 ttacatctga atcagcaagg aatttatgtt tttcccgccc tgccgcttca gtggacatat
     5821 caggtcaata cattcaatgt gtctcaagcc ttctaccatc aaaaatcaag acggttgggc
     5881 tcgggaggac atattctcaa ttacccaatc agctatggac gggcaaagga actcgcagtt
     5941 ggcagcaagt acagaacctt catcattgct cctgccaaaa cgagaatccc ccgctggata
     6001 agactaggca aatggtcagc gaaaattcga gtcgagatca tccccattcc taaaagtgga
     6061 attcaaaggg cagcaggcga gtatgtttct gagcatcccc tcaatcctgt agatctctct
     6121 aaaacgaccc gattattgct gtataaccgg attgtcatgc caccttctag tctggtgagc
     6181 caggcaaaat tgttgggtga ctattgggag atctcaaaac cagctcagtg ggctaaattg
     6241 tctgagtggt caagcttaga gaatttgcct gaaacgattt gtttgccaag aggagttgct
     6301 tatggagcta acactctcgt taccacacct tgaagcgaca gcattgcaga cattggtgat
     6361 taaactcggg gctgcgggca atggagattt ttccttatcc tgtagccgag cacttcatgc
     6421 tcaagttctg aaatggtttc agttaggaca accacaaatt tctcaagcaa ttcatgatag
     6481 tcaggagtct cccatcagcc tctcaggact aatgggcaag cgctttggaa gagttgtgca
     6541 ggagggagat gaattctact ttcgggttgg gttactgaat ggaaatctgc ttgaaccgtt
     6601 gttgaaaggg ctagagcagt gggaaagtca agtagtgctc ctttctaaat ttccttttgt
     6661 tatcaaagat atcaatatgt tgccaggtag ccactttctg gtcggctctt cagactacac
     6721 tctgcttgcc caaatgcctt cagacgcaaa agcgctaaca ctaaggttcc tctctccgac
     6781 tagctttaag ctcgctcaaa atcggtacat tcaaccagtt ccattgcccg attcagtttt
     6841 tggaagttta cagcgtcgct ggaatacctt tgctcccaaa gagttacggt ttcccaaaac
     6901 tcaatgggcg ggattgattt gtgactttga aattaaatcc cagcggctaa atctggacag
     6961 gacagttgag ctgggttccg ttggatgggt tagataccag tttcccgatt tgcaacaagc
     7021 tagaattgca aacgttttgg ctcactttgc cttcttctca ggagtaggac gcaaaacggc
     7081 aatgggcatg ggattgactc tacttaaagg aacaactcca agttgagtgt gaccaaaact
     7141 agggcataga cttgtggcag actgcagcaa gttccaaatc ttcagaaggt ttgctaaaac
     7201 tattaaaatg cttaagactc aaggctctac tcatttctca tgatataaat aagttcaata
     7261 attcctggct ttaagggctg aatcttaact ttcctagaat cagctttgcc aaagacatgg
     7321 ttaagatttt gtcacggttg gctctgagta tgctaaaggc tcacgaaaca tcccccggtt
     7381 tgcgggactg aaaggcacta cgcgtactgt gagccagagt tgcccggcga actcgtgaag
     7441 catctcccga tttgcgggat tgtaaaatta tacgacttca cccataggtt gaactttaag
     7501 tcgtgatcag aacacaacac gcgagaagaa ccagaatggc aagtattact gcgatcgctt
     7561 agtggagtgg attctaggta cccggggatc ctctagagtc gacctgcagg catgcaagct
     7621 tggctgtttt ggcggatgag agaagatttt cagcctgata cagattaaat cagaacgcag
     7681 aagcggtctg ataaaacaga atttgcctgg cggcagtagc gcggtggtcc cacctgaccc
     7741 catgccgaac tcagaagtga aacgccgtag cgccgatggt agtgtggggt ctccccatgc
     7801 gagagtaggg aactgccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
     7861 ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat ccgccgggag
     7921 cggatttgaa cgttgcgaag caacggcccg gagggtggcg ggcaggacgc ccgccataaa
     7981 ctgccaggca tcaaattaag cagaaggcca tcctgacgga tggccttttt gcgtttctac
     8041 aaactctttt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     8101 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     8161 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     8221 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     8281 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     8341 agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag
     8401 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     8461 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     8521 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     8581 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     8641 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     8701 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     8761 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     8821 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     8881 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     8941 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     9001 ctgtcagacc aagtttactc atatatactt tagattgatt tacgcgccct gtagcggcgc
     9061 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     9121 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     9181 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     9241 ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     9301 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     9361 aacaacactc aaccctatct cgggctattc ttttgattta taagggattt tgccgatttc
     9421 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     9481 attaacgttt acaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     9541 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     9601 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     9661 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     9721 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     9781 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     9841 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     9901 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     9961 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
    10021 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
    10081 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
    10141 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
    10201 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
    10261 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
    10321 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
    10381 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca
    10441 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct
    10501 acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg
    10561 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
    10621 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc
    10681 agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag
    10741 tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt
    10801 ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat
    10861 accgatgaaa cgagagag
//
