LOCUS       sequence-383141-       10169 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10169)
  AUTHORS   Fasano CA, Dimos JT, Ivanova NB, Lowry N, Lemischka IR, Temple S
  TITLE     shRNA knockdown of Bmi-1 reveals a critical role for p21-Rb pathway 
            in NSC self-renewal during development.
  JOURNAL   Cell Stem Cell. 2007 Jun 7. 1(1):87-99.
  PUBMED    18371338
REFERENCE   2  (bases 1 to 10169)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2007 Jun 7. 1(1):87-99."
COMMENT     Sequence Label: p21 shRNA2
FEATURES             Location/Qualifiers
     source          1..10169
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    417..538
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(454..473)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     508..527
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(587..603)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(587..603)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(600..622)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    611..627
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(635..641,642..659,660..665))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    680..701
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(818..835)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(989..1577)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1069..1088)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1748..2539,2540..2608))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2371..2390
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2609..2713)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(2788..2807)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2979..3358
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3360..3558
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3513..3533
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             3576..3756
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3803..3928
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4421..4654
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4839..4883
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5181..5298
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        complement(5442..5656)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     complement(5502..5521)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     promoter        5685..6084
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     6733..6752
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      6859..6868
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6865..6867,6868..6870,6871..7584)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6865..6867,6868..6870,6871..7581)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6910..6931)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7171..7190)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7518..7539
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    7611..8199
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7664..7684)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8082..8093)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8202..8218)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8203..8219)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8724..8904
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8921..8938)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8927..9151
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9197..9625
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9284..9303)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9494..9515
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9634..9654)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9639..9968
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9819..9954
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9881..9900
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10016..10063
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             join(10082..10169,1..287)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
ORIGIN
        1 cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca
       61 tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg
      121 gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct
      181 ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg
      241 acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag
      301 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
      361 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact
      421 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
      481 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
      541 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
      601 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
      661 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
      721 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
      781 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
      841 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
      901 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
      961 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     1021 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     1081 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     1141 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     1201 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     1261 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     1321 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     1381 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     1441 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     1501 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     1561 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     1621 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     1681 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     1741 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     1801 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     1861 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     1921 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     1981 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     2041 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     2101 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     2161 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     2221 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     2281 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     2341 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     2401 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     2461 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     2521 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     2581 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     2641 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     2701 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc
     2761 tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     2821 gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc
     2881 tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt
     2941 tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta
     3001 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
     3061 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
     3121 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
     3181 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
     3241 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
     3301 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
     3361 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
     3421 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
     3481 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
     3541 ggaggtctat ataagcagcg cgttttgcct gtactgggtc tctctggtta gaccagatct
     3601 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     3661 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     3721 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
     3781 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
     3841 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
     3901 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
     3961 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
     4021 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
     4081 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
     4141 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
     4201 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
     4261 gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
     4321 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
     4381 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
     4441 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
     4501 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
     4561 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
     4621 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
     4681 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
     4741 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
     4801 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
     4861 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
     4921 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
     4981 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
     5041 caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca
     5101 gagacagatc cattcgatta gtgaacggat cggcactgcg tgcgccaatt ctgcagacaa
     5161 atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg
     5221 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
     5281 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt ttggttaatt
     5341 aagaaaggat ccgaaagaat tcgcccttag atctctagac aaaaaaagta gcagttgtac
     5401 aaggatctct tgaatccttg tacaactgct actgggaaag agtggtctca tacagaactt
     5461 ataagattcc caaatccaaa gacatttcac gtttatggtg atttcccaga acacatagcg
     5521 acatgcaaat attgcagggc gccactcccc tgtccctcac agccatcttc ctgccagggc
     5581 gcacgcgcgc tgggtgttcc cgcctagtga cactgggccc gcgattcctt ggagcgggtt
     5641 gatgacgtca gcgttcttaa ttaacccgtg tcggctccag atctggcctc cgcgccgggt
     5701 tttggcgcct cccgcgggcg cccccctcct cacggcgagc gctgccacgt cagacgaagg
     5761 gcgcagcgag cgtcctgatc cttccgcccg gacgctcagg acagcggccc gctgctcata
     5821 agactcggcc ttagaacccc agtatcagca gaaggacatt ttaggacggg acttgggtga
     5881 ctctagggca ctggttttct ttccagagag cggaacaggc gaggaaaagt agtcccttct
     5941 cggcgattct gcggagggat ctccgtgggg cggtgaacgc cgatgattat ataaggacgc
     6001 gccgggtgtg gcacagctag ttccgtcgca gccgggattt gggtcgcggt tcttgtttgt
     6061 ggatcgctgt gatcgtcact tggtgagtag cgggctgctg ggctggccgg ggctttcgtg
     6121 gccgccgggc cgctcggtgg gacggaagcg tgtggagaga ccgccaaggg ctgtagtctg
     6181 ggtccgcgag caaggttgcc ctgaactggg ggttgggggg agcgcagcaa aatggcggct
     6241 gttcccgagt cttgaatgga agacgcttgt gaggcgggct gtgaggtcgt tgaaacaagg
     6301 tggggggcat ggtgggcggc aagaacccaa ggtcttgagg ccttcgctaa tgcgggaaag
     6361 ctcttattcg ggtgagatgg gctggggcac catctgggga ccctgacgtg aagtttgtca
     6421 ctgactggag aactcggttt gtcgtctgtt gcgggggcgg cagttatggc ggtgccgttg
     6481 ggcagtgcac ccgtaccttt gggagcgcgc gccctcgtcg tgtcgtgacg tcacccgttc
     6541 tgttggctta taatgcaggg tggggccacc tgccggtagg tgtgcggtag gcttttctcc
     6601 gtcgcaggac gcagggttcg ggcctagggt aggctctcct gaatcgacag gcgccggacc
     6661 tctggtgagg ggagggataa gtgaggcgtc agtttctttg gtcggtttta tgtacctatc
     6721 ttcttaagta gctgaagctc cggttttgaa ctatgcgctc ggggttggcg agtgtgtttt
     6781 gtgaagtttt ttaggcacct tttgaaatgt aatcatttgg gtcaatatgt aattttcagt
     6841 gttagactag taccggtcgc caccatggtg agcaagggcg aggagctgtt caccggggtg
     6901 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     6961 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     7021 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
     7081 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     7141 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     7201 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     7261 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     7321 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
     7381 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     7441 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
     7501 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     7561 ggcatggacg agctgtacaa gtaaagcaat tcgatatcaa gcttatcgat aatcaacctc
     7621 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     7681 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     7741 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     7801 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     7861 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     7921 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     7981 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
     8041 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     8101 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     8161 ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccgt cgacctcgag
     8221 acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg
     8281 cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt
     8341 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     8401 gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc
     8461 acacacaagg ctacttccct gattggcaga actacacacc agggccaggg atcagatatc
     8521 cactgacctt tggatggtgc tacaagctag taccagttga gcaagagaag gtagaagaag
     8581 ccaatgaagg agagaacacc cgcttgttac accctgtgag cctgcatggg atggatgacc
     8641 cggagagaga agtattagag tggaggtttg acagccgcct agcatttcat cacatggccc
     8701 gagagctgca tccggactgt actgggtctc tctggttaga ccagatctga gcctgggagc
     8761 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     8821 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     8881 agtcagtgtg gaaaatctct agcagaaacc cgctgatcag cctcgactgt gccttctagt
     8941 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     9001 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     9061 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     9121 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc
     9181 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     9241 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     9301 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     9361 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     9421 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     9481 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     9541 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     9601 atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa
     9661 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     9721 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     9781 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     9841 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     9901 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     9961 tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcag cacgtgttga
    10021 caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga ggaactaaac
    10081 catggccaag ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt
    10141 cgagttctgg accgaccggc tcgggttct
//
