LOCUS       sequence-383143-        9879 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pBAD322-MycaIDCas-Delta-Cas6-RGR-lacZs, arapBAD promoter expressing 
            type I-D Cascade of McCAST (Tn7575) without cas6 gene and ribozyme 
            flanked lacZ spacer 5..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9879)
  AUTHORS   Hsieh SC, Peters JE
  TITLE     Discovery and characterization of novel type I-D CRISPR-guided 
            transposons identified among diverse Tn7-like elements in 
            cyanobacteria.
  JOURNAL   Nucleic Acids Res. 2023 Jan 25;51(2):765-782. doi: 
            10.1093/nar/gkac1216.
  PUBMED    36537206
REFERENCE   2  (bases 1 to 9879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2023-01-25- 25"; volume: "51"; issue: 
            "2"; pages: "765-782"
COMMENT     Sequence Label: pOPO812
FEATURES             Location/Qualifiers
     source          1..9879
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      162..248
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      340..367
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        386..477
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(478..546,547..1338)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(696..715)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1380..1835
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1467..1486)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1677..1698
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     rep_origin      1946..2534
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2435..2454
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2688..2705
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2720..2860
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2786..2805)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2946..2968
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2962..3153)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(3642..4520)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        4547..4831
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     4754..4773
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     terminator      9567..9594
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        9601..9635
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     ncRNA           9647..9683
                     /product="self-cleaving hammerhead ribozyme (Ferre-D'Amare 
                     and Scott, 2010)"
                     /label=hammerhead ribozyme
                     /ncRNA_class="hammerhead_ribozyme"
     ncRNA           9727..9794
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     primer_bind     complement(join(9871..9879,1..9))
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
ORIGIN
        1 gattaaatca gaacgcagaa gcggtctgat aaaacagaat ttgcctggcg gcagtagcgc
       61 ggtggtccca cctgacccca tgccgaactc agaagtgaaa cgccgtagcg ccgatggtag
      121 tgtggggtct ccccatgcga gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc
      181 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctcctgagta
      241 ggacaaatcc gccgggagcg gatttgaacg ttgcgaagca acggcccgga gggtggcggg
      301 caggacgccc gccataaact gccaggcatc aaattaagca gaaggccatc ctgacggatg
      361 gcctttttgc gtttctacaa actcttttgt ttatttttct aaatacattc aaatatgtat
      421 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
      481 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
      541 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
      601 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
      661 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
      721 gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
      781 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
      841 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
      901 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
      961 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     1021 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     1081 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     1141 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     1201 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     1261 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     1321 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     1381 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     1441 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     1501 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     1561 tgctttacgg cacctcgacc ccaaaaaact tgatttgggt gatggttcac gtagtgggcc
     1621 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     1681 actcttgttc caaactggaa caacactcaa ccctatctcg ggctattctt ttgatttata
     1741 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     1801 cgcgaatttt aacaaaatat taacgtttac aatttaaaag gatctaggtg aagatccttt
     1861 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     1921 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     1981 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     2041 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     2101 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     2161 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     2221 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     2281 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     2341 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     2401 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     2461 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     2521 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     2581 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     2641 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     2701 gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
     2761 cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
     2821 atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc
     2881 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     2941 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca
     3001 gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc
     3061 cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc
     3121 catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct
     3181 gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga
     3241 tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg
     3301 gcgggaccag agaaaaatca ctcagggtca atgccagcta gcagcacgcc atagtgactg
     3361 gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag
     3421 cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat
     3481 ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc
     3541 tagcttatcg atgcataatg tgcctgtcaa atggacgaag cagggattct gcaaacccta
     3601 tgctactccg tcaagccgtc aattgtctga ttcgttacca attatgacaa cttgacggct
     3661 acatcattca ctttttcttc acaaccggca cggaactcgc tcgggctggc cccggtgcat
     3721 tttttaaata cccgcgagaa atagagttga tcgtcaaaac caacattgcg accgacggtg
     3781 gcgataggca tccgggtggt gctcaaaagc agcttcgcct ggctgatacg ttggtcctcg
     3841 cgccagctta agacgctaat ccctaactgc tggcggaaaa gatgtgacag acgcgacggc
     3901 gacaagcaaa catgctgtgc gacgctggcg atatcaaaat tgctgtctgc caggtgatcg
     3961 ctgatgtact gacaagcctc gcgtacccga ttatccatcg gtggatggag cgactcgtta
     4021 atcgcttcca tgcgccgcag taacaattgc tcaagcagat ttatcgccag cagctccgaa
     4081 tagcgccctt ccccttgccc ggcgttaatg atttgcccaa acaggtcgct gaaatgcggc
     4141 tggtgcgctt catccgggcg aaagaacccc gtattggcaa atattgacgg ccagttaagc
     4201 cattcatgcc agtaggcgcg cggacgaaag taaacccact ggtgatacca ttcgcgagcc
     4261 tccggatgac gaccgtagtg atgaatctct cctggcggga acagcaaaat atcacccggt
     4321 cggcaaacaa attctcgtcc ctgatttttc accaccccct gaccgcgaat ggtgagattg
     4381 agaatataac ctttcattcc cagcggtcgg tcgataaaaa aatcgagata accgttggcc
     4441 tcaatcggcg ttaaacccgc caccagatgg gcattaaacg agtatcccgg cagcagggga
     4501 tcattttgcg cttcagccat acttttcata ctcccgccat tcagagaaga aaccaattgt
     4561 ccatattgca tcagacattg ccgtcactgc gtcttttact ggctcttctc gctaaccaaa
     4621 ccggtaaccc cgcttattaa aagcattctg taacaaagcg ggaccaaagc catgacaaaa
     4681 acgcgtaaca aaagtgtcta taatcacggc agaaaagtcc acattgatta tttgcacggc
     4741 gtcacacttt gctatgccat agcattttta tccataagat tagcggatcc tacctgacgc
     4801 tttttatcgc aactctctac tgtttctcca tacccgtttt tttgggctag caggaggaat
     4861 tcaccatgaa gcccttactc cagactcttc tacttcaaac tctaccgtct gacattgatc
     4921 cagtattgct ggcttatgtt gagactgtat tacctgctgt agaacaggag ttcggtctga
     4981 ttccagtttc taacgatgtt agtcaaactg agcaaagctt gctggttcac attctcaata
     5041 gtttgctgac tgcctggaac ttgttcccgt tcctatcctc aaaattggcg ctatcagaca
     5101 acgagaaacg attgctttgc ttgagtctta tctttcaaaa tgacaacagt aagtgccaga
     5161 gccgagagaa taattcgcct cctacgcttg gtgttaatga aaccctaaaa cagtaccgag
     5221 catttggaga caaactaaat ctggcggcgt tctggactga gtggcgtgag tatttgacag
     5281 atatagttta tttaacccaa agcacccaac cgcatgcttt gtcaatcaag aagactgctc
     5341 tcaaaattcc tgctaatcgc ctagagttgc ctctacgtcc attgcttctg tttggggcga
     5401 tcgctgccca gcttactgat cccgctgata ttgtcatcaa accggaaggc aatgtcttaa
     5461 aagaatgctt gaaacgtctg ggtgttcagc atcaactcac ttatcatcga gtacgcgcct
     5521 gcacaggact cttaactgag ggcatccaca atgccattat gcgttttgta caacctctcg
     5581 actggcaatc cattctgttc tttgcccaag ggatcgttta tttggttccc tctgacagca
     5641 aactgcttga ttgctcaaaa ctacaaacct ttatctggga gcagattagt gagcagttag
     5701 caatcagtat gcttcgagga aaagttggtt tcaagcgaga tggcaaaggg atgaaagctg
     5761 cgcctcaaac gctggagcta ttctcaccag ctcaattcat tcggaatcta cccactgtca
     5821 ttaatgcctg tgtcaagaat gagacggaac ctgcaacacc taaacgactg gcaaatcttg
     5881 accttgatcc aacagatcga gaatttttag cttgtggggc ggatctaaga agcgatcgca
     5941 ttgcagaatt tatccttttc ttgcagaaga agttttttgg gactaatcct gaatttatcg
     6001 atcgagttct cgaccaactg ggcattcaat ctgccatcac tccagaacag actcaaatct
     6061 tctcaagcgg ggtaaatagg ggatggtatc acgcagccgc ttattacgtc gccagtaacc
     6121 ctcacttaga ctcagatgat gtaacgatca agcttcagga gatcgccaat caactagtgg
     6181 actgggctga agagaaagaa ctttttccac ctcaaatcag tccgactcgt gaggttttca
     6241 atcaatactt agctcagtac ttggaattcc caaggtggaa taattcagcg ccatcctttc
     6301 aacatgaatt ggcaacgtat gtagcggcaa aaacaaaagc cgctaaacaa ccaatttgct
     6361 ccctcagttc cagtgaattt gactcagaag accagctaga ttctgttgtg ctattcaagc
     6421 ctcagcagta tagcaacaag aatcccctcg gagggtggca actgaagcga ggaatttcta
     6481 aactctggtc attagagatg cttctgcggc agtctttctg gtcagtttcc gcaggcaagc
     6541 tggaagagcg acaacctgtc tttctgtact tttttcccag tttggttcat tcccctcagg
     6601 tcgcaaaagc gattcgtatc ctagtcaatc aacttaaacg agtcaatttg tggaaagttt
     6661 gtcgattctg gcaggtacac gggatgaatg ctaataccct gcgcttctac tcttggctga
     6721 agtctgatcc cagaccagac gataatggta aacgtgatct ccctttccta gcaatcactt
     6781 acaccaccac agctggtaaa acgacgacac aggcttggat tgaacctgct tttttggtat
     6841 tggcgctctc acttgtgctg ggcattaaag tagtcgctac ggcaagtcca gtaccacttt
     6901 ataacaggag tagcgagttc cccgaaacag tgaagataga cggacctgcg agcttctgga
     6961 gcctgttggg tttgccaacc agcattcggc tggaagagaa gatccaggga cgggtcaaac
     7021 acctgaatga ttggctcgat cgcctactaa ttgcttacag tattcacctt gactgtcaag
     7081 cgaatccccc tgactctcgt tggcaattgt ttcccggtac agtgcaggct ataacaactg
     7141 atgtctcagc gatttttagc cttgctcatt cgcattttca aagtctttcg gatgacagag
     7201 ttcaacgata ctggcacttt gctcaagtat gggcagcagg agattcaaac atggaacagc
     7261 aattggagct aactcagcga ctggtccagg aatatcgcgc gttctaccaa gttcatgtag
     7321 cgaaagcaac ccacgcgatc gtgctcccgc tgctaaaggt tttagaggag attctttcaa
     7381 ctcctagtga tctgccaatt gaaactctga tattacagac atcgggacgg ttacatgacg
     7441 ctttgaagcg acaggaaccc tacaaacgcc ccctgattat ggatcagtct ttatcaattg
     7501 agattcgtgt caataacgag attcaggcaa ttcaacgatt tgtcaccact tgcatcaatg
     7561 atttattttt agctcagtac aaaggcgatc gcgccctact gcaagaacac cgtaaccgaa
     7621 ttaaatcggg tgcagagttt gcgtatcgtt tactagctat gcaggagcca aaaccaaaaa
     7681 cgaaaaagca agaaattgaa ggagcacaga tatgagtgtg ttagaagaat tgaaacctca
     7741 attccattcg gcttttcccc gtctggcatc cgggaagtat gttcatgtct tcctggtacg
     7801 tcacagtcaa tcctttcccg tgtttcaaac agatggtgtc ctcaatacgg ctcgaactca
     7861 agctggcttg gattccaaag atgataaatc taggcaatcg atcaaccgat tggtcatgtt
     7921 caagcgcaag caagtaacac ccgaacgact ggttggacga caactgctac gatcgctagg
     7981 actaaccagt gctgtagaag gcgaagtcaa cttttgcgaa tacaacagtc agaaatcgtg
     8041 caagaaatgc ccagactgta ttatctacgg attcggggtt ggagacaaag gtgcagaacg
     8101 ctcgaaagtc tactcagact cagcgttctc gctcagtcct tatgaaactt cccaccgcag
     8161 tttcaccttc aatgcgccgt cagaaaatgg cacgatgagt gaaaagggag aactgcgaag
     8221 ttcgatcaac gatctagacc atgtcctgcc ggaagtgatg tttcctagtg ttgaaacgct
     8281 gcgtgatccc acatttgagg ggtttattta tgtgctgggc aacctgattc gcacgcgccg
     8341 ctatggaggt caagaatccc gaaccgggac gatgatgaat cacattgttg gtattgcctt
     8401 ttgcgatggt gaagttttca gcaacttgta tttcacacaa gcgctatatg atgccttgaa
     8461 tgcaaagggg gatatttgcg tgacgatcga ggactttcga catgaggcag caagggtggc
     8521 aaaaaactgt ctgaaacagg agccagttcg cagcatcaca atttttgggg ctgaactcga
     8581 tgccttgctc gaagaagtga cgggcatcta tcaggatgaa gttaaacagc gccttattat
     8641 gaccacgctt taccaacaga cccagatcta cagtgaaagc tacggagcaa tttttcctga
     8701 gaagaagaaa gaggaaaaga aaaaagcaca gcaaggctct tcgatctgag ggatggtgga
     8761 ataatggttt tctatttttg ccatttaacc ttgcatgaca atgttttctt tgcctcacgg
     8821 gagatgggaa ctctctatga gacagaaaaa tacctgcaca attgggcact tagctatgcg
     8881 ctatttgaaa gcgcctacat tcctagacct tatcgattgg aaggggcagt cgcccagcgt
     8941 cctacgtatc tagagattga tagtgagcaa aacctgttac atctgaatca gcaaggaatt
     9001 tatgtttttc ccgccctgcc gcttcagtgg acatatcagg tcaatacatt caatgtgtct
     9061 caagccttct accatcaaaa atcaagacgg ttgggctcgg gaggacatat tctcaattac
     9121 ccaatcagct atggacgggc aaaggaactc gcagttggca gcaagtacag aaccttcatc
     9181 attgctcctg ccaaaacgag aatcccccgc tggataagac taggcaaatg gtcagcgaaa
     9241 attcgagtcg agatcatccc cattcctaaa agtggaattc aaagggcagc aggcgagtat
     9301 gtttctgagc atcccctcaa tcctgtagat ctctctaaaa cgacccgatt attgctgtat
     9361 aaccggattg tcatgccacc ttctagtctg gtgagccagg caaaattgtt gggtgactat
     9421 tgggagatct caaaaccagc tcagtgggct aaattgtctg agtggtcaag cttagagaat
     9481 ttgcctgaaa cgatttgttt gccaagagga gttgcttatg gagctaacac tctcgttacc
     9541 acaccttaac taaaaaaaat caaactggct caccttcggg tgggcctttt tgcgtttata
     9601 ttgacagcta gctcagtcct aggtataatg ctagctactt ttcagtctga tgagtccgtg
     9661 aggacgaaac gagtaagctc gtcactgaaa ggcactacgc gtactgtgag ccagagttgc
     9721 ccggcgggcc ggcatggtcc cagcctcctc gctggcgccg gctgggcaac atgcttcggc
     9781 atggcgaatg ggacgtaccc ggggatcctc tagagtcgac ctgcaggcat gcaagcttgg
     9841 ctgttttggc ggatgagaga agattttcag cctgataca
//
