LOCUS       sequence-383144-        9872 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pBAD322-MycaIDCas-Delta-Cas6-RGR-PaqCI, arapBAD promoter expressing 
            type I-D Cascade of McCAST (Tn7575) without cas6 gene and ribozyme 
            flanked PaqCI entry site..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9872)
  AUTHORS   Hsieh SC, Peters JE
  TITLE     Discovery and characterization of novel type I-D CRISPR-guided 
            transposons identified among diverse Tn7-like elements in 
            cyanobacteria.
  JOURNAL   Nucleic Acids Res. 2023 Jan 25;51(2):765-782. doi: 
            10.1093/nar/gkac1216.
  PUBMED    36537206
REFERENCE   2  (bases 1 to 9872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2023-01-25- 25"; volume: "51"; issue: 
            "2"; pages: "765-782"
COMMENT     Sequence Label: pOPO794
FEATURES             Location/Qualifiers
     source          1..9872
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(96..974)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1001..1285
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1208..1227
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     terminator      6021..6048
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        6055..6089
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     ncRNA           6101..6137
                     /product="self-cleaving hammerhead ribozyme (Ferre-D'Amare 
                     and Scott, 2010)"
                     /label=hammerhead ribozyme
                     /ncRNA_class="hammerhead_ribozyme"
     ncRNA           6174..6241
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     primer_bind     complement(6318..6335)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      6488..6574
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      6666..6693
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        6712..6803
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6804..6872,6873..7664)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7022..7041)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7706..8161
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7793..7812)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8003..8024
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     rep_origin      8272..8860
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8761..8780
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9014..9031
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9046..9186
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9112..9131)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9272..9294
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(9288..9479)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 atcgatgcat aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac
       61 tccgtcaagc cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca
      121 ttcacttttt cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta
      181 aatacccgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata
      241 ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag
      301 cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag
      361 caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg
      421 tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct
      481 tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc
      541 ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc
      601 gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca
      661 tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga
      721 tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa
      781 acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata
      841 taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc
      901 ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt
      961 tgcgcttcag ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat
     1021 tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta
     1081 accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt
     1141 aacaaaagtg tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca
     1201 ctttgctatg ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta
     1261 tcgcaactct ctactgtttc tccatacccg tttttttggg ctagcaggag gaattcacca
     1321 tgaagccctt actccagact cttctacttc aaactctacc gtctgacatt gatccagtat
     1381 tgctggctta tgttgagact gtattacctg ctgtagaaca ggagttcggt ctgattccag
     1441 tttctaacga tgttagtcaa actgagcaaa gcttgctggt tcacattctc aatagtttgc
     1501 tgactgcctg gaacttgttc ccgttcctat cctcaaaatt ggcgctatca gacaacgaga
     1561 aacgattgct ttgcttgagt cttatctttc aaaatgacaa cagtaagtgc cagagccgag
     1621 agaataattc gcctcctacg cttggtgtta atgaaaccct aaaacagtac cgagcatttg
     1681 gagacaaact aaatctggcg gcgttctgga ctgagtggcg tgagtatttg acagatatag
     1741 tttatttaac ccaaagcacc caaccgcatg ctttgtcaat caagaagact gctctcaaaa
     1801 ttcctgctaa tcgcctagag ttgcctctac gtccattgct tctgtttggg gcgatcgctg
     1861 cccagcttac tgatcccgct gatattgtca tcaaaccgga aggcaatgtc ttaaaagaat
     1921 gcttgaaacg tctgggtgtt cagcatcaac tcacttatca tcgagtacgc gcctgcacag
     1981 gactcttaac tgagggcatc cacaatgcca ttatgcgttt tgtacaacct ctcgactggc
     2041 aatccattct gttctttgcc caagggatcg tttatttggt tccctctgac agcaaactgc
     2101 ttgattgctc aaaactacaa acctttatct gggagcagat tagtgagcag ttagcaatca
     2161 gtatgcttcg aggaaaagtt ggtttcaagc gagatggcaa agggatgaaa gctgcgcctc
     2221 aaacgctgga gctattctca ccagctcaat tcattcggaa tctacccact gtcattaatg
     2281 cctgtgtcaa gaatgagacg gaacctgcaa cacctaaacg actggcaaat cttgaccttg
     2341 atccaacaga tcgagaattt ttagcttgtg gggcggatct aagaagcgat cgcattgcag
     2401 aatttatcct tttcttgcag aagaagtttt ttgggactaa tcctgaattt atcgatcgag
     2461 ttctcgacca actgggcatt caatctgcca tcactccaga acagactcaa atcttctcaa
     2521 gcggggtaaa taggggatgg tatcacgcag ccgcttatta cgtcgccagt aaccctcact
     2581 tagactcaga tgatgtaacg atcaagcttc aggagatcgc caatcaacta gtggactggg
     2641 ctgaagagaa agaacttttt ccacctcaaa tcagtccgac tcgtgaggtt ttcaatcaat
     2701 acttagctca gtacttggaa ttcccaaggt ggaataattc agcgccatcc tttcaacatg
     2761 aattggcaac gtatgtagcg gcaaaaacaa aagccgctaa acaaccaatt tgctccctca
     2821 gttccagtga atttgactca gaagaccagc tagattctgt tgtgctattc aagcctcagc
     2881 agtatagcaa caagaatccc ctcggagggt ggcaactgaa gcgaggaatt tctaaactct
     2941 ggtcattaga gatgcttctg cggcagtctt tctggtcagt ttccgcaggc aagctggaag
     3001 agcgacaacc tgtctttctg tacttttttc ccagtttggt tcattcccct caggtcgcaa
     3061 aagcgattcg tatcctagtc aatcaactta aacgagtcaa tttgtggaaa gtttgtcgat
     3121 tctggcaggt acacgggatg aatgctaata ccctgcgctt ctactcttgg ctgaagtctg
     3181 atcccagacc agacgataat ggtaaacgtg atctcccttt cctagcaatc acttacacca
     3241 ccacagctgg taaaacgacg acacaggctt ggattgaacc tgcttttttg gtattggcgc
     3301 tctcacttgt gctgggcatt aaagtagtcg ctacggcaag tccagtacca ctttataaca
     3361 ggagtagcga gttccccgaa acagtgaaga tagacggacc tgcgagcttc tggagcctgt
     3421 tgggtttgcc aaccagcatt cggctggaag agaagatcca gggacgggtc aaacacctga
     3481 atgattggct cgatcgccta ctaattgctt acagtattca ccttgactgt caagcgaatc
     3541 cccctgactc tcgttggcaa ttgtttcccg gtacagtgca ggctataaca actgatgtct
     3601 cagcgatttt tagccttgct cattcgcatt ttcaaagtct ttcggatgac agagttcaac
     3661 gatactggca ctttgctcaa gtatgggcag caggagattc aaacatggaa cagcaattgg
     3721 agctaactca gcgactggtc caggaatatc gcgcgttcta ccaagttcat gtagcgaaag
     3781 caacccacgc gatcgtgctc ccgctgctaa aggttttaga ggagattctt tcaactccta
     3841 gtgatctgcc aattgaaact ctgatattac agacatcggg acggttacat gacgctttga
     3901 agcgacagga accctacaaa cgccccctga ttatggatca gtctttatca attgagattc
     3961 gtgtcaataa cgagattcag gcaattcaac gatttgtcac cacttgcatc aatgatttat
     4021 ttttagctca gtacaaaggc gatcgcgccc tactgcaaga acaccgtaac cgaattaaat
     4081 cgggtgcaga gtttgcgtat cgtttactag ctatgcagga gccaaaacca aaaacgaaaa
     4141 agcaagaaat tgaaggagca cagatatgag tgtgttagaa gaattgaaac ctcaattcca
     4201 ttcggctttt ccccgtctgg catccgggaa gtatgttcat gtcttcctgg tacgtcacag
     4261 tcaatccttt cccgtgtttc aaacagatgg tgtcctcaat acggctcgaa ctcaagctgg
     4321 cttggattcc aaagatgata aatctaggca atcgatcaac cgattggtca tgttcaagcg
     4381 caagcaagta acacccgaac gactggttgg acgacaactg ctacgatcgc taggactaac
     4441 cagtgctgta gaaggcgaag tcaacttttg cgaatacaac agtcagaaat cgtgcaagaa
     4501 atgcccagac tgtattatct acggattcgg ggttggagac aaaggtgcag aacgctcgaa
     4561 agtctactca gactcagcgt tctcgctcag tccttatgaa acttcccacc gcagtttcac
     4621 cttcaatgcg ccgtcagaaa atggcacgat gagtgaaaag ggagaactgc gaagttcgat
     4681 caacgatcta gaccatgtcc tgccggaagt gatgtttcct agtgttgaaa cgctgcgtga
     4741 tcccacattt gaggggttta tttatgtgct gggcaacctg attcgcacgc gccgctatgg
     4801 aggtcaagaa tcccgaaccg ggacgatgat gaatcacatt gttggtattg ccttttgcga
     4861 tggtgaagtt ttcagcaact tgtatttcac acaagcgcta tatgatgcct tgaatgcaaa
     4921 gggggatatt tgcgtgacga tcgaggactt tcgacatgag gcagcaaggg tggcaaaaaa
     4981 ctgtctgaaa caggagccag ttcgcagcat cacaattttt ggggctgaac tcgatgcctt
     5041 gctcgaagaa gtgacgggca tctatcagga tgaagttaaa cagcgcctta ttatgaccac
     5101 gctttaccaa cagacccaga tctacagtga aagctacgga gcaatttttc ctgagaagaa
     5161 gaaagaggaa aagaaaaaag cacagcaagg ctcttcgatc tgagggatgg tggaataatg
     5221 gttttctatt tttgccattt aaccttgcat gacaatgttt tctttgcctc acgggagatg
     5281 ggaactctct atgagacaga aaaatacctg cacaattggg cacttagcta tgcgctattt
     5341 gaaagcgcct acattcctag accttatcga ttggaagggg cagtcgccca gcgtcctacg
     5401 tatctagaga ttgatagtga gcaaaacctg ttacatctga atcagcaagg aatttatgtt
     5461 tttcccgccc tgccgcttca gtggacatat caggtcaata cattcaatgt gtctcaagcc
     5521 ttctaccatc aaaaatcaag acggttgggc tcgggaggac atattctcaa ttacccaatc
     5581 agctatggac gggcaaagga actcgcagtt ggcagcaagt acagaacctt catcattgct
     5641 cctgccaaaa cgagaatccc ccgctggata agactaggca aatggtcagc gaaaattcga
     5701 gtcgagatca tccccattcc taaaagtgga attcaaaggg cagcaggcga gtatgtttct
     5761 gagcatcccc tcaatcctgt agatctctct aaaacgaccc gattattgct gtataaccgg
     5821 attgtcatgc caccttctag tctggtgagc caggcaaaat tgttgggtga ctattgggag
     5881 atctcaaaac cagctcagtg ggctaaattg tctgagtggt caagcttaga gaatttgcct
     5941 gaaacgattt gtttgccaag aggagttgct tatggagcta acactctcgt taccacacct
     6001 taactaaaaa aaatcaaact ggctcacctt cgggtgggcc tttttgcgtt tatattgaca
     6061 gctagctcag tcctaggtat aatgctagct acttttcagt ctgatgagtc cgtgaggacg
     6121 aaacgagtaa gctcgtcact gaaaggtaag caggtgtaag gccacctgcg taaggccggc
     6181 atggtcccag cctcctcgct ggcgccggct gggcaacatg cttcggcatg gcgaatggga
     6241 cgtacccggg gatcctctag agtcgacctg caggcatgca agcttggctg ttttggcgga
     6301 tgagagaaga ttttcagcct gatacagatt aaatcagaac gcagaagcgg tctgataaaa
     6361 cagaatttgc ctggcggcag tagcgcggtg gtcccacctg accccatgcc gaactcagaa
     6421 gtgaaacgcc gtagcgccga tggtagtgtg gggtctcccc atgcgagagt agggaactgc
     6481 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     6541 tttgtcggtg aacgctctcc tgagtaggac aaatccgccg ggagcggatt tgaacgttgc
     6601 gaagcaacgg cccggagggt ggcgggcagg acgcccgcca taaactgcca ggcatcaaat
     6661 taagcagaag gccatcctga cggatggcct ttttgcgttt ctacaaactc ttttgtttat
     6721 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     6781 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     6841 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     6901 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     6961 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     7021 tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca
     7081 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     7141 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     7201 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     7261 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     7321 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     7381 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     7441 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     7501 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     7561 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     7621 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     7681 actcatatat actttagatt gatttacgcg ccctgtagcg gcgcattaag cgcggcgggt
     7741 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     7801 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     7861 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     7921 ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     7981 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     8041 atctcgggct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     8101 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt
     8161 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     8221 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     8281 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     8341 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     8401 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     8461 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     8521 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     8581 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     8641 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     8701 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     8761 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     8821 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     8881 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     8941 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     9001 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt
     9061 tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg
     9121 ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg
     9181 gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
     9241 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
     9301 ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc
     9361 gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt
     9421 aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact
     9481 gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag
     9541 aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag
     9601 ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc
     9661 cagctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga
     9721 ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga
     9781 ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta
     9841 actgtgataa actaccgcat taaagctagc tt
//
