LOCUS       sequence-383149-        8055 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of full length Human epidermal growth factor 
            (Her)-2-Fc fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8055)
  TITLE     McLellan lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8055)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pcDNA3.1(-) Full length Her2(ERBB2)-Fc
FEATURES             Location/Qualifiers
     source          1..8055
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        145..524
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        525..728
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     679..699
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     773..792
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        773..791
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      880..889
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(3556..3573)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3562..3786
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3832..4260
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3919..3938)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4129..4150
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4269..4289)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4274..4603
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4454..4589
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4516..4535
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4670..5464
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4724..4743)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5334..5353
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5638..5759
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5675..5694)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5729..5748
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5808..5824)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5808..5824)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5821..5843)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5832..5848
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5856..5862,5863..5880,5881..5886))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5901..5922
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6039..6056)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6210..6798)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6290..6309)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6969..7760,7761..7829))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7592..7611
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7830..7934)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8009..8028)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag
       61 gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg
      121 atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa tagtaatcaa
      181 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
      241 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
      301 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
      361 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
      421 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
      481 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
      541 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
      601 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
      661 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
      721 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
      781 ctcactatag ggagacccaa gctggctagc gtttaaacgg gccctctaga ctcgagcggc
      841 cgccactgtg ctggatatct gcagaattcg gcctctgagg ccaccatgga actggctgca
      901 ctgtgtcgct ggggtctcct tctcgccttg ctgcctcctg gtgctgcgtc cacgcaagtc
      961 tgtactggaa ccgacatgaa gttgagactc cccgcgtctc ctgagacaca cctcgacatg
     1021 ttgcggcatc tctatcaggg gtgtcaagta gtgcaaggga atcttgaact gacttatctg
     1081 ccgaccaacg catcactcag ttttcttcaa gatatacaag aggttcaggg ctatgtcctg
     1141 atcgcgcata accaggtgag acaggtccct ctgcagcgac tgcggattgt gcgaggtaca
     1201 cagttgtttg aagacaacta cgcactcgca gttctcgaca acggtgaccc actgaacaat
     1261 accactcctg tcactggagc ctcaccagga ggactgagag aacttcagct tcgctccctg
     1321 acggaaatcc ttaaaggtgg tgttctgatt caacgaaacc ctcagctttg ctaccaggat
     1381 accattcttt ggaaggacat ctttcataaa aacaatcaac tcgcactcac attgattgat
     1441 acaaatcgca gcagggcttg tcatccttgt tcccctatgt gtaaagggtc aagatgctgg
     1501 ggtgagagtt ctgaggattg ccagagtctt acacgcacgg tctgcgcagg tggttgtgcc
     1561 cggtgtaagg ggcctttgcc taccgattgc tgtcacgagc aatgcgccgc aggctgtaca
     1621 ggcccaaaac actctgattg ccttgcatgc ttgcacttta accactccgg aatctgcgaa
     1681 ctccattgcc cagctctcgt tacctacaac accgatacgt tcgaaagtat gccaaaccca
     1741 gaggggcgct acacatttgg ggcatcatgt gtaaccgcgt gcccgtacaa ctacctgtcc
     1801 accgacgtcg gaagctgcac cctcgtttgc ccattgcaca accaggaagt gaccgctgaa
     1861 gatggtacac aaaggtgtga aaaatgctcc aaaccatgcg cgcgcgtatg ctacggcctg
     1921 gggatggaac atctgaggga ggtcagagcg gtcacctcag caaatatcca agagttcgct
     1981 ggatgtaaaa agatattcgg ctccttggct tttcttcccg agagcttcga cggtgacccc
     2041 gcgagcaaca cagccccact tcaacctgaa cagctccaag tttttgaaac tttggaagag
     2101 atcacgggtt atctgtacat atctgcttgg cccgactccc tgccagacct gtccgtcttc
     2161 cagaaccttc aggtcattcg aggacggatc cttcacaacg gagcgtactc tttgacattg
     2221 caaggattgg gcattagttg gcttggcctc cgaagcctcc gcgaacttgg ttcaggattg
     2281 gcactcatac accataatac ccatctctgt tttgtccaca ctgttccgtg ggatcagctc
     2341 ttcagaaacc cccaccaagc cctcttgcac accgcaaacc gacccgagga cgagtgcgtg
     2401 ggtgagggac tcgcatgcca ccaactgtgt gcccgaggcc actgctgggg cccaggtccg
     2461 acacaatgcg tcaactgcag ccagtttctc cggggacagg agtgcgtcga agaatgtcga
     2521 gttttgcagg ggcttcctcg ggagtatgtt aacgcgcggc attgtctgcc gtgccaccct
     2581 gagtgtcagc cgcaaaatgg gtcagttacg tgtttcggcc cggaggcgga tcaatgcgtg
     2641 gcatgcgctc attacaaaga tccgccgttc tgcgttgcga ggtgtccatc tggagtgaaa
     2701 ccggatctca gttacatgcc aatttggaaa ttccccgatg aagaaggagc ttgccaaccg
     2761 tgtccaataa actgcaccca cagttgcgtt gatctcgatg acaagggttg tccagcggag
     2821 caacgggcat ccccgctcac gggtaccgac aaaactcaca catgcccacc gtgcccagca
     2881 cctgaactcc tggggggacc gtcagtcttc ctcttccccc caaaacccaa ggacaccctc
     2941 atgatctccc ggacccctga ggtcacatgc gtggtggtgg acgtgagcca cgaagaccct
     3001 gaggtcaagt tcaactggta cgtggacggc gtggaggtgc ataatgccaa gacaaagccg
     3061 cgggaggagc agtacaacag cacgtaccgt gtggtcagcg tcctcaccgt cctgcaccag
     3121 gactggctga atggcaagga gtacaagtgc aaggtctcca acaaagccct cccagccccc
     3181 atcgagaaaa ccatctccaa agccaaaggg cagccccgag aaccacaggt gtacaccctg
     3241 cccccatccc gggaggagat gaccaagaac caggtcagcc tgacctgcct ggtcaaaggc
     3301 ttctatccca gcgacatcgc cgtggagtgg gagagcaatg ggcagccgga gaacaactac
     3361 aagaccacgc ctcccgtgct ggactccgac ggctccttct tcctctacag caagctcacc
     3421 gtggacaaga gcaggtggca gcaggggaac gtcttctcat gctccgtgat gcacgaggct
     3481 ctgcacaacc actacacgca gaagagcctc tccctgtctc cgggtaaata agcttaagtt
     3541 taaaccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     3601 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     3661 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     3721 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
     3781 ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct
     3841 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     3901 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     3961 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     4021 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     4081 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     4141 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     4201 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     4261 aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     4321 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     4381 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     4441 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     4501 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca
     4561 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg
     4621 tatatccatt ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca
     4681 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
     4741 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
     4801 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc
     4861 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
     4921 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
     4981 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
     5041 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
     5101 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
     5161 gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct
     5221 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
     5281 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
     5341 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
     5401 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
     5461 ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga
     5521 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
     5581 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac
     5641 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     5701 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     5761 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
     5821 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     5881 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     5941 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     6001 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     6061 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     6121 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     6181 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     6241 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     6301 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     6361 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     6421 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     6481 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     6541 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     6601 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     6661 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     6721 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     6781 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     6841 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     6901 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     6961 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     7021 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     7081 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     7141 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     7201 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     7261 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     7321 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     7381 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     7441 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     7501 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     7561 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     7621 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     7681 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     7741 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     7801 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     7861 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     7921 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat
     7981 ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     8041 agtatctgct ccctg
//
