LOCUS       sequence-383152-        8021 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tetracycline inducible expression of Luciferase enzyme in mammalian 
            cells with inclusion of rtTA G72V mutation, splice site and high 
            sensitivity mutations for increased promotor tightness.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8021)
  AUTHORS   Saunderson SC, Hosseini-Rad SMA, McLellan AD
  TITLE     Noise-Reduction and Sensitivity-Enhancement of a Sleeping 
            Beauty-Based Tet-On System.
  JOURNAL   Genes (Basel). 2022 Sep 20;13(10). pii: genes13101679. doi: 
            10.3390/genes13101679.
  PUBMED    36292564
REFERENCE   2  (bases 1 to 8021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            (Basel)."; date: "2022-09-20"; volume: "13(10). pii: genes13101679. 
            doi"; pages: " 10.3390/genes13101679"
COMMENT     Sequence Label: pSBtet rtTA G72V SE Delta-splice GP Luc
FEATURES             Location/Qualifiers
     source          1..8021
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(289..291,292..294,295..1005)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MLSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(289..291,292..294,295..1005)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MLSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(334..355)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(595..614)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     942..963
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1015..1071
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(1072..1689,1690..1695,1696..1731,1732..1734,1735..1770,
                     1771..1773,1774..1809,1810..1815)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKIINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEVESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             1822..1878
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             1879..2478
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1879..1898)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2375..2395
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(2533..2550)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2539..2763
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(3089..3106)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3260..3848)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3340..3359)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4019..4810,4811..4879))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4642..4661
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4880..4984)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5387..5699
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    5395..5413
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5431..5449
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5466..5484
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5502..5520
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5538..5556
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5573..5591
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5609..5627
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     5672..5696
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      5772..5781
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5778..7430
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    7466..7587
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7477..7496)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7531..7550
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 tctctgcgca cgcgcagaac gcttcgacca atgaaaacac aggaagccgt ccgcgcaacc
       61 gcgttgcgtc acttctgccg cccctgtttc aaggtatata gccgtagacg gaacttcgcc
      121 tttctctcgg ccttagcgcc atttttttgg gtgagtgttt tttggttcct gcgttgggat
      181 tccgtgtaca atccatagac atctgacctc ggcacttagc atcatcacag caaactaact
      241 gtagcctttc tctctttccc tgtagaaacc tctgcacctg aggccaccat gttgagcaag
      301 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
      361 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
      421 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
      481 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
      541 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
      601 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
      661 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
      721 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
      781 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
      841 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
      901 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
      961 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagggcag tggagctact
     1021 aacttcagcc tgctgaagca ggctggtgac gtcgaggaga atcctggccc catgtctaga
     1081 ctggacaaga gcaaaatcat aaacggcgct ctggaattac tcaatggagt cggtatcgaa
     1141 ggcctgacga caaggaaact cgctcaaaag ctgggagttg agcagcctac cctgtactgg
     1201 cacgtgaaga acaagcgggc cctgctcgat gccctgccaa tcgagatgct ggacaggcat
     1261 catacccact cctgccccct ggaagtggag tcatggcaag actttctgcg gaacaacgcc
     1321 aagtcatacc gctgtgctct cctctcacat cgcgacgggg ctaaagtgca tctcggcacc
     1381 cgcccaaccg agaaacaata cgaaaccctg gaaaatcagc tcgcgttcct gtgtcaacaa
     1441 ggcttctccc tggagaacgc actttacgct ctgtccgccg ttggccactt tacactgggc
     1501 tgcgtattgg aggaacagga gcatcaagtt gcaaaagagg aaagagagac acctaccacc
     1561 gattctatgc ccccacttct gaaacaagca attgagctgt tcgaccggca aggagccgaa
     1621 cctgccttcc ttttcggcct ggaactaatc atatgtggcc tggagaaaca gctaaagtgc
     1681 gaaagcggcg ggccggccga cgcccttgac gattttgact tagacatgct cccagccgat
     1741 gcccttgacg actttgacct tgatatgctg cctgctgacg ctcttgacga ttttgacctt
     1801 gacatgctcc ccgggtccgg cgctactaac ttcagcctgc tgaagcaggc tggtgacgtc
     1861 gaggagaatc ctggtcccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     1921 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     1981 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     2041 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     2101 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     2161 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     2221 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
     2281 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     2341 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
     2401 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     2461 cgcaagcccg gtgcctgatt cgaaggcctg tcgtgaagct tggggatcaa ttctctagag
     2521 ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
     2581 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
     2641 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
     2701 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta
     2761 tggcttctga ggcggaaaga accagctggg cctactagct actcgggacc ccttaccgaa
     2821 acatcgccgc attctgcaga ggagtcgagt gtatgtaaac ttctgaccca ctgggaatgt
     2881 gatgaaagaa ataaaagctg aaatgaatca ttctctctac tattattctg atatttcaca
     2941 ttcttaaaat aaagtggtga tcctaactga cctaagacag ggaattttta ctaggattaa
     3001 atgtcaggaa ttgtgaaaaa gtgagtttaa atgtatttgg ctaaggtgta tgtaaacttc
     3061 cgacttcaac tgtataggga tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     3121 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     3181 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     3241 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     3301 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     3361 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     3421 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     3481 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     3541 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     3601 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     3661 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     3721 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     3781 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     3841 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     3901 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     3961 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     4021 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     4081 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     4141 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     4201 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     4261 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     4321 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     4381 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     4441 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     4501 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     4561 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     4621 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     4681 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     4741 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     4801 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     4861 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     4921 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     4981 cgcgcacatt tccccgaaaa gtgccacctg atgcggtgtg aaataccgca cagatgcgta
     5041 aggagaaaat accgcatcag gaaattgtaa gcgttaatat tttgttaaaa ttggatccct
     5101 atacagttga agtcggaagt ttacatacac ttaagttgga gtcattaaaa ctcgtttttc
     5161 aactactcca caaatttctt gttaacaaac aatagttttg gcaagtcagt taggacatct
     5221 actttgtgca tgacacaagt catttttcca acaattgttt acagacagat tatttcactt
     5281 ataattcact gtatcacaat tccagtgggt cagaagttta catacactaa gttcgactcc
     5341 tctgcagaat gcggcgatgt ttcggtaagg ggtccgctat ctagacgagt ttactcccta
     5401 tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa cgatgtcgag
     5461 tttactccct atcagtgata gagaacgtat gtcgagttta ctccctatca gtgatagaga
     5521 acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgagtt tatccctatc
     5581 agtgatagag aacgtatgtc gagtttactc cctatcagtg atagagaacg tatgtcgatt
     5641 aggcgtgtac ggtgggcgcc tataaaagca gagctcgttt agtgaaccgt cagatcgcct
     5701 ggagccaatt ccaactcttt tgccttatac ctactttccg taccacttcc taccctcgaa
     5761 aggcctctga ggccaccatg gaagatgcca aaaacattaa gaagggccca gcgccattct
     5821 acccactcga agacgggacc gccggcgagc agctgcacaa agccatgaag cgctacgccc
     5881 tggtgcccgg caccatcgcc tttaccgacg cacatatcga ggtggacatt acctacgccg
     5941 agtacttcga gatgagcgtt cggctggcag aagctatgaa gcgctatggg ctgaatacaa
     6001 accatcggat cgtggtgtgc agcgagaata gcttgcagtt cttcatgccc gtgttgggtg
     6061 ccctgttcat cggtgtggct gtggccccag ctaacgacat ctacaacgag cgcgagctgc
     6121 tgaacagcat gggcatcagc cagcccaccg tcgtattcgt gagcaagaaa gggctgcaaa
     6181 agatcctcaa cgtgcaaaag aagctaccga tcatacaaaa gatcatcatc atggatagca
     6241 agaccgacta ccagggcttc caaagcatgt acaccttcgt gacttcccat ttgccacccg
     6301 gcttcaacga gtacgacttc gtgcccgaga gcttcgaccg ggacaaaacc atcgccctga
     6361 tcatgaacag tagtggcagt accggattgc ccaagggcgt agccctaccg caccgcaccg
     6421 cttgtgtccg attcagtcat gcccgcgacc ccatcttcgg caaccagatc atccccgaca
     6481 ccgctatcct cagcgtggtg ccatttcacc acggcttcgg catgttcacc acgctgggct
     6541 acttgatctg cggctttcgg gtcgtgctca tgtaccgctt cgaggaggag ctattcttgc
     6601 gcagcttgca agactataag attcaatctg ccctgctggt gcccacacta tttagcttct
     6661 tcgctaagag cactctcatc gacaagtacg acctaagcaa cttgcacgag atcgccagcg
     6721 gcggggcgcc gctcagcaag gaggtaggtg aggccgtggc caaacgcttc cacctaccag
     6781 gcatccgcca gggctacggc ctgacagaaa caaccagcgc cattctgatc acccccgaag
     6841 gggacgacaa gcctggcgca gtaggcaagg tggtgccctt cttcgaggct aaggtggtgg
     6901 acttggacac cggtaagaca ctgggtgtga accagcgcgg cgagctgtgc gtccgtggcc
     6961 ccatgatcat gagcggctac gttaacaacc ccgaggctac aaacgctctc atcgacaagg
     7021 acggctggct gcacagcggc gacatcgcct actgggacga ggacgagcac ttcttcatcg
     7081 tggaccggct gaagagcctg atcaaataca agggctacca ggtagcccca gccgaactgg
     7141 agagcatcct gctgcaacac cccaacatct tcgacgccgg ggtcgccggc ctgcccgacg
     7201 acgatgccgg cgagctgccc gccgcagtcg tcgtgctgga acacggtaaa accatgaccg
     7261 agaaggagat cgtggactat gtggccagcc aggttacaac cgccaagaag ctgcgcggtg
     7321 gtgttgtgtt cgtggacgag gtgcctaaag gactgaccgg caagttggac gcccgcaaga
     7381 tccgcgagat tctcattaag gccaagaagg gcggcaagat cgccgtgtaa ggcctgtcag
     7441 gccaagcttc catcgataga catgataaga tacattgatg agtttggaca aaccacaaca
     7501 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
     7561 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag
     7621 gttcaggggg agatgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtacc
     7681 taggcgagac cctgtctcac aaaataaagt aagcccggac tgagtgcgga aaggcgggcc
     7741 tggcgggtct ggtctcccca tgcgggccac cagaggccct gcagccttca gtcgcttgaa
     7801 ggggtaatgg cgcttccact cacaaacatg gcggacagag cgtgtgaacg agatgaacag
     7861 cccctcaaaa atatggccgc cgaggctgga cggccgtgcc ccagcagcac cgcctccgcg
     7921 ccccacgtga tctctcgccg ggcacagcgc tgaccgcgga ggtccaaccg gaagaatgtc
     7981 cggattggac attcggaaga gggcccgcct tccctgggga a
//
