LOCUS       sequence-383157-        8137 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirial plasmid for nuclear iRFP-720 expression with blasticidin
            resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8137)
  AUTHORS   Gabriel CH, Del Olmo M, Rizki Widini A, Roshanbin R, Woyde J, Hamza 
            E, Gutu NN, Zehtabian A, Ewers H, Granada A, Herzel H, Kramer A
  TITLE     Circadian period is compensated for repressor protein turnover rates
            in single cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2024 Aug 20;121(34):e2404738121. doi: 
            10.1073/pnas.2404738121. Epub 2024 Aug 14.
  PUBMED    39141353
REFERENCE   2  (bases 1 to 8137)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2024-08-20"; volume: "121(34):e2404738121. 
            doi"; pages: " 10.1073/pnas.2404738121. Epub 2024 Aug 14"
COMMENT     Sequence Label: pL6-H2B-iRFP720-bla
FEATURES             Location/Qualifiers
     source          1..8137
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(7..12,13..30,31..37)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    45..61
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     50..72
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     69..85
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     69..85
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     104..124
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        106..124
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        152..378
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             379..559
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    606..731
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1224..1457
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1642..1686
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1969..2479
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2398..2416
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2491..2868
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     CDS             2887..3834
                     /codon_start=1
                     /product="near-infrared fluorescent protein with an 
                     emission peak at 720 nm, engineered from a bacterial 
                     phytochrome (Shcherbakova and Verkhusha, 2013)"
                     /label=iRFP720
                     /note="derived from Rhodopseudomonas palustris RpBphP2"
                     /translation="AEGSVARQPDLLTCDDEPIHIPGAIQPHGLLLALAADMTIVAGSD
                     NLPELTGLAIGALIGRSAADVFDSETHNRLTIALAEPGAAVGAPITVGFTMRKDAGFIG
                     SWHRHDQLIFLELEPPQRDVAEPQAFFRRTNSAIRRLQAAETLESACAAAAQEVRKITG
                     FDRVMIYRFASDFSGSVIAEDRCAEVESKLGLHYPASFIPAQARRLYTINPVRIIPDIN
                     YRPVPVTPDLNPVTGRPIDLSFAILRSVSPNHLEFMRNIGMHGTMSISILRGERLWGLI
                     VCHHRTPYYVDLDGRQACELVAQVLAWQIGVMEE"
     primer_bind     complement(3874..3894)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3879..4208
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4059..4194
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        4256..4303
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4322..4720
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             4807..5040
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5112..5233
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5149..5168)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5203..5222
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5273..5408
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5428..5447)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5429..5447)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5457..5474)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5457..5473)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5466..5488)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5615..6070
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5702..5721)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5912..5933
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6096..6200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6201..6269,6270..7061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6419..6438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7232..7820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7721..7740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7974..7991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8108..8129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
       61 atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta
      121 aagggaacaa aagctggagc tgcaagctta atgtagtctt atgcaatact cttgtagtct
      181 tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa gcaccgtgca
      241 tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca acagacgggt
      301 ctgacatgga ttggacgaac cactgaattg ccgcattgca gagatattgt atttaagtgc
      361 ctagctcgat acataaacgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
      421 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
      481 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
      541 gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag
      601 aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc
      661 ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt
      721 gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa
      781 ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag
      841 aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg
      901 gacagctaca accatccctt cagacaggat cagaagaact tagatcatta tataatacag
      961 tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag
     1021 acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc
     1081 ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa
     1141 gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag
     1201 agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga
     1261 agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt
     1321 atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa
     1381 ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta
     1441 aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct
     1501 gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc
     1561 tggatggagt gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa
     1621 gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca
     1681 agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg
     1741 atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga
     1801 gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc
     1861 gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag atccattcga
     1921 ttagtgaacg gatctcgacg gtatcgatca ctttggccgc ggctcgaggg ggttggggtt
     1981 gcgccttttc caaggcagcc ctgggtttgc gcagggacgc ggctgctctg ggcgtggttc
     2041 cgggaaacgc agcggcgccg accctgggtc tcgcacattc ttcacgtccg ttcgcagcgt
     2101 cacccggatc ttcgccgcta cccttgtggg ccccccggcg acgcttcctg ctccgcccct
     2161 aagtcgggaa ggttccttgc ggttcgcggc gtgccggacg tgacaaacgg aagccgcacg
     2221 tctcactagt accctcgcag acggacagcg ccagggagca atggcagcgc gccgaccgcg
     2281 atgggctgtg gccaatagcg gctgctcagc agggcgcgcc gagagcagcg gccgggaagg
     2341 ggcggtgcgg gaggcggggt gtggggcggt agtgtgggcc ctgttcctgc ccgcgcggtg
     2401 ttccgcattc tgcaagcctc cggagcgcac gtcggcagtc ggctccctcg ttgaccgaat
     2461 caccgacctc tctccccagg gggatctacc atgccagagc cagcgaagtc tgctcccgcc
     2521 ccgaaaaagg gctccaagaa ggcggtgact aaggcgcaga agaaaggcgg caagaagcgc
     2581 aagcgcagcc gcaaggagag ctattccatc tatgtgtaca aggttctgaa gcaggtccac
     2641 cctgacaccg gcatttcgtc caaggccatg ggcatcatga actcgtttgt gaacgacatt
     2701 ttcgagcgca tcgcaggtga ggcttcccgc ctggcgcatt acaacaagcg ctcgaccatc
     2761 acctccaggg agatccagac ggccgtgcgc ctgctgctgc ctggggagtt ggccaagcac
     2821 gccgtgtccg agggtactaa ggccatcacc aagtacacca gcgctaagga tccaccggtc
     2881 gccaccgcgg aaggatccgt cgccaggcag cctgacctct tgacctgcga cgatgagccg
     2941 atccatatcc ccggtgccat ccaaccgcat ggactgctgc tcgccctcgc cgccgacatg
     3001 acgatcgttg ccggcagcga caaccttccc gaactcaccg gactggcgat cggcgccctg
     3061 atcggccgct ctgcggccga tgtcttcgac tcggagacgc acaaccgtct gacgatcgcc
     3121 ttggccgagc ccggggcggc cgtcggagca ccgatcactg tcggcttcac gatgcgaaag
     3181 gacgcaggct tcatcggctc ctggcatcgc catgatcagc tcatcttcct cgagctcgag
     3241 cctccccagc gggacgtcgc cgagccgcag gcgttcttcc gccgcaccaa cagcgccatc
     3301 cgccgcctgc aggccgccga aaccttggaa agcgcctgcg ccgccgcggc gcaagaggtg
     3361 cggaagatta ccggcttcga tcgggtgatg atctatcgct tcgcctccga cttcagcggg
     3421 tccgtgatcg cagaggatcg gtgcgccgag gtcgagtcaa aactaggcct gcactatcct
     3481 gcctcattca tcccggcgca ggcccgtcgg ctctatacca tcaacccggt acggatcatt
     3541 cccgatatca attatcggcc ggtgccggtc accccagacc tcaatccggt caccgggcgg
     3601 ccgattgatc ttagcttcgc catcctgcgc agcgtctcgc ccaaccatct ggagttcatg
     3661 cgcaacatag gcatgcacgg cacgatgtcg atctcgattt tgcgcggcga gcgactgtgg
     3721 ggattgatcg tttgccatca ccgaacgccg tactacgtcg atctcgatgg ccgccaagcc
     3781 tgcgagctag tcgcccaggt tctggcctgg cagatcggcg tgatggaaga gtaaacgcgt
     3841 accggttagt aatgagtttg gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa
     3901 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     3961 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     4021 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     4081 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     4141 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     4201 tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcag cacgtgttga
     4261 caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga ggaactaaac
     4321 catggccaag cctttgtctc aagaagaatc caccctcatt gaaagagcaa cggctacaat
     4381 caacagcatc cccatctctg aagactacag cgtcgccagc gcagctctct ctagcgacgg
     4441 ccgcatcttc actggtgtca atgtatatca ttttactggg ggaccttgtg cagaactcgt
     4501 ggtgctgggc actgctgctg ctgcggcagc tggcaacctg acttgtatcg tcgcgatcgg
     4561 aaatgagaac aggggcatct tgagcccctg cggacggtgc cgacaggtgc ttctcgatct
     4621 gcatcctggg atcaaagcca tagtgaagga cagtgatgga cagccgacgg cagttgggat
     4681 tcgtgaattg ctgccctctg gttatgtgtg ggagggctaa gcacaattcg agctcggtac
     4741 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     4801 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
     4861 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     4921 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     4981 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     5041 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
     5101 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     5161 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     5221 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc
     5281 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     5341 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     5401 tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
     5461 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     5521 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     5581 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc
     5641 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     5701 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     5761 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     5821 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     5881 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     5941 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     6001 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     6061 cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     6121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     6181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     6241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     6301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     6361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     6421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     6481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     6541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     6601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     6661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     6721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     6781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     6841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     6901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     6961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     7021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     7081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     7141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     7201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     7261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     7321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     7381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     7441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     7501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     7561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     7621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     7681 gcagggtcgg aagaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     7741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     7801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     7861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     7921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     7981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     8041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     8101 acgcaattaa tgtgagttag ctcactcatt aggcacc
//
