LOCUS       sequence-383158-        9962 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector to integrate firefly Luciferase C-terminal into the 
            human PER1 Locus to express it as a fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9962)
  AUTHORS   Gabriel CH, Del Olmo M, Rizki Widini A, Roshanbin R, Woyde J, Hamza 
            E, Gutu NN, Zehtabian A, Ewers H, Granada A, Herzel H, Kramer A
  TITLE     Circadian period is compensated for repressor protein turnover rates
            in single cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2024 Aug 20;121(34):e2404738121. doi: 
            10.1073/pnas.2404738121. Epub 2024 Aug 14.
  PUBMED    39141353
REFERENCE   2  (bases 1 to 9962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2024-08-20"; volume: "121(34):e2404738121. 
            doi"; pages: " 10.1073/pnas.2404738121. Epub 2024 Aug 14"
COMMENT     Sequence Label: pDB2-hPER1-Luciferase-CD4-bla
FEATURES             Location/Qualifiers
     source          1..9962
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(59..76)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(230..818)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(310..329)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(989..1780,1781..1849))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1612..1631
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1850..1954)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2022..2040
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     2200..2219
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             3229..4875
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="EDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAH
                     IEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPA
                     NDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSM
                     YTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHAR
                     DPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKI
                     QSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYG
                     LTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSG
                     YVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILL
                     QHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVF
                     VDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(3292..3310)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     CDS             4882..4947
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(4957..4990)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(5073..5093)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5078..5408
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5259..5394
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      5458..5467
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5464..5466,5467..5469,5470..6180)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5509..5530)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5770..5789)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6117..6138
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             6196..6252
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6259..6654
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="AKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    6666..6787
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6703..6722)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6757..6776
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    6815..6848
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     protein_bind    complement(6849..6882)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        7903..8106
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8057..8077
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     8103..8127
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(8201..8275,8276..9472)
                     /codon_start=1
                     /product="truncated human CD4 cell surface glycoprotein 
                     lacking the cytoplasmic tail"
                     /label=CD4 (truncated)
                     /translation="MNRGVPFRHLLLVLQLALLPAATQGKKVVLGKKGDTVELTCTASQ
                     KKSIQFHWKNSNQIKILGNQGSFLTKGPSKLNDRADSRRSLWDQGNFPLIIKNLKIEDS
                     DTYICEVEDQKEEVQLLVFGLTANSDTHLLQGQSLTLTLESPPGSSPSVQCRSPRGKNI
                     QGGKTLSVSQLELQDSGTWTCTVLQNQKKVEFKIDIVVLAFQKASSIVYKKEGEQVEFS
                     FPLAFTVEKLTGSGELWWQAERASSSKSWITFDLKNKEVSVKRVTQDPKLQMGKKLPLH
                     LTLPQALPQYAGSGNLTLALEAKTGKLHQEVNLVVMRATQLQKNLTCEVWGPTSPKLML
                     SLKLENKEAKVSKREKAVWVLNPEAGMWQCLLSDSGQVLLESNIKVLPTWSTPVQPMAL
                     IVLGGVAGLLLFIGLGIFFCVRCRH"
     primer_bind     complement(9790..9806)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9790..9806)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9803..9825)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9814..9830
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9838..9844,9845..9862,9863..9868))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9883..9904
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
       61 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
      121 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
      181 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
      241 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
      301 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
      361 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
      421 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
      481 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
      541 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
      601 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
      661 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
      721 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
      781 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
      841 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
      901 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
      961 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     1021 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     1081 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     1141 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     1201 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     1261 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     1321 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     1381 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     1441 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     1501 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     1561 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     1621 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     1681 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     1741 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     1801 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     1861 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     1921 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     1981 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     2041 cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
     2101 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
     2161 cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac
     2221 tgagagtgca ccatatgggc cggcccttaa ggtttaaact ctgaacccct tcttgggcag
     2281 ggacatgacc tctgtgctga agcaggatcg ggagcggctc cgagccatgc agaagcagca
     2341 gcctcggttt tctgaggacc agcggcggga actgggtgct gtgcactcct gggtccggaa
     2401 gggccaactg cctcgggctc ttgatgtgat ggtgagagaa gcctgggacg gggagaaaaa
     2461 agaattgagc tcaagttcaa gggggagaaa aaagaattga gctcaagttc aagggggaga
     2521 aaaaagaatt gagctcaagt tcaagggatc gaggccaaga gctgatctcc ttgatgtcct
     2581 tggatcatta attctgaaga atgttgattc cactaaattt gctgtggatt atagaatatt
     2641 aagccgcgtg agtctttgca gaacttttca cagcctatcc tatgctaata tgcattgtga
     2701 ctgtcctgta acggcatctg ggtagagggc acaaggcact gtccaacctt gttggaccgc
     2761 aggtgcatct gtgtggactg gtgcttcttg ggagtacatt tcgggaagca cagtgggctg
     2821 ggggtgggaa gctgcgctgg caggttagca gtgagaaccc tgtctgactc tctcatgtcc
     2881 atttctctca ccaaggcctg tgtggactgt gggagcagca cccaagatcc tggtcaccct
     2941 gatgacccac tcttctcaga gctggatgga ctggggctgg agcccatgga agagggtgga
     3001 ggcgagcagg gcagcagcgg tggcggcagt ggtgagggag agggctgcga ggaggcccaa
     3061 ggcggggcca aggcttcaag ctctcaggac ttggctatgg aggaggagga agaaggcagg
     3121 agctcatcca gtccagcctt acctacagca ggaaactgca ccagcaccgg tggcggtggt
     3181 agcggcggtg gtggctctgg tggcggtggt tcaacgcgtg gtggcagcga agacgccaaa
     3241 aacataaaga aaggcccggc gccattctat cctctagagg atggaaccgc tggagagcaa
     3301 ctgcataagg ctatgaagag atacgccctg gttcctggaa caattgcttt tacagatgca
     3361 catatcgagg tgaacatcac gtacgcggaa tacttcgaaa tgtccgttcg gttggcagaa
     3421 gctatgaaac gatatgggct gaatacaaat cacagaatcg tcgtatgcag tgaaaactct
     3481 cttcaattct ttatgccggt gttgggcgcg ttatttatcg gagttgcagt tgcgcccgcg
     3541 aacgacattt ataatgaacg tgaattgctc aacagtatga acatttcgca gcctaccgta
     3601 gtgtttgttt ccaaaaaggg gttgcaaaaa attttgaacg tgcaaaaaaa attaccaata
     3661 atccagaaaa ttattatcat ggattctaaa acggattacc agggatttca gtcgatgtac
     3721 acgttcgtca catctcatct acctcccggt tttaatgaat acgattttgt accagagtcc
     3781 tttgatcgtg acaaaacaat tgcactgata atgaattcct ctggatctac tgggttacct
     3841 aagggtgtgg cccttccgca tagaactgcc tgcgtcagat tctcgcatgc cagagatcct
     3901 atttttggca atcaaatcat tccggatact gcgattttaa gtgttgttcc attccatcac
     3961 ggttttggaa tgtttactac actcggatat ttgatatgtg gatttcgagt cgtcttaatg
     4021 tatagatttg aagaagagct gtttttacga tcccttcagg attacaaaat tcaaagtgcg
     4081 ttgctagtac caaccctatt ttcattcttc gccaaaagca ctctgattga caaatacgat
     4141 ttatctaatt tacacgaaat tgcttctggg ggcgcacctc tttcgaaaga agtcggggaa
     4201 gcggttgcaa aacgcttcca tcttccaggg atacgacaag gatatgggct cactgagact
     4261 acatcagcta ttctgattac acccgagggg gatgataaac cgggcgcggt cggtaaagtt
     4321 gttccatttt ttgaagcgaa ggttgtggat ctggataccg ggaaaacgct gggcgttaat
     4381 cagagaggcg aattatgtgt cagaggacct atgattatgt ccggttatgt aaacaatccg
     4441 gaagcgacca acgccttgat tgacaaggat ggatggctac attctggaga catagcttac
     4501 tgggacgaag acgaacactt cttcatagtt gaccgcttga agtctttaat taaatacaaa
     4561 ggatatcagg tggcccccgc tgaattggaa tcgatattgt tacaacaccc caacatcttc
     4621 gacgcgggcg tggcaggtct tcccgacgat gacgccggtg aacttcccgc cgccgttgtt
     4681 gttttggagc acggaaagac gatgacggaa aaagagatcg tggattacgt cgccagtcaa
     4741 gtaacaaccg cgaaaaagtt gcgcggagga gttgtgtttg tggacgaagt accgaaaggt
     4801 cttaccggaa aactcgacgc aagaaaaatc agagagatcc tcataaaggc caagaagggc
     4861 ggaaagtcca aattgactag tgactacaaa gaccatgacg gtgattataa agatcatgac
     4921 atcgattaca aggatgacga tgacaagtta gatctaataa cttcgtatag catacattat
     4981 acgaagttat tttgaataaa atatctttat tttcattaca tctgtgtgtt ggttttttgt
     5041 gttgagctga tttaacaaaa atttaacgct gtggaatgtg tgtcagttag ggtgtggaaa
     5101 gtccccaggc tccccaggca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     5161 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     5221 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     5281 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     5341 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     5401 tttgcaaaaa gctattgtat atccattttc ggatctgatc agcactgagc aggtaccgcc
     5461 accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
     5521 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
     5581 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     5641 accctcgtga ccaccctgac ctggggcgtg cagtgcttcg cccgctaccc cgaccacatg
     5701 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
     5761 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     5821 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     5881 cacaagctgg agtacaacgc catcagcgac aacgtctata tcaccgccga caagcagaag
     5941 aacggcatca aggccaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc
     6001 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     6061 cactacctga gcacccagtc caagctgagc aaagacccca acgagaagcg cgatcacatg
     6121 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     6181 gaattcggaa gcggagctac taacttcagc ctgctgaagc aggctggaga cgtggaggag
     6241 aaccctggac ctcacgtggc caagcctttg tctcaagaag aatccaccct cattgaaaga
     6301 gcaacggcta caatcaacag catccccatc tctgaagact acagcgtcgc cagcgcagct
     6361 ctctctagcg acggccgcat cttcactggt gtcaatgtat atcattttac tgggggacct
     6421 tgtgcagaac tcgtggtgct gggcactgct gctgctgcgg cagctggcaa cctgacttgt
     6481 atcgtcgcga tcggaaatga gaacaggggc atcttgagcc cctgcggacg gtgccgacag
     6541 gtgcttctcg atctgcatcc tgggatcaaa gccatagtga aggacagtga tggacagccg
     6601 acggcagttg ggattcgtga attgctgccc tctggttatg tgtgggaggg ctaactcgag
     6661 accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     6721 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     6781 tatcttatca tgtctgtata gaagttccta ttccgaagtt cctattcttc aaatagtata
     6841 ggaacttcat aacttcgtat agcatacatt atacgaagtt atttgattaa ttaagcgatc
     6901 gcactccatt ctgggaccat ctcgaggagt cgatgagagg ctgtcttctc ctatgtccca
     6961 attctcagaa ctcagatgtg gctagaccaa ccagtgggac actgccccag cttctcccac
     7021 catagggggc cggaccccca tcaccagcct aggatccagg ggctgcctct ggcctcttag
     7081 ggagcagaga gcagaactcc gcagcccagc ccagaggagt gtcacctccc acctttggag
     7141 aggaatcctt ccctcccctg gacaaagttg ctgacaagct gctgaagtgg cctctccata
     7201 ttccagctga gcctgaatct gactcttgag ggttggggct gcacttattt attgcgggga
     7261 gacagctctc tctcccacct cctccccaga tgggaggaga gcctgaggcc caagcaggac
     7321 ccgggggttc cagcccctag ctgctctgga gtgggggagg ttggtggacc atggagtccc
     7381 tggtgctgcc cctcaggtgg gacccaggcg ttctcagctg taccctctgc cgatggcatt
     7441 tgtgtttttg atatttgtgt ctgttactac ttttttaata caaaaagata aaaacgccca
     7501 ggactttgtg gaatgaagtt tggggttggg gtgaggggag catgtcaagg ggagatgtgg
     7561 ccctgctttt gggaaggttg tagtgagggt gtacagggcc atctcctgac gacccccatt
     7621 cctcttttcc cccatcctgt ccaggtgagg gccctgctga gcccgctacg tccacgtcca
     7681 gctcagactc tgctgtcccc tctagcatgg cctcagctgg gtttgccctg ggaaggagag
     7741 ctgagaggtg cgtgtggcca agggcttggg taagctccag cccgggtcac gggtggccaa
     7801 ggcacctgca ggtcaattcc ctggcattat gcccagtaca tgaccttatg ggactttcct
     7861 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
     7921 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
     7981 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
     8041 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
     8101 agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc
     8161 catagaagac accgggaccg atccagcctg gggatccacc atgaaccggg gagtcccttt
     8221 taggcacttg cttctggtgc tgcaactggc gctcctccca gcagccactc agggaaagaa
     8281 agtggtgctg ggcaaaaaag gggatacagt ggaactgacc tgtacagctt cccagaagaa
     8341 gagcatacaa ttccactgga aaaactccaa ccagataaag attctgggaa atcagggctc
     8401 cttcttaact aaaggtccat ccaagctgaa tgatcgcgct gactcaagaa gaagcctttg
     8461 ggaccaagga aacttccccc tgatcatcaa gaatcttaag atagaagact cagatactta
     8521 catctgtgaa gtggaggacc agaaggagga ggtgcaattg ctagtgttcg gattgactgc
     8581 caactctgac acccacctgc ttcaggggca gagcctgacc ctgaccttgg agagcccccc
     8641 tggtagtagc ccctcagtgc aatgtaggag tccaaggggt aaaaacatac agggggggaa
     8701 gaccctctcc gtgtctcagc tggagctcca ggatagtggc acctggacat gcactgtctt
     8761 gcagaaccag aagaaggtgg agttcaaaat agacatcgtg gtgctagctt tccagaaggc
     8821 ctccagcata gtctataaga aagaggggga acaggtggag ttctccttcc cactcgcctt
     8881 tacagttgaa aagctgacgg gcagtggcga gctgtggtgg caggcggaga gggcttcctc
     8941 ctccaagtct tggatcacct ttgacctgaa gaacaaggaa gtgtctgtaa aacgggttac
     9001 ccaggaccct aagctccaga tgggcaagaa gctcccgctc cacctcaccc tgccccaggc
     9061 cttgcctcag tatgctggct ctggaaacct caccctggcc cttgaagcga aaacaggaaa
     9121 gttgcatcag gaagtgaacc tggtggtgat gagagccact cagctccaga aaaatttgac
     9181 ctgtgaggtg tggggaccca cctcccctaa gctgatgctg agcttgaaac tggagaacaa
     9241 ggaggcaaag gtctcgaagc gggagaaggc ggtgtgggtg ctgaaccctg aggcggggat
     9301 gtggcagtgt ctgctgagtg actcgggaca ggtcctgctg gaatccaaca tcaaggttct
     9361 gcccacatgg tcgaccccgg tgcagccaat ggccctgatt gtgctggggg gcgtcgccgg
     9421 cctcctgctt ttcattgggc taggcatctt cttctgtgtc aggtgccggc actgaaggcg
     9481 acctgcggat cccacgtcac tattgtatac tctatattat actctatgtt atactctgta
     9541 atcctactca ataaacgtgt cacgcctgtg aaaccgtact aagtctcccg tgtcttctta
     9601 tcaccatcag gtgacatcct cgcccaggct gtcaatcatg ccggtatcga ttccagtagc
     9661 accggcccca cgctgacaac ccactcttgc agcgttagca gcgcccctct taacaagccg
     9721 acccccacca gcgtcgcggt tactaacact cctctccccg acctgcagcc caagcttggc
     9781 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     9841 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     9901 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     9961 tt
//
