LOCUS       sequence-383166-        6204 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Red fluorescent, negative response-polarity voltage indicator under 
            the control of synapsin promoter; soma-targeted.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6204)
  AUTHORS   Kannan M, Vasan G, Haziza S, Huang C, Chrapkiewicz R, Luo J, Cardin 
            JA, Schnitzer MJ, Pieribone VA
  TITLE     Dual-polarity voltage imaging of the concurrent dynamics of multiple
            neuron types.
  JOURNAL   Science. 2022 Nov 4;378(6619):eabm8797. doi: 
            10.1126/science.abm8797. Epub 2022 Nov 4.
  PUBMED    36378956
REFERENCE   2  (bases 1 to 6204)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-11-4"; volume: "378(6619):eabm8797. doi"; pages: " 
            10.1126/science.abm8797. Epub 2022 Nov 4"
COMMENT     Sequence Label: pAAV-Syn-VARNAM2-Kv2.1PR
FEATURES             Location/Qualifiers
     source          1..6204
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        175..622
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      642..651
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    2331..2919
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2384..2404)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2802..2813)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2951..3427
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3150..3169)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3467..3607
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3467..3596
                     /label=AAV2 ITR
     rep_origin      3682..4137
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3769..3788)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3979..4000
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4154..4173)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4273..4295
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4333..4351)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4419..4523
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4524..4592,4593..5384)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4742..4761)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5555..6143
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6044..6063
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtgtct agactgcaga gggccctgcg tatgagtgca
      181 agtgggtttt aggaccagga tgaggcgggg tgggggtgcc tacctgacga ccgaccccga
      241 cccactggac aagcacccaa cccccattcc ccaaattgcg catcccctat cagagagggg
      301 gaggggaaac aggatgcggc gaggcgcgtg cgcactgcca gcttcagcac cgcggacagt
      361 gccttcgccc ccgcctggcg gcgcgcgcca ccgccgcctc agcactgaag gcgcgctgac
      421 gtcactcgcc ggtcccccgc aaactcccct tcccggccac cttggtcgcg tccgcgccgc
      481 cgccggccca gccggaccgc accacgcgag gcgcgagata ggggggcacg ggcgcgacca
      541 tctgcgctgc ggcgccggcg actcagcgct gcctcagtct gcggtgggca gcggaggagt
      601 cgtgtcgtgc ctgagagcgc agtcgagaag gtaccggatc cgccaccatg gctgacgtgg
      661 aaaccgagac cggcatgatt gcacagtgga ttgtctttgc tattatggct gctgctgcta
      721 ttgcttttgg agtggctgtg cactttcggc cttcagagct gaagagcgca tactatatca
      781 acattgccat ctgcactatc gccgctaccg cttactatgc aatggccgtg aactaccagg
      841 acctgacaat gaatggtgaa aggcaggtgg tctacgcaag atatattagc tgggtgctga
      901 ccacaccact gctcctgctc gatctcatcg tcatgaccaa gatgggcgga gtgatgattt
      961 cttgggtcat cggcgcagac attttcatga tcgtgtttgg tattctgggc gccttcgagg
     1021 atgaacacaa gttcaaatgg gtgtacttta tcgctggatg tgtgatgcag gcagtcctga
     1081 catacgggat gtataacgcc acttggaaag acgatctgaa gaaaagcccc gagtaccata
     1141 gctcctatgt cagtctgctc gtcttcctgt caatcctctg ggtgttttat cctgtcgtgt
     1201 gggctttcgg gtctggtagt ggcgtgctgt ccgtcgacaa tgaggccatt ctcatgggaa
     1261 tcctggatgt gctcgctaag ccactgtttg gaatggggtg cctcattgcc catgagacta
     1321 tcttcaagtc catcctgatt aaggaaaata tgcggatgaa ggtcgtgatg gaagggtctg
     1381 tcaatgggca ccagttcaag tgcaccggag agggagaggg caggccatac gagggcgtgc
     1441 agacaatgag gatcaaagtg atcgagggag gaccactgcc tttcgccttt gacatcctgg
     1501 ccaccagctt catgtacggc agcaggacct tcatcaagta tccagccgac atccccgatt
     1561 tctttaagca gagcttcccc gagggcttta cctgggagag ggtgacaaga tacgaggatg
     1621 gcggcgtggt gaccgtgaca caggacacct ctctggagga tggcgagctg gtgtataacg
     1681 tgaaggtgag gggcgtgaac ttccctagca atggcccagt gatgcagaag aagaccaagg
     1741 gctgggagcc caatacagag atgatgtacc ctgcagacgg aggcctgagg ggatataccg
     1801 acatcgccct gaaggtggat ggaggaggac acctgcactg taactttgtg accacatacc
     1861 gctccaagaa gacagtgggc aatatcaaga tgccaggagt gcacgccgtg gaccacaggc
     1921 tggagcgcat cgaggagtct gataacgaga catatgtggt gcagagagag gtggccgtgg
     1981 ccaagtactc taatctgggc ggcgggatgg acgagctgta taagaagagc aggatcacca
     2041 gcgagggcga gtacatcccc ctggaccaga tcgacatcaa cgtgttctgc tacgagaacg
     2101 aggtgcaaag tcagcctatc ctgaacacaa aggaaatggc tccacagtct aagcctcccg
     2161 aagagcttga gatgtccagt atgccaagtc ccgtggctcc cctccctgcc aggactgaag
     2221 gagtgattga catgaggagt atgtcatcta ttgatagctt catctcttgc gcaacagatt
     2281 tccccgaggc tactcgattc taataagaat tcgatatcaa gcttatcgat aatcaacctc
     2341 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     2401 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     2461 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     2521 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     2581 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     2641 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     2701 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctatgttg
     2761 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     2821 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     2881 ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccga gcgctgctcg
     2941 agagatctac gggtggcatc cctgtgaccc ctccccagtg cctctcctgg ccctggaagt
     3001 tgccactcca gtgcccacca gccttgtcct aataaaatta agttgcatca ttttgtctga
     3061 ctaggtgtcc ttctataata ttatggggtg gaggggggtg gtatggagca aggggcaagt
     3121 tgggaagaca acctgtaggg cctgcggggt ctattgggaa ccaagctgga gtgcagtggc
     3181 acaatcttgg ctcactgcaa tctccgcctc ctgggttcaa gcgattctcc tgcctcagcc
     3241 tcccgagttg ttgggattcc aggcatgcat gaccaggctc agctaatttt tgtttttttg
     3301 gtagagacgg ggtttcacca tattggccag gctggtctcc aactcctaat ctcaggtgat
     3361 ctacccacct tggcctccca aattgctggg attacaggcg tgaaccactg ctcccttccc
     3421 tgtccttctg attttgtagg taaccacgtg cggaccgagc ggccgcagga acccctagtg
     3481 atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag
     3541 gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc
     3601 ctgcaggggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
     3661 atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     3721 tggttacgcg cagcgtgacc gctacacttg ccagcgcctt agcgcccgct cctttcgctt
     3781 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     3841 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgatttgg
     3901 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     3961 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aactctatct
     4021 cgggctattc ttttgattta taagggattt tgccgatttc ggtctattgg ttaaaaaatg
     4081 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaattttat
     4141 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc
     4201 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
     4261 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
     4321 cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     4381 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     4441 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     4501 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     4561 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     4621 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     4681 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     4741 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     4801 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     4861 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     4921 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     4981 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     5041 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     5101 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     5161 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     5221 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     5281 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     5341 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     5401 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     5461 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     5521 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     5581 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     5641 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     5701 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     5761 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     5821 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     5881 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     5941 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     6001 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     6061 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     6121 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     6181 tttgctggcc ttttgctcac atgt
//
