LOCUS       sequence-383167-        6006 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Red fluorescent, negative response-polarity voltage indicator under 
            the control of synapsin promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6006)
  AUTHORS   Kannan M, Vasan G, Haziza S, Huang C, Chrapkiewicz R, Luo J, Cardin 
            JA, Schnitzer MJ, Pieribone VA
  TITLE     Dual-polarity voltage imaging of the concurrent dynamics of multiple
            neuron types.
  JOURNAL   Science. 2022 Nov 4;378(6619):eabm8797. doi: 
            10.1126/science.abm8797. Epub 2022 Nov 4.
  PUBMED    36378956
REFERENCE   2  (bases 1 to 6006)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-11-4"; volume: "378(6619):eabm8797. doi"; pages: " 
            10.1126/science.abm8797. Epub 2022 Nov 4"
COMMENT     Sequence Label: pAAV-Syn-VARNAM2
FEATURES             Location/Qualifiers
     source          1..6006
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        175..622
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      642..651
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    2133..2721
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2186..2206)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2604..2615)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2753..3229
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2952..2971)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3269..3409
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3269..3398
                     /label=AAV2 ITR
     rep_origin      3484..3939
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3571..3590)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3781..3802
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3956..3975)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4075..4097
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4135..4153)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4221..4325
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4326..4394,4395..5186)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4544..4563)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5357..5945
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5846..5865
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtgtct agactgcaga gggccctgcg tatgagtgca
      181 agtgggtttt aggaccagga tgaggcgggg tgggggtgcc tacctgacga ccgaccccga
      241 cccactggac aagcacccaa cccccattcc ccaaattgcg catcccctat cagagagggg
      301 gaggggaaac aggatgcggc gaggcgcgtg cgcactgcca gcttcagcac cgcggacagt
      361 gccttcgccc ccgcctggcg gcgcgcgcca ccgccgcctc agcactgaag gcgcgctgac
      421 gtcactcgcc ggtcccccgc aaactcccct tcccggccac cttggtcgcg tccgcgccgc
      481 cgccggccca gccggaccgc accacgcgag gcgcgagata ggggggcacg ggcgcgacca
      541 tctgcgctgc ggcgccggcg actcagcgct gcctcagtct gcggtgggca gcggaggagt
      601 cgtgtcgtgc ctgagagcgc agtcgagaag gtaccggatc cgccaccatg gctgacgtgg
      661 aaaccgagac cggcatgatt gcacagtgga ttgtctttgc tattatggct gctgctgcta
      721 ttgcttttgg agtggctgtg cactttcggc cttcagagct gaagagcgca tactatatca
      781 acattgccat ctgcactatc gccgctaccg cttactatgc aatggccgtg aactaccagg
      841 acctgacaat gaatggtgaa aggcaggtgg tctacgcaag atatattagc tgggtgctga
      901 ccacaccact gctcctgctc gatctcatcg tcatgaccaa gatgggcgga gtgatgattt
      961 cttgggtcat cggcgcagac attttcatga tcgtgtttgg tattctgggc gccttcgagg
     1021 atgaacacaa gttcaaatgg gtgtacttta tcgctggatg tgtgatgcag gcagtcctga
     1081 catacgggat gtataacgcc acttggaaag acgatctgaa gaaaagcccc gagtaccata
     1141 gctcctatgt cagtctgctc gtcttcctgt caatcctctg ggtgttttat cctgtcgtgt
     1201 gggctttcgg gtctggtagt ggcgtgctgt ccgtcgacaa tgaggccatt ctcatgggaa
     1261 tcctggatgt gctcgctaag ccactgtttg gaatggggtg cctcattgcc catgagacta
     1321 tcttcaagtc catcctgatt aaggaaaata tgcggatgaa ggtcgtgatg gaagggtctg
     1381 tcaatgggca ccagttcaag tgcaccggag agggagaggg caggccatac gagggcgtgc
     1441 agacaatgag gatcaaagtg atcgagggag gaccactgcc tttcgccttt gacatcctgg
     1501 ccaccagctt catgtacggc agcaggacct tcatcaagta tccagccgac atccccgatt
     1561 tctttaagca gagcttcccc gagggcttta cctgggagag ggtgacaaga tacgaggatg
     1621 gcggcgtggt gaccgtgaca caggacacct ctctggagga tggcgagctg gtgtataacg
     1681 tgaaggtgag gggcgtgaac ttccctagca atggcccagt gatgcagaag aagaccaagg
     1741 gctgggagcc caatacagag atgatgtacc ctgcagacgg aggcctgagg ggatataccg
     1801 acatcgccct gaaggtggat ggaggaggac acctgcactg taactttgtg accacatacc
     1861 gctccaagaa gacagtgggc aatatcaaga tgccaggagt gcacgccgtg gaccacaggc
     1921 tggagcgcat cgaggagtct gataacgaga catatgtggt gcagagagag gtggccgtgg
     1981 ccaagtactc taatctgggc ggcgggatgg acgagctgta taagaagagc aggatcacca
     2041 gcgagggcga gtacatcccc ctggaccaga tcgacatcaa cgtgttctgc tacgagaacg
     2101 aggtgtaaga attcgatatc aagcttatcg ataatcaacc tctggattac aaaatttgtg
     2161 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     2221 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     2281 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     2341 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     2401 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     2461 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     2521 cggggaaatc atcgtccttt ccttggctgc tcgcctatgt tgccacctgg attctgcgcg
     2581 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     2641 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     2701 ccctttgggc cgcctccccg catcgatacc gagcgctgct cgagagatct acgggtggca
     2761 tccctgtgac ccctccccag tgcctctcct ggccctggaa gttgccactc cagtgcccac
     2821 cagccttgtc ctaataaaat taagttgcat cattttgtct gactaggtgt ccttctataa
     2881 tattatgggg tggagggggg tggtatggag caaggggcaa gttgggaaga caacctgtag
     2941 ggcctgcggg gtctattggg aaccaagctg gagtgcagtg gcacaatctt ggctcactgc
     3001 aatctccgcc tcctgggttc aagcgattct cctgcctcag cctcccgagt tgttgggatt
     3061 ccaggcatgc atgaccaggc tcagctaatt tttgtttttt tggtagagac ggggtttcac
     3121 catattggcc aggctggtct ccaactccta atctcaggtg atctacccac cttggcctcc
     3181 caaattgctg ggattacagg cgtgaaccac tgctcccttc cctgtccttc tgattttgta
     3241 ggtaaccacg tgcggaccga gcggccgcag gaacccctag tgatggagtt ggccactccc
     3301 tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc
     3361 tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg
     3421 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca aagcaaccat
     3481 agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     3541 ccgctacact tgccagcgcc ttagcgcccg ctcctttcgc tttcttccct tcctttctcg
     3601 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     3661 ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg
     3721 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     3781 gtggactctt gttccaaact ggaacaacac tcaactctat ctcgggctat tcttttgatt
     3841 tataagggat tttgccgatt tcggtctatt ggttaaaaaa tgagctgatt taacaaaaat
     3901 ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact ctcagtacaa
     3961 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
     4021 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     4081 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
     4141 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     4201 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     4261 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     4321 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     4381 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     4441 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     4501 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     4561 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     4621 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     4681 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     4741 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     4801 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     4861 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     4921 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     4981 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     5041 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     5101 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     5161 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     5221 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     5281 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     5341 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     5401 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     5461 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     5521 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     5581 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     5641 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     5701 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     5761 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     5821 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     5881 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     5941 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     6001 acatgt
//
