LOCUS       sequence-383170-       10896 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing N-terminally tagged eGFP-TAX1BP1 Q770A 
            E774K.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10896)
  AUTHORS   Eapen VV, Swarup S, Hoyer MJ, Paulo JA, Harper JW
  TITLE     Quantitative proteomics reveals the selectivity of ubiquitin-binding
            autophagy receptors in the turnover of damaged lysosomes by 
            lysophagy.
  JOURNAL   eLife. 2021 Sep 29;10. pii: 72328. doi: 10.7554/eLife.72328.
  PUBMED    34585663
REFERENCE   2  (bases 1 to 10896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2021-09-29"; volume: "10. pii: 72328. doi"; pages: " 
            10.7554/eLife.72328"
COMMENT     Sequence Label: pHAGE-eGFP-TAX1BP1 Q770A E774K
FEATURES             Location/Qualifiers
     source          1..10896
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     505..529
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      588..597
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(594..596,597..599,600..1310)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(594..596,597..599,600..1310)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(639..660)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(900..919)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1247..1268
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    1326..1350
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3907..3931)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        3950..4449
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3985..4008)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4382..4401
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4473..4871
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    4913..5034
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4950..4969)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    5060..5648
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5113..5133)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5531..5542)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5723..5956
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6075..6093)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6161..6265
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6266..6334,6335..7126)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6484..6503)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7297..7885
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7786..7805
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8039..8056
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8296..8317
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8332..8337,8338..8355,8356..8362)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8370..8386
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8375..8397
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8394..8410
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8394..8410
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             8671..9304
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9351..9476
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9973..10206
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10391..10435
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10728..10845
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
      541 ccatccacgc tgttttgacc tccatagaag acaccggcgg ccgatctgcc accatggtga
      601 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
      661 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
      721 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
      781 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
      841 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
      901 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
      961 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     1021 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
     1081 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     1141 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     1201 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     1261 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag gctcgaggtt
     1321 atcaaacaag tttgtacaaa aaagttggca tgacatcctt tcaagaagtc ccattgcaga
     1381 cttccaactt tgcccatgtc atctttcaaa atgtggccaa gagttacctt cctaatgcac
     1441 acctggaatg tcattacacc ttaactccat atattcatcc acatccaaaa gattgggttg
     1501 gtatattcaa ggttggatgg agtactgctc gtgattatta cacgttttta tggtccccta
     1561 tgcctgaaca ttatgtggaa ggatcaacag tcaattgtgt actagcattc caaggatatt
     1621 accttccaaa tgatgatgga gaattttatc agttctgtta cgttacccat aagggtgaaa
     1681 ttcgtggagc aagtacacct ttccagtttc gagcttcttc tccagttgaa gagctgctta
     1741 ctatggaaga tgaaggaaat tctgacatgt tagtggtgac cacaaaagca ggccttcttg
     1801 agttgaaaat tgagaaaacc atgaaagaaa aagaagaact gttaaagtta attgccgttc
     1861 tggaaaaaga aacagcacaa cttcgagaac aagttgggag aatggaaaga gaacttaacc
     1921 atgagaaaga aagatgtgac caactgcaag cagaacaaaa gggtcttact gaagtaacac
     1981 aaagcttaaa aatggaaaat gaagagttta agaagaggtt cagtgatgct acatccaaag
     2041 cccatcagct tgaggaagat attgtgtcag taacacataa agcaattgaa aaagaaaccg
     2101 aattagacag tttaaaggac aaactcaaga aggcacaaca tgaaagagaa caacttgaat
     2161 gtcagttgaa gacagagaag gatgaaaagg aactttataa ggtacatttg aagaatacag
     2221 aaatagaaaa taccaagctt atgtcagagg tccagacttt aaaaaattta gatgggaaca
     2281 aagaaagcgt gattactcat ttcaaagaag agattggcag gctgcagtta tgtttggctg
     2341 aaaaggaaaa tctgcaaaga actttcctgc ttacaacctc aagtaaagaa gatacttgtt
     2401 ttttaaagga gcaacttcgt aaagcagagg aacaggttca ggcaactcgg caagaagttg
     2461 tctttctggc taaagaactc agtgatgctg tcaacgtacg agacagaacg atggcagacc
     2521 tgcatactgc acgcttggaa aacgagaaag tgaaaaagca gttagctgat gcagtggcag
     2581 aacttaaact aaatgctatg aaaaaagatc aggacaagac tgatacactg gaacacgaac
     2641 taagaagaga agttgaagat ctgaaactcc gtcttcagat ggctgcagac cattataaag
     2701 aaaaatttaa ggaatgccaa aggctccaaa aacaaataaa caaactttca gatcaatcag
     2761 ctaataataa taatgtcttc acaaagaaaa cggggaatca gcagaaagtg aatgatgctt
     2821 cagtaaacac agacccagcc acttctgcct ctactgtaga tgtaaagcca tcaccttctg
     2881 cagcagaggc agattttgac atagtaacaa aggggcaagt ctgtgaaatg accaaagaaa
     2941 ttgctgacaa aacagaaaag tataataaat gtaaacaact cttgcaggat gagaaagcaa
     3001 aatgcaataa atatgctgat gaacttgcaa aaatggagct gaaatggaaa gaacaagtga
     3061 aaattgctga aaatgtaaaa cttgaactag ctgaagtaca ggacaattat aaagaactta
     3121 aaaggagtct agaaaatcca gcagaaagga aaatggaagg tcagaattcc cagagtcctc
     3181 aatgtttcaa aacatgctca gagcaaaatg gttatgttct cacattgtca aatgcacaac
     3241 cagttctgca atatggtaat ccttatgcat ctcaggaaac aagagatgga gcagatggtg
     3301 ctttttaccc agatgaaata caaaggccac ctgtcagagt cccctcttgg ggactggaag
     3361 acaatgttgt ctgcagccag cctgctcgaa actttagtcg gcctgatggc ttagaggact
     3421 ctgaggatag caaagaagat gagaatgtgc ctactgctcc tgatcctcca agtcaacatt
     3481 tacgtgggca tgggacaggc ttttgctttg attccagctt tgatgttcac aagaagtgtc
     3541 ccctctgtga gttaatgttt cctcctaact atgatcagag caaatttgaa gaacatgttg
     3601 aaagtcactg gaaggtgtgc ccgatgtgca gcgagcagtt ccctcctgac tatgacgcgc
     3661 aggtgtttaa aaggcatgtg cagacccatt ttggcgagca gttccctcct gactatgacg
     3721 cgcaggtgtt taaaaggcat gtgcagaccc agcagttccc tcctgactat gacgcgcagg
     3781 tgtttaaaag gcatgtgcag acccattttg atcagaatgt tctacagttc cctcctgact
     3841 atgacgcgca ggtgtttaaa aggcatgtgc agacccattt tgatcagaat gttctaaatt
     3901 ttgactaccc aactttcttg tacaaagtgg ttcgataacg aattctaccg ggtaggggag
     3961 gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg cacttggcgc
     4021 tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg ccaaccggct
     4081 ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag gaagttcccc
     4141 cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg tctcactagt
     4201 ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg gggcagcggc
     4261 caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg tgggtccggg
     4321 ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc cggaggcccg
     4381 gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg
     4441 ggcctttcga cctgcagccc attaattaaa ccatggccaa gcctttgtct caagaagaat
     4501 ccaccctcat tgaaagagca acggctacaa tcaacagcat ccccatctct gaagactaca
     4561 gcgtcgccag cgcagctctc tctagcgacg gccgcatctt cactggtgtc aatgtatatc
     4621 attttactgg gggaccttgt gcagaactcg tggtgctggg cactgctgct gctgcggcag
     4681 ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc ttgagcccct
     4741 gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc atagtgaagg
     4801 acagtgatgg acagccgacg gcagttggga ttcgtgaatt gctgccctct ggttatgtgt
     4861 gggagggcta agcacttcgt ggccgaggag caggactgac actcgacctc gaaacttgtt
     4921 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     4981 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     5041 ctatgcatcg atagatccta atcaacctct ggattacaaa atttgtgaaa gattgactgg
     5101 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     5161 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     5221 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     5281 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     5341 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     5401 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     5461 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     5521 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     5581 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     5641 ctccccgcct gagatccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     5701 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg
     5761 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     5821 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     5881 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     5941 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca
     6001 aagaaatgaa tatcagagag tgagaggccc gggttaatta aggaaagggc tagatcattc
     6061 ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
     6121 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     6181 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     6241 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     6301 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     6361 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     6421 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     6481 tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg
     6541 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     6601 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     6661 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     6721 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     6781 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     6841 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     6901 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     6961 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     7021 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     7081 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     7141 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     7201 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     7261 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     7321 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     7381 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     7441 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     7501 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     7561 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     7621 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     7681 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     7741 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     7801 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     7861 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     7921 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     7981 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     8041 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     8101 ttggccgatt cattaatgca gcaagctcat ggctgactaa ttttttttat ttatgcagag
     8161 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     8221 ctaggctttt gcaaaaagct ccccgtggca cgacaggttt cccgactgga aagcgggcag
     8281 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
     8341 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
     8401 cagctatgac atgattacga atttcacaaa taaagcattt ttttcactgc attctagttg
     8461 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc
     8521 taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca attacctgtg
     8581 gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa gaaattgtat
     8641 ttgttaaata tgtactacaa acttagtagt tggaagggct aattcactcc caaagaagac
     8701 aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat tagcagaact
     8761 acacaccagg gccaggggtc agatatccac tgacctttgg atggtgctac aagctagtac
     8821 cagttgagcc agataaggta gaagaggcca ataaaggaga gaacaccagc ttgttacacc
     8881 ctgtgagcct gcatgggatg gatgacccgg agagagaagt gttagagtgg aggtttgaca
     8941 gccgcctagc atttcatcac gtggcccgag agctgcatcc ggagtacttc aagaactgct
     9001 gatatcgagc ttgctacaag ggactttccg ctggggactt tccagggagg cgtggcctgg
     9061 gcgggactgg ggagtggcga gccctcagat cctgcatata agcagctgct ttttgcctgt
     9121 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     9181 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     9241 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     9301 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
     9361 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
     9421 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
     9481 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
     9541 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
     9601 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
     9661 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
     9721 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
     9781 agcaaaacaa aagtaagacc accgcacagc aagcggccgg ccgctgatct tcagacctgg
     9841 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     9901 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     9961 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
    10021 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
    10081 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
    10141 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
    10201 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
    10261 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
    10321 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
    10381 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
    10441 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
    10501 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
    10561 atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga
    10621 aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg
    10681 atctcgacgg tatcgccgaa ttcacaaatg gcagtattca tccacaattt taaaagaaaa
    10741 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
    10801 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg
    10861 gacagcagag atccagtttg gactagtgga gttccg
//
