LOCUS       sequence-383199-        9203 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector to integrate HaloTag C-terminal into the human PER1 
            Locus to express it as a fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9203)
  AUTHORS   Gabriel CH, Del Olmo M, Rizki Widini A, Roshanbin R, Woyde J, Hamza 
            E, Gutu NN, Zehtabian A, Ewers H, Granada A, Herzel H, Kramer A
  TITLE     Circadian period is compensated for repressor protein turnover rates
            in single cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2024 Aug 20;121(34):e2404738121. doi: 
            10.1073/pnas.2404738121. Epub 2024 Aug 14.
  PUBMED    39141353
REFERENCE   2  (bases 1 to 9203)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2024-08-20"; volume: "121(34):e2404738121. 
            doi"; pages: " 10.1073/pnas.2404738121. Epub 2024 Aug 14"
COMMENT     Sequence Label: pDB2-hPER1-Halo-CD4-bla
FEATURES             Location/Qualifiers
     source          1..9203
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     41..59
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(97..119)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     219..238
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             1248..2135
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="AEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSY
                     VWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIH
                     DWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIID
                     QNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVE
                     EYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLI
                     GSEIARWLSTLEISG"
     CDS             2142..2207
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(2217..2250)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(2333..2353)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2338..2668
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2519..2654
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      2718..2727
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2724..2726,2727..2729,2730..3440)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2769..2790)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3030..3049)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3377..3398
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3456..3512
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3519..3914
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="AKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    3926..4047
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3963..3982)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4017..4036
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    4075..4108
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     protein_bind    complement(4109..4142)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        5163..5366
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5317..5337
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5363..5387
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(5461..5535,5536..6732)
                     /codon_start=1
                     /product="truncated human CD4 cell surface glycoprotein 
                     lacking the cytoplasmic tail"
                     /label=CD4 (truncated)
                     /translation="MNRGVPFRHLLLVLQLALLPAATQGKKVVLGKKGDTVELTCTASQ
                     KKSIQFHWKNSNQIKILGNQGSFLTKGPSKLNDRADSRRSLWDQGNFPLIIKNLKIEDS
                     DTYICEVEDQKEEVQLLVFGLTANSDTHLLQGQSLTLTLESPPGSSPSVQCRSPRGKNI
                     QGGKTLSVSQLELQDSGTWTCTVLQNQKKVEFKIDIVVLAFQKASSIVYKKEGEQVEFS
                     FPLAFTVEKLTGSGELWWQAERASSSKSWITFDLKNKEVSVKRVTQDPKLQMGKKLPLH
                     LTLPQALPQYAGSGNLTLALEAKTGKLHQEVNLVVMRATQLQKNLTCEVWGPTSPKLML
                     SLKLENKEAKVSKREKAVWVLNPEAGMWQCLLSDSGQVLLESNIKVLPTWSTPVQPMAL
                     IVLGGVAGLLLFIGLGIFFCVRCRH"
     primer_bind     complement(7050..7066)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7050..7066)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7063..7085)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7074..7090
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7098..7104,7105..7122,7123..7128))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7143..7164
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7281..7298)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7452..8040)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7532..7551)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8211..9002,9003..9071))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8834..8853
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9072..9176)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
       61 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
      121 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
      181 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact
      241 gagagtgcac catatgggcc ggcccttaag gtttaaactc tgaacccctt cttgggcagg
      301 gacatgacct ctgtgctgaa gcaggatcgg gagcggctcc gagccatgca gaagcagcag
      361 cctcggtttt ctgaggacca gcggcgggaa ctgggtgctg tgcactcctg ggtccggaag
      421 ggccaactgc ctcgggctct tgatgtgatg gtgagagaag cctgggacgg ggagaaaaaa
      481 gaattgagct caagttcaag ggggagaaaa aagaattgag ctcaagttca agggggagaa
      541 aaaagaattg agctcaagtt caagggatcg aggccaagag ctgatctcct tgatgtcctt
      601 ggatcattaa ttctgaagaa tgttgattcc actaaatttg ctgtggatta tagaatatta
      661 agccgcgtga gtctttgcag aacttttcac agcctatcct atgctaatat gcattgtgac
      721 tgtcctgtaa cggcatctgg gtagagggca caaggcactg tccaaccttg ttggaccgca
      781 ggtgcatctg tgtggactgg tgcttcttgg gagtacattt cgggaagcac agtgggctgg
      841 gggtgggaag ctgcgctggc aggttagcag tgagaaccct gtctgactct ctcatgtcca
      901 tttctctcac caaggcctgt gtggactgtg ggagcagcac ccaagatcct ggtcaccctg
      961 atgacccact cttctcagag ctggatggac tggggctgga gcccatggaa gagggtggag
     1021 gcgagcaggg cagcagcggt ggcggcagtg gtgagggaga gggctgcgag gaggcccaag
     1081 gcggggccaa ggcttcaagc tctcaggact tggctatgga ggaggaggaa gaaggcagga
     1141 gctcatccag tccagcctta cctacagcag gaaactgcac cagcaccggt ggcggtggta
     1201 gcggcggtgg tggctctggt ggcggtggtt caacgcgtgg tggcagcgca gaaatcggta
     1261 ctggctttcc attcgacccc cattatgtgg aagtcctggg cgagcgcatg cactacgtcg
     1321 atgttggtcc gcgcgatggc acccctgtgc tgttcctgca cggtaacccg acctcctcct
     1381 acgtgtggcg caacatcatc ccgcatgttg caccgaccca tcgctgcatt gctccagacc
     1441 tgatcggtat gggcaaatcc gacaaaccag acctgggtta tttcttcgac gaccacgtcc
     1501 gcttcatgga tgccttcatc gaagccctgg gtctggaaga ggtcgtcctg gtcattcacg
     1561 actggggctc cgctctgggt ttccactggg ccaagcgcaa tccagagcgc gtcaaaggta
     1621 ttgcatttat ggagttcatc cgccctatcc cgacctggga cgaatggcca gaatttgccc
     1681 gcgagacctt ccaggccttc cgcaccaccg acgtcggccg caagctgatc atcgatcaga
     1741 acgtttttat cgagggtacg ctgccgatgg gtgtcgtccg cccgctgact gaagtcgaga
     1801 tggaccatta ccgcgagccg ttcctgaatc ctgttgaccg cgagccactg tggcgcttcc
     1861 caaacgagct gccaatcgcc ggtgagccag cgaacatcgt cgcgctggtc gaagaataca
     1921 tggactggct gcaccagtcc cctgtcccga agctgctgtt ctggggcacc ccaggcgttc
     1981 tgatcccacc ggccgaagcc gctcgcctgg ccaaaagcct gcctaactgc aaggctgtgg
     2041 acatcggccc gggtctgaat ctgctgcaag aagacaaccc ggacctgatc ggcagcgaga
     2101 tcgcgcgctg gctgtcgacg ctcgagattt ccggtactag tgactacaaa gaccatgacg
     2161 gtgattataa agatcatgac atcgattaca aggatgacga tgacaagtta gatctaataa
     2221 cttcgtatag catacattat acgaagttat tttgaataaa atatctttat tttcattaca
     2281 tctgtgtgtt ggttttttgt gttgagctga tttaacaaaa atttaacgct gtggaatgtg
     2341 tgtcagttag ggtgtggaaa gtccccaggc tccccaggca ggcagaagta tgcaaagcat
     2401 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     2461 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     2521 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     2581 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg
     2641 cttttttgga ggcctaggct tttgcaaaaa gctattgtat atccattttc ggatctgatc
     2701 agcactgagc aggtaccgcc accatggtga gcaagggcga ggagctgttc accggggtgg
     2761 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
     2821 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
     2881 agctgcccgt gccctggccc accctcgtga ccaccctgac ctggggcgtg cagtgcttcg
     2941 cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     3001 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     3061 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     3121 aggacggcaa catcctgggg cacaagctgg agtacaacgc catcagcgac aacgtctata
     3181 tcaccgccga caagcagaag aacggcatca aggccaactt caagatccgc cacaacatcg
     3241 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     3301 ccgtgctgct gcccgacaac cactacctga gcacccagtc caagctgagc aaagacccca
     3361 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
     3421 gcatggacga gctgtacaag gaattcggaa gcggagctac taacttcagc ctgctgaagc
     3481 aggctggaga cgtggaggag aaccctggac ctcacgtggc caagcctttg tctcaagaag
     3541 aatccaccct cattgaaaga gcaacggcta caatcaacag catccccatc tctgaagact
     3601 acagcgtcgc cagcgcagct ctctctagcg acggccgcat cttcactggt gtcaatgtat
     3661 atcattttac tgggggacct tgtgcagaac tcgtggtgct gggcactgct gctgctgcgg
     3721 cagctggcaa cctgacttgt atcgtcgcga tcggaaatga gaacaggggc atcttgagcc
     3781 cctgcggacg gtgccgacag gtgcttctcg atctgcatcc tgggatcaaa gccatagtga
     3841 aggacagtga tggacagccg acggcagttg ggattcgtga attgctgccc tctggttatg
     3901 tgtgggaggg ctaactcgag accccaactt gtttattgca gcttataatg gttacaaata
     3961 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     4021 tttgtccaaa ctcatcaatg tatcttatca tgtctgtata gaagttccta ttccgaagtt
     4081 cctattcttc aaatagtata ggaacttcat aacttcgtat agcatacatt atacgaagtt
     4141 atttgattaa ttaagcgatc gcactccatt ctgggaccat ctcgaggagt cgatgagagg
     4201 ctgtcttctc ctatgtccca attctcagaa ctcagatgtg gctagaccaa ccagtgggac
     4261 actgccccag cttctcccac catagggggc cggaccccca tcaccagcct aggatccagg
     4321 ggctgcctct ggcctcttag ggagcagaga gcagaactcc gcagcccagc ccagaggagt
     4381 gtcacctccc acctttggag aggaatcctt ccctcccctg gacaaagttg ctgacaagct
     4441 gctgaagtgg cctctccata ttccagctga gcctgaatct gactcttgag ggttggggct
     4501 gcacttattt attgcgggga gacagctctc tctcccacct cctccccaga tgggaggaga
     4561 gcctgaggcc caagcaggac ccgggggttc cagcccctag ctgctctgga gtgggggagg
     4621 ttggtggacc atggagtccc tggtgctgcc cctcaggtgg gacccaggcg ttctcagctg
     4681 taccctctgc cgatggcatt tgtgtttttg atatttgtgt ctgttactac ttttttaata
     4741 caaaaagata aaaacgccca ggactttgtg gaatgaagtt tggggttggg gtgaggggag
     4801 catgtcaagg ggagatgtgg ccctgctttt gggaaggttg tagtgagggt gtacagggcc
     4861 atctcctgac gacccccatt cctcttttcc cccatcctgt ccaggtgagg gccctgctga
     4921 gcccgctacg tccacgtcca gctcagactc tgctgtcccc tctagcatgg cctcagctgg
     4981 gtttgccctg ggaaggagag ctgagaggtg cgtgtggcca agggcttggg taagctccag
     5041 cccgggtcac gggtggccaa ggcacctgca ggtcaattcc ctggcattat gcccagtaca
     5101 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
     5161 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
     5221 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
     5281 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
     5341 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc
     5401 atccacgctg ttttgacctc catagaagac accgggaccg atccagcctg gggatccacc
     5461 atgaaccggg gagtcccttt taggcacttg cttctggtgc tgcaactggc gctcctccca
     5521 gcagccactc agggaaagaa agtggtgctg ggcaaaaaag gggatacagt ggaactgacc
     5581 tgtacagctt cccagaagaa gagcatacaa ttccactgga aaaactccaa ccagataaag
     5641 attctgggaa atcagggctc cttcttaact aaaggtccat ccaagctgaa tgatcgcgct
     5701 gactcaagaa gaagcctttg ggaccaagga aacttccccc tgatcatcaa gaatcttaag
     5761 atagaagact cagatactta catctgtgaa gtggaggacc agaaggagga ggtgcaattg
     5821 ctagtgttcg gattgactgc caactctgac acccacctgc ttcaggggca gagcctgacc
     5881 ctgaccttgg agagcccccc tggtagtagc ccctcagtgc aatgtaggag tccaaggggt
     5941 aaaaacatac agggggggaa gaccctctcc gtgtctcagc tggagctcca ggatagtggc
     6001 acctggacat gcactgtctt gcagaaccag aagaaggtgg agttcaaaat agacatcgtg
     6061 gtgctagctt tccagaaggc ctccagcata gtctataaga aagaggggga acaggtggag
     6121 ttctccttcc cactcgcctt tacagttgaa aagctgacgg gcagtggcga gctgtggtgg
     6181 caggcggaga gggcttcctc ctccaagtct tggatcacct ttgacctgaa gaacaaggaa
     6241 gtgtctgtaa aacgggttac ccaggaccct aagctccaga tgggcaagaa gctcccgctc
     6301 cacctcaccc tgccccaggc cttgcctcag tatgctggct ctggaaacct caccctggcc
     6361 cttgaagcga aaacaggaaa gttgcatcag gaagtgaacc tggtggtgat gagagccact
     6421 cagctccaga aaaatttgac ctgtgaggtg tggggaccca cctcccctaa gctgatgctg
     6481 agcttgaaac tggagaacaa ggaggcaaag gtctcgaagc gggagaaggc ggtgtgggtg
     6541 ctgaaccctg aggcggggat gtggcagtgt ctgctgagtg actcgggaca ggtcctgctg
     6601 gaatccaaca tcaaggttct gcccacatgg tcgaccccgg tgcagccaat ggccctgatt
     6661 gtgctggggg gcgtcgccgg cctcctgctt ttcattgggc taggcatctt cttctgtgtc
     6721 aggtgccggc actgaaggcg acctgcggat cccacgtcac tattgtatac tctatattat
     6781 actctatgtt atactctgta atcctactca ataaacgtgt cacgcctgtg aaaccgtact
     6841 aagtctcccg tgtcttctta tcaccatcag gtgacatcct cgcccaggct gtcaatcatg
     6901 ccggtatcga ttccagtagc accggcccca cgctgacaac ccactcttgc agcgttagca
     6961 gcgcccctct taacaagccg acccccacca gcgtcgcggt tactaacact cctctccccg
     7021 acctgcagcc caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     7081 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     7141 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     7201 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     7261 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     7321 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     7381 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     7441 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     7501 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     7561 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     7621 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     7681 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     7741 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     7801 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     7861 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     7921 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     7981 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     8041 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     8101 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     8161 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     8221 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     8281 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     8341 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     8401 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     8461 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     8521 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     8581 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     8641 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     8701 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     8761 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     8821 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     8881 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     8941 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     9001 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     9061 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     9121 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     9181 tttccccgaa aagtgccacc tga
//
