LOCUS       sequence-383200-       10064 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector to integrate firefly Luciferase C-terminal into the 
            human CRY2 Locus to express it as a fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10064)
  AUTHORS   Gabriel CH, Del Olmo M, Rizki Widini A, Roshanbin R, Woyde J, Hamza 
            E, Gutu NN, Zehtabian A, Ewers H, Granada A, Herzel H, Kramer A
  TITLE     Circadian period is compensated for repressor protein turnover rates
            in single cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2024 Aug 20;121(34):e2404738121. doi: 
            10.1073/pnas.2404738121. Epub 2024 Aug 14.
  PUBMED    39141353
REFERENCE   2  (bases 1 to 10064)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2024-08-20"; volume: "121(34):e2404738121. 
            doi"; pages: " 10.1073/pnas.2404738121. Epub 2024 Aug 14"
COMMENT     Sequence Label: pDB2-hCRY2-Luciferase-CD4-bla
FEATURES             Location/Qualifiers
     source          1..10064
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     65..83
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     243..262
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             1277..2923
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="EDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAH
                     IEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPA
                     NDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSM
                     YTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHAR
                     DPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKI
                     QSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYG
                     LTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSG
                     YVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILL
                     QHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVF
                     VDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(1340..1358)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     CDS             2930..2995
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(3005..3038)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(3121..3141)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3126..3456
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3307..3442
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3369..3388
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     regulatory      3506..3515
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3512..3514,3515..3517,3518..4228)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3557..3578)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3818..3837)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4165..4186
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4244..4300
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4307..4702
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="AKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    4714..4835
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4751..4770)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4805..4824
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    4863..4896
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     protein_bind    complement(4897..4930)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        6048..6251
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6202..6222
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6248..6272
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(6346..6420,6421..7617)
                     /codon_start=1
                     /product="truncated human CD4 cell surface glycoprotein 
                     lacking the cytoplasmic tail"
                     /label=CD4 (truncated)
                     /translation="MNRGVPFRHLLLVLQLALLPAATQGKKVVLGKKGDTVELTCTASQ
                     KKSIQFHWKNSNQIKILGNQGSFLTKGPSKLNDRADSRRSLWDQGNFPLIIKNLKIEDS
                     DTYICEVEDQKEEVQLLVFGLTANSDTHLLQGQSLTLTLESPPGSSPSVQCRSPRGKNI
                     QGGKTLSVSQLELQDSGTWTCTVLQNQKKVEFKIDIVVLAFQKASSIVYKKEGEQVEFS
                     FPLAFTVEKLTGSGELWWQAERASSSKSWITFDLKNKEVSVKRVTQDPKLQMGKKLPLH
                     LTLPQALPQYAGSGNLTLALEAKTGKLHQEVNLVVMRATQLQKNLTCEVWGPTSPKLML
                     SLKLENKEAKVSKREKAVWVLNPEAGMWQCLLSDSGQVLLESNIKVLPTWSTPVQPMAL
                     IVLGGVAGLLLFIGLGIFFCVRCRH"
     primer_bind     complement(7935..7951)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7935..7951)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7948..7970)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7959..7975
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7983..7989,7990..8007,8008..8013))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8028..8049
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8166..8183)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8337..8925)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8417..8436)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9096..9887,9888..9956))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9719..9738
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9957..10061)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
       61 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
      121 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
      181 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
      241 tgcggcatca gagcagattg tactgagagt gcaccatatg ggccggccct taaggtttaa
      301 acaggaaccg aagggaggtc agtgtagcgg catgtcccag tgaggagctg gaatgagatg
      361 aggacaagat acagcatgag cagcatgtgg caggcctgat gtccgagacg aggactagga
      421 ttcagccttc agtgcactga aggatggtca gtagggcagg gcatgattgg acttaggtct
      481 gaaaaagctc cctggcctgc tgggtggaga ttggacagtg taggccggag cgggcacagg
      541 gaggccagag aggaggcagc acagacaggg tggtggccgc aaggagaaag actggacaga
      601 ttcgctgcat tcggagcctc ttcccttctt ggggatgaag atgagtaaca tgagatggga
      661 agaacacggg tttttctagt tggcccagct aggctgcctc catgggcagc atgactcctc
      721 cacaggggcc ctgctctttg ctgcttgggg aggctgattc agggttgcag agggaatgcc
      781 tcaccttcaa agcaggacag caggcaggcc aaatcagtct ctggccacag cccatgccat
      841 ttggggccca aaagctggtc ctgctaccag caggcctgct tggtcatgtc ctccttggac
      901 atttccaggc aaagtcactg ctgaaagcaa ggccgagggg caggtctgag gagcagcatg
      961 ctggcattcc attgataagt gtgctccctg tcccctaaag agctgctgct gcatagtgtg
     1021 ggatcatttt ggctgcatgc acagtctgtg tgaccctttg acacgcttcc ctacaggccc
     1081 aagaccacta cccagtggcc cagcatcccc caaacgcaag ctggaagcag ccgaggaacc
     1141 acctggtgaa gaactcagca aacgggcccg ggtggcagag ttgccaaccc cagagctgcc
     1201 gagcaaggat gccaccggtg gcggtggtag cggcggtggt ggctctggtg gcggtggttc
     1261 aacgcgtggt ggcagcgaag acgccaaaaa cataaagaaa ggcccggcgc cattctatcc
     1321 tctagaggat ggaaccgctg gagagcaact gcataaggct atgaagagat acgccctggt
     1381 tcctggaaca attgctttta cagatgcaca tatcgaggtg aacatcacgt acgcggaata
     1441 cttcgaaatg tccgttcggt tggcagaagc tatgaaacga tatgggctga atacaaatca
     1501 cagaatcgtc gtatgcagtg aaaactctct tcaattcttt atgccggtgt tgggcgcgtt
     1561 atttatcgga gttgcagttg cgcccgcgaa cgacatttat aatgaacgtg aattgctcaa
     1621 cagtatgaac atttcgcagc ctaccgtagt gtttgtttcc aaaaaggggt tgcaaaaaat
     1681 tttgaacgtg caaaaaaaat taccaataat ccagaaaatt attatcatgg attctaaaac
     1741 ggattaccag ggatttcagt cgatgtacac gttcgtcaca tctcatctac ctcccggttt
     1801 taatgaatac gattttgtac cagagtcctt tgatcgtgac aaaacaattg cactgataat
     1861 gaattcctct ggatctactg ggttacctaa gggtgtggcc cttccgcata gaactgcctg
     1921 cgtcagattc tcgcatgcca gagatcctat ttttggcaat caaatcattc cggatactgc
     1981 gattttaagt gttgttccat tccatcacgg ttttggaatg tttactacac tcggatattt
     2041 gatatgtgga tttcgagtcg tcttaatgta tagatttgaa gaagagctgt ttttacgatc
     2101 ccttcaggat tacaaaattc aaagtgcgtt gctagtacca accctatttt cattcttcgc
     2161 caaaagcact ctgattgaca aatacgattt atctaattta cacgaaattg cttctggggg
     2221 cgcacctctt tcgaaagaag tcggggaagc ggttgcaaaa cgcttccatc ttccagggat
     2281 acgacaagga tatgggctca ctgagactac atcagctatt ctgattacac ccgaggggga
     2341 tgataaaccg ggcgcggtcg gtaaagttgt tccatttttt gaagcgaagg ttgtggatct
     2401 ggataccggg aaaacgctgg gcgttaatca gagaggcgaa ttatgtgtca gaggacctat
     2461 gattatgtcc ggttatgtaa acaatccgga agcgaccaac gccttgattg acaaggatgg
     2521 atggctacat tctggagaca tagcttactg ggacgaagac gaacacttct tcatagttga
     2581 ccgcttgaag tctttaatta aatacaaagg atatcaggtg gcccccgctg aattggaatc
     2641 gatattgtta caacacccca acatcttcga cgcgggcgtg gcaggtcttc ccgacgatga
     2701 cgccggtgaa cttcccgccg ccgttgttgt tttggagcac ggaaagacga tgacggaaaa
     2761 agagatcgtg gattacgtcg ccagtcaagt aacaaccgcg aaaaagttgc gcggaggagt
     2821 tgtgtttgtg gacgaagtac cgaaaggtct taccggaaaa ctcgacgcaa gaaaaatcag
     2881 agagatcctc ataaaggcca agaagggcgg aaagtccaaa ttgactagtg actacaaaga
     2941 ccatgacggt gattataaag atcatgacat cgattacaag gatgacgatg acaagttaga
     3001 tctaataact tcgtatagca tacattatac gaagttattt tgaataaaat atctttattt
     3061 tcattacatc tgtgtgttgg ttttttgtgt tgagctgatt taacaaaaat ttaacgctgt
     3121 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccaggcagg cagaagtatg
     3181 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     3241 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     3301 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     3361 atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag
     3421 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tattgtatat ccattttcgg
     3481 atctgatcag cactgagcag gtaccgccac catggtgagc aagggcgagg agctgttcac
     3541 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     3601 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
     3661 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct ggggcgtgca
     3721 gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     3781 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     3841 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     3901 cttcaaggag gacggcaaca tcctggggca caagctggag tacaacgcca tcagcgacaa
     3961 cgtctatatc accgccgaca agcagaagaa cggcatcaag gccaacttca agatccgcca
     4021 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     4081 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtcca agctgagcaa
     4141 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     4201 cactctcggc atggacgagc tgtacaagga attcggaagc ggagctacta acttcagcct
     4261 gctgaagcag gctggagacg tggaggagaa ccctggacct cacgtggcca agcctttgtc
     4321 tcaagaagaa tccaccctca ttgaaagagc aacggctaca atcaacagca tccccatctc
     4381 tgaagactac agcgtcgcca gcgcagctct ctctagcgac ggccgcatct tcactggtgt
     4441 caatgtatat cattttactg ggggaccttg tgcagaactc gtggtgctgg gcactgctgc
     4501 tgctgcggca gctggcaacc tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat
     4561 cttgagcccc tgcggacggt gccgacaggt gcttctcgat ctgcatcctg ggatcaaagc
     4621 catagtgaag gacagtgatg gacagccgac ggcagttggg attcgtgaat tgctgccctc
     4681 tggttatgtg tgggagggct aactcgagac cccaacttgt ttattgcagc ttataatggt
     4741 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     4801 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtataga agttcctatt
     4861 ccgaagttcc tattcttcaa atagtatagg aacttcataa cttcgtatag catacattat
     4921 acgaagttat ttgattaatt aagcgatcgc ggatgcctga gagtgagtga cagcagccta
     4981 gattcaacct cacgaacgaa gttgggagtg ggggggccta ctgccctgcc agctgcaggt
     5041 tgacacatag cacactagat gaaaatctgg tgggaccacc caatgctcag ccactaaagc
     5101 tttaccattt tacagtggca gccgggttcc atcctggctt agggaatgag tactttctgg
     5161 ttgatatctg tgtgaccttt accattgggc cggctatggt ggctcatgta tgtaatccca
     5221 gcactttggg aggccaaggt gggaggatcg cttgaggcca ggagtacaag accaacctgg
     5281 ccaaaccaac atagtgagac tccatctcta aaaacataat aataataata ataataatct
     5341 ggtgggactg catgactttt aggatttcag ctcaaaaatc ccaccattct gacttttatc
     5401 agagatttaa aagtcaagcc cttttgtatt tttttgccag ctaagggaaa aagggaggca
     5461 tgggggctga ggggtggcgc tttctaccta ttaaaatgag tcacactcaa gagaaagaaa
     5521 gctgagacct gcgtgtgaag cagcggggaa aaggcgcatt gcagggtctg aggctgctcc
     5581 aggccccctg ctttccgaca ggctgtgttc tataggccag ccagtggagg gcagcactga
     5641 ccttgagaag cctggacaga gggctgtgct gtgtcttact tggcccagcc ctgcctgagg
     5701 accagtagac ccagtctgtc atcatctttg ccttttgcgg cctctcctcc cttctcaccc
     5761 atcctgtggc ctcagctctg cacctccctg gcagcgctca tcttggaagc ctcctgaatg
     5821 tcatcaaaac actggcttct cctttcccaa gggaggaagg gccttttatg ccactcttgg
     5881 ggaaatgtaa attcctgtcc cctctgagtc atcatctcaa gctgttcctt atgcaccatg
     5941 tcttcgtcac ctgcaggtca attccctggc attatgccca gtacatgacc ttatgggact
     6001 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     6061 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     6121 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     6181 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     6241 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
     6301 acctccatag aagacaccgg gaccgatcca gcctggggat ccaccatgaa ccggggagtc
     6361 ccttttaggc acttgcttct ggtgctgcaa ctggcgctcc tcccagcagc cactcaggga
     6421 aagaaagtgg tgctgggcaa aaaaggggat acagtggaac tgacctgtac agcttcccag
     6481 aagaagagca tacaattcca ctggaaaaac tccaaccaga taaagattct gggaaatcag
     6541 ggctccttct taactaaagg tccatccaag ctgaatgatc gcgctgactc aagaagaagc
     6601 ctttgggacc aaggaaactt ccccctgatc atcaagaatc ttaagataga agactcagat
     6661 acttacatct gtgaagtgga ggaccagaag gaggaggtgc aattgctagt gttcggattg
     6721 actgccaact ctgacaccca cctgcttcag gggcagagcc tgaccctgac cttggagagc
     6781 ccccctggta gtagcccctc agtgcaatgt aggagtccaa ggggtaaaaa catacagggg
     6841 gggaagaccc tctccgtgtc tcagctggag ctccaggata gtggcacctg gacatgcact
     6901 gtcttgcaga accagaagaa ggtggagttc aaaatagaca tcgtggtgct agctttccag
     6961 aaggcctcca gcatagtcta taagaaagag ggggaacagg tggagttctc cttcccactc
     7021 gcctttacag ttgaaaagct gacgggcagt ggcgagctgt ggtggcaggc ggagagggct
     7081 tcctcctcca agtcttggat cacctttgac ctgaagaaca aggaagtgtc tgtaaaacgg
     7141 gttacccagg accctaagct ccagatgggc aagaagctcc cgctccacct caccctgccc
     7201 caggccttgc ctcagtatgc tggctctgga aacctcaccc tggcccttga agcgaaaaca
     7261 ggaaagttgc atcaggaagt gaacctggtg gtgatgagag ccactcagct ccagaaaaat
     7321 ttgacctgtg aggtgtgggg acccacctcc cctaagctga tgctgagctt gaaactggag
     7381 aacaaggagg caaaggtctc gaagcgggag aaggcggtgt gggtgctgaa ccctgaggcg
     7441 gggatgtggc agtgtctgct gagtgactcg ggacaggtcc tgctggaatc caacatcaag
     7501 gttctgccca catggtcgac cccggtgcag ccaatggccc tgattgtgct ggggggcgtc
     7561 gccggcctcc tgcttttcat tgggctaggc atcttcttct gtgtcaggtg ccggcactga
     7621 aggcgacctg cggatcccac gtcactattg tatactctat attatactct atgttatact
     7681 ctgtaatcct actcaataaa cgtgtcacgc ctgtgaaacc gtactaagtc tcccgtgtct
     7741 tcttatcacc atcaggtgac atcctcgccc aggctgtcaa tcatgccggt atcgattcca
     7801 gtagcaccgg ccccacgctg acaacccact cttgcagcgt tagcagcgcc cctcttaaca
     7861 agccgacccc caccagcgtc gcggttacta acactcctct ccccgacctg cagcccaagc
     7921 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     7981 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     8041 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     8101 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     8161 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     8221 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     8281 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     8341 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     8401 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     8461 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     8521 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     8581 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     8641 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     8701 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     8761 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     8821 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     8881 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     8941 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     9001 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     9061 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     9121 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     9181 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     9241 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     9301 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     9361 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     9421 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     9481 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     9541 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     9601 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     9661 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     9721 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     9781 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     9841 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     9901 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     9961 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
    10021 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcac
//
