LOCUS       sequence-383203-        9305 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector to integrate HaloTag C-terminal into the human CRY2 
            Locus to express it as a fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9305)
  AUTHORS   Gabriel CH, Del Olmo M, Rizki Widini A, Roshanbin R, Woyde J, Hamza 
            E, Gutu NN, Zehtabian A, Ewers H, Granada A, Herzel H, Kramer A
  TITLE     Circadian period is compensated for repressor protein turnover rates
            in single cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2024 Aug 20;121(34):e2404738121. doi: 
            10.1073/pnas.2404738121. Epub 2024 Aug 14.
  PUBMED    39141353
REFERENCE   2  (bases 1 to 9305)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2024-08-20"; volume: "121(34):e2404738121. 
            doi"; pages: " 10.1073/pnas.2404738121. Epub 2024 Aug 14"
COMMENT     Sequence Label: pDB2-hCRY2-Halo-CD4-bla
FEATURES             Location/Qualifiers
     source          1..9305
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     29..47
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(85..107)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     207..226
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             1241..2128
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="AEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSY
                     VWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIH
                     DWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIID
                     QNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVE
                     EYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLI
                     GSEIARWLSTLEISG"
     CDS             2135..2200
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(2210..2243)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(2326..2346)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2331..2661
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2512..2647
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2574..2593
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     regulatory      2711..2720
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2717..2719,2720..2722,2723..3433)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2762..2783)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3023..3042)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3370..3391
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3449..3505
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3512..3907
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="AKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    3919..4040
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3956..3975)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4010..4029
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    4068..4101
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     protein_bind    complement(4102..4135)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        5253..5456
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5407..5427
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5453..5477
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(5551..5625,5626..6822)
                     /codon_start=1
                     /product="truncated human CD4 cell surface glycoprotein 
                     lacking the cytoplasmic tail"
                     /label=CD4 (truncated)
                     /translation="MNRGVPFRHLLLVLQLALLPAATQGKKVVLGKKGDTVELTCTASQ
                     KKSIQFHWKNSNQIKILGNQGSFLTKGPSKLNDRADSRRSLWDQGNFPLIIKNLKIEDS
                     DTYICEVEDQKEEVQLLVFGLTANSDTHLLQGQSLTLTLESPPGSSPSVQCRSPRGKNI
                     QGGKTLSVSQLELQDSGTWTCTVLQNQKKVEFKIDIVVLAFQKASSIVYKKEGEQVEFS
                     FPLAFTVEKLTGSGELWWQAERASSSKSWITFDLKNKEVSVKRVTQDPKLQMGKKLPLH
                     LTLPQALPQYAGSGNLTLALEAKTGKLHQEVNLVVMRATQLQKNLTCEVWGPTSPKLML
                     SLKLENKEAKVSKREKAVWVLNPEAGMWQCLLSDSGQVLLESNIKVLPTWSTPVQPMAL
                     IVLGGVAGLLLFIGLGIFFCVRCRH"
     primer_bind     complement(7140..7156)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7140..7156)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7153..7175)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7164..7180
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7188..7194,7195..7212,7213..7218))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7233..7254
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7371..7388)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7542..8130)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7622..7641)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8301..9092,9093..9161))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8924..8943
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9162..9266)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc
       61 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
      121 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
      181 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
      241 tatgggccgg cccttaaggt ttaaacagga accgaaggga ggtcagtgta gcggcatgtc
      301 ccagtgagga gctggaatga gatgaggaca agatacagca tgagcagcat gtggcaggcc
      361 tgatgtccga gacgaggact aggattcagc cttcagtgca ctgaaggatg gtcagtaggg
      421 cagggcatga ttggacttag gtctgaaaaa gctccctggc ctgctgggtg gagattggac
      481 agtgtaggcc ggagcgggca cagggaggcc agagaggagg cagcacagac agggtggtgg
      541 ccgcaaggag aaagactgga cagattcgct gcattcggag cctcttccct tcttggggat
      601 gaagatgagt aacatgagat gggaagaaca cgggtttttc tagttggccc agctaggctg
      661 cctccatggg cagcatgact cctccacagg ggccctgctc tttgctgctt ggggaggctg
      721 attcagggtt gcagagggaa tgcctcacct tcaaagcagg acagcaggca ggccaaatca
      781 gtctctggcc acagcccatg ccatttgggg cccaaaagct ggtcctgcta ccagcaggcc
      841 tgcttggtca tgtcctcctt ggacatttcc aggcaaagtc actgctgaaa gcaaggccga
      901 ggggcaggtc tgaggagcag catgctggca ttccattgat aagtgtgctc cctgtcccct
      961 aaagagctgc tgctgcatag tgtgggatca ttttggctgc atgcacagtc tgtgtgaccc
     1021 tttgacacgc ttccctacag gcccaagacc actacccagt ggcccagcat cccccaaacg
     1081 caagctggaa gcagccgagg aaccacctgg tgaagaactc agcaaacggg cccgggtggc
     1141 agagttgcca accccagagc tgccgagcaa ggatgccacc ggtggcggtg gtagcggcgg
     1201 tggtggctct ggtggcggtg gttcaacgcg tggtggcagc gcagaaatcg gtactggctt
     1261 tccattcgac ccccattatg tggaagtcct gggcgagcgc atgcactacg tcgatgttgg
     1321 tccgcgcgat ggcacccctg tgctgttcct gcacggtaac ccgacctcct cctacgtgtg
     1381 gcgcaacatc atcccgcatg ttgcaccgac ccatcgctgc attgctccag acctgatcgg
     1441 tatgggcaaa tccgacaaac cagacctggg ttatttcttc gacgaccacg tccgcttcat
     1501 ggatgccttc atcgaagccc tgggtctgga agaggtcgtc ctggtcattc acgactgggg
     1561 ctccgctctg ggtttccact gggccaagcg caatccagag cgcgtcaaag gtattgcatt
     1621 tatggagttc atccgcccta tcccgacctg ggacgaatgg ccagaatttg cccgcgagac
     1681 cttccaggcc ttccgcacca ccgacgtcgg ccgcaagctg atcatcgatc agaacgtttt
     1741 tatcgagggt acgctgccga tgggtgtcgt ccgcccgctg actgaagtcg agatggacca
     1801 ttaccgcgag ccgttcctga atcctgttga ccgcgagcca ctgtggcgct tcccaaacga
     1861 gctgccaatc gccggtgagc cagcgaacat cgtcgcgctg gtcgaagaat acatggactg
     1921 gctgcaccag tcccctgtcc cgaagctgct gttctggggc accccaggcg ttctgatccc
     1981 accggccgaa gccgctcgcc tggccaaaag cctgcctaac tgcaaggctg tggacatcgg
     2041 cccgggtctg aatctgctgc aagaagacaa cccggacctg atcggcagcg agatcgcgcg
     2101 ctggctgtcg acgctcgaga tttccggtac tagtgactac aaagaccatg acggtgatta
     2161 taaagatcat gacatcgatt acaaggatga cgatgacaag ttagatctaa taacttcgta
     2221 tagcatacat tatacgaagt tattttgaat aaaatatctt tattttcatt acatctgtgt
     2281 gttggttttt tgtgttgagc tgatttaaca aaaatttaac gctgtggaat gtgtgtcagt
     2341 tagggtgtgg aaagtcccca ggctccccag gcaggcagaa gtatgcaaag catgcatctc
     2401 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     2461 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     2521 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     2581 gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt
     2641 ggaggcctag gcttttgcaa aaagctattg tatatccatt ttcggatctg atcagcactg
     2701 agcaggtacc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
     2761 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
     2821 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     2881 cgtgccctgg cccaccctcg tgaccaccct gacctggggc gtgcagtgct tcgcccgcta
     2941 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     3001 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     3061 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     3121 caacatcctg gggcacaagc tggagtacaa cgccatcagc gacaacgtct atatcaccgc
     3181 cgacaagcag aagaacggca tcaaggccaa cttcaagatc cgccacaaca tcgaggacgg
     3241 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     3301 gctgcccgac aaccactacc tgagcaccca gtccaagctg agcaaagacc ccaacgagaa
     3361 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     3421 cgagctgtac aaggaattcg gaagcggagc tactaacttc agcctgctga agcaggctgg
     3481 agacgtggag gagaaccctg gacctcacgt ggccaagcct ttgtctcaag aagaatccac
     3541 cctcattgaa agagcaacgg ctacaatcaa cagcatcccc atctctgaag actacagcgt
     3601 cgccagcgca gctctctcta gcgacggccg catcttcact ggtgtcaatg tatatcattt
     3661 tactggggga ccttgtgcag aactcgtggt gctgggcact gctgctgctg cggcagctgg
     3721 caacctgact tgtatcgtcg cgatcggaaa tgagaacagg ggcatcttga gcccctgcgg
     3781 acggtgccga caggtgcttc tcgatctgca tcctgggatc aaagccatag tgaaggacag
     3841 tgatggacag ccgacggcag ttgggattcg tgaattgctg ccctctggtt atgtgtggga
     3901 gggctaactc gagaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     3961 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     4021 aaactcatca atgtatctta tcatgtctgt atagaagttc ctattccgaa gttcctattc
     4081 ttcaaatagt ataggaactt cataacttcg tatagcatac attatacgaa gttatttgat
     4141 taattaagcg atcgcggatg cctgagagtg agtgacagca gcctagattc aacctcacga
     4201 acgaagttgg gagtgggggg gcctactgcc ctgccagctg caggttgaca catagcacac
     4261 tagatgaaaa tctggtggga ccacccaatg ctcagccact aaagctttac cattttacag
     4321 tggcagccgg gttccatcct ggcttaggga atgagtactt tctggttgat atctgtgtga
     4381 cctttaccat tgggccggct atggtggctc atgtatgtaa tcccagcact ttgggaggcc
     4441 aaggtgggag gatcgcttga ggccaggagt acaagaccaa cctggccaaa ccaacatagt
     4501 gagactccat ctctaaaaac ataataataa taataataat aatctggtgg gactgcatga
     4561 cttttaggat ttcagctcaa aaatcccacc attctgactt ttatcagaga tttaaaagtc
     4621 aagccctttt gtattttttt gccagctaag ggaaaaaggg aggcatgggg gctgaggggt
     4681 ggcgctttct acctattaaa atgagtcaca ctcaagagaa agaaagctga gacctgcgtg
     4741 tgaagcagcg gggaaaaggc gcattgcagg gtctgaggct gctccaggcc ccctgctttc
     4801 cgacaggctg tgttctatag gccagccagt ggagggcagc actgaccttg agaagcctgg
     4861 acagagggct gtgctgtgtc ttacttggcc cagccctgcc tgaggaccag tagacccagt
     4921 ctgtcatcat ctttgccttt tgcggcctct cctcccttct cacccatcct gtggcctcag
     4981 ctctgcacct ccctggcagc gctcatcttg gaagcctcct gaatgtcatc aaaacactgg
     5041 cttctccttt cccaagggag gaagggcctt ttatgccact cttggggaaa tgtaaattcc
     5101 tgtcccctct gagtcatcat ctcaagctgt tccttatgca ccatgtcttc gtcacctgca
     5161 ggtcaattcc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
     5221 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
     5281 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     5341 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
     5401 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt
     5461 tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac
     5521 accgggaccg atccagcctg gggatccacc atgaaccggg gagtcccttt taggcacttg
     5581 cttctggtgc tgcaactggc gctcctccca gcagccactc agggaaagaa agtggtgctg
     5641 ggcaaaaaag gggatacagt ggaactgacc tgtacagctt cccagaagaa gagcatacaa
     5701 ttccactgga aaaactccaa ccagataaag attctgggaa atcagggctc cttcttaact
     5761 aaaggtccat ccaagctgaa tgatcgcgct gactcaagaa gaagcctttg ggaccaagga
     5821 aacttccccc tgatcatcaa gaatcttaag atagaagact cagatactta catctgtgaa
     5881 gtggaggacc agaaggagga ggtgcaattg ctagtgttcg gattgactgc caactctgac
     5941 acccacctgc ttcaggggca gagcctgacc ctgaccttgg agagcccccc tggtagtagc
     6001 ccctcagtgc aatgtaggag tccaaggggt aaaaacatac agggggggaa gaccctctcc
     6061 gtgtctcagc tggagctcca ggatagtggc acctggacat gcactgtctt gcagaaccag
     6121 aagaaggtgg agttcaaaat agacatcgtg gtgctagctt tccagaaggc ctccagcata
     6181 gtctataaga aagaggggga acaggtggag ttctccttcc cactcgcctt tacagttgaa
     6241 aagctgacgg gcagtggcga gctgtggtgg caggcggaga gggcttcctc ctccaagtct
     6301 tggatcacct ttgacctgaa gaacaaggaa gtgtctgtaa aacgggttac ccaggaccct
     6361 aagctccaga tgggcaagaa gctcccgctc cacctcaccc tgccccaggc cttgcctcag
     6421 tatgctggct ctggaaacct caccctggcc cttgaagcga aaacaggaaa gttgcatcag
     6481 gaagtgaacc tggtggtgat gagagccact cagctccaga aaaatttgac ctgtgaggtg
     6541 tggggaccca cctcccctaa gctgatgctg agcttgaaac tggagaacaa ggaggcaaag
     6601 gtctcgaagc gggagaaggc ggtgtgggtg ctgaaccctg aggcggggat gtggcagtgt
     6661 ctgctgagtg actcgggaca ggtcctgctg gaatccaaca tcaaggttct gcccacatgg
     6721 tcgaccccgg tgcagccaat ggccctgatt gtgctggggg gcgtcgccgg cctcctgctt
     6781 ttcattgggc taggcatctt cttctgtgtc aggtgccggc actgaaggcg acctgcggat
     6841 cccacgtcac tattgtatac tctatattat actctatgtt atactctgta atcctactca
     6901 ataaacgtgt cacgcctgtg aaaccgtact aagtctcccg tgtcttctta tcaccatcag
     6961 gtgacatcct cgcccaggct gtcaatcatg ccggtatcga ttccagtagc accggcccca
     7021 cgctgacaac ccactcttgc agcgttagca gcgcccctct taacaagccg acccccacca
     7081 gcgtcgcggt tactaacact cctctccccg acctgcagcc caagcttggc gtaatcatgg
     7141 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     7201 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     7261 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     7321 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     7381 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     7441 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     7501 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     7561 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     7621 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     7681 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     7741 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     7801 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     7861 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     7921 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     7981 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     8041 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     8101 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     8161 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     8221 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     8281 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     8341 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     8401 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     8461 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     8521 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     8581 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     8641 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     8701 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     8761 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     8821 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     8881 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     8941 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     9001 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     9061 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     9121 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     9181 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     9241 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
     9301 gaaac
//
