LOCUS       sequence-383204-        9008 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector to integrate mScarlet-I C-terminal into the human PER1 
            Locus to express it as a fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9008)
  AUTHORS   Gabriel CH, Del Olmo M, Rizki Widini A, Roshanbin R, Woyde J, Hamza 
            E, Gutu NN, Zehtabian A, Ewers H, Granada A, Herzel H, Kramer A
  TITLE     Circadian period is compensated for repressor protein turnover rates
            in single cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2024 Aug 20;121(34):e2404738121. doi: 
            10.1073/pnas.2404738121. Epub 2024 Aug 14.
  PUBMED    39141353
REFERENCE   2  (bases 1 to 9008)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2024-08-20"; volume: "121(34):e2404738121. 
            doi"; pages: " 10.1073/pnas.2404738121. Epub 2024 Aug 14"
COMMENT     Sequence Label: pDB2-hPER1-mScarlet-I-CD4-bla
FEATURES             Location/Qualifiers
     source          1..9008
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(81..98)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(252..840)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(332..351)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1011..1802,1803..1871))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1634..1653
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1872..1976)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2044..2062
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2100..2122)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2222..2241
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             3251..3943
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet-I
                     /note="matures faster than mScarlet"
                     /translation="VSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGGA
                     VTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIKM
                     ALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRHS
                     TGGMDELYK"
     primer_bind     complement(3381..3399)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3649..3668
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             3950..4015
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(4025..4058)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(4141..4161)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4146..4476
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4327..4462
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      4526..4535
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4532..4534,4535..4537,4538..5248)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4577..4598)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4838..4857)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5185..5206
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5264..5320
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5327..5722
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="AKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    5734..5855
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5771..5790)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5825..5844
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    5883..5916
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     protein_bind    complement(5917..5950)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        6971..7174
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7125..7145
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     7171..7195
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(7269..7343,7344..8540)
                     /codon_start=1
                     /product="truncated human CD4 cell surface glycoprotein 
                     lacking the cytoplasmic tail"
                     /label=CD4 (truncated)
                     /translation="MNRGVPFRHLLLVLQLALLPAATQGKKVVLGKKGDTVELTCTASQ
                     KKSIQFHWKNSNQIKILGNQGSFLTKGPSKLNDRADSRRSLWDQGNFPLIIKNLKIEDS
                     DTYICEVEDQKEEVQLLVFGLTANSDTHLLQGQSLTLTLESPPGSSPSVQCRSPRGKNI
                     QGGKTLSVSQLELQDSGTWTCTVLQNQKKVEFKIDIVVLAFQKASSIVYKKEGEQVEFS
                     FPLAFTVEKLTGSGELWWQAERASSSKSWITFDLKNKEVSVKRVTQDPKLQMGKKLPLH
                     LTLPQALPQYAGSGNLTLALEAKTGKLHQEVNLVVMRATQLQKNLTCEVWGPTSPKLML
                     SLKLENKEAKVSKREKAVWVLNPEAGMWQCLLSDSGQVLLESNIKVLPTWSTPVQPMAL
                     IVLGGVAGLLLFIGLGIFFCVRCRH"
     primer_bind     complement(8858..8874)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8858..8874)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8871..8893)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8882..8898
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8906..8912,8913..8930,8931..8936))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8951..8972
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
       61 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
      121 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
      181 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
      241 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
      301 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
      361 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
      421 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
      481 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
      541 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
      601 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
      661 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
      721 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
      781 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
      841 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
      901 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      961 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     1021 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     1081 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     1141 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     1201 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     1261 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     1321 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     1381 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     1441 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     1501 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     1561 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     1621 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     1681 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     1741 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     1801 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1861 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1921 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1981 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     2041 aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac
     2101 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     2161 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat
     2221 gcggcatcag agcagattgt actgagagtg caccatatgg gccggccctt aaggtttaaa
     2281 ctctgaaccc cttcttgggc agggacatga cctctgtgct gaagcaggat cgggagcggc
     2341 tccgagccat gcagaagcag cagcctcggt tttctgagga ccagcggcgg gaactgggtg
     2401 ctgtgcactc ctgggtccgg aagggccaac tgcctcgggc tcttgatgtg atggtgagag
     2461 aagcctggga cggggagaaa aaagaattga gctcaagttc aagggggaga aaaaagaatt
     2521 gagctcaagt tcaaggggga gaaaaaagaa ttgagctcaa gttcaaggga tcgaggccaa
     2581 gagctgatct ccttgatgtc cttggatcat taattctgaa gaatgttgat tccactaaat
     2641 ttgctgtgga ttatagaata ttaagccgcg tgagtctttg cagaactttt cacagcctat
     2701 cctatgctaa tatgcattgt gactgtcctg taacggcatc tgggtagagg gcacaaggca
     2761 ctgtccaacc ttgttggacc gcaggtgcat ctgtgtggac tggtgcttct tgggagtaca
     2821 tttcgggaag cacagtgggc tgggggtggg aagctgcgct ggcaggttag cagtgagaac
     2881 cctgtctgac tctctcatgt ccatttctct caccaaggcc tgtgtggact gtgggagcag
     2941 cacccaagat cctggtcacc ctgatgaccc actcttctca gagctggatg gactggggct
     3001 ggagcccatg gaagagggtg gaggcgagca gggcagcagc ggtggcggca gtggtgaggg
     3061 agagggctgc gaggaggccc aaggcggggc caaggcttca agctctcagg acttggctat
     3121 ggaggaggag gaagaaggca ggagctcatc cagtccagcc ttacctacag caggaaactg
     3181 caccagcacc ggtggcggtg gtagcggcgg tggtggctct ggtggcggtg gttcaacgcg
     3241 tggtggcagc gtgagcaagg gcgaggcagt gatcaaggag ttcatgcggt tcaaggtgca
     3301 catggagggc tccatgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
     3361 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttctc
     3421 ctgggacatc ctgtcccctc agttcatgta cggctccagg gccttcatca agcaccccgc
     3481 cgacatcccc gactactata agcagtcctt ccccgagggc ttcaagtggg agcgcgtgat
     3541 gaacttcgag gacggcggcg ccgtgaccgt gacccaggac acctccctgg aggacggcac
     3601 cctgatctac aaggtgaagc tccgcggcac caacttccct cctgacggcc ccgtaatgca
     3661 gaagaagaca atgggctggg aagcgtccac cgagcggttg taccccgagg acggcgtgct
     3721 gaagggcgac attaagatgg ccctgcgcct gaaggacggc ggccgctacc tggcggactt
     3781 caagaccacc tacaaggcca agaagcccgt gcagatgccc ggcgcctaca acgtcgaccg
     3841 caagttggac atcacctccc acaacgagga ctacaccgtg gtggaacagt acgaacgctc
     3901 cgagggccgc cactccaccg gcggcatgga cgagctgtac aagactagtg actacaaaga
     3961 ccatgacggt gattataaag atcatgacat cgattacaag gatgacgatg acaagttaga
     4021 tctaataact tcgtatagca tacattatac gaagttattt tgaataaaat atctttattt
     4081 tcattacatc tgtgtgttgg ttttttgtgt tgagctgatt taacaaaaat ttaacgctgt
     4141 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccaggcagg cagaagtatg
     4201 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     4261 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     4321 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     4381 atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag
     4441 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tattgtatat ccattttcgg
     4501 atctgatcag cactgagcag gtaccgccac catggtgagc aagggcgagg agctgttcac
     4561 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     4621 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
     4681 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct ggggcgtgca
     4741 gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     4801 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     4861 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     4921 cttcaaggag gacggcaaca tcctggggca caagctggag tacaacgcca tcagcgacaa
     4981 cgtctatatc accgccgaca agcagaagaa cggcatcaag gccaacttca agatccgcca
     5041 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     5101 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtcca agctgagcaa
     5161 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     5221 cactctcggc atggacgagc tgtacaagga attcggaagc ggagctacta acttcagcct
     5281 gctgaagcag gctggagacg tggaggagaa ccctggacct cacgtggcca agcctttgtc
     5341 tcaagaagaa tccaccctca ttgaaagagc aacggctaca atcaacagca tccccatctc
     5401 tgaagactac agcgtcgcca gcgcagctct ctctagcgac ggccgcatct tcactggtgt
     5461 caatgtatat cattttactg ggggaccttg tgcagaactc gtggtgctgg gcactgctgc
     5521 tgctgcggca gctggcaacc tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat
     5581 cttgagcccc tgcggacggt gccgacaggt gcttctcgat ctgcatcctg ggatcaaagc
     5641 catagtgaag gacagtgatg gacagccgac ggcagttggg attcgtgaat tgctgccctc
     5701 tggttatgtg tgggagggct aactcgagac cccaacttgt ttattgcagc ttataatggt
     5761 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     5821 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtataga agttcctatt
     5881 ccgaagttcc tattcttcaa atagtatagg aacttcataa cttcgtatag catacattat
     5941 acgaagttat ttgattaatt aagcgatcgc actccattct gggaccatct cgaggagtcg
     6001 atgagaggct gtcttctcct atgtcccaat tctcagaact cagatgtggc tagaccaacc
     6061 agtgggacac tgccccagct tctcccacca tagggggccg gacccccatc accagcctag
     6121 gatccagggg ctgcctctgg cctcttaggg agcagagagc agaactccgc agcccagccc
     6181 agaggagtgt cacctcccac ctttggagag gaatccttcc ctcccctgga caaagttgct
     6241 gacaagctgc tgaagtggcc tctccatatt ccagctgagc ctgaatctga ctcttgaggg
     6301 ttggggctgc acttatttat tgcggggaga cagctctctc tcccacctcc tccccagatg
     6361 ggaggagagc ctgaggccca agcaggaccc gggggttcca gcccctagct gctctggagt
     6421 gggggaggtt ggtggaccat ggagtccctg gtgctgcccc tcaggtggga cccaggcgtt
     6481 ctcagctgta ccctctgccg atggcatttg tgtttttgat atttgtgtct gttactactt
     6541 ttttaataca aaaagataaa aacgcccagg actttgtgga atgaagtttg gggttggggt
     6601 gaggggagca tgtcaagggg agatgtggcc ctgcttttgg gaaggttgta gtgagggtgt
     6661 acagggccat ctcctgacga cccccattcc tcttttcccc catcctgtcc aggtgagggc
     6721 cctgctgagc ccgctacgtc cacgtccagc tcagactctg ctgtcccctc tagcatggcc
     6781 tcagctgggt ttgccctggg aaggagagct gagaggtgcg tgtggccaag ggcttgggta
     6841 agctccagcc cgggtcacgg gtggccaagg cacctgcagg tcaattccct ggcattatgc
     6901 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     6961 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
     7021 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
     7081 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
     7141 gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg
     7201 gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctggg
     7261 gatccaccat gaaccgggga gtccctttta ggcacttgct tctggtgctg caactggcgc
     7321 tcctcccagc agccactcag ggaaagaaag tggtgctggg caaaaaaggg gatacagtgg
     7381 aactgacctg tacagcttcc cagaagaaga gcatacaatt ccactggaaa aactccaacc
     7441 agataaagat tctgggaaat cagggctcct tcttaactaa aggtccatcc aagctgaatg
     7501 atcgcgctga ctcaagaaga agcctttggg accaaggaaa cttccccctg atcatcaaga
     7561 atcttaagat agaagactca gatacttaca tctgtgaagt ggaggaccag aaggaggagg
     7621 tgcaattgct agtgttcgga ttgactgcca actctgacac ccacctgctt caggggcaga
     7681 gcctgaccct gaccttggag agcccccctg gtagtagccc ctcagtgcaa tgtaggagtc
     7741 caaggggtaa aaacatacag ggggggaaga ccctctccgt gtctcagctg gagctccagg
     7801 atagtggcac ctggacatgc actgtcttgc agaaccagaa gaaggtggag ttcaaaatag
     7861 acatcgtggt gctagctttc cagaaggcct ccagcatagt ctataagaaa gagggggaac
     7921 aggtggagtt ctccttccca ctcgccttta cagttgaaaa gctgacgggc agtggcgagc
     7981 tgtggtggca ggcggagagg gcttcctcct ccaagtcttg gatcaccttt gacctgaaga
     8041 acaaggaagt gtctgtaaaa cgggttaccc aggaccctaa gctccagatg ggcaagaagc
     8101 tcccgctcca cctcaccctg ccccaggcct tgcctcagta tgctggctct ggaaacctca
     8161 ccctggccct tgaagcgaaa acaggaaagt tgcatcagga agtgaacctg gtggtgatga
     8221 gagccactca gctccagaaa aatttgacct gtgaggtgtg gggacccacc tcccctaagc
     8281 tgatgctgag cttgaaactg gagaacaagg aggcaaaggt ctcgaagcgg gagaaggcgg
     8341 tgtgggtgct gaaccctgag gcggggatgt ggcagtgtct gctgagtgac tcgggacagg
     8401 tcctgctgga atccaacatc aaggttctgc ccacatggtc gaccccggtg cagccaatgg
     8461 ccctgattgt gctggggggc gtcgccggcc tcctgctttt cattgggcta ggcatcttct
     8521 tctgtgtcag gtgccggcac tgaaggcgac ctgcggatcc cacgtcacta ttgtatactc
     8581 tatattatac tctatgttat actctgtaat cctactcaat aaacgtgtca cgcctgtgaa
     8641 accgtactaa gtctcccgtg tcttcttatc accatcaggt gacatcctcg cccaggctgt
     8701 caatcatgcc ggtatcgatt ccagtagcac cggccccacg ctgacaaccc actcttgcag
     8761 cgttagcagc gcccctctta acaagccgac ccccaccagc gtcgcggtta ctaacactcc
     8821 tctccccgac ctgcagccca agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     8881 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     8941 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     9001 agtcggga
//
