LOCUS       sequence-38321-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Weinberg DE, Nakanishi K, Patel DJ, Bartel DP
  TITLE     The inside-out mechanism of dicers from budding yeasts.
  JOURNAL   Cell. 2011 Jul 22;146(2):262-76.
  PUBMED    21784247
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2011-07-22"; volume: "146(2)"; pages: "262-76"
COMMENT     Sequence Label: pRSF-KpDcr1deltaC(E147Q/D151N)
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             70..87
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             100..393
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="MSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPL
                     RRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIGG"
     CDS             512..523
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 ttttgtttaa ctttaataag gagatatacc atgggcagca gccatcatca tcatcatcac
       61 agcagcggcc tggtgccgcg cggcagccac atggcaagca tgtcggactc agaagtcaat
      121 caagaagcta agccagaggt caagccagaa gtcaagcctg agactcacat caatttaaag
      181 gtgtccgatg gatcttcaga gatcttcttc aagatcaaaa agaccactcc tttaagaagg
      241 ctgatggaag cgttcgctaa aagacagggt aaggaaatgg actccttaag attcttgtac
      301 gacggtatta gaatccaagc tgatcagacc cctgaagatt tggacatgga ggataacgat
      361 attattgagg ctcacagaga acagattggt ggatccaacg agttaaaagt tagagaattt
      421 tatagactcc acaatgcttg tgtgaaattg aaagagtcta ttaaattgat ttatgaaaac
      481 ccattggtaa ctgaccaaaa tgttttgaat ttgggaactg cagaaaatac tattgattat
      541 acaattttaa acacacctac actaaatgtt gctaaaactt tactgggcaa tagatattca
      601 ttagatctaa tagatctttt tcaatctcat gatttcaaag attcaaacac tgatgtggat
      661 atgtttatta aatatcctgt ggtttatgat gaaaatttgg aaaatttagc atttatgcat
      721 aaaagttttc caaatatgaa tgcacatcta aatgatgctc aaaagactca attatctaat
      781 gaaaggttac aatttttggg taatagttgg ctaggtgctt tagtttcata tattgtatac
      841 accagatttc cttctgcaaa
//
