LOCUS       sequence-383218-        9110 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector to integrate mScarlet-I C-terminal into the human CRY2 
            Locus to express it as a fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9110)
  AUTHORS   Gabriel CH, Del Olmo M, Rizki Widini A, Roshanbin R, Woyde J, Hamza 
            E, Gutu NN, Zehtabian A, Ewers H, Granada A, Herzel H, Kramer A
  TITLE     Circadian period is compensated for repressor protein turnover rates
            in single cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2024 Aug 20;121(34):e2404738121. doi: 
            10.1073/pnas.2404738121. Epub 2024 Aug 14.
  PUBMED    39141353
REFERENCE   2  (bases 1 to 9110)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2024-08-20"; volume: "121(34):e2404738121. 
            doi"; pages: " 10.1073/pnas.2404738121. Epub 2024 Aug 14"
COMMENT     Sequence Label: pDB2-hCRY2-mScarlet-I-CD4-bla
FEATURES             Location/Qualifiers
     source          1..9110
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             1185..1877
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet-I
                     /note="matures faster than mScarlet"
                     /translation="VSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGGA
                     VTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIKM
                     ALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRHS
                     TGGMDELYK"
     primer_bind     complement(1315..1333)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1583..1602
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             1884..1949
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(1959..1992)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(2075..2095)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2080..2410
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2261..2396
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2323..2342
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     regulatory      2460..2469
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2466..2468,2469..2471,2472..3182)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2511..2532)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2772..2791)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3119..3140
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3198..3254
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3261..3656
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="AKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    3668..3789
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3705..3724)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3759..3778
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    3817..3850
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     protein_bind    complement(3851..3884)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        5002..5205
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5156..5176
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5202..5226
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(5300..5374,5375..6571)
                     /codon_start=1
                     /product="truncated human CD4 cell surface glycoprotein 
                     lacking the cytoplasmic tail"
                     /label=CD4 (truncated)
                     /translation="MNRGVPFRHLLLVLQLALLPAATQGKKVVLGKKGDTVELTCTASQ
                     KKSIQFHWKNSNQIKILGNQGSFLTKGPSKLNDRADSRRSLWDQGNFPLIIKNLKIEDS
                     DTYICEVEDQKEEVQLLVFGLTANSDTHLLQGQSLTLTLESPPGSSPSVQCRSPRGKNI
                     QGGKTLSVSQLELQDSGTWTCTVLQNQKKVEFKIDIVVLAFQKASSIVYKKEGEQVEFS
                     FPLAFTVEKLTGSGELWWQAERASSSKSWITFDLKNKEVSVKRVTQDPKLQMGKKLPLH
                     LTLPQALPQYAGSGNLTLALEAKTGKLHQEVNLVVMRATQLQKNLTCEVWGPTSPKLML
                     SLKLENKEAKVSKREKAVWVLNPEAGMWQCLLSDSGQVLLESNIKVLPTWSTPVQPMAL
                     IVLGGVAGLLLFIGLGIFFCVRCRH"
     primer_bind     complement(6889..6905)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6889..6905)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6902..6924)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6913..6929
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6937..6943,6944..6961,6962..6967))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6982..7003
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7120..7137)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7291..7879)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7371..7390)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8050..8841,8842..8910))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8673..8692
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8911..9015)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9083..9101
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatggg ccggccctta aggtttaaac aggaaccgaa gggaggtcag tgtagcggca
      241 tgtcccagtg aggagctgga atgagatgag gacaagatac agcatgagca gcatgtggca
      301 ggcctgatgt ccgagacgag gactaggatt cagccttcag tgcactgaag gatggtcagt
      361 agggcagggc atgattggac ttaggtctga aaaagctccc tggcctgctg ggtggagatt
      421 ggacagtgta ggccggagcg ggcacaggga ggccagagag gaggcagcac agacagggtg
      481 gtggccgcaa ggagaaagac tggacagatt cgctgcattc ggagcctctt cccttcttgg
      541 ggatgaagat gagtaacatg agatgggaag aacacgggtt tttctagttg gcccagctag
      601 gctgcctcca tgggcagcat gactcctcca caggggccct gctctttgct gcttggggag
      661 gctgattcag ggttgcagag ggaatgcctc accttcaaag caggacagca ggcaggccaa
      721 atcagtctct ggccacagcc catgccattt ggggcccaaa agctggtcct gctaccagca
      781 ggcctgcttg gtcatgtcct ccttggacat ttccaggcaa agtcactgct gaaagcaagg
      841 ccgaggggca ggtctgagga gcagcatgct ggcattccat tgataagtgt gctccctgtc
      901 ccctaaagag ctgctgctgc atagtgtggg atcattttgg ctgcatgcac agtctgtgtg
      961 accctttgac acgcttccct acaggcccaa gaccactacc cagtggccca gcatccccca
     1021 aacgcaagct ggaagcagcc gaggaaccac ctggtgaaga actcagcaaa cgggcccggg
     1081 tggcagagtt gccaacccca gagctgccga gcaaggatgc caccggtggc ggtggtagcg
     1141 gcggtggtgg ctctggtggc ggtggttcaa cgcgtggtgg cagcgtgagc aagggcgagg
     1201 cagtgatcaa ggagttcatg cggttcaagg tgcacatgga gggctccatg aacggccacg
     1261 agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc
     1321 tgaaggtgac caagggtggc cccctgccct tctcctggga catcctgtcc cctcagttca
     1381 tgtacggctc cagggccttc atcaagcacc ccgccgacat ccccgactac tataagcagt
     1441 ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgccgtga
     1501 ccgtgaccca ggacacctcc ctggaggacg gcaccctgat ctacaaggtg aagctccgcg
     1561 gcaccaactt ccctcctgac ggccccgtaa tgcagaagaa gacaatgggc tgggaagcgt
     1621 ccaccgagcg gttgtacccc gaggacggcg tgctgaaggg cgacattaag atggccctgc
     1681 gcctgaagga cggcggccgc tacctggcgg acttcaagac cacctacaag gccaagaagc
     1741 ccgtgcagat gcccggcgcc tacaacgtcg accgcaagtt ggacatcacc tcccacaacg
     1801 aggactacac cgtggtggaa cagtacgaac gctccgaggg ccgccactcc accggcggca
     1861 tggacgagct gtacaagact agtgactaca aagaccatga cggtgattat aaagatcatg
     1921 acatcgatta caaggatgac gatgacaagt tagatctaat aacttcgtat agcatacatt
     1981 atacgaagtt attttgaata aaatatcttt attttcatta catctgtgtg ttggtttttt
     2041 gtgttgagct gatttaacaa aaatttaacg ctgtggaatg tgtgtcagtt agggtgtgga
     2101 aagtccccag gctccccagg caggcagaag tatgcaaagc atgcatctca attagtcagc
     2161 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     2221 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     2281 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     2341 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     2401 cttttgcaaa aagctattgt atatccattt tcggatctga tcagcactga gcaggtaccg
     2461 ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
     2521 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
     2581 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
     2641 ccaccctcgt gaccaccctg acctggggcg tgcagtgctt cgcccgctac cccgaccaca
     2701 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
     2761 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     2821 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     2881 ggcacaagct ggagtacaac gccatcagcg acaacgtcta tatcaccgcc gacaagcaga
     2941 agaacggcat caaggccaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc
     3001 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     3061 accactacct gagcacccag tccaagctga gcaaagaccc caacgagaag cgcgatcaca
     3121 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
     3181 aggaattcgg aagcggagct actaacttca gcctgctgaa gcaggctgga gacgtggagg
     3241 agaaccctgg acctcacgtg gccaagcctt tgtctcaaga agaatccacc ctcattgaaa
     3301 gagcaacggc tacaatcaac agcatcccca tctctgaaga ctacagcgtc gccagcgcag
     3361 ctctctctag cgacggccgc atcttcactg gtgtcaatgt atatcatttt actgggggac
     3421 cttgtgcaga actcgtggtg ctgggcactg ctgctgctgc ggcagctggc aacctgactt
     3481 gtatcgtcgc gatcggaaat gagaacaggg gcatcttgag cccctgcgga cggtgccgac
     3541 aggtgcttct cgatctgcat cctgggatca aagccatagt gaaggacagt gatggacagc
     3601 cgacggcagt tgggattcgt gaattgctgc cctctggtta tgtgtgggag ggctaactcg
     3661 agaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     3721 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     3781 tgtatcttat catgtctgta tagaagttcc tattccgaag ttcctattct tcaaatagta
     3841 taggaacttc ataacttcgt atagcataca ttatacgaag ttatttgatt aattaagcga
     3901 tcgcggatgc ctgagagtga gtgacagcag cctagattca acctcacgaa cgaagttggg
     3961 agtggggggg cctactgccc tgccagctgc aggttgacac atagcacact agatgaaaat
     4021 ctggtgggac cacccaatgc tcagccacta aagctttacc attttacagt ggcagccggg
     4081 ttccatcctg gcttagggaa tgagtacttt ctggttgata tctgtgtgac ctttaccatt
     4141 gggccggcta tggtggctca tgtatgtaat cccagcactt tgggaggcca aggtgggagg
     4201 atcgcttgag gccaggagta caagaccaac ctggccaaac caacatagtg agactccatc
     4261 tctaaaaaca taataataat aataataata atctggtggg actgcatgac ttttaggatt
     4321 tcagctcaaa aatcccacca ttctgacttt tatcagagat ttaaaagtca agcccttttg
     4381 tatttttttg ccagctaagg gaaaaaggga ggcatggggg ctgaggggtg gcgctttcta
     4441 cctattaaaa tgagtcacac tcaagagaaa gaaagctgag acctgcgtgt gaagcagcgg
     4501 ggaaaaggcg cattgcaggg tctgaggctg ctccaggccc cctgctttcc gacaggctgt
     4561 gttctatagg ccagccagtg gagggcagca ctgaccttga gaagcctgga cagagggctg
     4621 tgctgtgtct tacttggccc agccctgcct gaggaccagt agacccagtc tgtcatcatc
     4681 tttgcctttt gcggcctctc ctcccttctc acccatcctg tggcctcagc tctgcacctc
     4741 cctggcagcg ctcatcttgg aagcctcctg aatgtcatca aaacactggc ttctcctttc
     4801 ccaagggagg aagggccttt tatgccactc ttggggaaat gtaaattcct gtcccctctg
     4861 agtcatcatc tcaagctgtt ccttatgcac catgtcttcg tcacctgcag gtcaattccc
     4921 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
     4981 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
     5041 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
     5101 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
     5161 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcgttt agtgaaccgt
     5221 cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgggaccga
     5281 tccagcctgg ggatccacca tgaaccgggg agtccctttt aggcacttgc ttctggtgct
     5341 gcaactggcg ctcctcccag cagccactca gggaaagaaa gtggtgctgg gcaaaaaagg
     5401 ggatacagtg gaactgacct gtacagcttc ccagaagaag agcatacaat tccactggaa
     5461 aaactccaac cagataaaga ttctgggaaa tcagggctcc ttcttaacta aaggtccatc
     5521 caagctgaat gatcgcgctg actcaagaag aagcctttgg gaccaaggaa acttccccct
     5581 gatcatcaag aatcttaaga tagaagactc agatacttac atctgtgaag tggaggacca
     5641 gaaggaggag gtgcaattgc tagtgttcgg attgactgcc aactctgaca cccacctgct
     5701 tcaggggcag agcctgaccc tgaccttgga gagcccccct ggtagtagcc cctcagtgca
     5761 atgtaggagt ccaaggggta aaaacataca gggggggaag accctctccg tgtctcagct
     5821 ggagctccag gatagtggca cctggacatg cactgtcttg cagaaccaga agaaggtgga
     5881 gttcaaaata gacatcgtgg tgctagcttt ccagaaggcc tccagcatag tctataagaa
     5941 agagggggaa caggtggagt tctccttccc actcgccttt acagttgaaa agctgacggg
     6001 cagtggcgag ctgtggtggc aggcggagag ggcttcctcc tccaagtctt ggatcacctt
     6061 tgacctgaag aacaaggaag tgtctgtaaa acgggttacc caggacccta agctccagat
     6121 gggcaagaag ctcccgctcc acctcaccct gccccaggcc ttgcctcagt atgctggctc
     6181 tggaaacctc accctggccc ttgaagcgaa aacaggaaag ttgcatcagg aagtgaacct
     6241 ggtggtgatg agagccactc agctccagaa aaatttgacc tgtgaggtgt ggggacccac
     6301 ctcccctaag ctgatgctga gcttgaaact ggagaacaag gaggcaaagg tctcgaagcg
     6361 ggagaaggcg gtgtgggtgc tgaaccctga ggcggggatg tggcagtgtc tgctgagtga
     6421 ctcgggacag gtcctgctgg aatccaacat caaggttctg cccacatggt cgaccccggt
     6481 gcagccaatg gccctgattg tgctgggggg cgtcgccggc ctcctgcttt tcattgggct
     6541 aggcatcttc ttctgtgtca ggtgccggca ctgaaggcga cctgcggatc ccacgtcact
     6601 attgtatact ctatattata ctctatgtta tactctgtaa tcctactcaa taaacgtgtc
     6661 acgcctgtga aaccgtacta agtctcccgt gtcttcttat caccatcagg tgacatcctc
     6721 gcccaggctg tcaatcatgc cggtatcgat tccagtagca ccggccccac gctgacaacc
     6781 cactcttgca gcgttagcag cgcccctctt aacaagccga cccccaccag cgtcgcggtt
     6841 actaacactc ctctccccga cctgcagccc aagcttggcg taatcatggt catagctgtt
     6901 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     6961 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     7021 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     7081 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     7141 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     7201 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     7261 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     7321 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     7381 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     7441 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     7501 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     7561 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     7621 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     7681 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     7741 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     7801 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     7861 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     7921 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     7981 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     8041 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     8101 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     8161 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     8221 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     8281 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     8341 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     8401 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     8461 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     8521 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     8581 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     8641 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     8701 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     8761 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     8821 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     8881 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     8941 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     9001 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
     9061 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
//
