LOCUS       sequence-383220-        7340 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cre-dependent expression of a green fluorescent, negative 
            response-polarity voltage indicator; soma-targeted.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7340)
  AUTHORS   Kannan M, Vasan G, Haziza S, Huang C, Chrapkiewicz R, Luo J, Cardin 
            JA, Schnitzer MJ, Pieribone VA
  TITLE     Dual-polarity voltage imaging of the concurrent dynamics of multiple
            neuron types.
  JOURNAL   Science. 2022 Nov 4;378(6619):eabm8797. doi: 
            10.1126/science.abm8797. Epub 2022 Nov 4.
  PUBMED    36378956
REFERENCE   2  (bases 1 to 7340)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-11-4"; volume: "378(6619):eabm8797. doi"; pages: " 
            10.1126/science.abm8797. Epub 2022 Nov 4"
COMMENT     Sequence Label: pAAV-CAG-DIO-Ace-mNeon2-Kv2.1PR
FEATURES             Location/Qualifiers
     source          1..7340
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     enhancer        134..513
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        515..792
                     /label=chicken beta-actin promoter
     intron          793..1809
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1733..1755
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1817..1836
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    complement(1876..1909)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(1960..1993)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      2722..2731
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      3631..3640
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    3646..3679
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    3730..3763
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    3781..4369
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3834..3854)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4252..4263)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4377..4601
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   4603..4743
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4603..4732
                     /label=AAV2 ITR
     rep_origin      4818..5273
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4905..4924)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5115..5136
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5290..5309)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5409..5431
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5469..5487)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5555..5659
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5660..5728,5729..6520)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5878..5897)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6691..7279
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7180..7199
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gtcgacattg attattgact agttattaat agtaatcaat tacggggtca
      181 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      241 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
      301 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      361 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      421 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      481 tacatctacg tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc
      541 actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta
      601 ttttgtgcag cgatgggggc gggggggggg ggggggcgcg cgccaggcgg ggcggggcgg
      661 ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg
      721 ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc
      781 gcgcggcggg cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc cgccgccgcc
      841 tcgcgccgcc cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac
      901 ggcccttctc ctccgggctg taattagcgc ttggtttaat gacggcttgt ttcttttctg
      961 tggctgcgtg aaagccttga ggggctccgg gagggccctt tgtgcggggg gagcggctcg
     1021 gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc
     1081 ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg
     1141 agcgcggccg ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa caaaggctgc
     1201 gtgcggggtg tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa
     1261 ccccccctgc acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc
     1321 cgtacggggc gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg
     1381 ccgggcgggg cggggccgcc tcgggccggg gagggctcgg gggaggggcg cggcggcccc
     1441 cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg
     1501 tgcgagaggg cgcagggact tcctttgtcc caaatctgtg cggagccgaa atctgggagg
     1561 cgccgccgca ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa
     1621 atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc cccttctccc tctccagcct
     1681 cggggctgtc cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc
     1741 ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt
     1801 ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa
     1861 ttcgatgact ccggaataac ttcgtatagg atactttata cgaagttatg cagaatggta
     1921 gctggattgt agctgctatt agcaatatga aacctcttaa taacttcgta tagcatacat
     1981 tatacgaagt tatggcgcgt tagaatcgag tagcctcggg gaaatctgtt gcgcaagaga
     2041 tgaagctatc aatagatgac atactcctca tgtcaatcac tccttcagtc ctggcaggga
     2101 ggggagccac gggacttggc atactggaca tctcaagctc ttcgggaggc ttagactgtg
     2161 gagccatttc ctttgtgttc aggataggct gactttgcac ctcgttctcg tagcagaaca
     2221 cgttgatgtc gatctggtcc agggggatgt actcgccctc gctggtgatc ctgctcttct
     2281 tgtacagctc gtccatgccc atcacatcgg taaaggcctt ttgccactcc ttgaagttga
     2341 gctcggtctt ggagtgcttg agctccgtct tacggaacac gtacatcggc tggttcttca
     2401 gatagttagc cgccattggc ttggcaaagg tgtaggtggt ccgcgcagtg ctcctgtagc
     2461 gcttgccatt tccagtggtg taactccact taaaggtact gatgatggtt ttgtcgttgg
     2521 ggtaagtctt cttcgacctg caccagtccg cagcggtcag cgagttggtc atcacaggac
     2581 cgtcagcagg gaaaccagtc cccttcacct gggcctctcc tttgatgtgg cttccctcgt
     2641 aggtgtagcg gtagttaaca gtaagggagg caccatcttc aaactgcatt gtgcgatgga
     2701 cttggtatcc ggagccatct accatggcgg cctggaaagg cgacatcccg tcagggtagg
     2761 gcaggtactg atggaagcca tacccgatat gagggaccag aatccagggg gagaactgga
     2821 ggtcaccctt ggtggacttc aggtttaact cctcataacc atcatttgga ttgccggtgc
     2881 cctgacccac catgtcaaag tccacaccgt tgatggagcc aaagatgtgt aactcatgtg
     2941 tcgctgggta ggacttgaag atagtctcat gggcaatgag gcaccccatt ccaaacagtg
     3001 gcttagcgag cacatccagg attcccatga gaatggcctc attgtcgacg gacagcacgc
     3061 cactaccaga cccgaaagcc cacacgacag gataaaacac ccagaggatt gacaggaaga
     3121 cgagcagact gacataggag ctatggtact cggggctttt cttcagatcg tctttccaag
     3181 tggcgttata catcccgtat gtcaggactg cctgcatcac acatccagcg ataaagtaca
     3241 cccatttgaa cttgtgttca tcctcgaagg cgcccagaat accaaacacg atcatgaaaa
     3301 tgtctgcgcc gatgacccaa gaaatcatca ctccgcccat cttggtcatg acgatgagat
     3361 cgagcaggag cagtggtgtg gtcagcaccc aggaaatata tcttgcgtag accacctgcc
     3421 tttcaccatt cattgtcagg tcctggtagt tcacggccat tgcatagtaa gcggtagcgg
     3481 cgatagtgca gatggcaatg ttgatatagt atgcgctctt cagctctgaa ggccgaaagt
     3541 gcacagccac tccaaaagca atagcagcag cagccataat agcaaagaca atccactgtg
     3601 caatcatgcc ggtctcggtt tccacgtcag ccatggtggc ctagcataac ttcgtataaa
     3661 gtatcctata cgaagttatt tgccttaacc cagaaattat cactgttatt ctttagaatg
     3721 gtgcaaagaa taacttcgta taatgtatgc tatacgaagt tatgaattcg atatctccgg
     3781 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     3841 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     3901 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     3961 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     4021 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     4081 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     4141 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc
     4201 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     4261 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     4321 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggatcctgt
     4381 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     4441 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     4501 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     4561 agacaatagc aggcatgctg gggatgcggt gggctctatg gcaggaaccc ctagtgatgg
     4621 agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg
     4681 cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc
     4741 aggggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac
     4801 gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     4861 tacgcgcagc gtgaccgcta cacttgccag cgccttagcg cccgctcctt tcgctttctt
     4921 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     4981 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga
     5041 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     5101 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaact ctatctcggg
     5161 ctattctttt gatttataag ggattttgcc gatttcggtc tattggttaa aaaatgagct
     5221 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa ttttatggtg
     5281 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
     5341 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     5401 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
     5461 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     5521 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     5581 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     5641 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     5701 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     5761 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     5821 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     5881 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     5941 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     6001 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     6061 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     6121 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     6181 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     6241 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     6301 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     6361 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     6421 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     6481 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     6541 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     6601 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     6661 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     6721 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     6781 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     6841 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     6901 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     6961 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     7021 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     7081 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     7141 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     7201 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     7261 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     7321 ctggcctttt gctcacatgt
//
