LOCUS       sequence-383241-        7364 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cre-dependent red fluorescent, positive response-polarity voltage 
            indicator; soma-targeted.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7364)
  AUTHORS   Kannan M, Vasan G, Haziza S, Huang C, Chrapkiewicz R, Luo J, Cardin 
            JA, Schnitzer MJ, Pieribone VA
  TITLE     Dual-polarity voltage imaging of the concurrent dynamics of multiple
            neuron types.
  JOURNAL   Science. 2022 Nov 4;378(6619):eabm8797. doi: 
            10.1126/science.abm8797. Epub 2022 Nov 4.
  PUBMED    36378956
REFERENCE   2  (bases 1 to 7364)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-11-4"; volume: "378(6619):eabm8797. doi"; pages: " 
            10.1126/science.abm8797. Epub 2022 Nov 4"
COMMENT     Sequence Label: pAAV-CAG-DIO-pAceR-Kv2.1PR
FEATURES             Location/Qualifiers
     source          1..7364
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     enhancer        134..513
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        515..792
                     /label=chicken beta-actin promoter
     intron          793..1809
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1733..1755
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1817..1836
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    complement(1876..1909)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(1960..1993)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      3654..3663
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    3670..3703
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    3754..3787
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    3805..4393
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3858..3878)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4276..4287)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4401..4625
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   4627..4767
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4627..4756
                     /label=AAV2 ITR
     rep_origin      4842..5297
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4929..4948)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5139..5160
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5314..5333)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5433..5455
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5493..5511)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5579..5683
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5684..5752,5753..6544)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5902..5921)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6715..7303
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7204..7223
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gtcgacattg attattgact agttattaat agtaatcaat tacggggtca
      181 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      241 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
      301 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      361 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      421 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      481 tacatctacg tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc
      541 actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta
      601 ttttgtgcag cgatgggggc gggggggggg ggggggcgcg cgccaggcgg ggcggggcgg
      661 ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg
      721 ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc
      781 gcgcggcggg cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc cgccgccgcc
      841 tcgcgccgcc cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac
      901 ggcccttctc ctccgggctg taattagcgc ttggtttaat gacggcttgt ttcttttctg
      961 tggctgcgtg aaagccttga ggggctccgg gagggccctt tgtgcggggg gagcggctcg
     1021 gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc
     1081 ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg
     1141 agcgcggccg ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa caaaggctgc
     1201 gtgcggggtg tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa
     1261 ccccccctgc acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc
     1321 cgtacggggc gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg
     1381 ccgggcgggg cggggccgcc tcgggccggg gagggctcgg gggaggggcg cggcggcccc
     1441 cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg
     1501 tgcgagaggg cgcagggact tcctttgtcc caaatctgtg cggagccgaa atctgggagg
     1561 cgccgccgca ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa
     1621 atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc cccttctccc tctccagcct
     1681 cggggctgtc cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc
     1741 ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt
     1801 ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa
     1861 ttcgatgact ccggaataac ttcgtatagg atactttata cgaagttatg cagaatggta
     1921 gctggattgt agctgctatt agcaatatga aacctcttaa taacttcgta tagcatacat
     1981 tatacgaagt tatggcgcgc cttagaatcg agtagcctcg gggaaatctg ttgcgcaaga
     2041 gatgaagcta tcaatagatg acatactcct catgtcaatc actccttcag tcctggcagg
     2101 gaggggagcc acgggacttg gcatactgga catctcaagc tcttcgggag gcttagactg
     2161 tggagccatt tcctttgtgt tcaggatagg ctgactttgc acctcgttct cgtagcagaa
     2221 cacgttgatg tcgatctggt ccagggggat gtactcgccc tcgctggtga tcctgctctt
     2281 cttatacagc tcgtccatcc cgccgcccag attagagtac ttggccacgg ccacctctct
     2341 ctgcaccaca tatgtctcgt tatcagactc ctcgatgcgc tccagcctgt ggtccacggc
     2401 gtgcactcct ggcatcttga tattgcccac tgtcttcttg gagcggtatg tggtcacaaa
     2461 gttacagtgc aggtgtcctc ctccatccac cttcagggcg atgtcggtat atcccctcag
     2521 gcctccgtct gcagggtaca tcatctctgt attgggctcc cagcccttgg tcttcttctg
     2581 catcactggg ccattgctag ggaagttcac gcccctcacc ttcacgttat acaccagctc
     2641 gccatcctcc agagaggtgt cctgtgtcac ggtcaccacg ccgccatcct cgtatcttgt
     2701 caccctctcc caggtaaagc cctcggggaa gctctgctta aagaaatcgg ggatgtcggc
     2761 tggatacttg atgaaggtcc tgctgccgta catgaagctg gtggccagga tgtcaaaggc
     2821 gaaaggcagt ggtcctccct cgatcacttt gatcctcatt gtctgcacgc cctcgtatgg
     2881 cctgccctct ccctctccgg tgcacttgaa ctggtgccca ttgacagacc cttccatcac
     2941 gaccttcatc cgcatatttt ccttaatcag gatggacttg aagatagtct catgggcaat
     3001 gaggcacccc attccaaaca gtggcttagc gagcacatcc aggattccca tgagaatggc
     3061 ctcattgtcg acggacagca cgccactacc agacccgaaa gcccacacga caggataaaa
     3121 cacccagagg attgacagga agacgagcag actgacatag gagctatggt actcggggct
     3181 tttcttcaga tcgtctttcc aagtggcgtt atacatcccg tatgtcagga ctgcctgcat
     3241 cacacatcca gcgataaagt acacccattt gaacttgtgt tcatcctcga aggcgcccag
     3301 aataccaaac acgatcatga aaatgtctgc gccgatgacc caagaaatca tcactccgcc
     3361 catcttggtc atgacgatga ggttgagcag gagcagtggt gtggtcagca cccagtcaat
     3421 atactctgcg tagaccacct gcctttcacc attcattgtc aggtcctggt agttcacggc
     3481 cattgcatag taagcggtag cggcgatagt gcagatggca atgttgatat agtatgcgct
     3541 cttcagctct gaaggccgaa agtgcacagc cactccaaaa gcaatagcag cagcagccat
     3601 aatagcaaag acaatccact gtgcaatcat gccggtctcg gtttccacgt cagccatggt
     3661 ggcgctagca taacttcgta taaagtatcc tatacgaagt tatttgcctt aacccagaaa
     3721 ttatcactgt tattctttag aatggtgcaa agaataactt cgtataatgt atgctatacg
     3781 aagttatgaa ttcgatatct ccggaatcaa cctctggatt acaaaatttg tgaaagattg
     3841 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     3901 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     3961 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     4021 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     4081 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     4141 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag
     4201 ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     4261 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     4321 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     4381 gccgcctccc cgcctggatc ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     4441 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     4501 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     4561 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
     4621 tatggcagga acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca
     4681 ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga
     4741 gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc cttacgcatc
     4801 tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc
     4861 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgcctt
     4921 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     4981 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     5041 ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     5101 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     5161 aacaacactc aactctatct cgggctattc ttttgattta taagggattt tgccgatttc
     5221 ggtctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     5281 attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     5341 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     5401 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     5461 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
     5521 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     5581 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     5641 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     5701 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     5761 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     5821 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     5881 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     5941 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     6001 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     6061 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     6121 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     6181 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     6241 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     6301 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     6361 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     6421 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     6481 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     6541 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     6601 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     6661 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     6721 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     6781 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     6841 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     6901 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     6961 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     7021 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     7081 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     7141 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     7201 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     7261 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     7321 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgt
//
