LOCUS       sequence-383243-        6978 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Green fluorescent, negative response-polarity voltage indicator 
            under the control of CaMKII promoter; soma-targeted.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6978)
  AUTHORS   Kannan M, Vasan G, Haziza S, Huang C, Chrapkiewicz R, Luo J, Cardin 
            JA, Schnitzer MJ, Pieribone VA
  TITLE     Dual-polarity voltage imaging of the concurrent dynamics of multiple
            neuron types.
  JOURNAL   Science. 2022 Nov 4;378(6619):eabm8797. doi: 
            10.1126/science.abm8797. Epub 2022 Nov 4.
  PUBMED    36378956
REFERENCE   2  (bases 1 to 6978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-11-4"; volume: "378(6619):eabm8797. doi"; pages: " 
            10.1126/science.abm8797. Epub 2022 Nov 4"
COMMENT     Sequence Label: pAAV-CaMKII-Ace-mNeon2-Kv2.1PR
FEATURES             Location/Qualifiers
     source          1..6978
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        149..1437
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     regulatory      1452..1461
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2361..2370
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    3105..3693
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3158..3178)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3576..3587)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3725..4201
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3924..3943)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   4241..4381
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4241..4370
                     /label=AAV2 ITR
     rep_origin      4456..4911
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4543..4562)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4753..4774
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4928..4947)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5047..5069
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5107..5125)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5193..5297
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5298..5366,5367..6158)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5516..5535)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6329..6917
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6818..6837
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtttaaca ttatggcctt aggtcacttc atctccatgg
      181 ggttcttctt ctgattttct agaaaatgag atgggggtgc agagagcttc ctcagtgacc
      241 tgcccagggt cacatcagaa atgtcagagc tagaacttga actcagatta ctaatcttaa
      301 attccatgcc ttgggggcat gcaagtacga tatacagaag gagtgaactc attagggcag
      361 atgaccaatg agtttaggaa agaagagtcc agggcagggt acatctacac cacccgccca
      421 gccctgggtg agtccagcca cgttcacctc attatagttg cctctctcca gtcctacctt
      481 gacgggaagc acaagcagaa actgggacag gagccccagg agaccaaatc ttcatggtcc
      541 ctctgggagg atgggtgggg agagctgtgg cagaggcctc aggaggggcc ctgctgctca
      601 gtggtgacag ataggggtga gaaagcagac agagtcattc cgtcagcatt ctgggtctgt
      661 ttggtacttc ttctcacgct aaggtggcgg tgtgatatgc acaatggcta aaaagcaggg
      721 agagctggaa agaaacaagg acagagacag aggccaagtc aaccagacca attcccagag
      781 gaagcaaaga aaccattaca gagactacaa gggggaaggg aaggagagat gaattagctt
      841 cccctgtaaa ccttagaacc cagctgttgc cagggcaacg gggcaatacc tgtctcttca
      901 gaggagatga agttgccagg gtaactacat cctgtctttc tcaaggacca tcccagaatg
      961 tggcacccac tagccgttac catagcaact gcctctttgc cccacttaat cccatcccgt
     1021 ctgttaaaag ggccctatag ttggaggtgg gggaggtagg aagagcgatg atcacttgtg
     1081 gactaagttt gttcgcatcc ccttctccaa ccccctcagt acatcaccct gggggaacag
     1141 ggtccacttg ctcctgggcc cacacagtcc tgcagtattg tgtatataag gccagggcaa
     1201 agaggagcag gttttaaagt gaaaggcagg caggtgttgg ggaggcagtt accggggcaa
     1261 cgggaacagg gcgtttcgga ggtggttgcc atggggacct ggatgctgac gaaggctcgc
     1321 gaggctgtga gcagccacag tgccctgctc agaagcccca agctcgtcag tcaagccggt
     1381 tctccgtttg cactcaggag cacgggcagg cgagtggccc ctagttctgg gggcagctct
     1441 agagcggtac cgccaccatg gctgacgtgg aaaccgagac cggcatgatt gcacagtgga
     1501 ttgtctttgc tattatggct gctgctgcta ttgcttttgg agtggctgtg cactttcggc
     1561 cttcagagct gaagagcgca tactatatca acattgccat ctgcactatc gccgctaccg
     1621 cttactatgc aatggccgtg aactaccagg acctgacaat gaatggtgaa aggcaggtgg
     1681 tctacgcaag atatatttcc tgggtgctga ccacaccact gctcctgctc gatctcatcg
     1741 tcatgaccaa gatgggcgga gtgatgattt cttgggtcat cggcgcagac attttcatga
     1801 tcgtgtttgg tattctgggc gccttcgagg atgaacacaa gttcaaatgg gtgtacttta
     1861 tcgctggatg tgtgatgcag gcagtcctga catacgggat gtataacgcc acttggaaag
     1921 acgatctgaa gaaaagcccc gagtaccata gctcctatgt cagtctgctc gtcttcctgt
     1981 caatcctctg ggtgttttat cctgtcgtgt gggctttcgg gtctggtagt ggcgtgctgt
     2041 ccgtcgacaa tgaggccatt ctcatgggaa tcctggatgt gctcgctaag ccactgtttg
     2101 gaatggggtg cctcattgcc catgagacta tcttcaagtc ctacccagcg acacatgagt
     2161 tacacatctt tggctccatc aacggtgtgg actttgacat ggtgggtcag ggcaccggca
     2221 atccaaatga tggttatgag gagttaaacc tgaagtccac caagggtgac ctccagttct
     2281 ccccctggat tctggtccct catatcgggt atggcttcca tcagtacctg ccctaccctg
     2341 acgggatgtc gcctttccag gccgccatgg tagatggctc cggataccaa gtccatcgca
     2401 caatgcagtt tgaagatggt gcctccctta ctgttaacta ccgctacacc tacgagggaa
     2461 gccacatcaa aggagaggcc caggtgaagg ggactggttt ccctgctgac ggtcctgtga
     2521 tgaccaactc gctgaccgct gcggactggt gcaggtcgaa gaagacttac cccaacgaca
     2581 aaaccatcat cagtaccttt aagtggagtt acaccactgg aaatggcaag cgctacagga
     2641 gcactgcgcg gaccacctac acctttgcca agccaatggc ggctaactat ctgaagaacc
     2701 agccgatgta cgtgttccgt aagacggagc tcaagcactc caagaccgag ctcaacttca
     2761 aggagtggca aaaggccttt accgatgtga tgggcatgga cgagctgtac aagaagagca
     2821 ggatcaccag cgagggcgag tacatccccc tggaccagat cgacatcaac gtgttctgct
     2881 acgagaacga ggtgcaaagt cagcctatcc tgaacacaaa ggaaatggct ccacagtcta
     2941 agcctcccga agagcttgag atgtccagta tgccaagtcc cgtggctccc ctccctgcca
     3001 ggactgaagg agtgattgac atgaggagta tgtcatctat tgatagcttc atctcttgcg
     3061 caacagattt ccccgaggct actcgattct aaaagcttat cgataatcaa cctctggatt
     3121 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     3181 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     3241 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     3301 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     3361 ccacctgtca gctcctttcc ggaactttcg ctttccccct ccctattgcc acggcggaac
     3421 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     3481 ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctat gttgccacct
     3541 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     3601 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     3661 cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgagcgctg ctcgagagat
     3721 ctacgggtgg catccctgtg acccctcccc agtgcctctc ctggccctgg aagttgccac
     3781 tccagtgccc accagccttg tcctaataaa attaagttgc atcattttgt ctgactaggt
     3841 gtccttctat aatattatgg ggtggagggg ggtggtatgg agcaaggggc aagttgggaa
     3901 gacaacctgt agggcctgcg gggtctattg ggaaccaagc tggagtgcag tggcacaatc
     3961 ttggctcact gcaatctccg cctcctgggt tcaagcgatt ctcctgcctc agcctcccga
     4021 gttgttggga ttccaggcat gcatgaccag gctcagctaa tttttgtttt tttggtagag
     4081 acggggtttc accatattgg ccaggctggt ctccaactcc taatctcagg tgatctaccc
     4141 accttggcct cccaaattgc tgggattaca ggcgtgaacc actgctccct tccctgtcct
     4201 tctgattttg taggtaacca cgtgcggacc gagcggccgc aggaacccct agtgatggag
     4261 ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc
     4321 cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag
     4381 gggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgt
     4441 caaagcaacc atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     4501 cgcgcagcgt gaccgctaca cttgccagcg ccttagcgcc cgctcctttc gctttcttcc
     4561 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     4621 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg
     4681 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     4741 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaactct atctcgggct
     4801 attcttttga tttataaggg attttgccga tttcggtcta ttggttaaaa aatgagctga
     4861 tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt ttatggtgca
     4921 ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac
     4981 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     5041 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac
     5101 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     5161 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     5221 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     5281 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     5341 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     5401 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     5461 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     5521 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     5581 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     5641 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     5701 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     5761 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     5821 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     5881 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     5941 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     6001 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     6061 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     6121 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     6181 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     6241 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     6301 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     6361 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     6421 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     6481 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     6541 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     6601 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     6661 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     6721 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     6781 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     6841 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     6901 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     6961 ggccttttgc tcacatgt
//
