LOCUS       sequence-383256-       11020 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Firefly luciferase promoter expression vector driven only by the 
            promoter element of human SOX17.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11020)
  AUTHORS   Landshammer A, Bolondi A, Kretzmer H, Much C, Buschow R, Rose A, Wu 
            HJ, Mackowiak SD, Braendl B, Giesselmann P, Tornisiello R, Parsi KM,
            Huey J, Mielke T, Meierhofer D, Maehr R, Hnisz D, Michor F, Rinn JL,
            Meissner A
  TITLE     T-REX17 is a transiently expressed non-coding RNA essential for 
            human endoderm formation.
  JOURNAL   Elife. 2023 Jan 31;12:e83077. doi: 10.7554/eLife.83077.
  PUBMED    36719724
REFERENCE   2  (bases 1 to 11020)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2023-01-31"; volume: "12:e83077. doi"; pages: " 
            10.7554/eLife.83077"
COMMENT     Sequence Label: pGL4.15_pSOX17
FEATURES             Location/Qualifiers
     source          1..11020
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      5145..5154
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5151..6800
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     CDS             6804..6923
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     polyA_signal    6975..7096
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6986..7005)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7040..7059
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        7290..7647
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7290..7481
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7313..7333)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7498..7633
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7678..8715
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     EVGRV"
     RBS             8694..8702
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    8739..8787
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(8943..8960)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9114..9702)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9194..9213)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9902..10693,10694..10762))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    10867..10915
                     /note="synthetic polyadenylation signal"
     misc_feature    10929..11020
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     10969..10988
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tataacaaaa tgcacacctt
       61 gccctaacca tctggcacaa tccagccctg cttaccaggg aaacttggca agccagagtt
      121 ttcagggtgg aaaggaaacc tattatctct actagaaaaa aaaatctcaa agcgcttgac
      181 ctactgatgt cagcatcacc tttacatgcc aggaacaaat tattacagaa gaccatacct
      241 agaacaccca gctggaagtt aatgaatcag ggaggaagac taatgacacg gcacggtaca
      301 ttcctccgag gcagggaccg cgtctattct ccttcgggtg ggagtggaag tgctctgcgc
      361 acagtgggca cgctgctgat aaggctgtct ggtcaggtga ggacacaggt ctccaaggaa
      421 agccctgcct tctcctaggg tgtggatggg gaaagtggga gtaggagttc acctgcagca
      481 ccgagtgcat agaagctctg ccgctattga aatccctttt aaggctgagg ggcagatctg
      541 aaaggggtga gcaagacagt actgtgtatg tgacgagtcc tgggtgagga acgggaccct
      601 ctccctcatt cccaacgcca cctctccctg gagtatgagg ttccacagcc caggcaataa
      661 agggaagtag cccacacacc tcaacccggg gccacagttg gaggtgggga gatctaagaa
      721 acactccacg gatgagtttc tgaagaggga agtggaccgg gaacagcgca tttcctgctt
      781 cgcctgtgca aatcccaggc ggggccaagc gggaacgggg acacggtcta agagtcgccc
      841 gctgccaggg aggggttggg gggcagcctc gaggggacac cgcggaacca gggagggatg
      901 cgcatggagg gagtgggaga aggcgtccaa gtaaaatgca tgctccggtc atcgggggta
      961 acgcgcgtcc ccaaatcccc tctcgctgtc gccctttttt tcagtcctca cggggagagg
     1021 ccggcgcagc agtggagcac cgcaggggct ggatcggacc cgcactgcag catctgcagg
     1081 gctccgacct ggtgcgggcc tccaaggcag ggggcgcgcg gcggcacagc cggtggccgc
     1141 gattttccgg gccactcctt ccccccttac actcccctcc cgcccgcgac cttcgacctc
     1201 catcctggac ccacggggta ctggcccggg gacaaggagg agacgttaaa ggtatcaagg
     1261 ggcgcggcac cgccgcctag acggcgccgg gacgcgaggt gcgaatcggc ggcgtccggc
     1321 tgcgctgccc tacctgggag gtgcgggacc ctcggccgcg ctccaccgcc gcgcgccacc
     1381 tcgtgctggc ctccgggagg cagccgcccg cgcacgattt ataaatgcaa aggccttatt
     1441 gttgttcttt gttcagggat accattgtct agtcatccac gggcagcctg gagttttccc
     1501 agggtttgcg gcgcgcactc ccctcccccg cccccacaga cttgctctcc agcgccaacc
     1561 cggatctgcc taggtgccca cgggtccttc ccgatcgact tgagtgtcct tcctccagcc
     1621 cctgctttcc cagcatcgaa aacacaaaag cctgccggaa atcacccagc ctttgcctgg
     1681 tcactcaggg gcccacggat aagcgtggct ctgattcctc caggtcagcc ccacggttcg
     1741 ccacctaccc gagttaattt ctgcgcccgc tgaccaaagg ccccaactgg caacgggcac
     1801 agcttcttgg tgcgctagtc gcttccttgc ccagccttta gagcctcggg tccggcccaa
     1861 ggacccgggg acgtgagcgc aattagtagc caggagccct gcgaccctgg accgtgcagt
     1921 gctaacgcac ccgaacggct atgcgagcgg tgtactcggc tcttacttgt gaaagttgtt
     1981 tcccccaacc ccctgcgtaa atctcttgtg ttgagaaaag caaggcttgc aggaagccac
     2041 tccggatgtt tgctgcttta catgtggcat ctgtggctgg ctctgataac gtcccggggc
     2101 accacgtatt aaagtgaaga gactgaaata gaaggctcca cacaccgcgc gcgggtcagc
     2161 gacgtcgccg ccctccacac tgccccccga aatataagac aagcgaatga tcagaagaaa
     2221 tgagatctaa ctaggtcaca gtatgtaaaa gggactagtc ttggaaagcc accacaattc
     2281 ctcccctgta aatttgtaaa ctgtgaattt atgatctcag ggagggtgtt ataagaagta
     2341 gggggagggg cgtcaaaatc tgaagctcct actccaacct atgattgtgt actttcacct
     2401 gatttctctt accagaccaa gatcagtccc caagaacaag ggcaaataaa aatagggaaa
     2461 cccggccggg cgcggtggct cacgacggta atcccagcac tttgggagac caaggcgggc
     2521 ggatcacctg aggtcaggag tttgagacca gcctgaccaa tatgatgaaa ccccgtctct
     2581 actaaaaata caaaaattag ctaggcgtgg tggcatgcgt ctgtaatccc agctactcgg
     2641 gaggctgaga caggagaatc gcttgaatcg gggaggcgga ggttgcagcg agccgagatc
     2701 gcgccattgc actccagcct gggcaacaag agggaaactc tgtctcaata aatgaataag
     2761 taaataaatc aataaaatat ggaaacccca cgttttcttc cccatcattt tctccacctt
     2821 cttggctccc aagcgcacct ctcccaataa tgagctgggt gaggaagagc atgaggatga
     2881 agaaagtgta cagggctaaa tctgtatatg tacatttttt ggactcacac ctggattgta
     2941 tttcctgtag tgttttttaa ggaattagtc aaacagacta gacagataaa aatgaattct
     3001 ggaccagata ttgtttttta attttcaatg tttattctag gaaaactcct tagctcataa
     3061 aagatttagt gttaagagaa aattctgctt tgagagcttt gaaaaatatt tattattatc
     3121 cagaaaagtt agaaaagatc tcaaaaaaaa aaaaaaaaaa aaaacagcaa aaaacaaggt
     3181 actacggggt taatttgtag attgctctct ctcctcctat agttttgttg cttatttaat
     3241 catttgcaat gtgcttttgc tttatactga agcccatttt tttttcagaa aaaataaagc
     3301 tttaaaagta catcatcatt taaatttgtc taaaatcaac cgctttcaaa actaccaact
     3361 agaagtaaca cctctctatg atttctttat gcagatttaa ttttttttct tgtttgaatc
     3421 ttagagaccc aggaactaga aacactttct taacttgtcc cctagccaag gcttcacaga
     3481 tacttgcaga attaaagata agttggctta aaacaattgt gtaagagttc ttttgagaaa
     3541 aagcaatgcc agagaaactg aaggccagga gtttgcaaaa gttttgtatt tttatggatg
     3601 ccactaataa gcaagactgg acagacggtc ttgttgttgt agtatcacac tcaaagcagc
     3661 agcattttac ttctcagatt ccaggtgtaa gctctacttt tttatcacgc cttcataata
     3721 aagaaaatct gttctggaga ttcgtatatt gatttctttg caaagttatc tctaattatg
     3781 caggaatggc tgttagttgc cctcaaaaac caacacaaat acacaaacac aacagcctgg
     3841 cttagtgatt attaagagct gcaagtctga aggcggactg ctgggtgtga attccacctt
     3901 ctcaacgctg tagtctccag caagttcctt aacttctctg cctccgtttc ctttcctgta
     3961 agatagagtc tacctcaggg ttaatatgag gatatgcttc tttgctaatg ctggagggcg
     4021 ctaggaatag cagtggcagg ctgtgtcatt tctcagtgtt gtataacggc ttgttttaaa
     4081 agtcccactg aaaacttttt ttttttccag atcgaaacac aagtttaata gtcattcaga
     4141 tttcattcca gtctaaagaa acttatagaa atctaattat ctgccagaat aaccgcttta
     4201 aactgactgt aagaaccaca gttactttgc agctaaaaca gattgttctc ttcccccaaa
     4261 caaactcgga catttagaca aatattgctg ctgtctttac attttagaaa tcagtattaa
     4321 ggctcacctg ttactgaaca catacgagaa acagttttgt tatgcgtatc ttatatacat
     4381 catgtataca ttttcgtact ttcacatgat gtgtataata agatacacat aaaaactggt
     4441 tggcttgtca tgagtaaatg ctttattctt tagagtgaag gaatattgga tacagaacgt
     4501 cgttccatat ttaatcttgc tttagaaaat taggatttat cttggacaca gcattttgaa
     4561 tataacacag gcaagttgag tcctggggga aaaattgtat ttttaaattt attatcaacg
     4621 gtgtctttcg catttgagaa aacacatttg aagatgccca tgcagttcct tggaagtaaa
     4681 ctaggtcacc caccactgaa acaccctcgg cttctgcaaa aagatgtgct ttaaagagaa
     4741 tagaatggac gctcggtatg ttcatctaaa cgaccttggg caagtacgtc gattccaagg
     4801 tacaatcagc cctcccagac ggcttttcga gtctccctaa ccccggtggg agaggacgcg
     4861 gcgccagagc ccagctccgg ctagttttcc cgggggcagg tgtagccttg ggcgcggggc
     4921 cgggggaggg gcaaggggcg ggcgtggggt tggactggga cgtgggactc ggaccacggc
     4981 ctgggcgtgg gcctaacgac gcgggaccgg cccgccctcg ccgctccatt ggccacatct
     5041 gtgcagaaaa ggccccgcgg cccaggggcg cccgcagtgt cactaggccg gcatcaagat
     5101 ctggcctcgg cggccaagct tggcaatccg gtactgttgg taaagccacc atggaagatg
     5161 ccaaaaacat taagaagggc ccagcgccat tctacccact cgaagacggg accgccggcg
     5221 agcagctgca caaagccatg aagcgctacg ccctggtgcc cggcaccatc gcctttaccg
     5281 acgcacatat cgaggtggac attacctacg ccgagtactt cgagatgagc gttcggctgg
     5341 cagaagctat gaagcgctat gggctgaata caaaccatcg gatcgtggtg tgcagcgaga
     5401 atagcttgca gttcttcatg cccgtgttgg gtgccctgtt catcggtgtg gctgtggccc
     5461 cagctaacga catctacaac gagcgcgagc tgctgaacag catgggcatc agccagccca
     5521 ccgtcgtatt cgtgagcaag aaagggctgc aaaagatcct caacgtgcaa aagaagctac
     5581 cgatcataca aaagatcatc atcatggata gcaagaccga ctaccagggc ttccaaagca
     5641 tgtacacctt cgtgacttcc catttgccac ccggcttcaa cgagtacgac ttcgtgcccg
     5701 agagcttcga ccgggacaaa accatcgccc tgatcatgaa cagtagtggc agtaccggat
     5761 tgcccaaggg cgtagcccta ccgcaccgca ccgcttgtgt ccgattcagt catgcccgcg
     5821 accccatctt cggcaaccag atcatccccg acaccgctat cctcagcgtg gtgccatttc
     5881 accacggctt cggcatgttc accacgctgg gctacttgat ctgcggcttt cgggtcgtgc
     5941 tcatgtaccg cttcgaggag gagctattct tgcgcagctt gcaagactat aagattcaat
     6001 ctgccctgct ggtgcccaca ctatttagct tcttcgctaa gagcactctc atcgacaagt
     6061 acgacctaag caacttgcac gagatcgcca gcggcggggc gccgctcagc aaggaggtag
     6121 gtgaggccgt ggccaaacgc ttccacctac caggcatccg ccagggctac ggcctgacag
     6181 aaacaaccag cgccattctg atcacccccg aaggggacga caagcctggc gcagtaggca
     6241 aggtggtgcc cttcttcgag gctaaggtgg tggacttgga caccggtaag acactgggtg
     6301 tgaaccagcg cggcgagctg tgcgtccgtg gccccatgat catgagcggc tacgttaaca
     6361 accccgaggc tacaaacgct ctcatcgaca aggacggctg gctgcacagc ggcgacatcg
     6421 cctactggga cgaggacgag cacttcttca tcgtggaccg gctgaagagc ctgatcaaat
     6481 acaagggcta ccaggtagcc ccagccgaac tggagagcat cctgctgcaa caccccaaca
     6541 tcttcgacgc cggggtcgcc ggcctgcccg acgacgatgc cggcgagctg cccgccgcag
     6601 tcgtcgtgct ggaacacggt aaaaccatga ccgagaagga gatcgtggac tatgtggcca
     6661 gccaggttac aaccgccaag aagctgcgcg gtggtgttgt gttcgtggac gaggtgccta
     6721 aaggactgac cggcaagttg gacgcccgca agatccgcga gattctcatt aaggccaaga
     6781 agggcggcaa gatcgccgtg aattctcacg gcttccctcc cgaggtggag gagcaggccg
     6841 ccggcaccct gcccatgagc tgcgcccagg agagcggcat ggatagacac cctgctgctt
     6901 gcgccagcgc caggatcaac gtctaaggcc gcgactctag agtcggggcg gccggccgct
     6961 tcgagcagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg
     7021 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
     7081 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga
     7141 ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtaaaa tcgataagga
     7201 tccgtttgcg tattgggcgc tcttccgctg atctgcgcag caccatggcc tgaaataacc
     7261 tctgaaagag gaacttggtt agctaccttc tgaggcggaa agaaccagct gtggaatgtg
     7321 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     7381 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     7441 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     7501 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     7561 atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc
     7621 ttttttggag gcctaggctt ttgcaaaaag ctcgattctt ctgacactag cgccaccatg
     7681 aagaagcccg aactcaccgc taccagcgtt gaaaaatttc tcatcgagaa gttcgacagt
     7741 gtgagcgacc tgatgcagtt gtcggagggc gaagagagcc gagccttcag cttcgatgtc
     7801 ggcggacgcg gctatgtact gcgggtgaat agctgcgctg atggcttcta caaagaccgc
     7861 tacgtgtacc gccacttcgc cagcgctgca ctacccatcc ccgaagtgtt ggacatcggc
     7921 gagttcagcg agagcctgac atactgcatc agtagacgcg cccaaggcgt tactctccaa
     7981 gacctccccg aaacagagct gcctgctgtg ttacagcctg tcgccgaagc tatggatgct
     8041 attgccgccg ccgacctcag tcaaaccagc ggcttcggcc cattcgggcc ccaaggcatc
     8101 ggccagtaca caacctggcg ggatttcatt tgcgccattg ctgatcccca tgtctaccac
     8161 tggcagaccg tgatggacga caccgtgtcc gccagcgtag ctcaagccct ggacgaactg
     8221 atgctgtggg ccgaagactg tcccgaggtg cgccacctcg tccatgccga cttcggcagc
     8281 aacaacgtcc tgaccgacaa cggccgcatc accgccgtaa tcgactggtc cgaagctatg
     8341 ttcggggaca gtcagtacga ggtggccaac atcttcttct ggcggccctg gctggcttgc
     8401 atggagcagc agactcgcta cttcgagcgc cggcatcccg agctggccgg cagccctcgt
     8461 ctgcgagcct acatgctgcg catcggcctg gatcagctct accagagcct cgtggacggc
     8521 aacttcgacg atgctgcctg ggctcaaggc cgctgcgatg ccatcgtccg cagcggggcc
     8581 ggcaccgtcg gtcgcacaca aatcgctcgc cggagcgcag ccgtatggac cgacggctgc
     8641 gtcgaggtgc tggccgacag cggcaaccgc cggcccagta cacgaccgcg cgctaaggag
     8701 gtaggtcgag tttaaactct agaaccggtc atggccgcaa taaaatatct ttattttcat
     8761 tacatctgtg tgttggtttt ttgtgtgttc gaactagatg ctgtcgaccg atgcccttga
     8821 gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac
     8881 ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg ctcttccgct
     8941 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     9001 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     9061 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     9121 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     9181 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     9241 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     9301 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     9361 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     9421 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     9481 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     9541 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     9601 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     9661 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     9721 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     9781 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     9841 taaagtatat atgagtaaac ttggtctgac agcggccgca aatgctaaac cactgcagtg
     9901 gttaccagtg cttgatcagt gaggcaccga tctcagcgat ctgcctattt cgttcgtcca
     9961 tagtggcctg actccccgtc gtgtagatca ctacgattcg tgagggctta ccatcaggcc
    10021 ccagcgcagc aatgatgccg cgagagccgc gttcaccggc ccccgatttg tcagcaatga
    10081 accagccagc agggagggcc gagcgaagaa gtggtcctgc tactttgtcc gcctccatcc
    10141 agtctatgag ctgctgtcgt gatgctagag taagaagttc gccagtgagt agtttccgaa
    10201 gagttgtggc cattgctact ggcatcgtgg tatcacgctc gtcgttcggt atggcttcgt
    10261 tcaactctgg ttcccagcgg tcaagccggg tcacatgatc acccatatta tgaagaaatg
    10321 cagtcagctc cttagggcct ccgatcgttg tcagaagtaa gttggccgcg gtgttgtcgc
    10381 tcatggtaat ggcagcacta cacaattctc ttaccgtcat gccatccgta agatgctttt
    10441 ccgtgaccgg cgagtactca accaagtcgt tttgtgagta gtgtatacgg cgaccaagct
    10501 gctcttgccc ggcgtctata cgggacaaca ccgcgccaca tagcagtact ttgaaagtgc
    10561 tcatcatcgg gaatcgttct tcggggcgga aagactcaag gatcttgccg ctattgagat
    10621 ccagttcgat atagcccact cttgcaccca gttgatcttc agcatctttt actttcacca
    10681 gcgtttcggg gtgtgcaaaa acaggcaagc aaaatgccgc aaagaaggga atgagtgcga
    10741 cacgaaaatg ttggatgctc atactcgtcc tttttcaata ttattgaagc atttatcagg
    10801 gttactagta cgtctctcaa ggataagtaa gtaatattaa ggtacgggag gtattggaca
    10861 ggccgcaata aaatatcttt attttcatta catctgtgtg ttggtttttt gtgtgaatcg
    10921 atagtactaa catacgctct ccatcaaaac aaaacgaaac aaaacaaact agcaaaatag
    10981 gctgtcccca gtgcaagtgc aggtgccaga acatttctct
//
