LOCUS       sequence-383260-       13471 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of mCherry tagged B1 Integrin. Autoclustering 
            mutant..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13471)
  AUTHORS   Paszek MJ, Zahir N, Johnson KR, Lakins JN, Rozenberg GI, Gefen A, 
            Reinhart-King CA, Margulies SS, Dembo M, Boettiger D, Hammer DA, 
            Weaver VM
  TITLE     Tensional homeostasis and the malignant phenotype.
  JOURNAL   Cancer Cell. 2005 Sep;8(3):241-54. doi: 10.1016/j.ccr.2005.08.010.
  PUBMED    16169468
REFERENCE   2  (bases 1 to 13471)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccr.2005.08"; journalName: "Cancer Cell"; date: 
            "2005-09"; volume: "8"; issue: "3"; pages: "241-54"
COMMENT     Sequence Label: B1-INTEGRIN VN
FEATURES             Location/Qualifiers
     source          1..13471
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(72..1250)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          81..1019
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(104..124)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             complement(1633..1677)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1862..2095
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2588..2713
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             2760..3393
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    3561..3695
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3585..3604)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3639..3658
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(4120..4140)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          4270..4335
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     promoter        complement(5469..5798)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5483..5618
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5783..5803
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     complement(5873..5890)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6044..6632)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6124..6143)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6803..7594,7595..7663))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7426..7445
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7664..7768)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7836..7854
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7892..7914)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8014..8033
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8247..8266
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        8556..8574
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     8556..8573
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     LTR             8599..8779
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     protein_bind    8799..8832
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    8976..9564
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             9082..9093
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     9491..9511
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9573..10283)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(9851..9870)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     10120..10138
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     misc_feature    join(10284..10292,10293..10295,10296..10870)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(10457..10476)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     10686..10703
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     misc_feature    join(13378..13471,1..24)
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ccaatccccc cttttctttt aaaagttaac cgataccgtc gagattaatt aaatttaaat
       61 gtcgaaattc ctcacgacac ctgaaatgga agaaaaaaac tttgaaccac tgtctgaggc
      121 ttgagaatga accaagatcc aaactcaaaa agggcaaatt ccaaggagaa ttacatcaag
      181 tgccaagctg gcctaacttc agtctccacc cactcagtgt ggggaaactc catcgcataa
      241 aacccctccc cccaacctaa agacgacgta ctccaaaagc tcgagaacta atcgaggtgc
      301 ctggacggcg cccggtactc cgtggagtca catgaagcga cggctgagga cggaaaggcc
      361 cttttccttt gtgtgggtga ctcacccgcc cgctctcccg agcgccgcgt cctccatttt
      421 gagctccctg cagcagggcc gggaagcggc catctttccg ctcacgcaac tggtgccgac
      481 cgggccagcc ttgccgccca gggcggggcg atacacggcg gcgcgaggcc aggcaccaga
      541 gcaggccggc cagcttgaga ctacccccgt ccgattctcg gtggccgcgc tcgcaggccc
      601 cgcctcgccg aacatgtgcg ctgggacgca cgggccccgt cgccgcccgc ggccccaaaa
      661 accgaaatac cagtgtgcag atcttggccc gcatttacaa gactatcttg ccagaaaaaa
      721 agcgtcgcag caggtcatca aaaattttaa atggctagag acttatcgaa agcagcgaga
      781 caggcgcgaa ggtgccacca gattcgcacg cggcggcccc agcgcccagg ccaggcctca
      841 actcaagcac gaggcgaagg ggctccttaa gcgcaaggcc tcgaactctc ccacccactt
      901 ccaacccgaa gctcgggatc aagaatcacg tactgcagcc aggtggaagt aattcaaggc
      961 acgcaagggc cataacccgt aaagaggcca ggcccgcggg aaccacacac ggcacttacc
     1021 tgtgttctgg cggcaaaccc gttgcgaaaa agaacgttca cggcgactac tgcacttata
     1081 tacggttctc ccccaccctc gggaaaaagg cggagccagt acacgacatc actttcccag
     1141 tttaccccgc gccaccttct ctaggcaccg gttcaattgc cgacccctcc ccccaacttc
     1201 tcggggactg tgggcgatgt gcgctctgcc cactgacggg caccggagcc aattcccact
     1261 cctttcaaga cctagaaggt ccattagctg caaagattcc tctctgttta aaactttatc
     1321 catctttgca aagcttatcg tcgacatcga tggtcgaggc tagtctcgtg atcgataccg
     1381 tcgagatccg ttcactaatc gaatggatct gtctctgtct ctctctccac cttcttcttc
     1441 tattccttcg ggcctgtcgg gtcccctcgg ggttgggagg tgggtctgaa acgataatgg
     1501 tgaatatccc tgcctaactc tattcactat agaaagtaca gcaaaaacta ttcttaaacc
     1561 taccaagcct cctactatca ttatgaataa ttttatatac cacagccaat ttgttatgtt
     1621 aaaccaattc cacaaacttg cccatttatc taattccaat aattcttgtt cattcttttc
     1681 ttgctggttt tgcgattctt caattaagga gtgtattaag cttgtgtaat tgttaatttc
     1741 tctgtcccac tccatccagg tcgtgtgatt ccaaatctgt tccagagatt tattactcca
     1801 actagcattc caaggcacag cagtggtgca aatgagtttt ccagagcaac cccaaatccc
     1861 caggagctgt tgatccttta ggtatctttc cacagccagg attcttgcct ggagctgctt
     1921 gatgccccag actgtgagtt gcaacagatg ctgttgcgcc tcaatagccc tcagcaaatt
     1981 gttctgctgc tgcactatac cagacaataa ttgtctggcc tgtaccgtca gcgtcattga
     2041 cgctgcgccc atagtgcttc ctgctgctcc caagaaccca aggaacaaag ctcctattcc
     2101 cactgctctt ttttctctct gcaccactct tctctttgcc ttggtgggtg ctactcctaa
     2161 tggttcaatt tttactactt tatatttata taattcactt ctccaattgt ccctcatatc
     2221 tcctcctcca ggtctgaaga tcagcggccg cttgctgtgc ggtggtctta cttttgtttt
     2281 gctcttcctc tatcttgtct aaagcttcct tggtgtcttt tatctctatc ctttgatgca
     2341 cacaatagag ggttgctact gtattatata atgatctaag ttcttctgat cctgtctgaa
     2401 gggatggttg tagctgtccc agtatttgtc tacagccttc tgatgtttct aacaggccag
     2461 gattaactgc gaatcgttct agctccctgc ttgcccatac tatatgtttt aatttatatt
     2521 ttttctttcc ccctggcctt aaccgaattt tttcccatcg cgatctaatt ctcccccgct
     2581 taatactgac gctctcgcac ccatctctct ccttctagcc tccgctagtc aaaatttttg
     2641 gcgtactcac cagtcgccgc ccctcgcctc ttgccgtgcg cgcttcagca agccgagtcc
     2701 tgcgtcgaga gagctcctct ggtttccctt tcgctttcaa gtccctgttc gggcgccact
     2761 gctagagatt ttccacactg actaaaaggg tctgagggat ctctagttac cagagtcaca
     2821 caacagacgg gcacacacta cttgaagcac tcaaggcaag ctttattgag gcttaagcag
     2881 tgggttccct agttagccag agagctccca ggctcagatc tggtctaacc agagagaccc
     2941 agtacaggca aaaagcagct gcttatatgc aggatctgag ggctcgccac tccccagtcc
     3001 cgcccaggcc acgcctccct ggaaagtccc cagcggaaag tcccttgtag caagctcgat
     3061 atcagcagtt cttgaagtac tccggatgca gctctcgggc cacgtgatga aatgctaggc
     3121 ggctgtcaaa cctccactct aacacttctc tctccgggtc atccatccca tgcaggctca
     3181 cagggtgtaa caagctggtg ttctctcctt tattggcctc ttctacctta tctggctcaa
     3241 ctggtactag cttgtagcac catccaaagg tcagtggata tctgacccct ggccctggtg
     3301 tgtagttctg ctaatcaggg aagtagcctt gtgtgtggta gatccacaga tcaaggatat
     3361 cttgtcttct ttgggagtga attagccctt ccaactacta agtttgtagt acatatttaa
     3421 caaatacaat ttctttaaaa tgaaaataat tcagaggaat cacaggttta gagtaaatga
     3481 aaccacaggt aattggcagt ggtaataggg tatggggtgg gaagtttggg atgattttgg
     3541 ttagcttgag ttatccagtt gatccagaca tgataagata cattgatgag tttggacaaa
     3601 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt
     3661 tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta
     3721 tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat
     3781 gtggtatggc tgattatgat ctctagtcaa ggcactatac atcaaatatt ccttattaac
     3841 ccctttacaa attaaaaagc taaaggtaca caatttttga gcatagttat taatagcaga
     3901 cactctatgc ctgtgtggag taagaaaaaa cagtatgtta tgattataac tgttatgcct
     3961 acttataaag gttacagaat atttttccat aattttcttg tatagcagtg cagctttttc
     4021 ctttgtggtg taaatagcaa agcaagcaag agttctatta ctaaacacag catgactcaa
     4081 aaaacttagc aattctgaag gaaagtcctt ggggtcttct acctttctct tcttttttgg
     4141 aggagtagaa tgttgagagt cagcagtagc ctcatcatca ctagatggca tttcttctga
     4201 gcaaaacagg ttttcctcat taaaggcatt ccaccactgc tcccattcat cagttccata
     4261 ggttggaatc taaaatacac aaacaattag aatcagtagt ttaacacatt atacacttaa
     4321 aaattttata tttaccttag agctttaaat ctctgtaggt agtttgtcca attatgtcac
     4381 accacagaag taaggttcct tcacaaagat ccggggccca ctcataaatc cagttgccgc
     4441 cacggtagcc aatcaccgta tcgtataaat catcgtcggt acgttcggca tcgctcatca
     4501 caatacgtgc ctggacgtcg aggatttcgc gtgggtcaat gccgcgccag atccacatca
     4561 gacggttaat catgcgatac cagtgaggga tggttttacc atcaagggcc gactgcacag
     4621 gcggttgtgc gccgtgatta aagcggcgga ctagcgtcga ggtttcagga tgtttaaagc
     4681 ggggtttgaa cagggtttcg ctcaggtttg cctgtgtcat ggatgcagcc tccagaatac
     4741 ttactggaaa ctattgtaac ccgcctgaag ttaaaaagaa caacgcccgg cagtgccagg
     4801 cgttgaaaag attagcgacc ggagattggc gggacgaata cgacgcccat atcccacggc
     4861 tgttcaatcc aggtatcttg cgggatatca acaacatagt catcaaccag cggacgacca
     4921 gccggttttg cgaagatggt gacaaagtgc gcttttggat acatttcacg aatcgcaacc
     4981 gcagtaccac cggtatccac caggtcatca ataacgatga agccttcgcc atcgccttct
     5041 gcgcgtttca gcactttaag ctcgcgctgg ttgtcgtgat cgtagctgga aatacaaacg
     5101 gtatcgacat gacgaatacc cagttcacgc gccagtaacg cacccggtac agaccgccac
     5161 ggcttacggc aataatgcct ttccattgtt cagaaggcat cagtcggctt gcgagtttac
     5221 gtgcatggat ctgcaacatg tcccaggtga cgatgtattt ttcgctcatg tgaagtgtcc
     5281 cagcctgttt atctacggct taaaaagtgt tcgaggggaa aataggttgc gcgagattat
     5341 agagatctgg cgcactaaaa accagtattt cacatgagtc cgcgtctttt tacgcactgc
     5401 ctctccctga cgcgggataa agtggtattc tcaaacatat ctcgcaagcc tgtcttgtgt
     5461 ccaagctttt tgcaaaagcc taggcctcca aaaaagcctc ctcactactt ctggaatagc
     5521 tcagaggccg aggcggcctc ggcctctgca taaataaaaa aaattagtca gccatggggc
     5581 ggagaatggg cggaactggg cggagttagg ggcgggatgg gcggagttag gggcgggact
     5641 atggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg ggagcctggg
     5701 gactttccac acctggttgc tgactaattg agatgcatgc tttgcatact tctgcctgct
     5761 ggggagcctg gggactttcc acaccctaac tgacacacat tccacagctg cattaatgaa
     5821 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     5881 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     5941 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     6001 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     6061 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     6121 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     6181 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     6241 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     6301 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     6361 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     6421 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     6481 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     6541 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     6601 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     6661 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     6721 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     6781 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     6841 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     6901 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     6961 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     7021 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     7081 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     7141 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     7201 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     7261 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     7321 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     7381 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     7441 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     7501 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     7561 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     7621 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     7681 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     7741 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
     7801 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
     7861 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     7921 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     7981 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag
     8041 tgcaccattc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg
     8101 ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac
     8161 agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg
     8221 aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca
     8281 cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatctg gctagcgatg
     8341 accctgctga ttggttcgct gaccatttcc gggtgcggga cggcgttacc agaaactcag
     8401 aaggttcgtc caaccaaacc gactctgacg gcagtttacg agagagatga tagggtctgc
     8461 ttcagtaagc cagatgctac acaattaggc ttgtacatat tgtcgttaga acgcggctac
     8521 aattaataca taaccttatg tatcatacac atacgattta ggtgacacta tagaatacac
     8581 ggaattaatt ctagatgctg ctagagattt tccacactga ctaaaagggt ctgagggatc
     8641 tctagttacc agagtcacac aacagacggg cacacactac ttgaagcact caaggcaagc
     8701 tttattgagg cttaagcagt gggttcccta gttagccaga gagctcccag gctcagatct
     8761 ggtctaacca gagagaccca gtacaagcaa aaagcagcat aacttcgtat aatgtatgct
     8821 atacgaagtt attcccatgg tgaattgatc ccatcttgtc ttcgttggga gtgaattagc
     8881 ccttccagtc cccccttttc ttttaaaaag tggctaagat ctacagctgc cttgtaagtc
     8941 attggtctta aaggtacctg gccatacgta ccgatgcggg gaggcggccc aaagggagat
     9001 ccgactcgtc tgagggcgaa ggcgaagacg cggaagaggc cgcagagccg gcagcaggcc
     9061 gcgggaagga aggtccgctg gattgagggc cgaagggacg tagcagaagg acgtcccgcg
     9121 cagaatccag gtggcaacac aggcgagcag ccatggaaag gacgtcagct tccccgacaa
     9181 caccacggaa ttgtcagtgc ccaacagccg agcccctgtc cagcagcggg caaggcaggc
     9241 ggcgatgagt tccgccgtgg caatagggag ggggaaagcg aaagtcccgg aaaggagctg
     9301 acaggtggtg gcaatgcccc aaccagtggg ggttgcgtca gcaaacacag tgcacaccac
     9361 gccacgttgc ctgacaacgg gccacaactc ctcataaaga gacagcaacc aggatttata
     9421 caaggaggag aaaatgaaag ccatacggga agcaatagca tgatacaaag gcattaaagc
     9481 agcgtatcca catagcgtaa aaggagcaac atagttaaga ataccagtca atctttcaca
     9541 aattttgtaa tccagaggtt gattatcata tgttacttgt acagctcgtc catgccgccg
     9601 gtggagtggc ggccctcggc gcgttcgtac tgttccacga tggtgtagtc ctcgttgtgg
     9661 gaggtgatgt ccaacttgat gttgacgttg taggcgccgg gcagctgcac gggcttcttg
     9721 gccttgtagg tggtcttgac ctcagcgtcg tagtggccgc cgtccttcag cttcagcctc
     9781 tgcttgatct cgcccttcag ggcgccgtcc tcggggtaca tccgctcgga ggaggcctcc
     9841 cagcccatgg tcttcttctg cattacgggg ccgtcggagg ggaagttggt gccgcgcagc
     9901 ttcaccttgt agatgaactc gccgtcctgc agggaggagt cctgggtcac ggtcaccacg
     9961 ccgccgtcct cgaagttcat cacgcgctcc cacttgaagc cctcggggaa ggacagcttc
    10021 aagtagtcgg ggatgtcggc ggggtgcttc acgtaggcct tggagccgta catgaactga
    10081 ggggacagga tgtcccaggc gaagggcagg gggccaccct tggtcacctt cagcttggcg
    10141 gtctgggtgc cctcgtaggg gcggccctcg ccctcgccct cgatctcgaa ctcgtggccg
    10201 ttcacggagc cctccatgtg caccttgaag cgcatgaact ccttgatgat ggccatgtta
    10261 tcctcctcgc ccttgctcac catggttgtg gccatattat catcgtgttt ttcaaaggaa
    10321 aaccacgtcc ccgtggttcg gggggcctag acgttttttt aacctcgact aaacacatgt
    10381 aaagcatgtg taccgaggcc ccagatcaga tcccatacaa tggggtacct tctgggcatc
    10441 cttcagcccc ttgttgaata cgcttgagga gagccatttg actctttcca caactatcca
    10501 actcacaacg tggcactggg gttgtgccgc ctttgcaggt gtatcttata cacgtggctt
    10561 ttggccgcag aggcacctgt cgccaggtgg ggggttccgc tgcctgcaaa gggtcgctac
    10621 agacgttgtt tgtcttcaag aagcttccag aggaactgct tccttcacga cattcaacag
    10681 accttgcatt cctttggcga gaggggaaag acccctagga atgctcgtca agaagacagg
    10741 gccaggtttc cgggccctca cattgccaaa agacggcaat atggtggaaa ataacatata
    10801 gacaaacgca caccggcctt attccaagcg gcttcggcca gtaacgttag ggggggggga
    10861 gggagagggg cggatcagcg ggtttaaacg ggccctctag actcgaggtc gagtactcat
    10921 tttccctcat acttcggatt gaccacagtt gttacggcac tcttataaat aggattttca
    10981 cccgtgtccc atttggcatt cattttctcc ttttcaaatt tagcaaactc ccttctgtca
    11041 tgaattatca ttaaaagctt ccatatcagc agtaatgcaa ggccaataag aacaattcca
    11101 gcgttaacac cagctacaat tggaatgatg tctggaccag tgggacactc tggattctcc
    11161 acaacatgaa ccatgacctc gttgttccca ttcactgaat acgtaaaata gaaccaacag
    11221 tcgtcaacat ccttctcctt acaatgggac acaggatcag gttggaccgg ctggggtaat
    11281 ttgtcccgac tttctacctt ggtaatgtta aaataggaac attcctgtgt gcatgtgtct
    11341 ttcttttctc ctttattgaa ggctctgcac tgaacacatt ctttatgctc agcacagaca
    11401 ccaaggcagg tctgacacat ctcacacgtt tgcccttgaa acttcggatc tgtacactta
    11461 cagacaccac actcgcagat gccccggcca ttgcagatct gtccgttgct ggcttcacaa
    11521 gtactagtat ccaaagaaca gtcacatgca ctgccagtgt agttggggtt gcactcacac
    11581 acacgacact tgcaaacacc atttcctcca caaattaagc cattggatct atcacagttg
    11641 aaattatcac actcgcagaa tttgccagaa taaatttcat ttgtattatc cctcttccta
    11701 caaacacact gtccgcagac gcactctcca ttgttactgc agatttctga actgttttct
    11761 ttcctgcagt aagcatccat gtcttcactg ttaacttcat ctgtgctgca ttcacaatgt
    11821 ctaccaacac gcccttcatt gcacctgcac gcgccacact caaatgtccc atttccttca
    11881 tgacacttgg gactttcagg gatgccttcg ctttggcatt cacattcaca gatgtactga
    11941 agaataacct ctacttcctc cgtaaagccc agaggcctaa ttttaaagct gtcagaatcc
    12001 ttttttggac acttatttga agttatgcta atttcaaatt gaacctcatc tccaatggaa
    12061 atattggaac attttcttcc attttcccct gttccattca ccccgttctt gcagtaagat
    12121 ttgtaactta ttgttacgcc ttctgacaat ttgccgtttt ccaaaatgac ttctgaggaa
    12181 agggaattgt atgcatcaat gatcaactga attacattgc tagaattggc agataatgtt
    12241 cctactgctg acttagggat caagtttttc agctccttgt aaacaggctg aaattcttca
    12301 gtaactgcaa aaattgtctg aatattattt tcactcagtt tctggacaag gtgagcaata
    12361 gaaggataat cataataatg gctcattgtg tacatattat tttccaggtg acattgtcca
    12421 tcatttggta aaacaatgcc accaagtttc ccatctccag caaagtgaaa cccggcatct
    12481 gtggaaaaca ccagcagccg tgtaacattc ctccagccaa tcagtgatcc acaaactgca
    12541 acttgcatga tggcatcgaa accaccttct ggagaatcca aatttccaga tatgcgctgt
    12601 tttccaacaa gttcattaaa tacttctcct ttactagtaa gactgagcac atttttgtag
    12661 ctaaatgggc tggtgcagtt ctgttcactt gtgcaagggt tcctgagctt agctggtgtt
    12721 gtgctaatgt aaggcatcac agtcttttcc acaaatgagc caaatccaat tctgaagtcc
    12781 gaagtaatcc tcctcatttc attcgtcaga tctgttccaa gactttttac attctccaaa
    12841 tcgtctttca ttgaataaga caggtccata aggtagtaga ggtcaatggg atagtcttca
    12901 gctctcttga attttaatgt aaatgtctgt ggctcccctg atcttaatcg caaaaccaac
    12961 tgctgtggtt ggatctgagt aatatcctct ggcttgagct tctctgctgt tcctttgcta
    13021 cggttggtta catttttatt tttctttata tctttggagc ctctgggatt ttctatgtca
    13081 tctggagggc aacccttctt ttttaaggct tctaaatcat cacatcgtgc agaagtaggc
    13141 attccttcct gtaaaaatgt tgaatttgtg caccacccac aatttggccc tgcttgtata
    13201 cattctccac atgatttggc atttgctttt aaacatctat tttcatctgt ttgagcaaac
    13261 acaccgcaaa ctgaactgat cagtccaatc cagaaaattg gttgtaaatt catggtggcg
    13321 gatccgagct cggtaccaag cttaagttta aacctcgagg ctagtctcgt gatcgataaa
    13381 attttgaatt tttgtaattt gtttttgtaa ttctttagtt tgtatgtctg ttgctattat
    13441 gtctactatt ctttcccctg cactgtaccc c
//
